BIOMIMESYS is an innovative hydrogel scaffold produced by HCS Pharma for 3D cell culture. It is made of hyaluronic acid and collagen, which mimic the extracellular matrix. Cells cultured in BIOMIMESYS form structures that closely resemble in vivo conditions. This allows cells to be studied under more physiologically relevant conditions for applications in drug development, metabolic studies, and cancer research. BIOMIMESYS is an easy to use, ready-to-use product suitable for various downstream applications and analysis techniques. It has been successfully used with many cancer cell lines.

1. HCS Pharma – Siège de Lille
Biocentre Fleming Bat A
250 rue Salvador Allende
59120 LOOS
BIOMIMESYS®
is a registered trademark
of HCS Pharma
+33 769 999 137
www.biomimesys.com
hello@biomimesys.com
Innovative Hydroscaffolds™ for 3D Cell Culture to better mimic the tissue microenvironment and
provide more predictive tools for drug development and metabolic studies
Other BIOMIMESYS® products available
PRODUCT
BIOMIMESYS® Oncology is a new generation of
mimetic Hydroscaffolds™ for 3D cell culture. Available in
a ready-to-use format, it enables the culture of cells
under physiological conditions that are representative of
the microenvironment found in whole tissues. The highly
porous structure of BIOMIMESYS® allows cells to diffuse
into the 3D matrix, where they can fix and begin to
develop. The structure and conformation of cells cultured
in BIOMIMESYS®, such as cancer cells that
spontaneously form spheroids, strongly resemble the
structures formed in vivo. Also, the functionality of the
BIOMIMESYS® matrix is proved by the expression of
genes and proteins at levels that are again more similar
to those found in vivo. Cells cultured in BIOMIMESYS® give results that are more physiological than
those grown in 2D culture and are closer to those obtained in vivo.
Figure 1: SEM picture

2. HCS Pharma – Siège de Lille
Biocentre Fleming Bat A
250 rue Salvador Allende
59120 LOOS
BIOMIMESYS®
is a registered trademark
of HCS Pharma
+33 769 999 137
www.biomimesys.com
hello@biomimesys.com
Hyaluronic acid (HA) being a major component of the cell’s extracellular matrix (ECM), BIOMIMESYS®
Hydroscaffolds™ for 3D cell culture is an ideal study model because it is:
 Highly porous for the simple extraction of RNA and proteins
 Biodegradable for easy cell retrieval
 Transparent for direct visualization of cells
 Representative of the cell microenvironment in terms of cell-cell interactions, cell-matrix
interactions and the expression of proteins and genes
 BIOMIMESYS® Oncology is made of HYALURONIC ACID (HA), a glycosaminoglycan. Hyaluronic acid is a major
component of the extracellular matrix (ECM), and it plays an IMPORTANT ROLE in connective tissue.
 The HA-based Hydroscaffolds™ are formed by crosslinking HA and type I collagen with ADH to form
reticulated chains.
 High molecular weight Hyaluronic acid + type I collagen.
 BIODEGRADABLE product.
 PHYSICOCHEMICAL FEATURES
o Porosity: 150-200um
o Rheology: Young’s modulus: E= 1 kPa +/-Swelling ratio = 75 ± 10g/g
READY-TO-USE
BIOMIMESYS®
Hydroscaffolds™ technology is EASY to handle handling & READY-TO-USE. Upon receiving
the vacuum sealed 96-well plate open it (under a hood) and add the cells directly on top of the matrix.
Changing the culture medium is easy as well. To remove medium, simply draw the medium with a
pipette between the matrix and the edge of the well. To refresh the medium, place fresh medium onto
the surface of the matrix.
96-well plate format
10-40µl of cell
suspension
dropped on the
surface of
BIOMIMESYS®
Culture medium
is gently added
on the side of
the well to
obtain qs. 200µl

3. HCS Pharma – Siège de Lille
Biocentre Fleming Bat A
250 rue Salvador Allende
59120 LOOS
BIOMIMESYS®
is a registered trademark
of HCS Pharma
+33 769 999 137
www.biomimesys.com
hello@biomimesys.com
COMPATIBLE TECHNOLOGIES
BIOMIMESYS® Hydroscaffolds™ has many properties (translucent, porous, and biodegradable) that
make it ideal for use with numerous downstream applications. The growing cells are easily retrieved
from the matrix by a gentle and rapid procedure, this means that cells cultured in BIOMIMESYS® may
be analyzed using all technologies as shown below.
TRANSPARENT POROUS BIODEGRADABLE SOLID
Microscopy PCRs Flow cytometry Histology
Plate Reader (OD,
fluorescence,
luminescence
Western-Blot
ELISA
SUCESSFULLY TESTED CELLS
Cancer Cells Lines & Primary Tumors
Human brain metastasis SA87
Human breast adenocarcinoma MCF-7
Human breast carcinoma CAL-51
Human cervix adenocarcinoma HeLa
Human colorectal adenocarcinoma DLD-1, HT29, Caco-2
Human glioblastoma CB109 / CB74 / CB191
Human liver hepatocellular carcinoma HepG2
Human liver hepatoma PLC / PRF-5
Human lung carcinoma NCI-H460
Human osteosarcoma SaOs
Human ovarian carcinoma IGROV-1
Human pancreas carcinoma PANC-1
Human prostate cancer PC3
RELATED PUBLICATIONS
 “DIRECT EFFECT OF BEVACIZUMAB ON GLIOBLASTOMA CELL LINES IN VITRO“. SIMON T., COQUEREL B., ET
AL. (2014) NEUROMOLECULAR MED.; 16(4):752-71
 “SURVIVAL OF CORD BLOOD HAEMATOPOIETIC STEM CELLS IN A HYALURONAN HYDROGEL FOR EX VIVO
BIOMIMICRY”. DEMANGE E., KASSIM Y., ET AL. (2012) J TISSUE ENG REGEN MED 7(11):901-10
 “ELASTIN-DERIVED PEPTIDES: MATRIKINES CRITICAL FOR GLIOBLASTOMA CELL AGGRESSIVENESS IN A 3-D
SYSTEM”. COQUEREL B., POYER F., ET AL. (2009). GLIA 57(16): 1716-26.
 “COLLAGENS, STROMAL CELL-DERIVED FACTOR-1ALPHA AND BASIC FIBROBLAST GROWTH FACTOR
INCREASE CANCER CELL INVASIVENESS IN A HYALURONAN HYDROGEL”. DAVID L., DULONG V., ET AL. (2008).
CELL PROLIF 41(2): 348-64

4. HCS Pharma – Siège de Lille
Biocentre Fleming Bat A
250 rue Salvador Allende
59120 LOOS
BIOMIMESYS®
is a registered trademark
of HCS Pharma
+33 769 999 137
www.biomimesys.com
hello@biomimesys.com
 “HYALURONAN HYDROGEL: AN APPROPRIATE THREE-DIMENSIONAL MODEL FOR EVALUATION OF
ANTICANCER DRUG SENSITIVITY.” DAVID L., DULONG V., ET AL. (2008). ACTA BIOMATER 4(2): 256-63
 “RETICULATED HYALURONAN HYDROGELS: A MODEL FOR EXAMINING CANCER CELL INVASION IN 3D.”
DAVID L., DULONG V., ET AL. (2004). MATRIX BIOL 23(3): 183-93
PRICING
BIOMIMESYS® hydroscaffold is sold for RESEARCH USE ONLY.
96-WELL PLATE
**price per plate**
Quantity of 96-well plates 1 ≥ 10 ≥ 100 ≥ 1000
BIO_ONC_96_96_transp
(96 well plate with 96 Hydroscaffolds™)
BIO_ONC_96_24_transp
(96 well plate with 24 Hydroscaffolds™)
Quantity of 96-well *BLACK* plates 1 ≥ 10 ≥ 100 ≥ 1000
BIO_ONC_96_96_black
(96 well plate with 96 Hydroscaffolds™)
BIO_ONC_96_24_black
(96 well plate with 24 Hydroscaffolds™)
BIO_ONC_384_384_black
(384 well plate with 384 Hydroscaffolds™)
Contact Information
HCS Pharma
hello@biomimesys.com
http://www.biomimesys.com