BI 101 Homework 5
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DNA plays four key roles in cells: (1) it carries the genetic instructions for making proteins, (2) it is the blueprint for RNA, (3) it is the template for DNA replication, and (4) it undergoes transcription to make messenger RNA. These roles are enabled by DNA's double-helix structure which allows it to stably store and replicate genetic information. The document provides a homework assignment on DNA that involves explaining DNA's roles and structure, matching terms to descriptions, comparing messenger RNA, transfer RNA and ribosomal RNA, transcribing a DNA sequence, translating an mRNA sequence, and completing a table after reviewing pages from the textbook and watching an animation on transcription and translation.
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instructions based on those by KD Blographics) DNA and RNA - Compounds and Structure:
DNA the genetic material found in the nucleus of eukaryotic cells, is a very complex molocule
formed using six compounds, PHOSPHATE, a SUGAR (deoxyribose), and four BASES
(adenine, guanine, cylosine, and trymine), Lkewise, the complex RNA molecule found in cells is
formed using six compounds, PHOSPHATE, a SUGAR (ribose), and four BASES (adenine,
guanine, cytosine, and uracil). Foliow the instructions found with the DNA puzzie kit to
categorize puzzfe pieces, and ibontify the specific compound name for each puzzle piece labeled
1 - 8 . You will not be able to fill out the entire chart at once, but list the name of each pioce
when you can identily it and whether it is found in DNA, RNA, or BOTH. a. Write the inltial of
the bases in the order found in your DNA ladder, below. DNA iop strand: C G A A I G A S Q.
Briefly describe the pairing of bases in the "rungs" of your DNA ladder. Is there a pattern to the
base pairing (use base names), and is there a pattern to the base types (purines and pyrimldines)
found in the pairs? Why do you think they pair up in this manner? Kes) Guantie and Cu Mosine,
Adiec and Thamire. DNA REPLICATION: Before cells car divide inso new cells, they must
make components for the new cels. Duplicating DNA called DNA replication, is part of thil
process. Foliow DNA wit instructions to make new DNA using the original double strand, then
answer the following. Q. Describe the role hydrogen bonding plays in the process of replication,
and the compesition of the resulting DNA strands including whether or not they are identical
using key words like "original" and "eopy". Higlvogen ponding heivs to kec an ins shuchere of
plda stroid TRANSCRIPTION - DNA to RNA: Although DNA carries the code to make
proteins, it cannot leave the nucleus and travel to the ribosomes where protein synthesis occurs.
Something else must take the message. Follow the instructions with your DNA kit to explore
transcription, and then answer the questions, below. Describe the base sequence of the mRNA
you have formed showing which bases pair up with the bases of the oriainal DNA. Briefly
describe how the results of transcription differ from the process of DNA replication.
Tronsciption differs from DNp repicatiop, making RNA COpy TRANSLATION - RNA to
PROTEINS: The genetic code is carried in linear sequence of bases. A protein is made of a linear
sequence of amino acids. The languages are different, but if translated, the codes are the same.
Follow the instructions in your DNA kit to study translation. When you've completed the puzzle
activity, fill in the answers, below. Fill in each three bese sequence in the mRNA and the tRNA
that you used in this lab activily. Look at the table of amino acids on the last page. Each column
represents the base sequences from DNA. mRNA and TRNA that transiate to an .
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B10 113 Spring 2023 2. In prokaryotes, sometimes a single gene can be transcribed by multiple RNA Polymerases at the same time. Likewise, a single mRNA may be translated by multiple ribosomes at the same time. A cartoon representing this scenario is shown below. A piece of prokaryotic chromosomal DNA containing a single gene is illustrated as a thick line. Thin lines radiating from the DNA are mRNA molecules in the process of being transcribed. Ribosomes shown are attached to the mRNAs. RNA polymerases and newly synthesized proteins are not shown. Different positions in the diagram are labeled with letters. a. Draw in a circle representing each RNA polymerase that is in the process of transcribing the gene into mRNA. Label each circle "RNA polymerase." b. Recall that RNA polymerase can only add nucleotides to the 3 ' end of a growing RNA molecule. Label the 3 end and 5 ends of each mRNA that is being transcribed. (Hint: the 3 'end will be next to the RNA polymerases you drew in part a). c. Look at the RNA transcript labeled D-E. Is the 5 end of the RNA closer to position D or position E? Explain your answer. d. Imagine there is one RNA polymerase that has just started transcription near the promoter. There a different RNA polymerase that is almost done with transcription near the terminator. Which RN polymerase will have synthesized (made) a longer RNA molecule? Circle one: the RNA polymerase near the promoter the RNA polymerase near the terminator 10113 Spring 2023 Problem Set 4 e. Is the promoter closer to position A or position F? Explain your answer. f. Is the RNA polymerase located at position J moving to the right or the left? g. Imagine each RNA polymerase has finished making each mRNA in the diagram. Will each mRNA have the same length and sequence? Explain your answer. h. Look at the ribosome labeled C . Is it moving closer to or further from position L? Explain your answer. i. Which ribosome has made more peptide bonds: ribosome G or ribosome H ? j. Could the scenario happening in this diagram occur in eukaryotes? Explain why or why not.
.
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1. Report on Gene and Related Protein (3-4 pages) (30 pts) (details on navigation of PubMed site in separate Power Point)
a. The name and gene code for your gene product/protein.
b. Determine chromosome location of your gene. Paste a picture of the chromosome from MAP VIEWER into your report and describe.
c. Identify the size of the coding region of your gene, include a link to the GENE page, and explain what the coding region is. Note: your gene sequence may be very long, do not cut and paste the sequence into your report.
d. Determine the intron /exon structure of your gene including the number of introns and exons. Cut and paste a schematic diagram of your gene into the report, indicate position of introns, and exons, and provide a caption for the figure that describes how the sequence of the primary mRNA transcript is related to the gene (DNA) sequence.
e. Identify the sequence of the mature mRNA and copy this sequence into your report with the appropriate link to this sequence. (Note: the mRNA sequence will actually be shown as a “complementary DNA” or “cDNA.” The sequence given in the database is an exact copy of the mRNA sequence, except that it has T instead of U. ) Explain how this sequence is derived from the sequence of the coding region identified above.
f. Identify the Open reading frame in the mature mRNA (Coding sequence, CDS) Find the START codon, STOP codon, and highlight the open reading frame. Explain where translation begins and ends.
g. Translate the first ten amino acids of the Open Reading Frame.
h. Identify the protein sequence, and copy this sequence into your report with a link to the appropriate page. Check the first ten amino acids of the database protein sequence against the ten amino acids you translated above.
i. Compare your (human) gene sequence with the same gene sequence from other species. Copy a representative portion of the sequence alignment into your report that shows the similarities/differences between the human protein sequence and the homologous protein sequence from at least one other species. (Note: sequences will align if you use the Courier font). Explain how the similarity/difference in sequences explains the similarities and differences between species.
j. Paste a picture of the secondary (worm view) and tertiary (space filling) structures of your protein into your report. Note: structures have not been determined for all proteins, so you may have been given an identifier for a similar protein. Describe things about the structure, for example features of the shape, charge, or hydrophobicity.
Genome database assignment and stepwise instructions
Goal: improve student understanding of relationship between genes, protein(gene products), and biological functions.
The exercise:
Each student will be assigned a gene product/protein that will be di ...
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instructions based on those by KD Blographics) DNA and RNA - Compounds and Structure:
DNA the genetic material found in the nucleus of eukaryotic cells, is a very complex molocule
formed using six compounds, PHOSPHATE, a SUGAR (deoxyribose), and four BASES
(adenine, guanine, cylosine, and trymine), Lkewise, the complex RNA molecule found in cells is
formed using six compounds, PHOSPHATE, a SUGAR (ribose), and four BASES (adenine,
guanine, cytosine, and uracil). Foliow the instructions found with the DNA puzzie kit to
categorize puzzfe pieces, and ibontify the specific compound name for each puzzle piece labeled
1 - 8 . You will not be able to fill out the entire chart at once, but list the name of each pioce
when you can identily it and whether it is found in DNA, RNA, or BOTH. a. Write the inltial of
the bases in the order found in your DNA ladder, below. DNA iop strand: C G A A I G A S Q.
Briefly describe the pairing of bases in the "rungs" of your DNA ladder. Is there a pattern to the
base pairing (use base names), and is there a pattern to the base types (purines and pyrimldines)
found in the pairs? Why do you think they pair up in this manner? Kes) Guantie and Cu Mosine,
Adiec and Thamire. DNA REPLICATION: Before cells car divide inso new cells, they must
make components for the new cels. Duplicating DNA called DNA replication, is part of thil
process. Foliow DNA wit instructions to make new DNA using the original double strand, then
answer the following. Q. Describe the role hydrogen bonding plays in the process of replication,
and the compesition of the resulting DNA strands including whether or not they are identical
using key words like "original" and "eopy". Higlvogen ponding heivs to kec an ins shuchere of
plda stroid TRANSCRIPTION - DNA to RNA: Although DNA carries the code to make
proteins, it cannot leave the nucleus and travel to the ribosomes where protein synthesis occurs.
Something else must take the message. Follow the instructions with your DNA kit to explore
transcription, and then answer the questions, below. Describe the base sequence of the mRNA
you have formed showing which bases pair up with the bases of the oriainal DNA. Briefly
describe how the results of transcription differ from the process of DNA replication.
Tronsciption differs from DNp repicatiop, making RNA COpy TRANSLATION - RNA to
PROTEINS: The genetic code is carried in linear sequence of bases. A protein is made of a linear
sequence of amino acids. The languages are different, but if translated, the codes are the same.
Follow the instructions in your DNA kit to study translation. When you've completed the puzzle
activity, fill in the answers, below. Fill in each three bese sequence in the mRNA and the tRNA
that you used in this lab activily. Look at the table of amino acids on the last page. Each column
represents the base sequences from DNA. mRNA and TRNA that transiate to an .
B10 113 Spring 2023 2- In prokaryotes- sometimes a single gene can be.docx
B10 113 Spring 2023 2. In prokaryotes, sometimes a single gene can be transcribed by multiple RNA Polymerases at the same time. Likewise, a single mRNA may be translated by multiple ribosomes at the same time. A cartoon representing this scenario is shown below. A piece of prokaryotic chromosomal DNA containing a single gene is illustrated as a thick line. Thin lines radiating from the DNA are mRNA molecules in the process of being transcribed. Ribosomes shown are attached to the mRNAs. RNA polymerases and newly synthesized proteins are not shown. Different positions in the diagram are labeled with letters. a. Draw in a circle representing each RNA polymerase that is in the process of transcribing the gene into mRNA. Label each circle "RNA polymerase." b. Recall that RNA polymerase can only add nucleotides to the 3 ' end of a growing RNA molecule. Label the 3 end and 5 ends of each mRNA that is being transcribed. (Hint: the 3 'end will be next to the RNA polymerases you drew in part a). c. Look at the RNA transcript labeled D-E. Is the 5 end of the RNA closer to position D or position E? Explain your answer. d. Imagine there is one RNA polymerase that has just started transcription near the promoter. There a different RNA polymerase that is almost done with transcription near the terminator. Which RN polymerase will have synthesized (made) a longer RNA molecule? Circle one: the RNA polymerase near the promoter the RNA polymerase near the terminator 10113 Spring 2023 Problem Set 4 e. Is the promoter closer to position A or position F? Explain your answer. f. Is the RNA polymerase located at position J moving to the right or the left? g. Imagine each RNA polymerase has finished making each mRNA in the diagram. Will each mRNA have the same length and sequence? Explain your answer. h. Look at the ribosome labeled C . Is it moving closer to or further from position L? Explain your answer. i. Which ribosome has made more peptide bonds: ribosome G or ribosome H ? j. Could the scenario happening in this diagram occur in eukaryotes? Explain why or why not.
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Drug TanzeumDiseasesDiabetesGene and Gene Productglucago.docx
Drug Tanzeum
Diseases Diabetes
Gene and Gene Product glucagon-like peptide (GLP)-1 fusion protein
Gene ID GCG
Structure 3IOL
1. Report on Gene and Related Protein (3-4 pages) (30 pts) (details on navigation of PubMed site in separate Power Point)
a. The name and gene code for your gene product/protein.
b. Determine chromosome location of your gene. Paste a picture of the chromosome from MAP VIEWER into your report and describe.
c. Identify the size of the coding region of your gene, include a link to the GENE page, and explain what the coding region is. Note: your gene sequence may be very long, do not cut and paste the sequence into your report.
d. Determine the intron /exon structure of your gene including the number of introns and exons. Cut and paste a schematic diagram of your gene into the report, indicate position of introns, and exons, and provide a caption for the figure that describes how the sequence of the primary mRNA transcript is related to the gene (DNA) sequence.
e. Identify the sequence of the mature mRNA and copy this sequence into your report with the appropriate link to this sequence. (Note: the mRNA sequence will actually be shown as a “complementary DNA” or “cDNA.” The sequence given in the database is an exact copy of the mRNA sequence, except that it has T instead of U. ) Explain how this sequence is derived from the sequence of the coding region identified above.
f. Identify the Open reading frame in the mature mRNA (Coding sequence, CDS) Find the START codon, STOP codon, and highlight the open reading frame. Explain where translation begins and ends.
g. Translate the first ten amino acids of the Open Reading Frame.
h. Identify the protein sequence, and copy this sequence into your report with a link to the appropriate page. Check the first ten amino acids of the database protein sequence against the ten amino acids you translated above.
i. Compare your (human) gene sequence with the same gene sequence from other species. Copy a representative portion of the sequence alignment into your report that shows the similarities/differences between the human protein sequence and the homologous protein sequence from at least one other species. (Note: sequences will align if you use the Courier font). Explain how the similarity/difference in sequences explains the similarities and differences between species.
j. Paste a picture of the secondary (worm view) and tertiary (space filling) structures of your protein into your report. Note: structures have not been determined for all proteins, so you may have been given an identifier for a similar protein. Describe things about the structure, for example features of the shape, charge, or hydrophobicity.
Genome database assignment and stepwise instructions
Goal: improve student understanding of relationship between genes, protein(gene products), and biological functions.
The exercise:
Each student will be assigned a gene product/protein that will be di ...
Complete all three parts and answer all questions.Download Graph.docx
Complete all three parts and answer all questions.
Download
Graphic Organizer
for answering all questions
and genetics exercises in this assignment.
Part I: Genetics – From Genes to Proteins, Mutations
Background:
DNA represents the architectural blueprint for all living systems, encoding specific instructions in the sequence of its four nitrogen-containing base pairs that are necessary for building the organism.
During the process of transcription, the information in the DNA codons of a gene is transcribed into RNA.
A change in the DNA sequence, for example as a result of a "mistake" during DNA replication, is defined as a mutation. Mutations may result in a change in the "blueprint," which may then change the resulting protein product.
Assignment details for part one:
Transcribe and translate EACH of the
three
following DNA gene sequences.
Turn these in using the graphic organizer. The letters represent the DNA N-base sequences of the genes.
The first is the original gene. The next two are mutations of the original. Note that the changes (mutations) are shown in red.
Original Gene sequence 3'-T A C C C T T T A G T A G C C A C T-5
Mutated gene sequence 1 3’-T A C
G
C T T T A G T A G C C A
T
T-5'
Mutated gene sequence 2 3’-T A
A
C C T T T A
C
T A G
G
C A C T-5’
Also, answer the following questions:
What is the significance of the first and last codons of an mRNA transcript?
What meaning do these mRNA codons have for protein synthesis?
Did the mutations result in a change in the final proteins? If so, describe the change.
In general, why might a change in amino acid sequence affect protein function?
Part II: Inheritance of Traits or Genetic Disorders
Background
: Cystic fibrosis is an inherited disease that affects the respiratory, digestive, or reproductive systems of the body, with symptoms ranging from mild to severe. Bob and Sally are recently married. Upon deciding to plan a family, both Sally and Bob find out that they are both
heterozygous
for
cystic fibrosis
, but neither of them has symptoms of the disorder.
Assignment details:
Complete a
Punnett Square for cystic fibrosis
for this couple using the following alleles:
C = normal allele
; and
c = allele for cystic fibrosis
.
Complete the Punnett Square in the graphic organizer.
Also answer the following questions:
Based on the Punnett square, calculate chances (percentages) for the following:
for having a healthy child (not a carrier)
a child that is a carrier for the cystic fibrosis trait
a child with cystic fibrosis
Be sure to submit these percentages as part of your assignment in the Graphic Organizer.
Part III: Cell division, sexual reproduction and genetic variability
Background
: Eukaryotic cells can divide by mitosis or meiosis. In humans, mitosis produces new cells for growth and repair; meiosis produces sex cells (gametes) called
sperm
and
eggs.
Although mutations are the ultimate source of genetic variability, both meiosis and sexual reprod.
Nucleic Acids Structure and Functions
Introductory lecture to structure and functions of nucleic acids
Genes code for gene products. What does this statement mean Briefly.pdf
Genes code for gene products. What does this statement mean? Briefly describe the structure of
DNA by using the following terms: nucleotide, strand, complementary, deoxyribose, phosphate,
anti-parallel, base pairing, adenine, cytosine. How many DNA molecules are in a chromosome?
How many genes are in an average bacterial chromosome? What is the purpose of DNA
replication? (\"To make more DNA\" would not be a complete answer.) Summarize the process
of DNA replication by using the following terms: replication fork, template, nucleotide, primer,
DNA polymerase, DNA ligase. (Know what each term means.) Summarize the process of RNA
synthesis (transcription) by using the following terms: template, promo RNA polymerase,
rRNA, mRNA, tRNA, terminator. (Know what each term means.) Where are operons found and
what is the advantage of organization of genes within operons? What are some fundamental
differences in organization of genes between prokaryotes and eukaryotes? How do eukaryotes
produce mRNA that they can use for translation? Summarize the process of protein synthesis
(translation) by using the following terms: genetic code, ribosome, mRNA, protein, amino acid,
tRNA, codon, anticodon, start codon, stop codons, polypeptide. (Know what each term means.)
Be able to predict the sequence of a complementary strand in both DNA and RNA synthesis, w
hen the template sequence is given. Be able to use genetic code table to predict amino-acid
sequence of a encoded by a nucleic acid, when the nucleic acid sequence is given. Classify
mutations by type and briefly describe how mutations arise, are prevented or repaired. Explain
why mutations are important by giving at least three different examples. Why do bacteria and
viruses mutate so much faster than eukaryotes? What are some consequences of Briefly explain
(and be able to compare and contrast) three different mechanisms of horizontal gene in bacteria:
transformation, conjugation and transduction. What are some practical implications of these
phenomena?
Solution
3. Genes code for gene products
Gene is a portion of DNA. It is made up of nucleotide sequences. It expresses itself and transfer
from one generation to next generation.
Gene expression means the nucleotide sequence is used for synthesizing a biomolecules-
generally it is protein but it may be RNA molecules. Here gene products are those proteins and
RNAs.
4. Structure of DNA
DNA or deoxyribonucleic acid is the molecule that contains all genetic information of an
organism.
DNA has a double helix shape, which is like a ladder twisted into a spiral. Each spiral is
composed of polynucleotides. Each nucleotide is made up of - deoxyribose, a kind of sugar with
5 carbon atoms; a phosphate group made of phosphorus and oxygen, and nitrogenous base. There
are four types of nucleotide: Adenine (A), Thymine (T), Cytosine (C), Guanine (G). Nucleotides
are joined to one another by covalent bonds between the sugar of one nucleotide and the
phosphate of the next, kn.
Genes, Genomics, and Chromosomes computational biology introduction .ppt
The 5 ß-globin genes are derived from an ancestral ß-globin gene via gene duplication. Over time, these genes accumulated adaptive mutations via sequence drift resulting in the specialized species of ß-globin proteins. Genomic DNA also contains nonfunctional DNA sequences called pseudogenes that are derived from gene duplication or reverse transcription and integration of cDNA sequences made from mRNA (covered below). ß-globin pseudogenes contain introns and thus were derived by gene duplication. Over time these genes became nonfunctional also due to sequence drift. Because they are not harmful, pseudogenes remain in the genome, marking a gene duplication event in an earlier ancestor.
The ß-globin gene cluster on chromosome 11 is shown in Fig. 6.4a. The ß-globin genes are expressed in different stages of life. , Ag, and Gg are expressed during different trimesters of fetal development (next slide). ß expression begins around birth & continues throughout adult life. Fetal hemoglobin molecules made with the d and G or A polypeptides have a higher affinity for O2 than maternal hemoglobin, facilitating O2 transfer to the fetus.
Higher eukaryotes contain far more noncoding DNA between genes than bacteria and simple eukaryotes (Fig. 6.4). The region of human genomic DNA containing the ß-globin gene cluster shown in the figure actually is a relatively "gene-rich" region of human DNA. Some regions known as gene-poor "deserts" also occur. Higher eukaryotes also contain a larger amount of intron DNA. Although one-third of human DNA is transcribed into pre-mRNA, 95% ends up being degraded after RNA splicing reactions. On average, the typical exon is 50-200 bp in length, while the median length of introns is 3.3 kb in human genes.
DNA fingerprinting is a method for identifying individuals based on their minisatellite DNA (Fig. 6.7). It was developed in the mid-80s and is widely used in forensics, paternity analysis, and for research purposes. In the method, minisatellite DNA from a genomic DNA specimen is amplified by PCR using primers that bind to unique sequences flanking minisatellite repeat units. Bands corresponding to each minisatellite locus then are separated on gels. Although satellite DNA is highly conserved in sequence, the number of tandem copies at each loci is highly variable between individuals. This results from unequal crossing over during formation of gametes in meiosis. Due to the variation in the number of repeats at each locus, different individuals can be readily distinguished based on banding patterns.
Interspersed repeat DNA comprises the largest fraction of repetitious DNA in eukaryotic genomes. This DNA, which is also called moderately repeated DNA makes up ~45% of human genomic DNA. Interspersed repeat DNA is composed of partial and complete transposon sequences or "mobile DNA". Mobile DNAs were discovered by Barbara McClintock in the 1940s. These sequences move by "transposition". Transpositions in germ line cells are inhe
Please find the answers and explanations belowPart 1 (TrueFalse).pdf
Please find the answers and explanations below:
Part 1 (True/False)
1. Replication and transcription proceed in 5\' to 3\' direction: TRUE (Both DNA replication and
transcription of mRNA proceed in 5\' to 3\' direcition. This is because the firstly, the DNA
polymerase retains only 5\' to 3\' directionality and thus, replication begins only in this direction.
.Secondarily, there must be sequence similarity between the DNA and the RNA transcribed from
it thus the orientation of transcription is necessarily 5\' to 3\' in nature)
2. All cells have telomerase activity : FALSE (Telomerase or terminal transferase is an enzyme
which is required for maintenance of terminal positions of chromosomes by self-replication and
prevents shortening of chromosomes. It also prevents sticking of chromosomes together and thus
clumping of chromosomes. Telomerase acitivty is absent in majority of somatic cells in the
body.)
3. Only mRNA is transcribed: TRUE (Only the mRNA which carries the necessary information
for translation is transcribed from the DNA template. The tRNA is utilized to transfer the
transcribed information and rRNA is utilized to generate the polypeptide on the ribosomal
surface during translation)
4. Promoter regions are upstream from the gene: TRUE (A gene promoter is the region of DNA
located upstream the gene to be transcribed and carries the consensus sequence which could be
recognized by RNA polymerase for its binding and carrying on transcription)
5. A single tRNA can carry several different amino acids: FALSE (The tRNA is specific for only
one kind of amino acid which it could carry. It is a highly stringent and specific nature of this
biomolecule which determines the specificness for the amino acid which will be synthesized
from the codon)
Part 2
Please find the definitions below:
1. Semi-conservative replication: The mode of replication of double stranded DNA in which a
daughter DNA molecules carries a strand from parent DNA and the complementary strand is
synthesized de novo is called semi-conservative replication.
2. Okazaki fragments: Okazaki fragments are short stretches of DNA synthesized
discountinuously on the lagging strand on the open frame of the parent DNA. These stretches of
DNA are finally conjoined together to form a continous strand of newly synthesized DNA.
3. Consensus sequence: Consensus sequences are those genetically conserved sequences of DNA
which remain similar (upto 99%) within organisms of same or different species and are actively
engaged in crucial functions such as promoter region binding, transcription sites for transcription
factors, enzymatic genes etc. Any change or deterioration in these consesus sequence might be
highly deletrious for the cells.
4. Codon: A codon can be defined as a triplet set of nucleotides which come in a specific order
and encode for a specific type of amino acid upon translation. For example, the codon AAA
encodes for amino acid lysine. Exceptionally, codons UAA, UAG and UGA do not .
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Website – https://lembs.com/pmoschool
Youtube – https://www.youtube.com/startuplviv
FB – https://www.facebook.com/pmdayconference
Meas_Dylan_DMBS_PB1_2024-05XX_Revised.pdf
Personal Brand Statement:
As an Army veteran dedicated to lifelong learning, I bring a disciplined, strategic mindset to my pursuits. I am constantly expanding my knowledge to innovate and lead effectively. My journey is driven by a commitment to excellence, and to make a meaningful impact in the world.
Cracking the Workplace Discipline Code Main.pptx
Cultivating and maintaining discipline within teams is a critical differentiator for successful organisations.
Forward-thinking leaders and business managers understand the impact that discipline has on organisational success. A disciplined workforce operates with clarity, focus, and a shared understanding of expectations, ultimately driving better results, optimising productivity, and facilitating seamless collaboration.
Although discipline is not a one-size-fits-all approach, it can help create a work environment that encourages personal growth and accountability rather than solely relying on punitive measures.
In this deck, you will learn the significance of workplace discipline for organisational success. You’ll also learn
• Four (4) workplace discipline methods you should consider
• The best and most practical approach to implementing workplace discipline.
• Three (3) key tips to maintain a disciplined workplace.
Sustainability: Balancing the Environment, Equity & Economy
[Note: This is a partial preview. To download this presentation, visit:
https://www.oeconsulting.com.sg/training-presentations]
Sustainability has become an increasingly critical topic as the world recognizes the need to protect our planet and its resources for future generations. Sustainability means meeting our current needs without compromising the ability of future generations to meet theirs. It involves long-term planning and consideration of the consequences of our actions. The goal is to create strategies that ensure the long-term viability of People, Planet, and Profit.
Leading companies such as Nike, Toyota, and Siemens are prioritizing sustainable innovation in their business models, setting an example for others to follow. In this Sustainability training presentation, you will learn key concepts, principles, and practices of sustainability applicable across industries. This training aims to create awareness and educate employees, senior executives, consultants, and other key stakeholders, including investors, policymakers, and supply chain partners, on the importance and implementation of sustainability.
LEARNING OBJECTIVES
1. Develop a comprehensive understanding of the fundamental principles and concepts that form the foundation of sustainability within corporate environments.
2. Explore the sustainability implementation model, focusing on effective measures and reporting strategies to track and communicate sustainability efforts.
3. Identify and define best practices and critical success factors essential for achieving sustainability goals within organizations.
CONTENTS
1. Introduction and Key Concepts of Sustainability
2. Principles and Practices of Sustainability
3. Measures and Reporting in Sustainability
4. Sustainability Implementation & Best Practices
To download the complete presentation, visit: https://www.oeconsulting.com.sg/training-presentations
Digital Transformation and IT Strategy Toolkit and Templates
This Digital Transformation and IT Strategy Toolkit was created by ex-McKinsey, Deloitte and BCG Management Consultants, after more than 5,000 hours of work. It is considered the world's best & most comprehensive Digital Transformation and IT Strategy Toolkit. It includes all the Frameworks, Best Practices & Templates required to successfully undertake the Digital Transformation of your organization and define a robust IT Strategy.
Editable Toolkit to help you reuse our content: 700 Powerpoint slides | 35 Excel sheets | 84 minutes of Video training
This PowerPoint presentation is only a small preview of our Toolkits. For more details, visit www.domontconsulting.com
Evgen Osmak: Methods of key project parameters estimation: from the shaman-in...
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Kyiv PMDay 2024 Summer
Website – www.pmday.org
Youtube – https://www.youtube.com/startuplviv
FB – https://www.facebook.com/pmdayconference
Kseniya Leshchenko: Shared development support service model as the way to ma...
Kseniya Leshchenko: Shared development support service model as the way to make small projects with small budgets profitable for the company (UA)
Kyiv PMDay 2024 Summer
Website – www.pmday.org
Youtube – https://www.youtube.com/startuplviv
FB – https://www.facebook.com/pmdayconference
Enterprise Excellence is Inclusive Excellence.pdf
Enterprise excellence and inclusive excellence are closely linked, and real-world challenges have shown that both are essential to the success of any organization. To achieve enterprise excellence, organizations must focus on improving their operations and processes while creating an inclusive environment that engages everyone. In this interactive session, the facilitator will highlight commonly established business practices and how they limit our ability to engage everyone every day. More importantly, though, participants will likely gain increased awareness of what we can do differently to maximize enterprise excellence through deliberate inclusion.
What is Enterprise Excellence?
Enterprise Excellence is a holistic approach that's aimed at achieving world-class performance across all aspects of the organization.
What might I learn?
A way to engage all in creating Inclusive Excellence. Lessons from the US military and their parallels to the story of Harry Potter. How belt systems and CI teams can destroy inclusive practices. How leadership language invites people to the party. There are three things leaders can do to engage everyone every day: maximizing psychological safety to create environments where folks learn, contribute, and challenge the status quo.
Who might benefit? Anyone and everyone leading folks from the shop floor to top floor.
Dr. William Harvey is a seasoned Operations Leader with extensive experience in chemical processing, manufacturing, and operations management. At Michelman, he currently oversees multiple sites, leading teams in strategic planning and coaching/practicing continuous improvement. William is set to start his eighth year of teaching at the University of Cincinnati where he teaches marketing, finance, and management. William holds various certifications in change management, quality, leadership, operational excellence, team building, and DiSC, among others.
20240425_ TJ Communications Credentials_compressed.pdf
"𝑩𝑬𝑮𝑼𝑵 𝑾𝑰𝑻𝑯 𝑻𝑱 𝑰𝑺 𝑯𝑨𝑳𝑭 𝑫𝑶𝑵𝑬"
𝐓𝐉 𝐂𝐨𝐦𝐬 (𝐓𝐉 𝐂𝐨𝐦𝐦𝐮𝐧𝐢𝐜𝐚𝐭𝐢𝐨𝐧𝐬) is a professional event agency that includes experts in the event-organizing market in Vietnam, Korea, and ASEAN countries. We provide unlimited types of events from Music concerts, Fan meetings, and Culture festivals to Corporate events, Internal company events, Golf tournaments, MICE events, and Exhibitions.
𝐓𝐉 𝐂𝐨𝐦𝐬 provides unlimited package services including such as Event organizing, Event planning, Event production, Manpower, PR marketing, Design 2D/3D, VIP protocols, Interpreter agency, etc.
Sports events - Golf competitions/billiards competitions/company sports events: dynamic and challenging
⭐ 𝐅𝐞𝐚𝐭𝐮𝐫𝐞𝐝 𝐩𝐫𝐨𝐣𝐞𝐜𝐭𝐬:
➢ 2024 BAEKHYUN [Lonsdaleite] IN HO CHI MINH
➢ SUPER JUNIOR-L.S.S. THE SHOW : Th3ee Guys in HO CHI MINH
➢FreenBecky 1st Fan Meeting in Vietnam
➢CHILDREN ART EXHIBITION 2024: BEYOND BARRIERS
➢ WOW K-Music Festival 2023
➢ Winner [CROSS] Tour in HCM
➢ Super Show 9 in HCM with Super Junior
➢ HCMC - Gyeongsangbuk-do Culture and Tourism Festival
➢ Korean Vietnam Partnership - Fair with LG
➢ Korean President visits Samsung Electronics R&D Center
➢ Vietnam Food Expo with Lotte Wellfood
"𝐄𝐯𝐞𝐫𝐲 𝐞𝐯𝐞𝐧𝐭 𝐢𝐬 𝐚 𝐬𝐭𝐨𝐫𝐲, 𝐚 𝐬𝐩𝐞𝐜𝐢𝐚𝐥 𝐣𝐨𝐮𝐫𝐧𝐞𝐲. 𝐖𝐞 𝐚𝐥𝐰𝐚𝐲𝐬 𝐛𝐞𝐥𝐢𝐞𝐯𝐞 𝐭𝐡𝐚𝐭 𝐬𝐡𝐨𝐫𝐭𝐥𝐲 𝐲𝐨𝐮 𝐰𝐢𝐥𝐥 𝐛𝐞 𝐚 𝐩𝐚𝐫𝐭 𝐨𝐟 𝐨𝐮𝐫 𝐬𝐭𝐨𝐫𝐢𝐞𝐬."
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BI 101 Homework 5
- 1. Buy here: http://student.land/bi-101-homework-5/ 1. Explain the four roles that DNA plays in cells? How are these roles influenced by DNA’s structure? Be sure you demonstrate your understanding of DNA’s structure in your answer. 2. Match the terms with the most suitable description. 3. Briefly explain the differences among messenger RNA, transfer RNA and ribosomal RNA in terms of the roles they play in transcription and translation and where they are found in the cell. 4. Using the genetic code table (Fig 10.11 on p 180), take the following DNA sequence and complete the following 5. Part of the coding sequence of a gene produces an mRNA sequence of 6. Review pp 178-185 in your book and view the following animation. Then complete the following table. You may need to watch it the video more than once to catch the details.