This document discusses bioaerosols and factors related to the transmission of coronaviruses. It summarizes research on the size and properties of airborne viruses, how they bind to carriers in aerosols, and the impact of environmental conditions on viral survival. The document reviews studies showing that viruses like influenza, SARS-CoV-2, and others can remain infectious in aerosols for hours or days, with their survival affected by temperature, humidity, and other physicochemical properties. Understanding these transmission mechanisms could help public health institutions improve prevention strategies for pandemics.
This document summarizes research on the physicochemical properties of coronaviruses and how they affect airborne transmissibility. It discusses how characteristics like virus size, electrical features, and envelope composition influence diffusion properties and a virus's ability to evade the immune system. The authors investigate these physicochemical aspects of coronaviruses and hypothesize that airborne transmission may help explain the rapid spread of COVID-19 beyond direct contact and droplets. Figures and literature are cited to support the role of aerosols in transmitting viruses like coronaviruses.
The document discusses the emergence of new COVID-19 variants in late 2020 and early 2021, including variants first identified in the UK, South Africa, and Brazil. It hypothesizes that air pollution may have contributed to the genetic evolution of these new variants by acting as a mutagenic agent on the virus genome. Specifically, it suggests that airborne pollutants present in the microenvironment of areas with high air pollution, like Wuhan and other industrial regions, could accelerate the natural mutation rate of RNA viruses. The document reviews literature on air pollutants known to be mutagenic and proposes experimental studies to further investigate the potential relationship between air pollution exposure and the emergence of new SARS-CoV-2 variants.
This document provides a detailed review of the outbreak of the coronavirus. It discusses the virus's genome structure, life cycle, clinical features, diagnosis, complications/fatality rates, and preventive measures. The key points are:
- Coronavirus is a large family of RNA viruses that originated in China and has become a global pandemic.
- It has a wide range of clinical presentations, from asymptomatic to multi-organ failure. Common symptoms include fever, cough, and fatigue.
- Diagnosis is made through PCR testing of respiratory samples. Complications can include pneumonia, ARDS, sepsis, and multi-organ dysfunction.
- Preventive measures focus on infection control, isolation, hand washing, and
Identifying Likely Invasion Sites of Zika Virus Infected Mosquitoes through A...QUESTJOURNAL
This document analyzes the potential invasion of Zika virus into Mumbai, India through air and sea ports by infected mosquitoes. The BioTEMS model was used to identify likely invasion sites within 150 km2 of ports and a broader 634 km2 area at risk. Surveillance is recommended at ports and throughout the city. Controlling mosquito breeding in ports, especially fire buckets, could reduce spread. Integrated mosquito management including surveillance and pesticide application may help prevent local transmission if Zika is introduced.
Mucormycosis, also known as black fungus, is a rare fungal infection affecting COVID-19 patients in India. The infection is caused by a group of molds called mucormycetes that live in soil and decaying organic matter. COVID-19 patients have an increased risk due to their weakened immune systems from the virus and immunosuppressive treatments. India has reported over 11,000 cases of black fungus, with the highest numbers in Gujarat, Maharashtra, Andhra Pradesh, Madhya Pradesh, and Telangana. Symptoms include nasal discharge, black lesions, eye and facial swelling. Treatment involves urgent surgery to remove infected tissue combined with antifungal drugs, but mortality
Hundred samples viz. urine, blood, wound, pus and sputum collected from different patients were found to harbour Pseudomonas aeruginosa (P. aeruginosa) (27%) with a maximum isolation from wound samples (33.33%) and minimum from blood samples (11.11%). The degree of resistance of P. aeruginosa isolates to different antibiotics like Ceftazidime (30µg), Amikacin (30µg), Imipenem (10µg), Ciprofloxacin (30µg), Tetracycline (30µg), Gentamicin (10µg), Norfloxacin (10µg), Penicillin (30µg), Chloramphenicol (30µg), and Ofloxacin (5µg) varied from 56% to 100%. Antiseptics i.e. Betadine and Dettol were found to be more effective against the MDR strain of P. aeruginosa at the dilutions of 10-1 and 10-2. Duration of the disease and hospitalization duration, evaluated as risk factors for P. aeruginosa colonization were found to be statistically significant while age and gender were found to be statistically non- significant. The incidence of multidrug resistance of P. aeruginosa is increasing fast due to the frequent use of antibiotics and antiseptics, which are used extensively in hospitals and healthcare centers, therefore it is a need to develop alternative antimicrobial agents for the treatment of infectious diseases.
Key-words- Antibiotic, Antiseptic, Betadine and Dettol, Disinfectants, P. aeruginosa
Human Coronaviruses (HCoV) exhibit positive single stranded RNA genome with enveloped nucleocapsid. Coronavirus belongs to the family Coronaviridae, originated from avian and mammalian species causes upper respiratory tract infection in humans by novel HCoVs viruses named as HCoV-HKU1, HCoV-NL63 but predominant species is Middle East respiratory syndrome (MERS-CoV) across the world. HCoV-HKU1 sp. is associated with chronic pulmonary disease, while HCoV-NL63 causes upper and lower respiratory tract disease in both children and adults, but most recent one was MERS-CoV, which caused acute pneumonia and occasional renal failure. The novel coronavirus SARS-CoV-2 is a new strain that causes the Coronavirus Disease 2019 (COVID-19) as named by the World Health Organization. According to the recent world statistics report about the COVID-19 cases approx. 101,500 confirmed cases and 3,500 death cases appeared. And mostly, a case of infection with CoV was identified in Wuhan, China. Structurally viral genome constitutes of 2/3rd of replicase gene encoding ORFs regions and rest of the 1/3rd region of genome form the structural proteins. The aim of the study was to understand the viral genetic systems in order to facilitate the genetic manipulation of the viral genome and to know the fundamental mechanism during the viral replication, facilitating the development of antidotes against the virus.
This document summarizes research on the physicochemical properties of coronaviruses and how they affect airborne transmissibility. It discusses how characteristics like virus size, electrical features, and envelope composition influence diffusion properties and a virus's ability to evade the immune system. The authors investigate these physicochemical aspects of coronaviruses and hypothesize that airborne transmission may help explain the rapid spread of COVID-19 beyond direct contact and droplets. Figures and literature are cited to support the role of aerosols in transmitting viruses like coronaviruses.
The document discusses the emergence of new COVID-19 variants in late 2020 and early 2021, including variants first identified in the UK, South Africa, and Brazil. It hypothesizes that air pollution may have contributed to the genetic evolution of these new variants by acting as a mutagenic agent on the virus genome. Specifically, it suggests that airborne pollutants present in the microenvironment of areas with high air pollution, like Wuhan and other industrial regions, could accelerate the natural mutation rate of RNA viruses. The document reviews literature on air pollutants known to be mutagenic and proposes experimental studies to further investigate the potential relationship between air pollution exposure and the emergence of new SARS-CoV-2 variants.
This document provides a detailed review of the outbreak of the coronavirus. It discusses the virus's genome structure, life cycle, clinical features, diagnosis, complications/fatality rates, and preventive measures. The key points are:
- Coronavirus is a large family of RNA viruses that originated in China and has become a global pandemic.
- It has a wide range of clinical presentations, from asymptomatic to multi-organ failure. Common symptoms include fever, cough, and fatigue.
- Diagnosis is made through PCR testing of respiratory samples. Complications can include pneumonia, ARDS, sepsis, and multi-organ dysfunction.
- Preventive measures focus on infection control, isolation, hand washing, and
Identifying Likely Invasion Sites of Zika Virus Infected Mosquitoes through A...QUESTJOURNAL
This document analyzes the potential invasion of Zika virus into Mumbai, India through air and sea ports by infected mosquitoes. The BioTEMS model was used to identify likely invasion sites within 150 km2 of ports and a broader 634 km2 area at risk. Surveillance is recommended at ports and throughout the city. Controlling mosquito breeding in ports, especially fire buckets, could reduce spread. Integrated mosquito management including surveillance and pesticide application may help prevent local transmission if Zika is introduced.
Mucormycosis, also known as black fungus, is a rare fungal infection affecting COVID-19 patients in India. The infection is caused by a group of molds called mucormycetes that live in soil and decaying organic matter. COVID-19 patients have an increased risk due to their weakened immune systems from the virus and immunosuppressive treatments. India has reported over 11,000 cases of black fungus, with the highest numbers in Gujarat, Maharashtra, Andhra Pradesh, Madhya Pradesh, and Telangana. Symptoms include nasal discharge, black lesions, eye and facial swelling. Treatment involves urgent surgery to remove infected tissue combined with antifungal drugs, but mortality
Hundred samples viz. urine, blood, wound, pus and sputum collected from different patients were found to harbour Pseudomonas aeruginosa (P. aeruginosa) (27%) with a maximum isolation from wound samples (33.33%) and minimum from blood samples (11.11%). The degree of resistance of P. aeruginosa isolates to different antibiotics like Ceftazidime (30µg), Amikacin (30µg), Imipenem (10µg), Ciprofloxacin (30µg), Tetracycline (30µg), Gentamicin (10µg), Norfloxacin (10µg), Penicillin (30µg), Chloramphenicol (30µg), and Ofloxacin (5µg) varied from 56% to 100%. Antiseptics i.e. Betadine and Dettol were found to be more effective against the MDR strain of P. aeruginosa at the dilutions of 10-1 and 10-2. Duration of the disease and hospitalization duration, evaluated as risk factors for P. aeruginosa colonization were found to be statistically significant while age and gender were found to be statistically non- significant. The incidence of multidrug resistance of P. aeruginosa is increasing fast due to the frequent use of antibiotics and antiseptics, which are used extensively in hospitals and healthcare centers, therefore it is a need to develop alternative antimicrobial agents for the treatment of infectious diseases.
Key-words- Antibiotic, Antiseptic, Betadine and Dettol, Disinfectants, P. aeruginosa
Human Coronaviruses (HCoV) exhibit positive single stranded RNA genome with enveloped nucleocapsid. Coronavirus belongs to the family Coronaviridae, originated from avian and mammalian species causes upper respiratory tract infection in humans by novel HCoVs viruses named as HCoV-HKU1, HCoV-NL63 but predominant species is Middle East respiratory syndrome (MERS-CoV) across the world. HCoV-HKU1 sp. is associated with chronic pulmonary disease, while HCoV-NL63 causes upper and lower respiratory tract disease in both children and adults, but most recent one was MERS-CoV, which caused acute pneumonia and occasional renal failure. The novel coronavirus SARS-CoV-2 is a new strain that causes the Coronavirus Disease 2019 (COVID-19) as named by the World Health Organization. According to the recent world statistics report about the COVID-19 cases approx. 101,500 confirmed cases and 3,500 death cases appeared. And mostly, a case of infection with CoV was identified in Wuhan, China. Structurally viral genome constitutes of 2/3rd of replicase gene encoding ORFs regions and rest of the 1/3rd region of genome form the structural proteins. The aim of the study was to understand the viral genetic systems in order to facilitate the genetic manipulation of the viral genome and to know the fundamental mechanism during the viral replication, facilitating the development of antidotes against the virus.
OMICS Group is an organization committed to making scientific research openly accessible. It hosts over 400 peer-reviewed open access journals and organizes over 300 international conferences annually. The organization has over 3 million readers for its journals. It aims to serve the scientific community by ensuring a rapid peer review process from its large editorial board of over 30,000 reviewers. OMICS Group also partners with over 1000 international societies to make healthcare information openly accessible. It welcomes high-quality research submissions and follows a peer review process to maintain journal excellence.
Structural Design on Virus and its Diversityijtsrd
The coronavirus disease 19 COVID 19 is a highly transmittable and pathogenic viral infection caused by severe acute respiratory syndrome coronavirus 2 SARS CoV 2 , which emerged in Wuhan, China and spread around the world. Genomic analysis revealed that SARS CoV 2 is phylogenetically related to severe acute respiratory syndrome like SARS like bat viruses, therefore bats could be the possible primary reservoir. The intermediate source of origin and transfer to humans is not known, however, the rapid human to human transfer has been confirmed widely. In this document we will analyze the structure and diversity of the pathogen and we will also discuss the previous emergence of human coronaviruses like Severe Acute Respiratory Syndrome Coronavirus SARS CoV and Middle East Respiratory Syndrome MERS CoV . Nadia Naseer "Structural Design on Virus and its Diversity" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-4 | Issue-4 , June 2020, URL: https://www.ijtsrd.com/papers/ijtsrd31225.pdf Paper Url :https://www.ijtsrd.com/biological-science/microbiology/31225/structural-design-on-virus-and-its-diversity/nadia-naseer
1) The document discusses the link between air pollution and the spread and severity of COVID-19. It analyzes literature showing that air pollutants like particulate matter can act as carriers for viral particles and other toxins, worsening the inflammatory response in lungs.
2) Areas with high air pollution were hit harder by the first wave of COVID-19 and the emergence of variants may be related to factors in polluted air acting as mutagens.
3) Reducing global air pollution is needed to curb the effects of COVID-19. The rapid spread of the virus, especially variants, suggests it can be airborne, with implications for prevention measures and international responsibility in addressing pollution.
The document discusses the relationship between COVID-19 vaccines that target the full-length spike protein versus just the receptor-binding domain (RBD), and their potential relationship to procoagulant effects and rare blood clotting issues. Some key points:
- Vaccines like AstraZeneca use the full-length spike protein in viral vectors, while others just target the RBD region.
- The spike protein plays a role in viral entry and interacts with host cell receptors like ACE2. It also has glycans that help disguise the virus.
- Studies found the AstraZeneca vaccine induces cell spikes similar to SARS-CoV-2, eliciting an immune response.
-
This document summarizes a book on the epidemiology and diffusion of viruses with a focus on the role of latitude, air pollutants, and humidity. It discusses several viruses including SARS, MERS, influenza, and COVID-19. It reviews literature finding associations between increased air pollutants like PM2.5 and higher risk of influenza-like illness. Some studies found temperature could impact COVID-19 transmission, with an optimal temperature range. Experiments with influenza in guinea pigs found that cold, dry conditions favor airborne transmission. The role of atmospheric conditions in the seasonality and spread of influenza over large geographic areas is discussed.
This is my first ever Presentation on Coronavirus Pandemic. It is made to grab attention of youngsters,school going children who might be unaware about some important facts of Corona Virus I hope this would be helpful to increase their current affairs.
This document summarizes research on the immunological and toxicological implications of COVID-19, focusing on the innate immune response and immune evasion. It discusses how the virus can trigger a "cytokine storm" through overactivation of the innate immune system and proinflammatory cytokines like IL-6. This storm can lead to widespread inflammation and multi-organ failure. The document also explores potential therapeutic strategies aimed at modulating the cytokine response, such as using corticosteroids or chloroquine to reduce IL-6 levels and calm the storm. Understanding the immune dysregulation and identifying key signaling pathways may help develop new clinical management approaches and prevent progression to severe illness.
This document discusses the field of aeromicrobiology. It defines aeromicrobiology as the study of microorganisms and their spores that are suspended in air but invisible to the naked eye. The document traces the history and evolving definition of aeromicrobiology from the 1930s to today. It also outlines different bioaerosol forms, habitats of microorganisms in the air, and factors that affect their growth. Indoor aeromicrobiology and mechanisms for controlling bioaerosols in indoor environments like laboratories are also summarized.
Activity Relatedness of Environment and Distribution of Air borne Biocontamin...Premier Publishers
Airborne indoor and outdoor bacteria and fungi were assessed during the spring season using conventional methods to investigate the enumeration and identification of airborne micro-organisms taking into consideration anthropogenic variation of the environment. This was determined through air quality sampling using microbial air sampler (M.A.Q.S.II-90, OXOID, UK). The air samples were collected during the onset of harmattan in Nov 2017. four different zones located in Owerri local Govt, were chosen for the collection of airborne bacteria and fungi. These zones were Owerri downtown (Zone A), Imo State University (Zone B), Naze building material layout (Zone C) and the Open unoccupied area along Obinze Port Harcourt road (Zone D). were selected for air bio-pollutant measurement. Cultivation and total microscopic enumeration methods were employed for the sample analysis. Identification of isolates was done using conventional biochemical test and 16S Rrna. Twenty-six groups of bacteria and fungi, either of human or environmental origin were detected. Microbial count ranges from 20-2056 CFU/m-3 of air, with statistical significant variation (P<0.05) across locations which is proportional to human, other biological and physical activities. Environmental agents generally predominated while significantly higher counts were detected as the level of anthropogenic activities increases. Seven genera of fungi, mainly members of the genus Aspergillum, were isolated from all locations. Results correlated with data obtained from treatment centres establishes a link between the presence of these airborne bacteria and fungi and development of respiratory diseases.
This document discusses plant virus epidemiology. It defines epidemiology as the study of disease development in plant populations over time and space, influenced by environmental and human factors. The key components of virus epidemiology are the virus, host, vector, and environment. Disease development is studied through disease progress curves and disease gradient curves. Monitoring methods include visual inspection, indexing of infected plants, and use of bait plants. Environmental factors like temperature, rainfall, and wind influence the virus, vector, and host.
Newer version is available: https://www.slideshare.net/RachelMackelprang/nove...RachelMackelprang
Based on the information provided, the scenario that would put me at the highest risk of being infected by the SARS-CoV-2 virus from the infected student in the crowded classroom would be:
- Sitting or standing very close to the infected student for a prolonged period of time, especially if we were talking face-to-face without any face masks. This would allow the virus to spread more easily through respiratory droplets released into the air by the infected student through coughing, sneezing, talking or breathing.
- Touching surfaces like desks or doorknobs that the infected student had recently touched and then touching my own eyes, nose or mouth without practicing appropriate hand hygiene in between. This could allow the virus to
The document discusses anthrax, its transmission, symptoms, and prevention. It summarizes research on the effectiveness of an anthrax vaccine, finding it over 92% effective based on a study of workers exposed at contaminated mills. Public health experts recommend mass production and distribution of the vaccine in case of a bioterrorism attack involving anthrax. The best prevention strategies involve limiting exposure opportunities through the environment and host immunity.
Coronavirus infections in children including covid 19 an overview of the epid...gisa_legal
This document summarizes epidemiological, clinical, and diagnostic findings for common circulating and novel coronaviruses that infect humans, with a focus on infections in children. It discusses four common human coronaviruses (HCoV-229E, HCoV-HKU1, HCoV-NL63, HCoV-OC43) that typically cause mild respiratory illness in children. It also describes two novel coronaviruses, SARS-CoV which emerged in 2002 causing severe acute respiratory syndrome, and MERS-CoV which emerged in 2012 causing Middle East respiratory syndrome, both of which have caused outbreaks with higher mortality rates. The document reviews symptoms, treatments, and prevention strategies for coronavirus infections in children.
Outbreak! Learning about Zika Transmission and TestingDanielle Snowflack
In this lesson, students will explore transmission and diagnosis of infectious diseases using the recent Zika outbreak as a model. First, students will use a simple model to simulate the spread of an infectious disease through a population. Next, they will use the Enzyme Linked Immunosorbent Assay (ELISA) to test patient samples for Zika. The results will be summarized in a laboratory report.
Air Microbiology. Aerobiology is defined as the study of life present in the air. Aeromicrobiology relates to the study of environmentally relevant microorganisms. ... In dry whether the microbial load of air is high while in wet weather the rain washes the microorganisms from the air.
This document provides an overview of vector control in humanitarian emergencies. It describes the public health importance of vector-borne diseases among displaced populations, characterizes common disease vectors such as mosquitoes and flies, and defines approaches for developing context-appropriate vector control strategies. The document focuses on controlling vectors through methods like indoor residual spraying and larviciding, as well as safely monitoring vector control programs.
Microbiology of Air
Aero-microbiology
Airborne diseases.
Sources of microorganisms in Air
Microbes in atmosphere
Bioaerosol
“Study of living microbes suspended in air”
Various layers present in the atmosphere at height of 1000km
Nearest to earth is troposphere
troposphere contains heavy load of microorganisms
Boundary layer responsible for transport of particles both short and long distances
Bio Aerosol
“particles release from terrestrial and marine ecosystem into the atmosphere they consist of both living and non living components
including organisms, dispersal method of organisms and excretion
List of insect vector transmitted plant diseases & TerminologyRana Salah-ud-Din
This document lists insect vectors and the plant diseases they transmit. It includes viruses that infect crops like cotton, papaya, banana, potato, tomato, chilli, rice, wheat, barley, fig, and maize. It also lists fungal diseases of mango, elm, and oak, as well as bacterial diseases of citrus, sesame, and potato. The terminology section defines terms like vector, disease, epidemiology, and Koch's postulates. It describes different modes of transmission including physical, biological, circulative, propagative, persistent, non-persistent, and semi-persistent.
This document discusses infection and infectious diseases. It begins by defining key terms like infection, acute and chronic infection. It then classifies infections in various ways such as primary vs secondary. The document outlines different sources of infection like humans, animals, insects, soil/water and food. It describes several methods by which infections can be transmitted, including contact, inhalation, ingestion and more. Finally, it discusses factors that influence microbial pathogenicity and mechanisms pathogens use to cause disease, such as adhesion, invasiveness, and survival strategies within the host.
Name : Renathan Agustianus
NIM : 20190900012
Major : Industrial Engineering
Faculty : Science and Technology
Courses : Bahasa Inggris 2
Lecturer : Harisa Mardiana
FInal Exam
1) The article investigates the chemical-physical properties of coronaviruses and how they may influence airborne transmission. Characteristics like virus size, electrical features, and envelope composition can impact diffusion properties and the virus's ability to evade the immune system.
2) Studies have shown coronaviruses have a distinct chemical-physical pattern that may help shield them and influence Brownian motion, aiding airborne spread. Their envelopes also seem to play a role in immune evasion.
3) The rapid spread of the second COVID-19 wave in some countries suggests airborne transmission may be an important hypothesis to consider in addition to direct and droplet contact. Understanding the virus's chemical-physical traits
1) A study analyzed the transmission of COVID-19 in indoor settings like hospitals in Wuhan, China. It found viral RNA in patient areas with concentrations highest in ICUs, and lower concentrations in outdoor public areas.
2) Past research on the 2003 SARS outbreak in Hong Kong found the virus spread primarily through airborne transmission in indoor apartment buildings, carried by airflow between buildings.
3) Studies of the Amoy Gardens outbreak linked greater risk of infection to middle and upper floors of buildings, and apartments facing the direction from which wind blew from an infected building. This supported the role of airborne transmission through virus-laden aerosols.
OMICS Group is an organization committed to making scientific research openly accessible. It hosts over 400 peer-reviewed open access journals and organizes over 300 international conferences annually. The organization has over 3 million readers for its journals. It aims to serve the scientific community by ensuring a rapid peer review process from its large editorial board of over 30,000 reviewers. OMICS Group also partners with over 1000 international societies to make healthcare information openly accessible. It welcomes high-quality research submissions and follows a peer review process to maintain journal excellence.
Structural Design on Virus and its Diversityijtsrd
The coronavirus disease 19 COVID 19 is a highly transmittable and pathogenic viral infection caused by severe acute respiratory syndrome coronavirus 2 SARS CoV 2 , which emerged in Wuhan, China and spread around the world. Genomic analysis revealed that SARS CoV 2 is phylogenetically related to severe acute respiratory syndrome like SARS like bat viruses, therefore bats could be the possible primary reservoir. The intermediate source of origin and transfer to humans is not known, however, the rapid human to human transfer has been confirmed widely. In this document we will analyze the structure and diversity of the pathogen and we will also discuss the previous emergence of human coronaviruses like Severe Acute Respiratory Syndrome Coronavirus SARS CoV and Middle East Respiratory Syndrome MERS CoV . Nadia Naseer "Structural Design on Virus and its Diversity" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-4 | Issue-4 , June 2020, URL: https://www.ijtsrd.com/papers/ijtsrd31225.pdf Paper Url :https://www.ijtsrd.com/biological-science/microbiology/31225/structural-design-on-virus-and-its-diversity/nadia-naseer
1) The document discusses the link between air pollution and the spread and severity of COVID-19. It analyzes literature showing that air pollutants like particulate matter can act as carriers for viral particles and other toxins, worsening the inflammatory response in lungs.
2) Areas with high air pollution were hit harder by the first wave of COVID-19 and the emergence of variants may be related to factors in polluted air acting as mutagens.
3) Reducing global air pollution is needed to curb the effects of COVID-19. The rapid spread of the virus, especially variants, suggests it can be airborne, with implications for prevention measures and international responsibility in addressing pollution.
The document discusses the relationship between COVID-19 vaccines that target the full-length spike protein versus just the receptor-binding domain (RBD), and their potential relationship to procoagulant effects and rare blood clotting issues. Some key points:
- Vaccines like AstraZeneca use the full-length spike protein in viral vectors, while others just target the RBD region.
- The spike protein plays a role in viral entry and interacts with host cell receptors like ACE2. It also has glycans that help disguise the virus.
- Studies found the AstraZeneca vaccine induces cell spikes similar to SARS-CoV-2, eliciting an immune response.
-
This document summarizes a book on the epidemiology and diffusion of viruses with a focus on the role of latitude, air pollutants, and humidity. It discusses several viruses including SARS, MERS, influenza, and COVID-19. It reviews literature finding associations between increased air pollutants like PM2.5 and higher risk of influenza-like illness. Some studies found temperature could impact COVID-19 transmission, with an optimal temperature range. Experiments with influenza in guinea pigs found that cold, dry conditions favor airborne transmission. The role of atmospheric conditions in the seasonality and spread of influenza over large geographic areas is discussed.
This is my first ever Presentation on Coronavirus Pandemic. It is made to grab attention of youngsters,school going children who might be unaware about some important facts of Corona Virus I hope this would be helpful to increase their current affairs.
This document summarizes research on the immunological and toxicological implications of COVID-19, focusing on the innate immune response and immune evasion. It discusses how the virus can trigger a "cytokine storm" through overactivation of the innate immune system and proinflammatory cytokines like IL-6. This storm can lead to widespread inflammation and multi-organ failure. The document also explores potential therapeutic strategies aimed at modulating the cytokine response, such as using corticosteroids or chloroquine to reduce IL-6 levels and calm the storm. Understanding the immune dysregulation and identifying key signaling pathways may help develop new clinical management approaches and prevent progression to severe illness.
This document discusses the field of aeromicrobiology. It defines aeromicrobiology as the study of microorganisms and their spores that are suspended in air but invisible to the naked eye. The document traces the history and evolving definition of aeromicrobiology from the 1930s to today. It also outlines different bioaerosol forms, habitats of microorganisms in the air, and factors that affect their growth. Indoor aeromicrobiology and mechanisms for controlling bioaerosols in indoor environments like laboratories are also summarized.
Activity Relatedness of Environment and Distribution of Air borne Biocontamin...Premier Publishers
Airborne indoor and outdoor bacteria and fungi were assessed during the spring season using conventional methods to investigate the enumeration and identification of airborne micro-organisms taking into consideration anthropogenic variation of the environment. This was determined through air quality sampling using microbial air sampler (M.A.Q.S.II-90, OXOID, UK). The air samples were collected during the onset of harmattan in Nov 2017. four different zones located in Owerri local Govt, were chosen for the collection of airborne bacteria and fungi. These zones were Owerri downtown (Zone A), Imo State University (Zone B), Naze building material layout (Zone C) and the Open unoccupied area along Obinze Port Harcourt road (Zone D). were selected for air bio-pollutant measurement. Cultivation and total microscopic enumeration methods were employed for the sample analysis. Identification of isolates was done using conventional biochemical test and 16S Rrna. Twenty-six groups of bacteria and fungi, either of human or environmental origin were detected. Microbial count ranges from 20-2056 CFU/m-3 of air, with statistical significant variation (P<0.05) across locations which is proportional to human, other biological and physical activities. Environmental agents generally predominated while significantly higher counts were detected as the level of anthropogenic activities increases. Seven genera of fungi, mainly members of the genus Aspergillum, were isolated from all locations. Results correlated with data obtained from treatment centres establishes a link between the presence of these airborne bacteria and fungi and development of respiratory diseases.
This document discusses plant virus epidemiology. It defines epidemiology as the study of disease development in plant populations over time and space, influenced by environmental and human factors. The key components of virus epidemiology are the virus, host, vector, and environment. Disease development is studied through disease progress curves and disease gradient curves. Monitoring methods include visual inspection, indexing of infected plants, and use of bait plants. Environmental factors like temperature, rainfall, and wind influence the virus, vector, and host.
Newer version is available: https://www.slideshare.net/RachelMackelprang/nove...RachelMackelprang
Based on the information provided, the scenario that would put me at the highest risk of being infected by the SARS-CoV-2 virus from the infected student in the crowded classroom would be:
- Sitting or standing very close to the infected student for a prolonged period of time, especially if we were talking face-to-face without any face masks. This would allow the virus to spread more easily through respiratory droplets released into the air by the infected student through coughing, sneezing, talking or breathing.
- Touching surfaces like desks or doorknobs that the infected student had recently touched and then touching my own eyes, nose or mouth without practicing appropriate hand hygiene in between. This could allow the virus to
The document discusses anthrax, its transmission, symptoms, and prevention. It summarizes research on the effectiveness of an anthrax vaccine, finding it over 92% effective based on a study of workers exposed at contaminated mills. Public health experts recommend mass production and distribution of the vaccine in case of a bioterrorism attack involving anthrax. The best prevention strategies involve limiting exposure opportunities through the environment and host immunity.
Coronavirus infections in children including covid 19 an overview of the epid...gisa_legal
This document summarizes epidemiological, clinical, and diagnostic findings for common circulating and novel coronaviruses that infect humans, with a focus on infections in children. It discusses four common human coronaviruses (HCoV-229E, HCoV-HKU1, HCoV-NL63, HCoV-OC43) that typically cause mild respiratory illness in children. It also describes two novel coronaviruses, SARS-CoV which emerged in 2002 causing severe acute respiratory syndrome, and MERS-CoV which emerged in 2012 causing Middle East respiratory syndrome, both of which have caused outbreaks with higher mortality rates. The document reviews symptoms, treatments, and prevention strategies for coronavirus infections in children.
Outbreak! Learning about Zika Transmission and TestingDanielle Snowflack
In this lesson, students will explore transmission and diagnosis of infectious diseases using the recent Zika outbreak as a model. First, students will use a simple model to simulate the spread of an infectious disease through a population. Next, they will use the Enzyme Linked Immunosorbent Assay (ELISA) to test patient samples for Zika. The results will be summarized in a laboratory report.
Air Microbiology. Aerobiology is defined as the study of life present in the air. Aeromicrobiology relates to the study of environmentally relevant microorganisms. ... In dry whether the microbial load of air is high while in wet weather the rain washes the microorganisms from the air.
This document provides an overview of vector control in humanitarian emergencies. It describes the public health importance of vector-borne diseases among displaced populations, characterizes common disease vectors such as mosquitoes and flies, and defines approaches for developing context-appropriate vector control strategies. The document focuses on controlling vectors through methods like indoor residual spraying and larviciding, as well as safely monitoring vector control programs.
Microbiology of Air
Aero-microbiology
Airborne diseases.
Sources of microorganisms in Air
Microbes in atmosphere
Bioaerosol
“Study of living microbes suspended in air”
Various layers present in the atmosphere at height of 1000km
Nearest to earth is troposphere
troposphere contains heavy load of microorganisms
Boundary layer responsible for transport of particles both short and long distances
Bio Aerosol
“particles release from terrestrial and marine ecosystem into the atmosphere they consist of both living and non living components
including organisms, dispersal method of organisms and excretion
List of insect vector transmitted plant diseases & TerminologyRana Salah-ud-Din
This document lists insect vectors and the plant diseases they transmit. It includes viruses that infect crops like cotton, papaya, banana, potato, tomato, chilli, rice, wheat, barley, fig, and maize. It also lists fungal diseases of mango, elm, and oak, as well as bacterial diseases of citrus, sesame, and potato. The terminology section defines terms like vector, disease, epidemiology, and Koch's postulates. It describes different modes of transmission including physical, biological, circulative, propagative, persistent, non-persistent, and semi-persistent.
This document discusses infection and infectious diseases. It begins by defining key terms like infection, acute and chronic infection. It then classifies infections in various ways such as primary vs secondary. The document outlines different sources of infection like humans, animals, insects, soil/water and food. It describes several methods by which infections can be transmitted, including contact, inhalation, ingestion and more. Finally, it discusses factors that influence microbial pathogenicity and mechanisms pathogens use to cause disease, such as adhesion, invasiveness, and survival strategies within the host.
Name : Renathan Agustianus
NIM : 20190900012
Major : Industrial Engineering
Faculty : Science and Technology
Courses : Bahasa Inggris 2
Lecturer : Harisa Mardiana
FInal Exam
Similar to luisetto m et al bioaeresols and corona-virus diffusion, transmission , carriers, viral size, surfaces properties and other factor involved.
1) The article investigates the chemical-physical properties of coronaviruses and how they may influence airborne transmission. Characteristics like virus size, electrical features, and envelope composition can impact diffusion properties and the virus's ability to evade the immune system.
2) Studies have shown coronaviruses have a distinct chemical-physical pattern that may help shield them and influence Brownian motion, aiding airborne spread. Their envelopes also seem to play a role in immune evasion.
3) The rapid spread of the second COVID-19 wave in some countries suggests airborne transmission may be an important hypothesis to consider in addition to direct and droplet contact. Understanding the virus's chemical-physical traits
1) A study analyzed the transmission of COVID-19 in indoor settings like hospitals in Wuhan, China. It found viral RNA in patient areas with concentrations highest in ICUs, and lower concentrations in outdoor public areas.
2) Past research on the 2003 SARS outbreak in Hong Kong found the virus spread primarily through airborne transmission in indoor apartment buildings, carried by airflow between buildings.
3) Studies of the Amoy Gardens outbreak linked greater risk of infection to middle and upper floors of buildings, and apartments facing the direction from which wind blew from an infected building. This supported the role of airborne transmission through virus-laden aerosols.
The Sky Gets Dark Slowly in View of the Emergence of “The Super Omicron Varia...IIJSRJournal
The new Omicron variant of SARS-cov-2 speeding around the world may bring another wave of chaos. It is like walking on a tight rope. The earth is brimming with viruses. The lungs are the paramount respiratory organs. Besides COVID-19, Omicron variant, pollution is another uninvited guest. People are aggrieved by the recurrence of pollution every year. Delhi and most of the cities expressed air emergency. Nothing seems to change. Not a soul is taken care of. The lungs are the organs most affected by COVID‐19. Virus-infected patients are suffering from air hunger. COVID-19 and its variants are the lung annihilate viruses that traumatically lead to lung failure. COVID 19 pandemic sweeps across the globe, Co-infection with respiratory viruses and SARS-CoV-2 and the mutant variants Omicron, raising danger bells around the world. Omicron is an exciting outstanding pandemic co-infected with respiratory viruses, demands crucial public health Intervention.
Airborne COVID Study (2020) - Texas A&M, The University of Texas at Austin, C...chaganomics
Identifying airborne transmission as the dominant route for the spread of COVID-19. Various mitigation measures have been implemented to fight the coronavirus disease 2019 (COVID-19) pandemic, including widely adopted social distancing and mandated face covering.
This document provides a brief review of contemporary studies on SARS-CoV-2. It discusses the viral classification of SARS-CoV-2 and notes it was first reported in Wuhan, China in 2019. Research has shown SARS-CoV-2 spreads through the air making it more rapidly transmissible between humans. The document also summarizes that the steroid dexamethasone was the first drug shown to reduce deaths from COVID-19 according to a UK clinical trial. Additionally, the humanized monoclonal antibody itolizumab was approved for treating cytokine release syndrome in India. Masks are discussed as providing a critical barrier in reducing transmission when properly fitted by lowering viral concentrations exhaled by asymptomatic and symptomatic individuals.
This document summarizes a research paper about identifying the natural reservoir of SARS-CoV. It discusses how SARS emerged in China in 2002 and was later identified as a novel coronavirus. Retrospective studies found no prior antibodies in humans, indicating animal-to-human transmission. Researchers later discovered that bats are natural reservoirs of SARS-like viruses, providing evidence that SARS-CoV is a zoonotic virus of wildlife origin. Early investigations identified SARS-like coronaviruses in masked palm civets and raccoon dogs at a wild animal market, but bats are believed to be the ultimate natural reservoir.
Key question:
Could the plague ever re-emerge on a similar level in the twenty-first century?
Due to the potential seriousness of the disease this is a subject worthy of epidemiological consideration and research.
The novel coronavirus disease
(COVID-19), caused by SARS-CoV2 in 2021, is a tsunami like coronavirus
outbreak relative to the previous
outbreaks involving older coronavirus
or Swine flu (h1n1) or Spanish flu.
With the number of COVID-19 cases
now exploding worldwide, it is certain
that transmission of SARS-CoV-2 is
very high possibly airborne (aerosol
droplet) and there are diverse spectrum
of disease severity. The biological
issues like a genetic susceptibility
and variability in the response to
the virus are still being elucidated.
Controlling current rates of infection
and combating future waves require
a better understanding of the routes
of exposure to SARS-CoV-2 and the
underlying genomic susceptibility
to this disease. How individuals
respond to SARS-CoV-2 exposure
is becoming better understood in
a global sense, but differences in
the vulnerability of individuals to
infection and in the spectrum of
COVID-19 symptoms remain to be
understood. It is known that advanced
age and pre-existing conditions (e.g.
metabolic, cardiovascular, pulmonary,
and renal diseases) render a person
Plague : Medical Management and original method of treatment.Dmitri Popov
A new effective method of coutermeasure against biological weapons, antiviral treatment of acute and chronic viral hepatitis B and C and against other viral diseases was used in medical practice in hospitals.Research results show this method as effective method against severe viral infections, warfare, and outbreak infections, Biological warfare, methicillin-resistant staphylococcus aureus.
Introduction
History
Definition
Aerobiological pathway
Fundamentals of Aerobiology
New techniques for advancing aerosol science and aerobiology
Airborne Diseases
Conclusion
The document discusses chicken (poultry) as an animal model for studying human diseases like coronaviruses. It notes that the first coronavirus discovered was the avian infectious bronchitis virus (IBV) in chickens in the 1930s. Drawing from decades of experience with IBV, the poultry industry can provide insights into zoonotic coronaviruses like SARS-CoV-2 that cause COVID-19. The document outlines similarities between IBV and human coronaviruses in terms of structure, genome and replication mechanisms.
This document discusses biological agents that could potentially be used for biological warfare or terrorism. It defines biological agents and provides examples like anthrax, smallpox, plague, botulism, and tularemia. It describes the causes, methods of dissemination, history of use, and impacts of biological agents. The document emphasizes that biological weapons could potentially cause mass casualties and civil disruptions. It stresses the importance of preparedness through early diagnosis, surveillance, immunization, and enhancing clinical knowledge.
Microsporidia are obligate intracellular parasites with spores that range from 1-20 micrometers in length. They infect host cells through a unique polar tube structure. The microsporidian life cycle involves the spore injecting its contents into the host cell, proliferating within the cell, and then releasing new spores to infect other cells. Epidemiological data shows human microsporidia infections are often underreported but can be found worldwide, with transmission occurring through oral-fecal routes, inhalation, or possibly water sources. The host immune response plays a key role in controlling microsporidian infections.
The document provides an overview of coronavirus disease 2019 (COVID-19). It discusses the definition, epidemiology, virology, transmission, pathophysiology, clinical features, risk factors, diagnosis, treatment, and prevention of COVID-19. Key points include that COVID-19 is caused by the SARS-CoV-2 virus, it was first identified in Wuhan, China in 2019 and has since spread globally resulting in a pandemic, transmission occurs via respiratory droplets, clinical features range from mild to severe/critical illness, risk factors include older age and comorbidities, diagnosis involves nucleic acid or antigen testing, and treatment is generally supportive.
—Fungal organisms are ubiquitous. A common location for these organisms to enter the human body is through the external acoustic canal, oral cavity, and pharynx and sino-nasal cavity. A study was conducted with clinical and mycological analysis of various fungal infections in ENT. Patients suspected for having fungal infections attending at Department of ENT were interrogated and analysed. Swabs collected from these cases were sent for direct microscopy by KOH mounts for fungal examination and fungal culture. Microbiological confirmed 100 cases were finally included in the study Histopathological examination of nasal mass and polyposis was also done. It was observed in this present study otomycosis was most common and accounted for 84% of the total cases followed by candidiasis in oral cavity and pharynx in 9%, allergic fungal rhinosinusitis in 4% and rhinosporidiosis in 3%. Aspergillus niger was that most common fungus isolated in 61% cases, followed by Candida albicans in 24% cases, Aspergillus flavus in 9% cases, Aspergillus fumigatus and Rhinosporodium seeberi in 3% cases each. All the cases of fungal infection of oral cavity and oropharynx were due to Candida albicans.
This document discusses factors responsible for emerging and re-emerging infectious diseases. It argues that while pathogens, hosts, vectors, and environment have traditionally been seen as the main determinants of infectious disease emergence and transmission, human intervention through progress in science and technology should be considered a fifth key determinant. It provides examples of how various aspects of scientific and technological progress, such as intensive agriculture, antibiotic overuse, bioterrorism, and changes in food processing and transportation, have contributed to disease emergence and spread in recent decades.
Corona virus current scenario (theoretical outlook)Dr. sreeremya S
This document discusses the coronavirus (COVID-19) pandemic. It provides background on coronaviruses, noting they were first identified in 1960 and can cause respiratory illnesses like SARS. The current coronavirus emerged in China in late 2019 and has since spread globally, killing hundreds daily. While studies on animals are limited, research aims to develop treatments like RNA silencing. Prevention efforts are led by CDC and WHO. Coronaviruses can spread through airborne droplets and contact with infected animals or humans. Further research is needed to fully understand transmission and develop effective treatments.
The document discusses aeromicrobiology, which is the study of airborne microorganisms and their effects on human health and the environment. It defines aeromicrobiology and describes the various microbes that can be found in air, such as viruses, bacteria, fungi, and protozoa. The document also discusses how these microbes can be transmitted through the air and cause diseases in humans and other organisms. It provides examples of common airborne pathogens and the diseases they cause. Furthermore, the document discusses the sizes of airborne biological particles known as bioaerosols and different methods for sampling and analyzing bioaerosols in air, including various impactor and impinger sampling devices.
Similar to luisetto m et al bioaeresols and corona-virus diffusion, transmission , carriers, viral size, surfaces properties and other factor involved. (20)
When I was asked to give a companion lecture in support of ‘The Philosophy of Science’ (https://shorturl.at/4pUXz) I decided not to walk through the detail of the many methodologies in order of use. Instead, I chose to employ a long standing, and ongoing, scientific development as an exemplar. And so, I chose the ever evolving story of Thermodynamics as a scientific investigation at its best.
Conducted over a period of >200 years, Thermodynamics R&D, and application, benefitted from the highest levels of professionalism, collaboration, and technical thoroughness. New layers of application, methodology, and practice were made possible by the progressive advance of technology. In turn, this has seen measurement and modelling accuracy continually improved at a micro and macro level.
Perhaps most importantly, Thermodynamics rapidly became a primary tool in the advance of applied science/engineering/technology, spanning micro-tech, to aerospace and cosmology. I can think of no better a story to illustrate the breadth of scientific methodologies and applications at their best.
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We will metaverse into the essence of immersive learning, into its three dimensions and conceptual models. This approach encompasses elements from teaching methodologies to social involvement, through organizational concerns and technologies. Challenging the perception of learning as knowledge transfer, we introduce a 'Uses, Practices & Strategies' model operationalized by the 'Immersive Learning Brain' and ‘Immersion Cube’ frameworks. This approach offers a comprehensive guide through the intricacies of immersive educational experiences and spotlighting research frontiers, along the immersion dimensions of system, narrative, and agency. Our discourse extends to stakeholders beyond the academic sphere, addressing the interests of technologists, instructional designers, and policymakers. We span various contexts, from formal education to organizational transformation to the new horizon of an AI-pervasive society. This keynote aims to unite the iLRN community in a collaborative journey towards a future where immersive learning research and practice coalesce, paving the way for innovative educational research and practice landscapes.
Unlocking the mysteries of reproduction: Exploring fecundity and gonadosomati...AbdullaAlAsif1
The pygmy halfbeak Dermogenys colletei, is known for its viviparous nature, this presents an intriguing case of relatively low fecundity, raising questions about potential compensatory reproductive strategies employed by this species. Our study delves into the examination of fecundity and the Gonadosomatic Index (GSI) in the Pygmy Halfbeak, D. colletei (Meisner, 2001), an intriguing viviparous fish indigenous to Sarawak, Borneo. We hypothesize that the Pygmy halfbeak, D. colletei, may exhibit unique reproductive adaptations to offset its low fecundity, thus enhancing its survival and fitness. To address this, we conducted a comprehensive study utilizing 28 mature female specimens of D. colletei, carefully measuring fecundity and GSI to shed light on the reproductive adaptations of this species. Our findings reveal that D. colletei indeed exhibits low fecundity, with a mean of 16.76 ± 2.01, and a mean GSI of 12.83 ± 1.27, providing crucial insights into the reproductive mechanisms at play in this species. These results underscore the existence of unique reproductive strategies in D. colletei, enabling its adaptation and persistence in Borneo's diverse aquatic ecosystems, and call for further ecological research to elucidate these mechanisms. This study lends to a better understanding of viviparous fish in Borneo and contributes to the broader field of aquatic ecology, enhancing our knowledge of species adaptations to unique ecological challenges.
Or: Beyond linear.
Abstract: Equivariant neural networks are neural networks that incorporate symmetries. The nonlinear activation functions in these networks result in interesting nonlinear equivariant maps between simple representations, and motivate the key player of this talk: piecewise linear representation theory.
Disclaimer: No one is perfect, so please mind that there might be mistakes and typos.
dtubbenhauer@gmail.com
Corrected slides: dtubbenhauer.com/talks.html
Remote Sensing and Computational, Evolutionary, Supercomputing, and Intellige...University of Maribor
Slides from talk:
Aleš Zamuda: Remote Sensing and Computational, Evolutionary, Supercomputing, and Intelligent Systems.
11th International Conference on Electrical, Electronics and Computer Engineering (IcETRAN), Niš, 3-6 June 2024
Inter-Society Networking Panel GRSS/MTT-S/CIS Panel Session: Promoting Connection and Cooperation
https://www.etran.rs/2024/en/home-english/
aziz sancar nobel prize winner: from mardin to nobel
luisetto m et al bioaeresols and corona-virus diffusion, transmission , carriers, viral size, surfaces properties and other factor involved.
1. InternationalInventionof ScientificJournal
Available Online at http://www.iisj.in
eISSN: 2457-0958
Volume 05|Issue 01|January, 2021|
Page | 1
Bioaeresols and corona-virus diffusion, transmission , carriers, viral size, surfaces
properties and other factor involved.
Authors :
1) Luisetto m IMA academy Marijnskaya NATURAL SCIENCE Branch Italy 29121
2) Nili B. A, Innovative Pharmaceutical product development specialist, USA
3) Khaled Edbey professor University of Benghazi dep of chemistry
4) Mashori G.R. Professor Department of Medical & Health Sciences for Woman, Peoples University
of Medical and Health Sciences for Women,Pakistan
5) Ahmed YesviRafa , Founder and President, Yugen Research Organization; Undergraduate Student,
Western Michigan University, MI, USA 49008
6) Oleg YurevichLatishev IMA ACADEMY president , RU
Article Received 25-11-2020, Accepted 01-01-2021 , Published 13-01-2021
Abstract
Aim of this work is to evaluate the chemical-physical binding between some respiratory virus and
their carrier like particulate matter, water particles dust and other . This make possible to verify if
a virus is airborne or not and the forces that regulate the bioaereosol containing virus. The
corona-virus envelope whit their chemical -physical properties and electrical charge are
fundamental to fully understand properties, diffusion pattern, surviving and other relevant in
spread of this respiratory disease. Medicine , biology but also chemistry and physics can help in
fight with such Pandemic disaster .The result of this study are then useful in preventing strategy by
public international health institution.
KEYWORDS: bio-aerosols, corona-virus, covid-19, airborne, transmission Viral size, carriers
Introduction
Since the first stages of life evolution In the
earth bio-aereosols was an crucial
environment . This kind of environment
follow chemical-physics rules and are
subjected to perturbation of this property.
This bio-aereosols contain various form of life
sine also batceria , fungi but also virus. Actual
Covid-19 pandemia must to be analyzed
under this point of view : Anbioaeresols that
follow chemical-physics law. The rapid and
logarithmic explosion of cases in the second
wave in France, UK, Spain in a few time (
weeks) seem to show that no only
transmission By direct contact and by
droplets: also airborne must to be deeply
revalued. The lower PM particle in example
penetrate inside deeply in the lungs then
higher and this factor can be related to the
severity of the disease. But this chemical
2. International Invention of Scientific Journal Vol 05, Issue 01 Jan - 2021 Page | 2
physics properties of the link between virus
and carrier in Aereosol are correctly taken in
consideration in the preventing strategies?
Chemical physics properties of virus envelope
can be responsible of the link with carrier but
also to repulsion between virus increasing
Brownian moto.
According Daniel Verreault et al : related the
methods for sampling of airborne viruses:
“Any microorganism, including viruses, can
become airborne. Contaminated material can
be aerosolized in many different ways,
ranging from wind to human and animal
activities such as sneezing, mechanical-
processes, etc. If the aero-dynamic size of an
infectious particle is appropriate, it can
remain airborne, come into contact with
humans or animals, and potentially cause an
infection. The probability of an airborne
micro-organism-laden particle causing an
infection depends on its infectious- potential
and its ability to resist the stress of
aerosolization. Many epidemiological- studies
have proposed that viruses can spread from
one host to another by using air for
transport. The capacity of the foot-and-
mouth disease (FMD) virus to spread by air
has been studied and reviewed over the
years and is now being investigated using
computer- models. One of these models
predicted that in a “worst-case scenario” of
an FMD outbreak, cattle could be infected as
far as 20 to 300 kilometers away from an
infectious source . Dispersion- models based
on meteorological -data and information on
the spread of FMD at the beginning of the
1967-68 epidemic in the UK strongly
suggested that the infection may have spread
by the airborne- route over a distance of 60
km . Airborne- transmission of FMD was also
reported to have occurred during the 1982-
1983 epidemic in Denmark. In the latter case,
an analysis of epidemiological dynamics using
molecular- methods coupled with
meteorological data concluded that the
infection had spread by air over a distance of
70 km . Similarly, the results of a Canadian
study on an FMD epidemic reported that
airborne- viruses may have traveled 20 km
down-wind from the contaminated source . a
recent study on the O/UKG/2001 strain of
FMD virus indicated that it does not spread
efficiently between sheep by the airborne-
route.
Other strains may behave differently .Air
borne micro-organisms can represent major
health and economic- risks to human and
animal -populations. Appropriate preventive
actions can be taken if the threat posed by
such micro-organisms is better understood.
Authorities need to be aware of the nature,
concentration, and pathogenicity of airborne-
micro-organisms to better control them. This
information can be obtained by using various
air sampling -methods, each of which has its
particular advantages and disadvantages.
Many types of samplers and analytical
methods have been used over the years
A virus can multiply only within a host- cell.
Infected cells can spread viruses directly into
the surrounding- air (primary- aerosolization)
or to fluids and surfaces, which can become
sources for airborne- transmission
(secondary aerosolization). Secondary
aerosolization can occur for any virus,
predominantly when air displacements or
movements around contaminated surfaces or
fluids disperse the viruses into the air. It can
also occur by liquid- splashes, which can
aerosolize viruses in liquids or on surfaces.
3. International Invention of Scientific Journal Vol 05, Issue 01 Jan - 2021 Page | 3
Almost any kind of disturbance of infected
organisms or materials, even the bursting of
bubbles in sea-water , can produce airborne,
virus-laden particles. Farm- animals have also
been studied for their emission of airborne -
viruses. The FMD virus, which is one of the
most widely studied airborne animal- viruses,
has been detected in air contaminated by
infected- pigs and ruminants in both
laboratory settings and farm environments.
This single-stranded RNA (ssRNA) virus of
the Picornaviridae family is excreted in all
body fluids of infected animals and can
become airborne directly from the animals or
from the secondary aerosolization of
deposited- viruses or virus-laden particles.
Other suspected sources of airborne -viruses,
such as burning carcasses of infected animals
, have not yet been identified formally as true
sources because additional investigations are
needed.
Poultry farms are also potential producers of
virus-laden airborne- particles. The exotic
Newcastle- disease virus
(Paramyxoviridae family) was probably the
first virus isolated from a naturally
contaminated environment of poultry-
houses sheltering infected birds. This 150-
nm-diameter ssRNA virus was detected in air
samples from 2 farms during an outbreak in
Southern- California in 2002-2003 . Air
samples in and around broiler poultry-
houses have also been studied for the
presence of viruses such as Escherichia-
coli bacteriophages, which are a fecal-
contamination tracer . Other animals, such as
bats (rabies- virus) , rabbits (rabbit poxvirus) ,
and mice (polyomavirus) , have been studied
as sources of bio-aerosols. These viruses can
be released into the air directly from animals
by their breathing, coughing, and sneezing or
by secondary- aerosolization. It should be
noted that the means of aerosolization has a
critical impact on the aerodynamic- size and,
thus, on the behavior of the airborne-
particles. Given that virus-laden particles are
a complex mixture of various components
(salts, proteins, and other organic and
inorganic- matter, including virus- particles),
it is essential to realize that the size of the
viral -particle itself does not rule the
airborne-particle-size.
Another study investigating pigs- infected
with Aujeszky's disease- virus
(Herpesviridae family; about 150 nm in
diameter; double-stranded DNA [dsDNA]
virus) found that the infectivity of the
aerosols collected in each stage of the three-
stage impinger varied over time. The
investigators reported that the size-
distributions of the aerosols in the three
stages were comparable on day 2 of the
infection but that there was an increase in
infectivity associated with larger- particles on
days 3 and 4 . no clear association has been
made between aerosol- infectivity and a
particular size range. While single virus -
particles exist in the air , they tend to
aggregate rapidly. Aggregation speed
depends on the size- distribution of the
airborne particles, the concentration of the
aerosol, and the thermo-dynamic conditions .
infectious droplets exhaled by animals shrink
rapidly with the lower- humidity outside the
respiratory -airway, creating smaller aerosols.
the size distribution of such naturally
generated bio-aerosols depends on the sizes
of the particles to which the micro-organisms
bind. This binding may occur by diffusion,
impaction, interception, or electrostatic -
attraction.
4. International Invention of Scientific Journal Vol 05, Issue 01 Jan - 2021 Page | 4
Larger- particles may be relatively less
hazardous than smaller ones. It has been
shown on pig farms that a visually clean
environment may be more contaminated by
bio-aerosols than a visually dirty one .” (1)
Table n 1 Effects of RH relative humidity on infectivity of a selection of airborne viruses
Virus
Optimal RH for
maximum infectivity Family Geneticmaterial
Size
(nm) Envelope
Influenza virus Low Orthomyxoviridae ssRNA (−) 80-120 Yes
Newcastle disease virus Low Paramyxoviridae ssRNA (−) 150 Yes
Vesicularstomatitis virus Low Rhabdoviridae ssRNA (−) 60 × 200 Yes
Japaneseencephalitis virus Low Flaviviridae ssRNA (+) 40-60 Yes
Porcine reproductive and respiratory
syndrome virus
Low Arteriviridae ssRNA (+) 45-60 Yes
SemlikiForest virus Low Togaviridae ssRNA (+) 70 Yes
Human corona-virus 229E Mid-range Coronaviridae ssRNA (+) 120-160 Yes
Rotavirus Mid-range Reoviridae dsRNA 100 No
5. International Invention of Scientific Journal Vol 05, Issue 01 Jan - 2021 Page | 5
Pseudorabies virus Mid-range Herpesviridae dsDNA 200 Yes
Rhinovirus High Picornaviridae ssRNA (+) 25-30 No
Poliovirus High Picornaviridae ssRNA (+) 25-30 No
Picornavirus High Picornaviridae ssRNA (+) 25-30 No
T3 coliphage High Podoviridae dsDNA 60
(capsid)
No
Rhinotracheitis virus High Herpesviridae dsDNA 200 Yes
St. Louis encephalitis virus All Flaviviridae ssRNA (+) 40-60 Yes
“To our knowledge, the oldest study on the sampling of airborne -viruses was performed with a laboratory
setup using a chamber and an artificially produced aerosol of influenza- virus “(1)
M K Ijaz et al :
“The survival of airborne human-corona-virus
229E (HCV/229E) was studied under different
conditions of temperature (20 +/- 1 degree C
and 6 +/- 1 degree C) and low (30 +/- 5%),
medium (50 +/- 5%) or high (80 +/- 5%)
relative-humidities (RH). At 20 +/- 1 degree C,
aerosolized HCV/229E was found to survive
best at 50% RH with a half-life of 67.33 +/-
8.24 h while at 30% RH the virus half-life was
26.76 +/- 6.21 h. At 50% RH nearly 20%
infectious- virus was still detectable at 6 days.
High RH at 20 +/- 1 degree C, it was found to
be the least-favourable to the survival of
aerosolized- virus and under these conditions
the virus half-life was only about 3 h; no any
6. International Invention of Scientific Journal Vol 05, Issue 01 Jan - 2021 Page | 6
virus could be detected after 24 h in aerosol.
At 6 +/- 1 degree C, in either 50% or 30% RH-
conditions, the survival of HCV/229E was
significantly- enhanced, with the decay -
pattern essentially similar to that seen at 20
+/- 1 degree C. At a low- temperature and
high RH (80%), the survival pattern was
completely reversed, with the HCV/229E half-
life increasing to 86.01 +/- 5.28 h, nearly 30
times that found at 20 +/- 1 degree C and
high RH. Although optimal survival at 6
degree C still occurred at 50% RH, the
pronounced stabilizing effect of low -
temperature on the survival of HCV/229E at
high RH indicates that the role of the
environment on the survival of viruses in air
may be more complex and significant than
previously thought.” (2)
Figure n. 1
As showed in this figure aereosol physics cover different scientific discipline like Medicine, biology,
chemistry and also physics. It is not possible to study a respiratory virus disease without not
consider also chemical physical properties in the interfaces of interaction virus – carriers.
7. International Invention of Scientific Journal Vol 05, Issue 01 Jan - 2021 Page | 7
Fig. n 2
Related Bioaeresols some references are of interest for the scope of this work:
according E. Domingo:
A primitive RNA world
“A simplified -overview of a course of events
that led to the origin of biological- systems is
depicted in Fig. 3 . The prebiotic synthesis of
potential building blocks—which might have
been initiated earlier than about 5000
million years ago—renders plausible the
existence of a pre-RNA era that was then
replaced by an RNA- world in the late Hadean
early Archean periods on Earth. This stage
should have been followed by one in which
RNA was complemented by DNA as a
repository of genetic--information . Polymers
other than DNA and RNA are also capable of
encoding evolvable inheritable-information .
Heterogeneous nucleic- acid molecules
(including mixtures of ribo- and deoxyribo-
polymers) can give rise to functional nucleic
acids .RNA-enzymes (ribozymes), such as RNA
ligases can evolve from random-sequence
RNAs . The critical polymerization -reaction
involves the formation of a phosphor-diester
bond and release of pyro-phosphate—
analogous to the reactions catalyzed by the
present-day RdRps—and represent an
incipient, primitive-anabolism . In support of
a possible link between catalytic RNA-
activities and solid mineral- surfaces in the
origin of life, the catalytic activity of the
hammerhead ribozyme of the Avocado Sun
Blotch viroid was maintained when bound to
the clay mineral montmorillonite .
Theories of the origins of viruses
Although not in a linear fashion, the number
of nucleotides or base pairs in the genetic-
material—that presumably reflects the
amount of genetic- information relevant to
confer phenotypic traits—increased as
evolution led to differentiated organisms.
The major theories of the origin of viruses are
divided into 2 opposite categories: those that
attribute virus- origins to the early
development of life, and those that propose
that viruses arose when a cellular- life was
already in place .
These 2 broad views that we may term
“viruses without cells” and
“viruses from cells” are not irreconcilable,
although reconstruction of ancestral-
developments is challenging. They can be
divided into 5 main theories—not all
independent or mutually exclusive— which
are summarized next.” (3)
8. Page | 8
fig. n 3 Two possible courses of events regarding
when viruses first appeared and participated in
the evolution of the biosphere. The scheme of
time frames and major biological events (RNA-
world, first cells, and organisms) are those
displayed in Fig. 3. According to the upper-
diagram, viruses (or previrus-like entities) arose
together with the first (pre-cellular) replicating
entities. According to the second diagram, viruses
(or pre-virus-like entities) arose when a cellular
life had already been established. Presence of
virus is generically represented by the external,
thick, black curves. The internal red, wavy lines
represent generation, dominance, and extinction
of multiple viral- lineages whose numbers and
true dynamics will remain unknown and in
article Airborne Infectious Micro-organisms
L.D. Stetzenbach, in Encyclopedia of
Microbiology (Third Edition), 2009
Bio-aerosols
“A bio-aerosol is an airborne collection of
biological- material. Bio-aerosols can be
comprised of bacterial -cells and cellular
fragments, fungal spores and fungal hyphae,
viruses, and by-products of microbial-
metabolism. Pollen grains and other
biological material can also be airborne as a
bio-aerosol. Microbial- aerosols are
generated in outdoor and indoor-
environments as a result of a variety of
natural and anthroprogenic- activities. Wind,
rain and wave splash, spray irrigation, waste-
water treatment activity, cooling towers and
air handling water spray systems, and
agricultural processes such as harvesting and
tilling are examples of activities that generate
bio-aerosols outdoors. Indoors bio-aerosols
are generated and dispersed mechanical and
human -activity. Industrial and manufacturing
practices and bio-fermentation procedures
can generate high concentrations of microbial
-aerosols. Heating, ventilation, and air
conditioning (HVAC) systems, water spray
devices ( showerheads and humidifiers), and
cleaning (- dusting, sweeping, vacuuming, and
mopping) result in the transport of microbial
9. International Invention of Scientific Journal Vol 05, Issue 01 Jan - 2021 Page | 9
-materials in the air. Talking and coughing
generate bio-aerosols from individuals, some
of which may be infectious. Facilities with
medical, dental, or animal care practices can
generate infectious microbial- aerosols
The individual particle-size of particulate-
material in bio-aerosols is generally 0.3–
100 μm in diameter; larger particles tend to
settle rapidly and are not readily transported
in the air. Virus particles are nanometer in
size, bacterial cells are approximately 1 μm in
diameter, and fungal spores are >1 μm.
These micro-organisms can be dispersed in
the air as single -units, but are often present
as aggregate -formations. The larger -
aggregates have different aerodynamic
properties than single-cell units; therefore
their dispersal may be different than single-
unit particles. Aggregates of biological-
material also afford protection from
environmental- stresses such as desiccation,
and exposure to ultraviolet radiation ozone
and other pollutants in the atmosphere.
Often bacterial -cells and virus particles are
associated with skin cells, dust, and other
organic or inorganic- material. During
agricultural practices ( during harvesting, and
tilling), fungus spores are released from plant
surfaces and the soil and raft on
other particulate- matter. This ‘rafting’
affects the aerodynamic characteristics and
the survival of the cells in the bio-aerosol.
When biological material is dispersed from
wate-r sources ( splash, rainfall, or cooling
towers and fountains), it is generally
surrounded by a thin layer of water. This
moisture layer also changes the aero-dynamic
properties and aids in the survivability of the
micro-organisms while airborne. Airborne-
particulate will remain airborne until settling
occurs or they are inhaled. Following
inhalation, large airborne particles are lodged
in the upper respiratory tract ( nose and
naso-pharynx). Particles <6 μm in diameter
are transported to the lung where the 1–
2 μm particles have the greatest retention in
the alveoli.”
12. International Invention of Scientific Journal Vol 05, Issue 01 Jan - 2021 Page | 12
Fig. n 7 Schematics of the experimental
setup . (a) Experiments are conducted in a
bio-safety cabinet to keep all aerosolized-
virus contained. The setup includes a
Collison- nebulizer to aerosolize using
compressed- air and viral solutions provided
by a syringe pump. Tubing directs the
airborne viruses into the sampling unit (b):
(1) our ESP sampler or (2) gelatin- filters for
comparison. A down-stream vacuum pump
maintains a constant flow and under
pressure inside the ESP sampler. (b) Details of
the sampling units: (1) Our ESP sampler
includes a three-electrode corona discharge
electrostatic- precipitator to capture the
aerosol particle directly into an integrated
liquid collector with a miniaturized -volume
of 150 μL; (2) gelatin filters are used for
comparative measurement of the total
amount of virus effectively entering the ESP -
sampler after nebulization. The filters are
housed in a specific cassette placed at the
aerosol -inlet.Ladhani L, Pardon G, Meeuws
H, van Wesenbeeck L, Schmidt K, Stuyver L,
et al. (2017) Sampling and detection of
airborne influenza virus towards point-of-
care applications.
PrakritiSharmaGhimire et al :
“Bio-aerosols such as airborne- bacteria,
fungal spores, pollen, and others possess
diverse characteristics and effects. A large
gap exists in the scientific understanding of
the overall physical- characteristics and
measurement of bio-aerosols. Consequently,
this review study aims to devise an
appropriate approach to generate more
scientific knowledge of bio-aerosols.” (4)
Beijing [China]: Local- authorities have said
that the novel corona-virus can spread
through direct transmission, contact-
transmission, or aerosol -transmission.
Shanghai officials reveal novel corona-virus
transmission modesBy Zhou Wenting in
Shanghai | chinadaily.com.cn | Updated:
2020-02-08 “Confirmed transmission- routes
13. International Invention of Scientific Journal Vol 05, Issue 01 Jan - 2021 Page | 13
of the novel-corona-virus include direct
transmission, contact transmission and
aerosol -transmission, a Shanghai official said
on Saturday.
“Aerosol transmission refers to the mixing of
the virus with droplets in the air to form
aerosols, which causes infection after
inhalation, according to medical experts,”
said Zeng Qun, deputy- head of the Shanghai
Civil Affairs Bureau.”
According the new York times 5 oct 2020
Apoorva Mandavilli:
239 Experts With One Big Claim: The
Corona-virus Is Airborne
“The W.H.O. has resisted mounting evidence
that viral- particles floating indoors are
infectious, some scientists say. The agency
maintains the research is still in-conclusive”.
It Is Time to Address Airborne Transmission
of Corona-virus Disease 2019 (COVID-19)
Lidia Morawska et al :
“We appeal to the medical-community and
to the relevant national and international
bodies to recognize the potential for airborne
spread of corona-virus disease 2019 (COVID-
19). There is significant potential for
inhalation -exposure to viruses in
microscopic- respiratory droplets (micro-
droplets) at short to medium distances (up to
several meters, or room scale), and we are
advocating for the use of preventive -
measures to mitigate this route of airborne-
transmission.
Studies by the signatories and other
researcher- scientists have demonstrated
beyond any reasonable doubt that viruses
are released during exhalation, talking, and
coughing in micro-droplets small enough to
remain aloft in air and pose a risk of exposure
at distances beyond 1–2 m from an infected-
individual . at typical indoor-air velocities , a
5-μm droplet will travel tens of meters, much
greater than the scale of a typical room,
while settling from a height of 1.5 m to the
floor. Several retrospective-studies
workconducted after the severe acute
respiratory syndrome corona-virus 1 (SARS-
CoV-1) epidemic demonstrated that
airborne- transmission was the most likely
mechanism explaining the spatial- pattern of
infections . Retrospective- analysis has shown
the same for severe -acute respiratory
syndrome corona-virus 2 (SARS-CoV-2) . In
particular, a study in their review of records
from a Chinese- restaurant observed no
evidence of direct or indirect -contact
between the 3 parties . In their review of
video records from the restaurant, they
observed no evidence of direct or indirect -
contact between the 3 parties.
Many research studies conducted on the
spread of other viruses, including respiratory
syncytial virus (RSV) , Middle East Respiratory
Syndrome Corona-virus (MERS-CoV) , and
influenza , show that viable airborne- viruses
can be exhaled and/or detected in the
indoor- environment of infected patients .
This poses the risk that people sharing such
environments can potentially inhale these
viruses, resulting in infection and disease.
There is every reason to expect that SARS-
CoV-2 behaves similarly, and that
transmission via airborne micro-droplets is an
important pathway. Viral-RNA associated
with droplets <5 μm has been detected in air
14. International Invention of Scientific Journal Vol 05, Issue 01 Jan - 2021 Page | 14
, and the virus has been shown to maintain
infectivity in droplets of this size . Other
viruses have been shown to survive equally
well, if not better, in aerosols compared to
droplets on a surface . The current guidance
from numerous international and national-
bodies focuses on hand-washing, maintaining
social- distancing, and droplet precautions.
Most public health -organizations, including
the WHO, do not recognize airborne-
transmission except for aerosol-generating
procedures performed in healthcare settings.
Hand-washing and social- distancing are
appropriate but, in our view, insufficient to
provide protection from virus-carrying
respiratory-micro-droplets released into the
air by infected people. This problem is
especially acute in indoor or enclosed-
environments, particularly those that are
crowded and have inadequate ventilation
relative to the number of occupants and
extended exposure periods. airborne
transmission appears to be the only plausible
explanation for several super-spreading
events investigated that occurred under such
conditions , and others where recommended
precautions related to direct -droplet
transmissions were followed.” (5)
Materials and methods
Whit an observational point of view some
relevant literature are analyzed and after
producing an experimental hypothesis a
global conclusion is submitted to the
researcher .All literature comes from
biomedical databases (Pubmed and other
opens journal) Some Preprint are included in
reference due to the need to have rapid
scientific data in actual second wave of
Covid-19 pandemia.
Results
From literature :
Figure 8 SARS-CoV-2 clusters in mucin and deposition on airborne PM pollutants.
15. International Invention of Scientific Journal Vol 05, Issue 01 Jan - 2021 Page | 15
Front. Immunol., 24 September 2020 | https://doi.org/10.3389/fimmu.2020.579352
Airborne Particulate Matter and SARS-CoV-2 Partnership: Virus Hitchhiking, Stabilization and
Immune Cell Targeting — A Hypothesis
Z. ShadiFarhangrazi, Giulio Sancini, A. Christy Hunter and SeyedMoeinMoghimi(5)
Fig. 9
Tatiana Borisova et al :
“ interaction of SARS-CoV-2 envelope with
PM is possible in water surrounding. After
drying, PM can serve as a carrier for
transmission of SARS-CoV-2 immobilized at
their surface. PM and SARS-CoV-2 per se can
enter human- organism during nasal-
inhalation, and they both use the same nose-
to-brain delivery path-ways moving along
axons directly to the brain, influencing the
nervous system and exocytosis/endocytosis
in nerve- cells. Thus, PM can aggravate
neurological- symptoms of SARS-CoV-2 and
vice versa, due to their identical nose-to-
brain delivery mechanism and possible
interference of neuronal- effects. different
types of PM because of their ability to
interact with the plasma -membranes of
nerve cells can facilitate unspecific SARS-CoV-
2 entrance to the cells, and can influence
envelope features of SARS-CoV-2. Detailed
studies are required to analyze interaction of
SARS-CoV-2 withPM.”(7)
Nguyen Thanh Tung et al :-
“viable avian influenza viral -RNA was found
in PM up to 60 m downwind of commercial
turkey farms using reverse-transcription (RT)-
PCR and culture techniques . Influenza viral-
RNA was detected in air samples collected
approximately 2 km from the farms .
Aresearch study in the US reported that
PM10 had higher estimated concentrations of
avian influenza virus than PM2.5, but
16. International Invention of Scientific Journal Vol 05, Issue 01 Jan - 2021 Page | 16
PM2.5 may be further aerially transported .
That work also reported transmission of the
avian influenza- virus via PM2.5 within a state
and between states . Viruses may be
adsorbed through coagulation onto PM and
remain airborne for hours or days , thereby
increasing inhaled concentrations of virus via
PM in the lungs. PM2.5 may provide a good
platform to “shade” and “carry” the SARS-
CoV-2 during atmospheric- transport. PM
containing SARS-CoV-2 could be a direct-
transmission model in a highly polluted
area.”(8)
Luigi Sanità di Toppi et al
“One notable feature of all particulates PM is
that they can convey (and release) toxic
molecules and/or micro-organisms and/or
spores and/or viral particles. These
components can be absorbed or adsorbed by
the particulate particles, depending on
whether they enter them (where they are
potentially solubilized), or whether they bind
to the external -surface. the particles can be
broken, thus multiplying their polluting and
carrying- power. Particulate matter,
especially fine/ultrafine/ nano particles, can
enter the bronchi and the lung alveoli as well
as the blood (both plasma and erythrocytes),
the coronary- arteries, the heart, the
lymphatic system, and, ultimately, almost all
organs, with serious or very serious
consequences for health ( carcinogenic
and/or teratogenic- effects).
PM of various sizes can penetrate the
respiratory -tract, in some cases up to the
pulmonary alveoli. . The particulate matter of
various -sizes can penetrate the respiratory-
tract, in some cases up to the Pulmonary-
alveoli.”(9)
Fig. 10
Nikolai Nikitin et al :
“The human- infectious dose of the influenza- A
virus, when administered by aerosol to subjects
free of serum neutralizing antibodies, ranges
between 1.95 × 103
and 3.0 × 103
viral- particles.
To determine the concentration of virus -particles
in the air, the RT-PCR method is often used.
However, RT-PCR analysis provides information
on the total number of viral- particles, but not on
the number of infectious particles. Influenza-
virus genomic segments are chosen and packaged
at random, whereby only parts of the virions are
infectious.
According to various scientific- publications, data
about the influence of the virus subtype on the
effectiveness of influenza- transmission are
contradictory. The subtype-specific differences in
influenza virus- transmission were observed in
17. International Invention of Scientific Journal Vol 05, Issue 01 Jan - 2021 Page | 17
animal- models, and recipient animals did not
exhibit a preexisting influenza -virus specific
immune response. the pathogenicity of a virus -
subtype depends on the immune- status of the
recipients (human). The second point is (when)
how recently viruses of the same subtype
circulated in the population previously. In
research studies conducted by Alford et al ,
volunteers were exposed to carefully titrated
aerosolized influenza- virus suspensions by
inhaling through a face- mask. The
demonstration of infection in participants of
the study was achieved by recovery of
infectious viruses from throat swabs, taken
daily, or by sero-conversion, that is, the
development of neutralizing- antibodies.
The use of carefully titrated viral- stocks
enabled the determination of the minimal -
infectious dose by aerosol -inoculation. The
approximate 50% human- infectious dose
(HID50-50% human infectious dose) of virus
per volunteer was from 1 to 126 TCID50 (the
tissue culture 50% infectious dose). The
dose for half of the volunteers was 5 TCID50.
The other half of the men, who had very low
or non-detectable pre-inoculation antibody
titers, were infected with 0.6 to 3 TCID50. The
study reliably shows that the human -
infectious dose of the influenza- A virus,
when administered by aerosol to subjects
free of serum neutralizing antibodies, is
approximately 3 TCID50. The approaches used
in this study allow the precise number of
infectious- particles in the total number of
particles to be determined.Ward, with co-
workers , confirmed experimentally that three
log10 copies/mL corresponded to
1 TCID50/mL. That is, one TCID50/mL
contains 1000 copies of the viral-
genome.According to other reports, the
aerosol infection dose for humans was about
1.95 × 103
viral genome copies, for
approximately 300–650 copies of human-
influenza viruses were contained in 1
TCID50, according to previous studies . During
the 2009-2010 influenza- season (from
December to April), Yang, with coworkers ,
collected samples from a health centre, a day-
care centre, and airplanes. The
concentrations of airborne influenza- viruses
(A/PR/8/34 (H1N1) and
A/swine/Minnesota/1145/2007 (H3N2))
were measured. The influenza A virus -RNA
was quantified by RT-PCR. Fifty percent of
the samples collected contained the influenza
A -virus, with concentrations ranging from
5.8 × 103
to 3.7 × 104
genome copies per m3
.
The average concentration of the virus was
1.6 ± 0.9 × 104
genome copies per m3
,
corresponding to 35.4 ± 21.0 TCID50 per
18. International Invention of Scientific Journal Vol 05, Issue 01 Jan - 2021 Page | 18
m3
air. According to Yang et al. , 1 TCID50 of
A/PR/8/34 (H1N1) stock was equivalent to
2.1 × 103
genome- copies, and the ratio for
the pandemic A/California/04/2009 (H1N1)
strain was determined to be 452 ± 84
genome copies per TCID50.
Using the measured airborne -virus
concentration and an adult breathing- rate,
Yang, with colleagues , estimated the
inhalation doses during exposures of 1 h (e.g.,
the duration of a clinical visit), 8 h (a
workday), and 24 h to be 1.35 × 104
, 1.06 ×
105
, and 3.2 × 106
viral- particles (or 30 ± 18,
236 ± 140, and 708 ± 419 TCID50),
respectively. Compared with the aerosol
HID50 0.6–3 TCID50 , these doses are
adequate to induce infection. In other words,
over 1 h, the inhalation dose is estimated to
be 30 ± 18 TCID50 or about 16 000 particles
of the influenza A -virus, which is more than
enough to induce infection” (10)
Byung Uk Lee:
“Corona-virus-Bio-aerosols Artificially-
generated aerosols carrying corona-viruses
have been studied with testing their stability.
First, the Middle East -respiratory syndrome
corona-virus (MERS-CoV) was aerosolized
for 10 min and its viability was measured at
40% and 70% relative humidity (RH)
conditions. The results revealed that MERS-
CoV was stable at 40% RH. the virus
viability was significantly lost at 70% RH.
Second, SARS-CoV-2 was aerosolized for 3
hours and its viability was analyzed. It was
found that the virus was viable even after 3
hours, with limited loss of viability .Corona-
virus genetic- materials in the air have been
detected in several research studies. In a
study by Azhar et al. in Saudi- Arabia, the
MERS-CoV genome was detected in an air
sample from a camel barn of an infected
patient . In Wuhan (China) and Nebraska
(US), SARS-CoV-2 nucleic acid tests
conducted on air- samples gave positive
results at an intensive care- unit of a hospital
in Wuhan (China) and in a patient room of a
university medical center in Nebraska (US) .
In Florida (US), SARS-CoV-2 was detected in
air samples at the Student Health Care -
Center at the University of Floridavia RT–
PCR analysis . In this study in Florida, the
SARS-CoV-2 concentration was estimated to
be 0.87 virus genomes/L air. in a study by
Chia et al. in Singapore, SARS-CoV-2 was
detected in air samples from the airborne-
infection isolation rooms of infected patients
via RT–PCR analysis and ORF labassay . In a
study by Liu (2020), SARS-CoV-2 RNA was
detected in air samples from hospitals and
19. International Invention of Scientific Journal Vol 05, Issue 01 Jan - 2021 Page | 19
public areas, such as department stores, in
Wuhan (China) . The detection of corona-
virus genes in air samples implies that it is
highly probable that corona-virus bio-
aerosols were present at the sampling -
locations. In a study by Lednickyet al. , the
isolation of viable SARS-CoV-2 from air-
samples of the surroundings (2 to 4.8 m
away) of patients in a hospital was reported
in Florida (US) .(11)
Estimated minimum. size of particles
(assuming homogenous distribution of
viruses in released respiratory fluid particles
and virus size of 0.09 μm) potentially carrying
SARS-CoV-2 and corresponding aerosol
suspension times.
Fig. 11
Michael Riediker et al :
“The mean estimated viral- load in micro-
droplets emitted by simulated- individuals
while breathing- regularly was 0.0000049
copies/cm3 , with a range of 0.0000000049
to 0.637 copies/ cm3 . The corresponding
estimates for simulated coughing individuals
were a mean of 0.277 copies/cm3 per cough,
with a range of 0.000277 to 36 030
20. International Invention of Scientific Journal Vol 05, Issue 01 Jan - 2021 Page | 20
copies/cm3 per cough. The estimated
concentrations in a room with an individual
who was coughing- frequently were very
high, with a maximum of 7.44 million
copies/m3 from an individual who was a high
emitter. regular breathing from an individual
who was a high emitter was modeled to
result in lower- room concentrations of up to
1248 copies/m3 . In this modeling research
study, breathing and coughing were
estimated to release large numbers of
viruses, ranging from thousands to millions of
virus- copies per cubic meter in a room with
an individual with COVID-19 with a high viral -
load, depending on ventilation and micro-
droplet formation process. The estimated
infectious- risk posed by a person with typical
viral load who breathes normally was low.
The results suggest that only few people with
very high viral- load pose an infection risk in
poorly ventilated closed -environments.
These findings suggest that strict respiratory
protection may be needed when there is a
chance to be in the same small- room with an
individual, whether symptomatic or not,
especially for a prolonged- period” (12)
Marcelo I. Guzman :
“Various deposition mechanisms can exist,
including inertial impaction, gravitational
settling, Brownian -motion, turbulent
deposition, interception, and electrostatic
attraction . The smallest- particles (8 μm) are
size dependently deposited from the nasal-
passage to the bronchioles. Multiple factors, (
age, weight, sex, physical activity level, and
disease state) impact respiration and
deposition- profiles . Larger- particles can be
inhaled into the respiratory tract under
exertion breathing because the oral cavity is
larger and results in bypassing of the nasal-
cavity filtration mechanism“.(13)
Silvia Comunian et al :
“The avian influenza -virus (H5N1) could be
transported across long distances by fine
dust during Asian- storms , and the
correlation between PM concentration and
the virus spread has been observed in the
case of the spread of measles in China.
PM2.5 concentrations in 21 Chinese -cities
and the number of measles cases per day per
city were studied. The analysis showed a
positive correlation between those 2 factors.
The 10 μg/m3 increase in PM2.5 per day is
associated with a significant rise in the
disease-incidence . A similar analysis of the
children’s respiratory syncytial -virus (RSV)
spread in China in 2015 shows the same
correlation. RSV is a virus that causes damage
to the lungs and bronchitis. A positive -
correlation between the virus and PM-
concentration was observed. In fact,
pollution increases the risk of RSV-infection .
A 2018 analysis, carried out in the Po- Valley,
associates hospitalizations and the number of
new RSV cases with PM10- concentration.
The data for the analysis were collected by
ARPA- (Regional Environmental Protection-
Agency) in the region. The results of this
analysis showed that, in the designated
period, the highest number of
hospitalizations occurred in Milan, the city
that had reached the maximum
concentration of PM10. This study also shows
a correlation between short- and medium-
term PM10- exposures ( in the two weeks
preceding hospital admission) and increased
risk of hospitalization owing to RSV-
bronchiolitis among infants . There are
several mechanisms by which PM induces an
21. International Invention of Scientific Journal Vol 05, Issue 01 Jan - 2021 Page | 21
increase in infected- cases. A mechanism can
be that the virus is bound to particles and
transported, if favored by climatic-
conditions.”(14).
Tatiana Borisova et al :
“CoVs are large enveloped non-segmented
positive-sense RNA viruses. Viral- envelopes
consist of proteins and lipid components, and
enveloped -viruses require the fusion of their
lipid- envelope with the host cell membrane
to entry the infected-cells .
interaction of SARS-CoV-2 envelope with PM
is possible in water -surrounding. After
drying, PM can serve as a carrier for
transmission of SARS-CoV-2 immobilized at
their surface. In this research study, we have
suggested that lipid- constituents of the viral
envelope can be very important for
unspecific interaction of viral particles with
different surfaces, including air -pollution
particulate matter- (PM). It should be
emphasized that this interaction capability
can be inherent mainly to enveloped viruses.
it was confirmed that air- pollution PM can
travel across border for a long -distance and
inhalation with fine and ultrafine- PM (the
aerodynamic diameter is less than 2.5 μm
and 0.1 μm, respectively) is associated with
many diseases, including neurological ones .
The effect of fine dust -concentrations in the
air in the Republic of Korea (2016–17) on the
incidence of viral respiratory- infections
caused by the human -corona-virus,
respiratory syncytial virus, human
metapneumovirus, adenovirus, rhinovirus,
human bocavirus, human parainfluenza virus,
and influenza- virus was investigated. It was
concluded that when the weekly average
concentration of fine dust increased, the
incidence of infections by the human-corona-
virus, human meta-pneumo virus,
adenovirus, human boca virus, human para
influenza virus, and influenza also increased .
In the USA, the majority of the positive cases
of highly pathogenic avian influenza (HPAI)
H5N2 might have received airborne- virus
carried by fine air pollution PM, and these
results provide insights into the risk of
airborne transmission of HPAI virus via fine
dust- particles. In Beijing, China, association
between daily PM2.5 (PM with size lesser
than 2.5 μm) and influenza-like illness ILI risk
was investigated using a generalized additive
model. A strong positive- relationship
between PM2.5 and ILI risk at the flu season
was established, but the effect of PM2.5
differed across age groups . “(15)
SimaAsadi et al :
“These results show that dried influenza-
virus remains viable in the environment, on
materials like paper tissues and on the bodies
of living- animals, long enough to be
aerosolized on non-respiratory -dust particles
that can transmit infection through the air to
new mammalian- hosts. “(15)
22. Page | 22
Fig. 12 Schematic for aerodynamic particle -
sizer (APS) experiments to quantify the
airborne particulates generated by awake,
un-restrained (mobile) guinea- pigs (GP)
(Supplementary Fig. 1). b Representative
instantaneous particle emission- rate (left
axis) and instantaneous guinea pig
movement velocity (right axis) vs. time for a
mobile guinea -pig in a cage with granular
dried corncob (CC) bedding. c Time-averaged
particle emission- rate over
1 min (N¯(1))(N¯(1)) vs. time-averaged guinea
pig movement- velocity over
1 min (V¯(1))(V¯(1)). Solid line is the power
law fit with exponent 0.93, correlation-
coefficient 0.80, and p-value
9.6 × 10−15. d Schematic for APS experiments
to measure the particulates produced by
anesthetized or euthanized (stationary)
guinea pigs . e Particle emission rates, time-
averaged over 15 min (N¯(15))(N¯(15)), for
three mobile guinea pigs (GP1, GP2, and
GP3). Gray markers denote background
particle counts without a guinea pig in the
cage with different beddings (dried corncob
granulas (CC), polar fleece-covered absorbent
pads (PF), or no bedding (No) on the plastic-
cage floor).
23. International Invention of Scientific Journal Vol 05, Issue 01 Jan - 2021 Page | 23
f Measurements of the particle emission-
rates, time-averaged over
15 min (N¯(15))(N¯(15)), for stationary guinea
-pigs, performed prior to inoculation (day 0)
and on days 1, 2, and 3 post-inoculation with
influenza A/Panama/2007/1999 (H3N2)
(Pan99) virus, and after euthanasia.
Horizontal- gray dashed line denotes
background particle counts of empty -cage.
Particle emission rates are the total of all
particles detected in the size range of 0.3–20
μm in diameter .
Science News
from research organizations
Research exposes new vulnerability for SARS-
CoV-2
Electrostatic interactions enhance the spike
protein's bond to host cells
August 11, 2020 Northwestern University
“The spike- protein contains the virus'
binding- site, which adheres to host cells and
enables the virus to enter and infect the
body. Using nanometer-level simulations, the
researchers discovered a positively- charged
site (known as the polybasic cleavage- site)
located 10 nanometers from the actual
binding site on the spike- protein. The
positively charged site allows strong bonding
between the virus protein and the negatively
-charged human-cell receptors.
Leveraging this discovery, the researchers
designed a negatively -charged molecule to
bind to the positively- charged cleavage site.
Blocking this site inhibits the virus from
bonding to the host cell.”(17)
Sandhya Verma et al :
“The corona-virus-nucleocapsid (N) protein is
a multifunctional viral- gene product that
encapsidates the RNA genome and also plays
some as yet not fully defined role in viral -
RNA replication and/or transcription. A
number of conserved negatively charged
amino- acids are located within domain III in
the carboxy- end of all corona-virus N
proteins. Previous studies suggested that the
negatively- charged residues are involved in
virus assembly by mediating interaction
between the membrane (M) protein carboxy
-tail and nucleocapsids Corona-virus N
proteins are phosphorylated. The proteins
are highly- basic, with isoelectric- points (pI)
of 10.3 to 10.7 . A three-domain structure for
the protein has been proposed based on
early sequence comparisons of MHV strains .
The amino -terminal and central domains of
all corona-virus N proteins exhibit an overall
positive charge, whereas the carboxy-
terminal domain is acidic. Conservation of
negatively- charged amino acids within the
carboxy ends of all corona-virus N proteins
suggests that the residues are functionally
relevant. data from an earlier study suggest
that the carboxyl- end of the protein
mediates interaction with the M protein
during assembly, and the charged resides
within the region were hypothesized to
possibly facilitate the interaction .
Within the carboxy-terminal 22 amino-
acids of the MHV-CoV N protein there
are eight negatively -charged residues
.” (18)
24. International Invention of Scientific Journal Vol 05, Issue 01 Jan - 2021 Page | 24
Figure n 13 Illustration of the rationale of
the electrical measurement for virus titer
measurement and classification.
(a) Virions- distributions inside the coaxial -
resonator in the absence of electric field. (b)
Polarized-- virions when an electrostatic field
is applied. A coaxial resonator has an inner
conductor (the +ve electrode, where positive
charges accumulate, +Q) surrounded by a
hollow space that is surrounded by a
conducting shield (−ve electrode, where the
negative- charges accumulate, −Q). (c)
Schematic of the polarized -virus particles
inside an alternating current electric
field.From Virus -detection and quantification
using electrical -parametersMahmoud Al
Ahmad et al .
Edris Joonaki et al :
“Corona-virus- genomes are comparatively
large for RNA viruses and severe acute
respiratory syndrome corona-virus 2 (SARS-
CoV-2) encodes an extensive complement of
non-structural- proteins ( 3-chymo-trypsin-
like [3CL] protease, papain-like protease,
etc.) as well as structural proteins as follows:
spike (S) glycoprotein, envelope (E)
glycoprotein, membrane (M) glycoprotein,
and nucleocapsid (N) phosphoprotein.1 The
SARS-CoV-2 spike (S) glycoprotein exhibits
76% amino -acid sequence identity with the
SARSCoV S (Urbani strain) and 80% identity
with S proteins of bat SARSr-CoV ZXC21 and
ZC45.
1,2 CoV S- glycoproteins form club-shaped
trimers and decorate the viral membrane,3
giving corona-virus- virions their
characteristic morphology. As a substantial
component of the outer surface of the virion,
S likely plays a critical role in adsorption of
viruses onto the solid- surfaces under various
environmental conditions.
For further clarification, Figure 14A depicts a
central -slice through an electron micrograph
of mouse hepatitis- virus, which exhibits the
presence of S on the virion- surface.
Viruses adsorb to surfaces through 2 main
mechanisms, van der Waals (mainly mineral
surfaces and, more importantly,
25. International Invention of Scientific Journal Vol 05, Issue 01 Jan - 2021 Page | 25
electrostatic- interactions (charged surfaces
in the presence of ions and or not neutral
pH11–13). These 2 forces dictate the
adsorption of viruses to surfaces. Although
the interplay between these 2 forces is
difficult to separate, indications of the
interactions can be determined from past
data. Viruses tend to be more hydro-phobic
than proteins, thus they are attracted to
metal- surfaces because of mainly van der
Waals interactions as well as hydro-phobic
effects. their ability to maintain the virus’s
viability and allow it to remain infectious is
more of a function of the humidity and
temperature, thus the surface energy of the
water molecules plays a large- role in the
interaction between a virus- particle and a
surface.
SARS-CoV-2 virions can be adsorbed onto
metal- surfaces ( gold and stainless -steel) in
addition to hydroxyl functional group- and
oxygen-containing substrates ( wood, cotton,
paper, and glass) depending on the surface
chemistry and environmental -conditions (
bulk fluid pH, surface charge, temperature,
etc).Hydrogen bonding plays a key role in the
adsorption of viruses to the hydroxyl-
containing surfaces and in the presence of an
aqueous phase thin film- layer. The strength
of the bond to the surface would be high in
the presence of –O–H - - -O bonding,
particularly in pH environments where the
carboxylic acid on the virus is de-protonated
(typically above a pH of 4). At neutral pH,
most viral particles have a net negative
charge because they have an isoelectric point
below 7. However, due to the large- size of
virus particles and their large variety of
surface- proteins, there are still multiple
patches of positive and negative charge in
the pH range where viruses are stable
(typically from pH 5–8). NH2, NH3 +, COOH,
andCOOgroups of amino acids in the SARS-
CoV2 S protein drive adsorption onto the
solid surfaces through double electrostatic-
interactions between the virion’s ionized
surface-active species and the oppositely-
charged surfaces, as well as hydrogen
bonding based on the surface characteristics.
at neutral pH- values, the negatively -charged
virus particles would be adsorbed
significantly less on a stainless-steel surface
because of electrostatic- repulsion, given that
both virion and substrate surface have
negative- charges. With augmentation of the
cation- concentration, however, the
repulsion would be decreased, and the
quantity of adsorbed -viruses would increase.
Figure 14 presents the potential molecular-
interactions between the SARS-CoV-2 viral
proteins and solid surfaces at different pH
values and fluid chemistries. As denoted in
Figure 14 A, at pH values below the
isoelectric- point, the overall charge of SARS-
CoV-2 could be positive, given that both the
carboxylate and amine groups on the outer
surface are protonated, and hydrogen
bonding would be formed to hydroxyl-
containing surfaces suchas wood, cotton, or
paper. At pH environments above the
isoelectric-point , the outer surface of virions
would be deprotonated and therefore
negatively- charged and cannot be adsorbed
on the surface with the same charge.
Accordingly, lower virus -adsorption onto the
surfaces would occur at higher pH values.
Instead, they can interact strongly with
divalent and/or monovalent- cations if they
existed in a brine electrolyte solution (more
details are presented in the following
section). The charge and counterions from
the electrolyte could lead to thinner double-
26. International Invention of Scientific Journal Vol 05, Issue 01 Jan - 2021 Page | 26
layer and lower- repulsion forces, and again
hydrogen bonding formed to surface
hydroxyl groups, which results in promoting
the virus- adsorption process. The gold-
surface of an electrode in the quartz crystal -
microbalance (QCM) biosensor, which works
on the basis of the oscillating frequency
alteration could be employed for monitoring
of the virus -surface adsorption and
desorption phenomena with or without the
presence of negatively- charged surface
active species in the liquid- phase”. (20)
Fig n. 14 Molecular- Interactions at SARS-
CoV-2 Viral Interfaces in Different
Environmental Conditions- Model of the
potential molecular interactions among
viruses and between virus and different solid-
surfaces having negative -surface charge
and/or hydroxyl functional groups at (A)
relatively low pH environment, below the
isoelectric -point; (B) relatively high pH-
condition, above isoelectric point in presence
of external ions (salts); and (C) way below the
isoelectric point in the presence of potential
chemistries (for removal from surface
purposes) with negative- surface charge
Energy landscape theory of SARS-CoV-2
complexes with Particulate Matter. Gianluigi
Zangari del Balzo This manuscript was
compiled on June 24, 2020
Preprinteresearchgate
“The presence of multiple non-covalent
interactions between SARS-CoV-2 and PMs is
a source of cooperativity between them.
Only when these interactions cooperate is a
stable 235 single conformation produced,
that of the complex [SARS-CoV- 2]⊕[PM].
The complex created is therefore much
stronger than might be expected from the
sum of their individual- strengths. This can
explain the rapid spread of the pandemic in
the areas of the greatest- pollution. This
exceptional cooperative optimization can
also explain the severity and difficulty of
treating the forms of interstitial pneumonia
that occur in Italy and worldwide. But not
only that, it could perhaps also help us
understand the origin and initial mutations
of SARS-CoV-2” (22)
27. International Invention of Scientific Journal Vol 05, Issue 01 Jan - 2021 Page | 27
Fig. 15 Energy rugged funnel landscape for a realistic [SARS-CoV-2]⊕[PMs] complex formation
28. International Invention of Scientific Journal Vol 05, Issue 01 Jan - 2021 Page | 28
Experimental projecthypotesys
In order to verify the effect of electrical
charge on virus surface and the entity of
ligand force between virus and carriers ( in
airborne condition ) it is possible to think to 2
closed environment with virus in aeresols or
with carrier like
particulate matter :
1 ) environment under standard condition
2) environament uder with modified
condition ( differente level
low,moderate,high ) : electrical charge
influence, wind flux
Chemical phisic properties ( pressure
temperature) humidity and other that can be
applied. After significative time exposure this
aeresols must to be tested to verify the PM
particle viral binding whit specific nalitical
method. The PM must to be separated from
the aereosol according their SIZE a Different
data in the 2 enviroment show the influence
on weak links on virus interaction with PM.
Discussion
It is interesting that in airborne transmission
the smaller particles are more dangerous
then the larger ones: The smaller particles
can introduce better in deep lungs zones
since alveoli . See behavior of PM 2,5 vs PM
10. Chemical physical properties of virus
surface are involved in the link binding whit
carriers like PM. This link is also responsible
in the severity of disease . The composition
and properties of virus surface are directly
linked with airborne Pattern. WHO officially
recognized direct contact and by droplet
primary way of corona-virustransmission
while airborne less involved. But related the
preventive measure adopted the great
number of death worldwide are clearly
explained?
Conclusion
Some parametere are relevant in evaluating
airborne profile of some respiratory virus
transmission. Many parameteres are involved
but also need to be deeply studied are : Virus
size, evelope properties , electrical charge,
chemical -physic intereaction between virus
and carrieres , attractive forces and
repulsion, weak binds . this properties must
to be considered in adoption of preventive
measure . Not only medicine and biology but
also chemistry and physic science can help in
better explain some characteristic of
respiratory virus, the law that regulate their
diffusion and permanence time in aereols or
linked with carrier. Chemical composition of
virus envelope, kind of chemical links with
carriers, Electrical charge, repulsion forces ,
and environmental factors influence airborne
characteristic of some respiratory virus as
well as time of permanence in aeresols. H-
bindings, van der waals interations,
hydrophobic , electrostatic interactions are
common weak link between virus particles
and carriers.
It is to conclude that is not possible control a
corona-virus pandemia without not consider
this last factors. The same severity of disease
is related to the chemical -phisical link
between virus and PM and the therapeutic
strategy also .
Conflict of interests : no
Ethical consideration
29. International Invention of Scientific Journal Vol 05, Issue 01 Jan - 2021 Page | 29
this work is produced under all international
ethical rules
That can be applied.
Clarification
This work is produced without any diagnostic
or therapeutic intent only to produce
research hypotesys to be submitted to other
researcher.
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