Importance of matricial and cellular microenvironments in in vitro models for drug discovery in Parkinson’s disease
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There is a 90% failure in clinical trials, due to efficacy and safety issues, which frequently concerns the central nervous system. That points a lack of relevance of preclinical models used upstream. In this frame, the aim of this study was to develop more relevant cerebral in vitro models, by including the matricial and cellular microenvironments, for drug discovery in Parkinson's disease. HD available on https://hcs-pharma.com/poster---importance-of-matricial-and-cellular-microenvironments-in-in-vitro-models-for-drug-discovery-in-parkinsons-disease/
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This document presents a human-in-a-chip system for evaluating antioxidant activity through liver metabolism using a microfluidic device. The system aims to mimic liver metabolism processes and determine how they affect antioxidant compounds' activity. Key aspects include:
1. Encapsulating liver enzymes in microchannels using photolithography to mimic metabolism reactions.
2. Optimizing flow rates using computational models to minimize precipitation and ensure homogeneous mixing.
3. Validating the system by examining reaction kinetics for quercetin scavenging of free radicals on chip, determining a rate constant that matches computational simulations.
The microfluidic human-in-a-chip system aims to study how liver metabolism impacts antioxidant compounds'
Decellularized whartons jelly_from_human_umbilical_cord_as_a_novel_3_d_scaffo...
Decellularized whartons jelly_from_human_umbilical_cord_as_a_novel_3_d_scaffo...ComprehensiveBiologi
Decellularized whartons jelly_from_human_umbilical_cord_as_a_novel_3_d_scaffolding_material_for_tissue_engineering_applicNovel Way to Isolate Adipose Derive Mesenchymal Stem Cells & Its Future Clini...
Abstract: Adipose-derived stem cells (ADSCs), were isolated from discarded human fat tissue, obtained from csection with our recently modified methods, in Stem Cell & Regenerative Medicine Lab, VSBT. Here we develop
two methods to isolate Adipose derived mesenchymal stem cells with enzyme digestion and use of
phosphatidylcholine and deoxycholate. Surface protein expression was analyzed by flow cytometry to
characterize the cell phenotype. The multi-lineage potential of ADSCs was testified by differentiating cells with
adipogenic inducer. ADSCs can be cultured in vitro for up to one month without passage. Also, the flow
cytometry analysis showed that ADSCs expressed high levels of stem cell related surface marker CD105.
ADSCs have strong proliferation ability, maintain their phenotypes, and have stronger multi-differentiation
potential. The molecular basis of ADSC differentiation was studied using bioinformatics tools with an aim to
identify the key proteins involved in differentiation, such that they could be used as potential targets for drug
development for the treatment of obesity. The key proteins involved were found to be PPARG and C/EBPα. The
structures of the proteins were retrieved from MMDB (Molecular Modelling Database) and PDB (Protein Data
Bank) respectively. Key Words: Adipose-derived stem cells, Mesenchymal stem cells, Enzyme digestion, Phosphatidylcholine, Deoxycholate, PPARG, C/EBPα, etc.
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BIOMIMESYS® Liver, a 3D cell culture model for maintaining and promoting hep...
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Ch1 cell in Health and disease Robbins Basic Pathology 10th edition
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Poster - Including the matricial tumoral microenvironment in 3D in vitro mode...
In oncology, 97% of drug candidates fail in clinical trials. This highlights a lack of relevance of preclinical models used upstream. Indeed, human in vitro models don’t consider the Tumoral Extracellular Matrix (TECM). However, more and more studies demonstrate that ECM composition and stiffness are modified in tumors and are linked to cancer initiation, progression, propagation, and drug resistances.
BIOMIMESYS® is a Hyaluronic Acid-based matrix grafted with structural and adhesion molecules, which mimics the ECM/TECM. It is chemically defined and its composition and stiffness can be modified to reproduce the organ-specificity of the ECM, or to mimic a pathological microenvironment in vitro.
We have demonstrated that the exposition of colon cancer cells cultured in BIOMIMESYS® Oncology matrix to an anti-proliferative drug showed a closer in vitro/in vivo correlation in the EC50 curve compared to 2D culture. Cancer cells can be advantageously grown in BIOMIMESYS® for several weeks in multiwell plates and in microfluidic chips for more advanced models. We also observed that modifications in the matrix composition and stiffness modify the cell behavior. Moreover, thanks to collaborations with academic laboratories, we demonstrated that BIOMIMESYS® allows to reproduce in vitro the behavior of cancerous cells in vivo, like mutation effects and metastasis propagation, and could be a relevant alternative to animal models. These results showed that the matricial microenvironment modifies the cell behavior in vitro and should be considered carefully in drug discovery. BIOMIMESYS® hydroscaffold™ is adapted to High Content Screening and represented a powerful tool to better select drug candidate.
paper 2
This study investigated how tumor secreted factors alter the responses of human endothelial cells (HUVECs) to matrix stiffness during capillary formation. The researchers used synthetic hydrogels of varying stiffness to culture HUVECs with or without tumor cells. They found that while HUVECs prefer a substrate of intermediate stiffness for optimal capillary formation in the absence of tumor factors, stimulation by tumor secreted factors disrupts the HUVECs' mechanosensitive behavior. Additionally, the anti-angiogenic drug vandetanib was less effective at reducing capillary formation when HUVECs were activated by tumor factors, particularly on stiffer gels. This suggests tumor activation alters the mechanosensitivity and drug responsiveness
Identification of a new way to induce differentiation of dermal fibroblasts i...
Identification of a new way to induce differentiation of dermal fibroblasts i...Santiago Giraldo Rodas
The document discusses human dermal fibroblasts and vascular endothelial cells. It summarizes that human dermal fibroblasts are abundant in the skin and contribute to connective tissue formation. When damaged, fibroblasts are activated to repair tissues by producing extracellular matrix. The objective is to identify a new way to induce differentiation of dermal fibroblasts into vascular endothelial cells. Methods described include western blot analysis, quantitative real-time PCR, immunofluorescence microscopy, and flow cytometry to analyze protein and gene expression changes during the differentiation process. Results and discussion sections describe findings supporting the roles of VEGF, PDGF, and FGF signaling pathways in endothelial progenitor cell and vascular endothelial cell differentiation. The conclusions note that advances in molecular biology techniques can enable new medical treatments such as using cellTumour targeting and Brain specific drug delivery
The document discusses tumor targeting and brain specific drug delivery. It provides an introduction to targeted drug delivery and outlines strategies for tumor targeting including passive targeting via the enhanced permeability and retention effect, active targeting using ligands, and triggered drug delivery responsive to microenvironment changes. It also discusses challenges of drug delivery to the brain posed by the blood-brain barrier and factors that affect crossing it, as well as diseases related to the brain and strategies to enhance brain-specific drug delivery.
2D & 3D cell cultures
This document discusses 3D cell culture systems and their application in drug discovery. It notes that 3D cell cultures better mimic the in vivo cellular environment compared to traditional 2D cultures. Cells in 3D cultures exhibit different gene expression, morphology, proliferation rates, and responses to drugs compared to 2D cultures. This makes 3D cultures more predictive of in vivo responses during drug testing. The document outlines different types of 3D culture systems, such as scaffold-based, scaffold-free, spheroids and organoids. It also discusses advantages of 3D cultures for applications in areas like developmental biology, disease modeling, regenerative medicine, and personalized drug testing.
Weber-Thesis
This thesis investigated using ellipsometry to analyze ligand binding to G-protein coupled receptors (GPCRs). GPCRs are important cell surface proteins linked to many diseases. Ellipsometry is an optical technique that can quantify ligand-receptor interactions by measuring changes at a surface when polarized light is reflected off. An A549 epithelial cell line expressing the CXCR4 GPCR and its ligand CXCL12 was used. Enzyme-linked immunosorbent assays were performed to determine optimal ligand and antibody concentrations. Baseline characterization of glass slides and binding experiments with ligand and antibody were conducted using ellipsometry. Psi-delta analysis of collected data showed trends in binding.
3D In Vitro Model for Drug Efficiency Testing
This document discusses the potential advantages of using 3D in vitro models compared to traditional 2D models for drug testing. It notes that 3D cultures more closely mimic the in vivo microenvironment and cell morphology. This allows 3D cultures to better predict cellular responses to drugs and provide more accurate models of disease. The document outlines several applications of 3D cultures, such as studying tumor development, evaluating drug sensitivity, and developing organs-on-chips microfluidic devices that model human organ functions.
Amiji poster
1) Researchers are investigating a therapeutic approach for treating multi-drug resistant triple negative breast cancer by manipulating mitochondrial networks.
2) They developed liposomes containing a peptide that breaks up mitochondrial fusion and tested them on cancer cells along with drugs to induce cell death.
3) Preliminary results found the mitochondrial-targeting peptide was effective at breaking up fusion in drug-resistant cancer cells, suggesting this approach may provide a treatment for multi-drug resistant breast cancer.
Protein and peptide delivery system
This slide show is created for the purpose of helping pharma people involved in research in drug delivery methods.
Stem cells and nanotechnology in regenerative medicine and tissue engineering
Stem cells and nanotechnology in regenerative medicine and tissue engineeringDr. Sitansu Sekhar Nanda
Alexis Carrel, winner of the Nobel Prize in Physiology or Medicine in 1912 and the father of whole-organ transplant, was the first to develop a successful technique for end to end arteriovenous anastomosis in transplantation.
Introduction to Oligonucleotides and vaccines
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2) A case study demonstrates that treatment with an antisense oligonucleotide targeting SCN8A increased survival in mouse models of epileptic encephalopathies by inhibiting gene expression.
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Introduction to endocrinology & communication 1st year 2018-19
Physiology of Introduction to endocrinology & communication.
Cell to Cell Communication.
Prof. Dr. Rashid Mahmood
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This document summarizes a study on developing a novel protein interaction platform to study neurodegeneration. The study cultured neuronal stem cells to form neurospheres, which were used as a model system. Lentiviral transfection was optimized to introduce target genes stably into the neurospheres. The goal was to analyze protein interactions of Alzheimer's disease proteins by co-immunoprecipitation of neurosphere cultures expressing tagged target proteins. This model aims to further the understanding of molecular mechanisms in neurodegenerative disorders like Alzheimer's disease.Organoids: A Foremost Hope For The Future
This is my short presentation in one of my university classes. It's obvious that the future of the stem cell biology is tightly engaged with organoids and they will absolutely change the way science is going to.
Kind regards
Shahin Ahmadian
Cell Mechanisms Presentation.pdf
This document provides an overview of a presentation on cell mechanisms that covers four main topics:
1) The mechanisms that confine cells and tissues to specific anatomical sites through intercellular adhesion and memory.
2) Four types of cellular adaptations.
3) A comparison of necrosis and apoptosis, the two main types of cell death. Necrosis is unregulated cell death that causes inflammation, while apoptosis is a regulated process of cell suicide.
4) At least 20 slides are required to be created in PowerPoint or Impress covering these topics using at least four scholarly sources.
Autologous Bone Marrow Cell Therapy for Autism: An Open Label Uncontrolled C...
The aim of this study is to assess the safety and effectiveness of autologous bone marrow
mononuclear stem cell (BMMNC) transplantation in patients with autism.
Alzheimer 'Disease-in-a-Dish' and Development of Novel Therapeutics
Alzheimer 'Disease-in-a-Dish' and Development of Novel TherapeuticsUniversity of Texas at San Antonio
George Perry presented on developing a stem cell model of Alzheimer's disease (AD) as a platform to better understand the human disease and develop drugs. Traditional models like human tissue, animal models, and cell cultures have major limitations. Perry's team aims to generate induced pluripotent stem cells from AD patients to create "disease-in-a-dish" models that exhibit key AD features. Their preliminary studies show AD cells have mitochondrial abnormalities and amyloid-beta overexpression can disrupt mitochondria, supporting the potential of this human stem cell approach.2013-11-26 DTL FIH symposium, Leiden
1) The document discusses the use of protein and metabolite biomarkers in personalized healthcare, noting that over 100 biomarkers are now included in drug labels and 16 companion diagnostics are needed.
2) It describes how companion diagnostics can help determine a drug's metabolism, efficacy, or safety for a patient. Systems biology approaches that integrate multi-omic data are important for developing personalized treatment approaches.
3) The Radboud Center for Proteomics, Glycomics and Metabolomics performs various 'omics analyses including proteomics, glycoproteomics, metabolomics, and top-down proteomics to discover and validate biomarkers for personalized healthcare applications like diagnosing rare diseases, detecting inborn errors of metabolism, and characterizing
Molecular basis of targated drug delivery system chatap
The document discusses targeted drug delivery systems and their molecular basis. It describes various types of targeted delivery such as ligand-driven receptor mediated delivery and active targeting using carriers with ligands. The key components of targeted delivery systems are described, including carriers to protect and deliver drugs to targets. Methods of targeting include passive targeting of tissues as well as active targeting of cells and intracellular sites using ligands that bind to receptors to direct delivery.
Mitochondria in biology and medicine art
1. The document summarizes key findings from the second annual conference of the Society of Mitochondrial Research and Medicine held in India in November 2012.
2. Presentations highlighted the regulatory role of mitochondria in various cellular processes and their involvement in diseases like cancer, neurodegeneration, aging, and inflammation.
3. Evidence was presented linking environmental toxins like cadmium to mitochondrial dysfunction and diseases like diabetes through disrupted signaling pathways and reactive oxygen species production.
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Poster HCSPHARMA (OncoLilleDays2022) - Mechanobiological characterization of ...
Thomas Meynard, PhD student in OncoLille (under the supervision of Vincent Senez and Isabelle Van Seuningen) in collaboration with HCS Pharma too, presented a poster showing that it is possible to include BIOMIMESYS® in a microfluidic chip to co-culture Cancer-Associated fibroblasts and cancerous cells, with the aim to increase the complexity and the relevance of in vitro cancer models.
Poster : Development of personalized therapeutic targeting in lung cancer wit...
Abstract: Lung cancer is one of the most frequent cancers in the world with a high mortality rate. The discovery of oncogenic alterations in these cancers allowed the development of targeted therapies against several receptor tyrosine kinases (RTK) including EGFR, ALK or MET and contributed to improve the prognosis of some patients. Mutations impacting the MET receptor exon 14 splice sites (METex14) have recently been detected in about 3% of lung cancers and lead to the loss of the juxtamembrane domain with several negative regulatory sites. METex14 does not lead to constitutive activation of the receptor but we demonstrated that HGF, its ligand, is required for the full development of the transforming capabilities of the METex14 receptor in SCID-HGFhuman transgenic mice.
Several clinical trials have shown that only half of METex14 patients responded to MET inhibitors. While encouraging, these results are lower than those obtained with other treatments such as EGFR inhibitors. Interestingly, METex14 mutations are frequently associated with other molecular alterations including PTEN loss or TP53 mutations. Furthermore, we have shown that many METex14 mutated patients have autocrine secretion of HGF ligand. Understanding how these co-alterations impact METex14 oncogenicity and sensitivity to existing therapies is of outmost importance to identify patients that could benefit from them.
HCS PHARMA - (ECM 2022) Development of innovative hiPSC-based model including...
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<p>We previously showed that human pluripotent stem cells (hiPSCs) provide a suitable model to study<br>metabolic diseases upon hepatocyte-like cell (HLC) differentiation. With a non-invasive approach, hiPSCs can be generated from urine samples of patients and HLCs have been used to model cholesterol metabolism regulation, by the study of LDLR- and PCSK9-mediated autosomal dominant hypercholesterolemia (ADH) as well as PCSK9-mediated familial hypobetalipoproteinemia (FHBL). This model provides promising advantages with a direct link to the patient and with an unlimited source of HLCs. But like all models, there are limitations, mainly by the neonatal characteristic of HLCs lead to difficulties for pharmacological investigations.</p>
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<p>Therefore, to overcome these burdens, we chose to 1. Differentiate hiPSCs into HLCs in an innovative<br>3D <a href="https://hcs-pharma.com/biomimesys/">hyaluronic acid-based hydroscaffold</a>, BIOMIMESYS® produces by HCS Pharma to enhance their maturation. 2. Adapt our 3D differentiation process to a 96-well format to make it compatible for drug screening. 3. Characterization of the 3D HLCs model by metabolism tests and compare to primary human hepatocyte (PHH).</p>
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<p>We gathered 3’ SRP data all along the differentiation process and RNAseq has been performed by comparing 2D and 3D differentiation conditions to characterize hiPSCs differentiation into liver organoids. We observed an enhanced expression of most hepatic genes and genes expressed by non-parenchymal cells such as stellate cells. Immunofluorescence data confirmed the co-localization of albumin-positive<br>hepatocytes, desmin-positive stellate cells and LYVE1-positive endothelial cells in liver organoids. Finally, at a functional level, several CYP activities including CYP3A4 were detected at the basal level and successfully induced. Liver organoids responded to pharmacological treatments as shown by their ability to accumulate lipids upon amiodarone treatment or uptake LDL-bodipy upon statin treatment.</p>
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<p>Altogether, our development gave rise to functional liver organoids generated with a unique and common procedure, in a process of automating for future high throughput screening.</p>
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HCSPHARMA Importance of microenvironment in cerebral in vitro models for phen...
Aim: About 90% of drug-candidates failed in clinical trials, in particular in neurology, due to a lack of efficacy. That highlights a lack of relevance in preclinical models, including in vitro models, which do not take into account the microenvironment, composed by glial cells and the Extracellular Matrix (ECM). The objective was to study the influence of the microenvironment in cerebral in vitro models, in the frame of Parkinson’s Disease (PD).
Methods: First, we analyzed the influence of astrocytes on Luhmes cell sensitivity, a dopaminergic neuronal cell line, in 2D culture. Then, we developed a hyaluronic acid-based hydroscaffold for 3D cell culture, which mimics the ECM, and study the sensitivity of Luhmes cells in this model. Thirdly, we performed a co-culture of Luhmes cells and astrocytes in this matrix, to form a complex model including both the glial and the matricial microenvironments.
Results: We observed a protective effect of astrocytes in 2D culture. In the hydroscaffold, Luhmes cells displayed a lower sensitivity compared to 2D culture, that was explained by a partial retention of toxic molecules in the matrix, and differences in neuronal protein expression. In the co-culture, we observed spheroids containing both neurons and astrocytes.
Conclusions: This work highlighted that the microenvironment of neurons can modify the neuronal response in vitro, and should thus be considered carefully in academic research and in drug discovery. This model can be now used to study the microenvironment modifications in pathological conditions, and to develop innovative drugs targeting the microenvironment.
BIOMIMESYS®Liver, a 3D cell culture model for maintaining and promoting hepat...
BIOMIMESYS® range are hyaluronan based hydroscaffold developed to overcome the 2D flat culture limitations by recreating an in vivo-like physiology within the in vitro environment.
BIOMIMESYS®Liver hydroscaffold is made of RGDS and galactosamine-grafted Hyaluronic acid, Adipic acid dihydrazide crosslinker and extracellular matrix (ECM) proteins (collagen type I and collagen type IV) to mimic liver ECM composition.
Abstract : a single procedure to generate functional hi ps cs-derived liver o...
Abstract for poster for the "VIIIème Colloque de Génomique Fonctionnelle du Foie" - Rennes (France) 2020
Poster – Development and automation of 3D innovative hiPSC-based liver organo...
We previously showed that human pluripotent stem cells (hiPSCs) provide a suitable model to study metabolic diseases upon hepatocyte-like cell (HLC) differentiation. In particular, HLCs have been used to model cholesterol metabolism regulation, by mimicking the main disease features in vitro. Human iPSCs can be generated from urine samples of patients with a well-described phenotype and carrying specific genotypes. This non-invasive approach allowed the study of LDLR- and PCSK9-mediated autosomal dominant hypercholesterolemia (ADH) as well as PCSK9-mediated familial hypobetalipoproteinemia (FHBL). While the direct link between hiPSCs and patients, as well as the abundance of HLCs provide promising advantages of such strategy, it is impaired mainly by the neonatal characteristic of HLCs as well as the difficulty to perform high throughput studies for pharmacological investigations.
Development of a new liver-on-chip including BIOMIMESYS® technology for mimic...
Objective: to develop a new liver-on-chip model that includes a relevant 3D matrix for hepatic cell growth and function with the use of of BIOMIMESYS® Liver hydroscaffold for a physiological 3D hepatocyte culture.
HCS Pharma complète sa gamme de culture cellulaire en 3D BIOMIMESYS® avec BIO...
Après le succès de la gamme BIOMIMESYS® reproduisant de manière précise et physiologique le microenvironnement du foie, des tissus adipeux et cancéreux, HCS Pharma étend sa gamme avec BIOMIMESYS® Brain, un système exclusif et innovant permettant la culture cellulaire en 3D de cellules neuronales.
HCS Pharma extends its 3D cell culture range BIOMIMESYS® with BIOMIMESYS® Brain
HCS Pharma is expanding its BIOMIMESYS® 3D cell culture product range with BIOMIMESYS® Brain, a system for culturing neuronal cells that reproduces the physiological conditions of the brain's extracellular matrix. BIOMIMESYS® technology facilitates 3D cell culture using a microenvironment based on hyaluronic acid and other components naturally present in tissues. BIOMIMESYS® Brain is available to assist with in vitro testing for the pharmaceutical and cosmetics industries to help reduce animal testing. HCS Pharma also offers developing cell culture protocols for specific cell types using its BIOMIMESYS® technology.
A groundbreaking 3D cell culture technology for HCS: BIOMIMESYS hydroscaffold
Most potential drug candidates (90%) fail within the clinical trials, mainly because of lack of efficacy.
What if the pharmaceutical industry uses predictive human in vitro models in early drug discovery ?
3D innovative hiPSC-based models including the microenvironment for phenotyp...
This document summarizes research from the Thorax Institute on using induced pluripotent stem cells (iPSCs) to model metabolic diseases like familial hypercholesterolemia (FH). Researchers generated iPSCs from a FH patient and differentiated them into hepatocyte-like cells. These cells reproduced the patient's phenotype of impaired LDL cholesterol uptake and responded to pharmacological treatment. The researchers propose that 3D culture systems could further mature the cells to enable phenotypic screening of diseases like non-alcoholic steatohepatitis and fibrosis.
Neurotoxicity assessment: Comparison between SH-SY5Y and iPSC-derived cells
The document compares the sensitivity of iPSC-derived neuronal cells and SH-SY5Y cells to 32 compounds. Two types of iPSC-derived cells are tested: central nervous system cells (CNS.4UTM) and peripheral nervous system cells (PERI.4UTM). The cells were treated with compounds for 48 hours and cell viability and neurite outgrowth were measured. The results showed that CNS.4UTM and PERI.4UTM cells were more sensitive to neurotoxic compounds compared to SH-SY5Y cells. Certain compounds like colchicine, suramin, and nocodazole produced neurotoxic effects in all three cell types.
BIOMIMESYS® Biofunctionalized hydroscaffold for 3D cell culture
To better mimic the tissue microenvironment and provide more
predictive tools for drug development.
hello@biominesys.com
HCS for brain disorders / HCS Pharma at B4B mars 2018
How phenotypic screening with innovative cellular models can help to unravel mechanisms or repurpose drugs in the frame of brain disorders
Présentation de HCS Pharma dans la gazette du laboratoire - 2017
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Urine sample-derived human induced pluripotent stem cells as a model to study...
1. The document describes research from the l’institut du thorax on proprotein convertase subtilisin/kexin type 9 (PCSK9) and its role in cholesterol regulation.
2. Gain-of-function mutations in PCSK9 are associated with hypercholesterolemia, while loss-of-function mutations are linked to lower cholesterol levels and potential cardioprotective effects. This has led to the development of a new class of cholesterol-lowering drugs.
3. The researchers generated induced pluripotent stem cells from patients with PCSK9 gain-of-function and loss-of-function mutations. Differentiating these into hepatocyte-like cells
3D culture in phenotypic screening : advantages, process changes and new tech...
It was a real pleasure to be in the « High-Content and Phenotypic Screening » meeting in Cambridge. We were invited by our partner Molecular Devices to give a talk during the "User meeting Molecular Devices" about our vision of 3D culture in phenotypic screening and the impact of new technologies. We also presented a poster about "Neurotoxicity assay on 2D and 3D culture using High Content Screening technology".
Neurotoxicity assay on 2D and 3D culture using High Content Screening (HCS) t...
As shown by AstraZeneca in nature reviews*, one third of safety failures along the drug discovery process is linked to CNS toxicity uncovered in clinical trials. To avoid this attrition, the potential neurotoxicity of any drug going through the blood brain barrier (BBB) needs to be assessed in the very early stages of new chemical entities (NCE) research. Neurotoxicity assays can be performed on the SH-SY5Y human cell line by using High-Content Screening (HCS) technologies. The present study was performed using classical 2D and 3D culture protocols. In this poster, 2D results and preliminary 3D culture results on multiple reference compounds are depicted.
Augmented and Virtual Reality for the future of laboratories
As you know, immersive technologies change many things in our way to work. Of course, biology and biotechnology companies are also concerned by this trend. In HCS Pharma, we use to explore these fields to improve the quality (and the readability) of our results. We think it's a natural behavior, to keep good relationships with our customers.
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HCS for brain disorders / HCS Pharma at B4B mars 2018
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KEY Points of Leicester travel clinic In London doc.docx
In order to protect visitors' safety and wellbeing, Travel Clinic Leicester offers a wide range of travel-related health treatments, including individualized counseling and vaccines. Our team of medical experts specializes in getting people ready for international travel, with a particular emphasis on vaccines and health consultations to prevent travel-related illnesses. We provide a range of travel-related services, such as health concerns unique to a trip, prevention of malaria, and travel-related medical supplies. Our clinic is dedicated to providing top-notch care, keeping abreast of the most recent recommendations for vaccinations and travel health precautions. The goal of Travel Clinic Leicester is to keep you safe and well-rested no matter what kind of travel you choose—business, pleasure, or adventure.
PrudentRx: A Resource for Patient Education and Engagement
PrudentRx enhances healthcare by educating patients and ensuring effective medication management in a complex, evolving landscape.
How Effective is Homeopathic Medicine for Anxiety and Stress Relief.pdf
Have you ever wondered how effective is homeopathic medicine for anxiety and stress relief? Let’s dive into this fascinating topic.
The Power of Superfoods and Exercise.pdf
Healthy Eating Habits:
Understanding Nutrition Labels: Teaches how to read and interpret food labels, focusing on serving sizes, calorie intake, and nutrients to limit or include.
Tips for Healthy Eating: Offers practical advice such as incorporating a variety of foods, practicing moderation, staying hydrated, and eating mindfully.
Benefits of Regular Exercise:
Physical Benefits: Discusses how exercise aids in weight management, muscle and bone health, cardiovascular health, and flexibility.
Mental Benefits: Explains the psychological advantages, including stress reduction, improved mood, and better sleep.
Tips for Staying Active:
Encourages consistency, variety in exercises, setting realistic goals, and finding enjoyable activities to maintain motivation.
Maintaining a Balanced Lifestyle:
Integrating Nutrition and Exercise: Suggests meal planning and incorporating physical activity into daily routines.
Monitoring Progress: Recommends tracking food intake and exercise, regular health check-ups, and provides tips for achieving balance, such as getting sufficient sleep, managing stress, and staying socially active.
RECENT ADVANCES IN BREAST CANCER RADIOTHERAPY
ULTRA HYPOFRACTIONATION
Accelerated Partial Breast Irradiation: :Brachytherapy
UK FAST FORWARD
Gemma Wean- Nutritional solution for Artemia
GEMMA Wean is a high end larval co-feeding and weaning diet aimed at Artemia optimisation and is fortified with a high level of proteins and phospholipids. GEMMA Wean provides the early weaned juveniles with dedicated fish nutrition and is an ideal follow on from GEMMA Micro or Artemia.
GEMMA Wean has an optimised nutritional balance and physical quality so that it flows more freely and spreads readily on the water surface. The balance of phospholipid classes to- gether with the production technology based on a low temperature extrusion process improve the physical aspect of the pellets while still retaining the high phospholipid content.
GEMMA Wean is available in 0.1mm, 0.2mm and 0.3mm. There is also a 0.5mm micro-pellet, GEMMA Wean Diamond, which covers the early nursery stage from post-weaning to pre-growing.
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TEST BANK For Accounting Information Systems, 3rd Edition by Vernon Richardson, Verified Chapters 1 - 18, Complete Newest Version
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TEST BANK For Accounting Information Systems, 3rd Edition by Vernon Richardson, Verified Chapters 1 - 18, Complete Newest VersionTop massage center in ajman chandrima Spa
We are one of the top Massage Spa Ajman Our highly skilled, experienced, and certified massage therapists from different corners of the world are committed to serving you with a soothing and relaxing experience. Luxuriate yourself at our spas in Sharjah and Ajman, which are indeed enriched with an ambiance of relaxation and tranquility. We could confidently claim that we are one of the most affordable Spa Ajman and Sharjah as well, where you can book the massage session of your choice for just 99 AED at any time as we are open 24 hours a day, 7 days a week.
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Pneumothorax and role of Physiotherapy in it.
This particular slides consist of- what is Pneumothorax,what are it's causes and it's effect on body, risk factors, symptoms,complications, diagnosis and role of physiotherapy in it.
This slide is very helpful for physiotherapy students and also for other medical and healthcare students.
Here is a summary of Pneumothorax:
Pneumothorax, also known as a collapsed lung, is a condition that occurs when air leaks into the space between the lung and chest wall. This air buildup puts pressure on the lung, preventing it from expanding fully when you breathe. A pneumothorax can cause a complete or partial collapse of the lung.
Innovative Minds France's Most Impactful Healthcare Leaders.pdf
This edition features a handful of Innovative Minds: France's Most Impactful Healthcare Leaders that are leading us into a better future.
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Feeding plate for a newborn with Cleft Palate.pptx
A feeding plate is a prosthetic device used for newborns with a cleft palate to assist in feeding and improve nutrition intake. From a prosthodontic perspective, this plate acts as a barrier between the oral and nasal cavities, facilitating effective sucking and swallowing by providing a more normal anatomical structure. It helps to prevent milk from entering the nasal passage, thereby reducing the risk of aspiration and enhancing the infant's ability to feed efficiently. The feeding plate also aids in the development of the oral muscles and can contribute to better growth and weight gain. Its custom fabrication and proper fitting by a prosthodontist are crucial for ensuring comfort and functionality, as well as for minimizing potential complications. Early intervention with a feeding plate can significantly improve the quality of life for both the infant and the parents.
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Importance of matricial and cellular microenvironments in in vitro models for drug discovery in Parkinson’s disease
- 1. Introduction What is the extracellular matrix (ECM)? Conclusion and perspectives Importance of matricial and cellular microenvironments in in vitro models for drug discovery in Parkinson’s disease Results What is BIOMIMESYS® hydroscaffold? Hyaluronic acid grafted with other ECM components : Composition and physico-chemical properties tailored to mimic the ECM of each organ. BIOMIMESYS® Brain : HA, RGDS, Collagen IV, cationic polymer. Elastic modulus 0.1 kPa. References The ECM has diverse and crucial roles, in health and disease For more information, go to : hcs-pharma.com or scan this QR-code. * : veronique.deconto@hcs-pharma.com De Conto V. (Loos, France)1*, Cheung V. (Loos, France)1, Maubon G. (Loos, France)1, Souguir Z. (Loos, France)1, Maubon N. (Loos, France)1, Vandenhaute E. (Loos, France)1, Bérézowski V. (Lille, France)2 1HCS Pharma, Loos, France ; 2Lille Neuroscience & Cognition, Univ. Lille, Inserm, CHU-Lille, Lille, France • Low success rate in drug discovery : 90% of failure in clinical trials (safety and efficacy) • The Central Nervous System is highly involved : The presence of ECM and glial cells influenced the sensitivity of dopaminergic neurons to PD inducers. We now aim at testing PD inducers on the co-culture in 3D. This model represents a promising tool for the study of complex and chronic diseases such as PD. Goal : To improve the predictivity of in vitro models • Taking into account the 3D cell organization • Including the cellular and matricial microenvironments Lack of relevance of preclinical models to select drug candidates Cook et al. 2014 Brain ECM: • 20% of cerebral volume • Ratio GAG*/Collagen : 10/1 Low stifness Hydroscaffold Hydrogels: Biohydric medium Cells encapsulated Pathological or no relevant ECM Solid Scaffolds: Porous, No ECM Decellularized organs Complete ECM Difficult tissue access, not reproducible Porous, physiological, reproducible. • Impact of matrix on neuronal sensitivity • Co-culture of neuronal and glial cells • Impact of astrocytes on neuronal sensitivity (2D) Methods The cells were less sensitive to PD* inducers in 3D that in 2D, that was partially or totally explained by molecules retention in the matrix. The PD inducers led to the activation of astrocytes (increase in GFAP expression). However, the presence of astrocytes decreased the cell sensitivity, probably link to their secretion properties which can promote cell adhsesion or protect cells. *GAG: Glycosaminoglycans *PD: Parkinson’s disease Cook, D., Brown, D., Alexander, R., March, R., Morgan, P., Satterthwaite, G., Pangalos, M.N., 2014. Lessons learned from the fate of AstraZeneca’s drug pipeline: a five-dimensional framework. Nat. Rev. Drug Discov. 13, 419–431. https://doi.org/10.1038/nrd4309 Illustration source : Biorender Luhmes + erastin Coculture + erastin Luhmes Coculture Scale bar = 200µm. (Scanning electron microscope) GFAP TUBB3