BIOMIMESYS® Adipose tissue represents a new generation of mimetic Hydroscaffold™ for 3D culture of adipocyte and adipocyte-like cells. Available in a ready-to-use format, it enables the culture of adipocytes and adipocyte-like cells under physiological conditions that are representative of the microenvironment found in adipose tissue. The highly porous nature of the scaffold allows the rapid uptake of nutrients, oxygen, etc. into the cells to create a reproducible study model for all downstream analyses used with 3D adipocyte cultures.

1. HCS Pharma – Siège de Lille
Biocentre Fleming Bat A
250 rue Salvador Allende
59120 LOOS
BIOMIMESYS®
is a registered trademark
of HCS Pharma
+33 769 999 137
www.biomimesys.com
hello@biomimesys.com
Innovative Hydroscaffolds™s for 3D Cell Culture to better mimic the tissue microenvironment and provide
more predictive tools for drug development and metabolic studies
Other BIOMIMESYS® products available
PRODUCT
BIOMIMESYS® Adipose tissue represents a new
generation of mimetic Hydroscaffold™ for 3D
culture of adipocyte and adipocyte-like cells.
Available in a ready-to-use format, it enables the
culture of adipocytes and adipocyte-like cells under
physiological conditions that are representative of
the microenvironment found in adipose tissue. The
highly porous nature of the scaffold allows the rapid
uptake of nutrients, oxygen, etc. into the cells to
create a reproducible study model for all
downstream analyses used with 3D adipocyte
cultures.
Hyaluronic acid (HA) being a major component of
the cell’s extracellular matrix (ECM),
biofunctionalized with adipose tissue ECM
Figure 1: SEM picture with artifically stained collagen (in brown)

2. HCS Pharma – Siège de Lille
Biocentre Fleming Bat A
250 rue Salvador Allende
59120 LOOS
BIOMIMESYS®
is a registered trademark
of HCS Pharma
+33 769 999 137
www.biomimesys.com
hello@biomimesys.com
components (Collagens type I and VI and cell binding domain of Fibronectin), BIOMIMESYS® Adipose tissue
Hydroscaffold™ for 3D adipocyte-like cells is thus an ideal study model because it is:
 Highly porous for the simple extraction of RNA and proteins
 Translucent for direct visualization of cells
 Representative of the adipocytes microenvironment in terms of cell-cell interactions, matrix
composition and cell-matrix interactions
 HYALURONIC ACID (HA) + Collagen I & VI system, major component of the adipocyte extracellular
matrix (ECM), where it plays an IMPORTANT ROLE in connective tissue.
 HA-based scaffolds are formed by HA-grafted RGDS + Collagen I & VI system crosslinking HA with
ADH to form reticulated chains.
 High molecular weight HA is made from Gram+bacteria, which makes it reproducible.
 PHYSICOCHEMICAL FEATURES
o Porosity: 70-170um
o Rheology: Young’s modulus: E= 0.45 ± 0.05kPa
o Swelling ratio = 60 ± 10g/g
ADIPOCYTES
The major role of the adipocyte is to store (lipogenesis) and release fat
(lipolysis), an energy reservoir in the body. The phenomena of
lipogenesis and lipolysis can be controlled, among other things, by the
secretion of adipokines from adipose tissue. This secretion can have
autocrine, paracrine or endocrine effects. Deregulation of lipolytic
mechanisms plays a role in many diseases (obesity, diabetes, etc.).
LIPOGENESIS – AN IMPORTANT ROLE OF ADIPOCYTES
Lipogenesis is the process of storing the free fatty acids (FAs) in the form of triglycerides (TGs) within droplets
leading to mature adipocytes. These mature adipocytes are composed of a single lipid vesicle, a nucleus
present at the periphery of the cell and a very thin cytoplasm. If necessary, TGs are hydrolyzed by lipolysis
and glycerol is released (1).

3. HCS Pharma – Siège de Lille
Biocentre Fleming Bat A
250 rue Salvador Allende
59120 LOOS
BIOMIMESYS®
is a registered trademark
of HCS Pharma
+33 769 999 137
www.biomimesys.com
hello@biomimesys.com
Adipogenesis is a phenomenon characterized by chronological changes in the expression of many genes, in
two stages:
1) Proliferation of preadipocytes, examples of early markers:
 Lipoprotein lipase: LPL
 C / EBPα
 PPARγ2
 FABP-4
2) Differentiation: increased
lipogenesis and insulin sensitivity,
markers amongst others:
 Perilipin
 ATPcitratelysase
 Malic enzyme
 AcetylCoAcarboxylase
 LHS
 Glut4 …
CELL CULTURE MIMICKING THE FAT CELL MICROENVIRONMENT
The cell culture capable of mimicking, in vitro, the development of adipose tissue in vivo, is an experimental
field for the study of physiology and function of adipocyte. This study was originally submitted to
investigative challenges on animals or on isolated cells. New cell models also allow the evaluation and drug
screening of therapeutic interest.
Adipocytes differentiated in 2D do not express adipocyte genes in the same way as adipocytes in vivo. The
confluence found in 2D and the lack of cell/cell or cell/extracellular matrix interactions results in a
heterogeneous culture, with a population of undifferentiated adipocytes (fibroblast shape). This model
seems less predictive in the study of diseases such as diabetes or obesity (1).
Cristancho (2), Dalby (3) and Mc Beath (4) have independently shown that a spheroidal cell shape,
accompanied by a physiological composition of the extracellular environment, allows to strongly induce the
differentiation of preadipocytes.
On this basis, HCS Pharma has developed a 3D hydroscaffold, whose composition and organization are
similar to in vivo adipose tissue, by including collagens I, VI and fibronectin (RGDS pattern) in a cross-linked
hyaluronic acid Hydroscaffold™.

4. HCS Pharma – Siège de Lille
Biocentre Fleming Bat A
250 rue Salvador Allende
59120 LOOS
BIOMIMESYS®
is a registered trademark
of HCS Pharma
+33 769 999 137
www.biomimesys.com
hello@biomimesys.com
Figure 3: SEM picture of BIOMIMESYS® Adipose tissue (collagen chains are artificially stained in brown)
In order to demonstrate the induction of adipogenesis by BIOMIMESYS® Adipose tissue, various tests were
performed on two cell types: 3T3-L1 and Human White Preadipocyte (HWP) according to the following
culture protocol.
Protocol: as in 2D culture, seeding and
differentiation steps are induced from
day 0 (for HWP and 3T3-L1) until
complete maturation.
REFERENCES
1. F. Louis et al. A biomimetic hydrogel functionalized with adipose ECM components as la
microenvironment for the 3D culture if human and murine adipocytes. Biotechnol Bioeng.
2017;114:1813-1824
2. Neels JG, Thinnes T, Loskutoff DJ. Angiogenesis in an in vivo model of adipose tissue development.
FASEB J 2004;18:983–985.
Figure 2: BIOMIMESYS® Adipose tissue (left) and decellularized human adipose tissue in vivo (5) (right)

5. HCS Pharma – Siège de Lille
Biocentre Fleming Bat A
250 rue Salvador Allende
59120 LOOS
BIOMIMESYS®
is a registered trademark
of HCS Pharma
+33 769 999 137
www.biomimesys.com
hello@biomimesys.com
3. Ana G. Cristancho & Mitchell A. Laza. Forming functional fat: a growing understanding of adipocyte
differentiation. Nature Reviews Molecular Cell Biology 2011;12, 722-734.
4. Matthew J. Dalby, Nikolaj Gadegaard & Richard O. C. Oreffo. Harnessing nanotopography and
integrin–matrix interactions to influence stem cell fate. Nature Materials 2014;13,558–569.
5. McBeath R, Pirone DM, Nelson CM, Bhadriraju K, Chen CS. Cell shape, cytoskeletal tension, and
RhoA regulate stem cell lineage commitment. Dev Cell. 2004 Apr;6(4):483-95.
6. L. Wang et al. Combining decellularized human adipose tissue extracellular matrix and adipose-
derived stem cells for adipose tissue engineering, Acta Biomaterialia 2013 (9):8921-8931.
PROOF OF CONCEPT
IN VIVO-LIKE MORPHOLOGY OF ADIPOCYTES
INCREASED EXPRESSION OF DIFFERENTIATION GENES
HIGHER LIPOGENESIS ACTIVITY
MATURATION OF FUNCTIONAL HUMAN ADIPOCYTES
APPLICATION: REGULATION OF LIPID ACCUMULATION
READY TO USE
BIOMIMESYS® is EASY to handle & READY-TO-USE. Upon receiving the vacuum sealed 96-well plate, open it
(under a hood) and add the cells directly on top of the matrix. Changing the culture medium is easy as well.
To remove medium, simply draw the medium with a pipette between the matrix and the edge of the well.
To refresh the medium, place fresh medium onto the surface of the matrix.
15-50µl of cell
suspension dropped on
the surface of
BIOMIMESYS®
Culture medium is gently
added on the side of the
well to obtain qs. 200µl

6. HCS Pharma – Siège de Lille
Biocentre Fleming Bat A
250 rue Salvador Allende
59120 LOOS
BIOMIMESYS®
is a registered trademark
of HCS Pharma
+33 769 999 137
www.biomimesys.com
hello@biomimesys.com
COMPATIBLE TECHNOLOGIES
BIOMIMESYS® Hydroscaffold™ has many properties (translucent, porous, and biodegradable) that make it
ideal for use with numerous downstream applications. The growing cells are easily retrieved from the matrix
by a gentle and rapid procedure, this means that cells cultured in BIOMIMESYS® may be analyzed using all
technologies as shown below.
TRANSPARENT POROUS BIODEGRADABLE SOLID
Microscopy PCRs Flow cytometry Histology
Plate Reader (OD,
fluorescence,
luminescence
Western-Blot
ELISA
PRICING
BIOMIMESYS®Adipose tissue is sold for RESEARCH USE ONLY.
96-WELL PLATE
**price per plate**
Quantity of 96-well plates 1 ≥ 10 ≥ 100 ≥ 1000
BIO_ADI_96_96_transp
(96 well plate with 96 Hydroscaffolds™)
BIO_ADI_96_24_transp
(96 well plate with 24 Hydroscaffolds™)
Quantity of 96-well *BLACK*plates 1 ≥ 10 ≥ 100 ≥ 1000
BIO_ADI_96_96_black
(96 well plate with 96 Hydroscaffolds™)
BIO_ADI_96_24_black
(96 well plate with 24 Hydroscaffolds™)
Contact Information
HCS Pharma / hello@biomimesys.com / http://www.biomimesys.com