How to make in vitro models predictive of in vivo conditions? - By taking into account the 3D cellular organization of in vivo tissues - By including the cellular and matricial microenvironments with BIOMIMESYS® - By using OoC systems for dynamic in vivo-like in vitro systems Dynamic models hold promise for future predictive microphysiological systems (MPS). By combining BIOMIMESYS® as an ECM surrogate for 3D culture, and hiPSC-derived cells, these dynamic microfluidic systems will revolutionize the field, reproducing human tissues and predict human outcomes.

1. BIOMIMESYS® 3D hydroscaffold: a matricial microenvironment
for physiological organs-on-chip (OoC)
BIOMIMESYS® = hyaluronic acid, grafted with native ECM components.
Its composition and stiffness are tailored to mimic the extracellular matrix (ECM) of each
tissue/organ of interest.
For more information, go to www.hcs-pharma.com
Scan the QR-code for a digital version of this poster
* : meryl.roudaut@hcs-pharma.com
M. Roudaut1*, E. Vandenhaute1, Z. Souguir1, N. Maubon1
1HCS Pharma, Loos, France
Dynamic models hold promise for future predictive
microphysiological systems (MPS). By combining BIOMIMESYS®
as an ECM surrogate for 3D culture, and hiPSC-derived cells,
these dynamic microfluidic systems will revolutionize the field,
reproducing human tissues and predict human outcomes.
How to make in vitro models predictive
of in vivo conditions?
• By taking into account the 3D cellular
organization of in vivo tissues
• By including the cellular and matricial
microenvironments with
• By using OoC systems for dynamic in
vivo-like in vitro systems
Hydroscaffold
Hydrogels:
Biohydric medium
Cells encapsulated
Pathological, or
irrelevant ECM
Solid Scaffolds:
Porous
Irrelevant extracellular
matrix (ECM)
Decellularized tissue
Complete ECM
Difficult tissue access
Not reproducible
Scanning electron microscopy pictures
Towards hiPSC-derived liver organoids (static) Functional tests in liver organoids (static)
DAPI
2-Deoxy-D-glucose
Merge
Untreated
Insulin 6nM
50µm
50µm
50µm
50µm
50µm
50µm
DAPI LDL-bodipy Merge
Untreated
Mevastatin
50nM
50µm 50µm 50µm
50µm 50µm 50µm
Liver organoid
Albumin
Desmin
DAPI
Merge
Amiodarone or ethanol-
induced lipid accumulation
Mevastatin-induced LDL-bodipy
internalization
Insulin-induced 2DG
internalization
50µm 50µm 50µm
DAPI Nile Red Merge
Untreated
50µm 50µm 50µm
Amiodarone
20µM
50µm 50µm 50µm
Ethanol
200nM
Immunofluorescence characterization
Merge
DAPI ZO-1 Merge
50µm 50µm 50µm
50µm 50µm 50µm 50µm
DAPI CD31 LHX2 Merge
50µm 50µm 50µm 50µm
DAPI OATP1B1 LYVE1 Merge
in
biochips
Introduction What is BIOMIMESYS® 3D hydroscaffold?
BIOMIMESYS® hydroscaffold in-a-chip
Avantages of BIOMIMESYS®
• Can be cast within biochips
• Organ-specific porous matrix
• Resists to the flow along time,
compared to hydrogel systems
• Easy and ready-to-use in your
biochips!
Micro3DBeta PhD project (2020-2023)
Developing a 3D dynamic microfluidic device of pancreatic beta cells and
adipocytes to model multi-organ crosstalks during type 2 diabetes
development
MIMLiverOnChip project (2018-2023)
Developing a functional liver-on-chip system to evaluate the toxicity of drugs
• Universal “plug-and-play” liver-on-chip platform
• Mimicking the major events occurring in the liver sinusoid
is:
 Porous
 Physiological
 Reproducible
MCF-7
Hoechst
Calcein-AM
Propidium iodide
MDA-MB-231
200 um
96-well plate, static culture
7 days 7 days 28 days
ONCO3D project (2017-2022)
Developing tumoral models for modeling cancer development and responses to drug
Ibidi biochip, dynamic culture
Primary human
adipocytes in BioPharMEMs Common Laboratory (2020-2022)
Designing and industrializing new pharmacological in vitro assays for the screening of
compounds/biopharmaceuticals in 3D, combining microfluidics, BioMEMS systems and ECM
Development of a 3D in vitro microfluidic culture system to study
tumor-stroma interactions and drug resistance in pancreatic
adenocarcinoma or PDAC (ongoing)
• 3D microfluidic bioreactor as a PDAC model for drug
testing
• Microenvironment made of BIOMIMESYS and
pancreatic stellate cells
• Implementation of interstitial fluid flow and
nutrient/oxygen gradients
• Growth of 3D cells aggregates
• Study of mechanical effects on tumor phenotype
Conclusion and perspectives
Want to know more?
Adipose tissue Pancreatic beta
cells
(Use of smart.servier.com for this illustration)
Louis et al., 2017
Pr Cecile Legallais Dr Anthony Treizebre
Dr Damien Fleury
Dr Fabrice Soncin
Pr Isabelle Van Seuningen
Dr Vincent Senez
Dr Jean-Sebastien Annicotte
Dr Anthony Treizebre