Trichoderma is a biocontrol agent that can be used to control plant pathogens and diseases. It acts through mycoparasitism, antibiosis, competition, and induced resistance. Trichoderma species like T. harzianum and T. viride are effective biocontrol agents that can control diseases caused by fungi like Fusarium, Pythium, and Rhizoctonia. Trichoderma is mass produced using liquid or solid state fermentation and formulated products are available from various companies for seed treatment, soil application, and other uses to promote plant growth and manage diseases in agricultural crops.
Biological control is the suppression of one organism by another. There are two modes of mechanisms namely direct and indirect. Here I focused on the direct mechanisms such as parasitism, predatism, antibiotic-mediated suppression, lytic enzymes and unregulated-waste products. with the help of these various direct mechanisms, the bio-control agents will compete the pathogen's activity.
Biological control is the suppression of one organism by another. There are two modes of mechanisms namely direct and indirect. Here I focused on the direct mechanisms such as parasitism, predatism, antibiotic-mediated suppression, lytic enzymes and unregulated-waste products. with the help of these various direct mechanisms, the bio-control agents will compete the pathogen's activity.
It is a biofertilizer that contains symbiotic Rhizobium bacteria which is the most important nitrogen-fixing organism. These organisms have the ability to drive atmospheric Nitrogen and provide it to plants. It is recommended for crops such as Groundnut, Soybean, Red-gram, Green-gram, Black-gram, Lentil, Cowpea, Bengal-gram and Fodder legumes, etc.
INTRODUCTION
Trichoderma -A Bio-Control Agent
General characteristics, PREPARATION OF MOTHER CULTURE, Materials required, Method of application, Precautions.
Mushroom cultivation
Agaricus bisporus
Straw mushroom cultivation
Temperate mushroom
How to cultivate button mushroom
White button mushroom
Methodology of mushroom cultivation
Study of button mushroom cultivation
Research in Mauritius
Types of mushroom:In particularly edible mushroom available in India and also i include other types of edible mushroom current status.I denoted mushroom structure,character,nutrient value and favour for their growth , in which contry and their favour also included.
Mushroom are good nutritional food sorces,Mushroom used as medical purposses,commercial use.Mushroom are used to produces varity of food recipes like soap,cutlet,omelet etc...
Its is very cheep and esily digestable one.It does not contain fat,so all person able to eat and make a good healty..
It is rich in proteins, minerals,vitamins, carbohydrates, riboflavin etc...it is healty and give all essential energy sources into our body.
Ecto and endomycorrhizae and their significanceRitaSomPaul
A part of Botany (Hons) syllabus in Mycopathology illustrates the basic differnces in ectomycorrhizae and endomycorrhizae as well as their significance
Trichoderma is a filamentous fungus that is widely distributed in the soil, plant material, decaying vegetation, and wood. It belongs to the family Hypocreaceae. They have high potential for colonizing their habitats and have various applications in food industry, agriculture, as a biocontrol agent with mechanism involving antibiosis, competition, mycoparasitism, promotion of plant growth, solubilization and sequestration of inorganic plant nutrients, inducing resistance and inactivating pathogen’s enzymes and also as a source of transgene. The major driving force for investigation of biocontrol with Trichoderma is sustainability. As a plant symbiont and effective mycoparasites, numerous species of this genus have the potential to become biofungicides. the extensive studies on Trichoderma, including its diverse physiological traits available, is still progressing and making these fungi versatile model organisms for research on both industrial fermentations as well as natural phenomena. Jasmine Chughasrani | Abhishikta Dasgupta | Rutuja Das "Applications of Trichoderma- A Review" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-5 | Issue-2 , February 2021, URL: https://www.ijtsrd.com/papers/ijtsrd38341.pdf Paper Url: https://www.ijtsrd.com/biological-science/botany/38341/applications-of-trichoderma-a-review/jasmine-chughasrani
It is a biofertilizer that contains symbiotic Rhizobium bacteria which is the most important nitrogen-fixing organism. These organisms have the ability to drive atmospheric Nitrogen and provide it to plants. It is recommended for crops such as Groundnut, Soybean, Red-gram, Green-gram, Black-gram, Lentil, Cowpea, Bengal-gram and Fodder legumes, etc.
INTRODUCTION
Trichoderma -A Bio-Control Agent
General characteristics, PREPARATION OF MOTHER CULTURE, Materials required, Method of application, Precautions.
Mushroom cultivation
Agaricus bisporus
Straw mushroom cultivation
Temperate mushroom
How to cultivate button mushroom
White button mushroom
Methodology of mushroom cultivation
Study of button mushroom cultivation
Research in Mauritius
Types of mushroom:In particularly edible mushroom available in India and also i include other types of edible mushroom current status.I denoted mushroom structure,character,nutrient value and favour for their growth , in which contry and their favour also included.
Mushroom are good nutritional food sorces,Mushroom used as medical purposses,commercial use.Mushroom are used to produces varity of food recipes like soap,cutlet,omelet etc...
Its is very cheep and esily digestable one.It does not contain fat,so all person able to eat and make a good healty..
It is rich in proteins, minerals,vitamins, carbohydrates, riboflavin etc...it is healty and give all essential energy sources into our body.
Ecto and endomycorrhizae and their significanceRitaSomPaul
A part of Botany (Hons) syllabus in Mycopathology illustrates the basic differnces in ectomycorrhizae and endomycorrhizae as well as their significance
Trichoderma is a filamentous fungus that is widely distributed in the soil, plant material, decaying vegetation, and wood. It belongs to the family Hypocreaceae. They have high potential for colonizing their habitats and have various applications in food industry, agriculture, as a biocontrol agent with mechanism involving antibiosis, competition, mycoparasitism, promotion of plant growth, solubilization and sequestration of inorganic plant nutrients, inducing resistance and inactivating pathogen’s enzymes and also as a source of transgene. The major driving force for investigation of biocontrol with Trichoderma is sustainability. As a plant symbiont and effective mycoparasites, numerous species of this genus have the potential to become biofungicides. the extensive studies on Trichoderma, including its diverse physiological traits available, is still progressing and making these fungi versatile model organisms for research on both industrial fermentations as well as natural phenomena. Jasmine Chughasrani | Abhishikta Dasgupta | Rutuja Das "Applications of Trichoderma- A Review" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-5 | Issue-2 , February 2021, URL: https://www.ijtsrd.com/papers/ijtsrd38341.pdf Paper Url: https://www.ijtsrd.com/biological-science/botany/38341/applications-of-trichoderma-a-review/jasmine-chughasrani
Trichoderma: A bio-control agent for management soil born diseasesSaurabh Kedar
The present study was undertaken to isolate, identify, and quantify Trichoderma species from different habitats of Rajshahi metropolitan area and the premises of the compost plant of the Nature Development Society (NDS].
Recent Advances in Biopesticides BY Ghulam Murtazamurtaza8513
Biopestides are being manufactured all across the world but due to limited resources the research in biopesticides is not upto the mark. however advancement has been made in recent decades to protect crops from the attack of different insect pest in order to meet the agricultural productivity.
An entomopathogenic fungus can act as a parasite of insects and kills or seriously disables them.Targets are distributed among 10 insect orders:
Hemiptera (59.6%), Coleoptera (40.9%), Lepidoptera (17.5%), Thysanoptera (14.6%), Orthoptera (9.4%), Diptera (7.0%), Hymenoptera (2.9%), Isoptera (2.3%), Siphonoptera (1.2%), and Blattodea(0.6%).
Direct Action Against The Pathogen
Genetic Modification Of The Host To Resist
Modification Of The Environment To Make It Unfavorable For Diseases Development.
Slide 1: Title Slide
Extrachromosomal Inheritance
Slide 2: Introduction to Extrachromosomal Inheritance
Definition: Extrachromosomal inheritance refers to the transmission of genetic material that is not found within the nucleus.
Key Components: Involves genes located in mitochondria, chloroplasts, and plasmids.
Slide 3: Mitochondrial Inheritance
Mitochondria: Organelles responsible for energy production.
Mitochondrial DNA (mtDNA): Circular DNA molecule found in mitochondria.
Inheritance Pattern: Maternally inherited, meaning it is passed from mothers to all their offspring.
Diseases: Examples include Leber’s hereditary optic neuropathy (LHON) and mitochondrial myopathy.
Slide 4: Chloroplast Inheritance
Chloroplasts: Organelles responsible for photosynthesis in plants.
Chloroplast DNA (cpDNA): Circular DNA molecule found in chloroplasts.
Inheritance Pattern: Often maternally inherited in most plants, but can vary in some species.
Examples: Variegation in plants, where leaf color patterns are determined by chloroplast DNA.
Slide 5: Plasmid Inheritance
Plasmids: Small, circular DNA molecules found in bacteria and some eukaryotes.
Features: Can carry antibiotic resistance genes and can be transferred between cells through processes like conjugation.
Significance: Important in biotechnology for gene cloning and genetic engineering.
Slide 6: Mechanisms of Extrachromosomal Inheritance
Non-Mendelian Patterns: Do not follow Mendel’s laws of inheritance.
Cytoplasmic Segregation: During cell division, organelles like mitochondria and chloroplasts are randomly distributed to daughter cells.
Heteroplasmy: Presence of more than one type of organellar genome within a cell, leading to variation in expression.
Slide 7: Examples of Extrachromosomal Inheritance
Four O’clock Plant (Mirabilis jalapa): Shows variegated leaves due to different cpDNA in leaf cells.
Petite Mutants in Yeast: Result from mutations in mitochondrial DNA affecting respiration.
Slide 8: Importance of Extrachromosomal Inheritance
Evolution: Provides insight into the evolution of eukaryotic cells.
Medicine: Understanding mitochondrial inheritance helps in diagnosing and treating mitochondrial diseases.
Agriculture: Chloroplast inheritance can be used in plant breeding and genetic modification.
Slide 9: Recent Research and Advances
Gene Editing: Techniques like CRISPR-Cas9 are being used to edit mitochondrial and chloroplast DNA.
Therapies: Development of mitochondrial replacement therapy (MRT) for preventing mitochondrial diseases.
Slide 10: Conclusion
Summary: Extrachromosomal inheritance involves the transmission of genetic material outside the nucleus and plays a crucial role in genetics, medicine, and biotechnology.
Future Directions: Continued research and technological advancements hold promise for new treatments and applications.
Slide 11: Questions and Discussion
Invite Audience: Open the floor for any questions or further discussion on the topic.
(May 29th, 2024) Advancements in Intravital Microscopy- Insights for Preclini...Scintica Instrumentation
Intravital microscopy (IVM) is a powerful tool utilized to study cellular behavior over time and space in vivo. Much of our understanding of cell biology has been accomplished using various in vitro and ex vivo methods; however, these studies do not necessarily reflect the natural dynamics of biological processes. Unlike traditional cell culture or fixed tissue imaging, IVM allows for the ultra-fast high-resolution imaging of cellular processes over time and space and were studied in its natural environment. Real-time visualization of biological processes in the context of an intact organism helps maintain physiological relevance and provide insights into the progression of disease, response to treatments or developmental processes.
In this webinar we give an overview of advanced applications of the IVM system in preclinical research. IVIM technology is a provider of all-in-one intravital microscopy systems and solutions optimized for in vivo imaging of live animal models at sub-micron resolution. The system’s unique features and user-friendly software enables researchers to probe fast dynamic biological processes such as immune cell tracking, cell-cell interaction as well as vascularization and tumor metastasis with exceptional detail. This webinar will also give an overview of IVM being utilized in drug development, offering a view into the intricate interaction between drugs/nanoparticles and tissues in vivo and allows for the evaluation of therapeutic intervention in a variety of tissues and organs. This interdisciplinary collaboration continues to drive the advancements of novel therapeutic strategies.
Professional air quality monitoring systems provide immediate, on-site data for analysis, compliance, and decision-making.
Monitor common gases, weather parameters, particulates.
Nutraceutical market, scope and growth: Herbal drug technologyLokesh Patil
As consumer awareness of health and wellness rises, the nutraceutical market—which includes goods like functional meals, drinks, and dietary supplements that provide health advantages beyond basic nutrition—is growing significantly. As healthcare expenses rise, the population ages, and people want natural and preventative health solutions more and more, this industry is increasing quickly. Further driving market expansion are product formulation innovations and the use of cutting-edge technology for customized nutrition. With its worldwide reach, the nutraceutical industry is expected to keep growing and provide significant chances for research and investment in a number of categories, including vitamins, minerals, probiotics, and herbal supplements.
Earliest Galaxies in the JADES Origins Field: Luminosity Function and Cosmic ...Sérgio Sacani
We characterize the earliest galaxy population in the JADES Origins Field (JOF), the deepest
imaging field observed with JWST. We make use of the ancillary Hubble optical images (5 filters
spanning 0.4−0.9µm) and novel JWST images with 14 filters spanning 0.8−5µm, including 7 mediumband filters, and reaching total exposure times of up to 46 hours per filter. We combine all our data
at > 2.3µm to construct an ultradeep image, reaching as deep as ≈ 31.4 AB mag in the stack and
30.3-31.0 AB mag (5σ, r = 0.1” circular aperture) in individual filters. We measure photometric
redshifts and use robust selection criteria to identify a sample of eight galaxy candidates at redshifts
z = 11.5 − 15. These objects show compact half-light radii of R1/2 ∼ 50 − 200pc, stellar masses of
M⋆ ∼ 107−108M⊙, and star-formation rates of SFR ∼ 0.1−1 M⊙ yr−1
. Our search finds no candidates
at 15 < z < 20, placing upper limits at these redshifts. We develop a forward modeling approach to
infer the properties of the evolving luminosity function without binning in redshift or luminosity that
marginalizes over the photometric redshift uncertainty of our candidate galaxies and incorporates the
impact of non-detections. We find a z = 12 luminosity function in good agreement with prior results,
and that the luminosity function normalization and UV luminosity density decline by a factor of ∼ 2.5
from z = 12 to z = 14. We discuss the possible implications of our results in the context of theoretical
models for evolution of the dark matter halo mass function.
The increased availability of biomedical data, particularly in the public domain, offers the opportunity to better understand human health and to develop effective therapeutics for a wide range of unmet medical needs. However, data scientists remain stymied by the fact that data remain hard to find and to productively reuse because data and their metadata i) are wholly inaccessible, ii) are in non-standard or incompatible representations, iii) do not conform to community standards, and iv) have unclear or highly restricted terms and conditions that preclude legitimate reuse. These limitations require a rethink on data can be made machine and AI-ready - the key motivation behind the FAIR Guiding Principles. Concurrently, while recent efforts have explored the use of deep learning to fuse disparate data into predictive models for a wide range of biomedical applications, these models often fail even when the correct answer is already known, and fail to explain individual predictions in terms that data scientists can appreciate. These limitations suggest that new methods to produce practical artificial intelligence are still needed.
In this talk, I will discuss our work in (1) building an integrative knowledge infrastructure to prepare FAIR and "AI-ready" data and services along with (2) neurosymbolic AI methods to improve the quality of predictions and to generate plausible explanations. Attention is given to standards, platforms, and methods to wrangle knowledge into simple, but effective semantic and latent representations, and to make these available into standards-compliant and discoverable interfaces that can be used in model building, validation, and explanation. Our work, and those of others in the field, creates a baseline for building trustworthy and easy to deploy AI models in biomedicine.
Bio
Dr. Michel Dumontier is the Distinguished Professor of Data Science at Maastricht University, founder and executive director of the Institute of Data Science, and co-founder of the FAIR (Findable, Accessible, Interoperable and Reusable) data principles. His research explores socio-technological approaches for responsible discovery science, which includes collaborative multi-modal knowledge graphs, privacy-preserving distributed data mining, and AI methods for drug discovery and personalized medicine. His work is supported through the Dutch National Research Agenda, the Netherlands Organisation for Scientific Research, Horizon Europe, the European Open Science Cloud, the US National Institutes of Health, and a Marie-Curie Innovative Training Network. He is the editor-in-chief for the journal Data Science and is internationally recognized for his contributions in bioinformatics, biomedical informatics, and semantic technologies including ontologies and linked data.
A brief information about the SCOP protein database used in bioinformatics.
The Structural Classification of Proteins (SCOP) database is a comprehensive and authoritative resource for the structural and evolutionary relationships of proteins. It provides a detailed and curated classification of protein structures, grouping them into families, superfamilies, and folds based on their structural and sequence similarities.
3. What is biological control, what are the benefits
to its use
Need of biological control agents
Mechanism of biological control agents
Requirements of successful biocontrol agents
Working example of biocontrol
agentTrichoderma ssp.
Available Biopesticides and its use in
Agriculture
4. Importance of plant diseases
Estimated annual crop production worldwide
Amount lost to disease, insects, weeds using
current control measures
Additional losses without current control measures
$1.2 - 1.3 trillion$1.2 - 1.3 trillion
$500 billion$500 billion
$330 billion$330 billion
Verticillium wiltVerticillium wilt
Citrus cankerCitrus canker
Fireblight of pearFireblight of pear Apple scab
Rice blast
( IMF and CIA World Factbook, 2014)
5. 2013: world spent $43.4 billion$43.4 billion on
chemical pesticides
Of this, < 1%< 1% actually gets to
where the pathogen is
What happens to the rest?
Ground water
Taken up by the plant
Development of resistance
Current approaches to disease control
Chemical
Identification of resistance genes
Introgressing into commercial
cultivars
Problems with development of
resistance, pyramiding genes
Breeding
Biological Control is an attractive alternative/supplement
6. Control of plant pathogens and diseases caused by them through antagonistic
microorganisms or botanicals is termed biological control agents
According to Baker and Cook’s (1974) - “Biological control is the
reduction of inoculum or disease producing activity of a pathogen
accomplished by or through one or more organisms other than man.”
Antagonistic microorganisms like species of Trichoderma, Penicillium,
Bacillus, Pseudomonas etc.
7. Chemical pesticides
Implicated in environmental and human health
problems
– Require yearly treatments and expensive
– Toxic to both beneficial and pathogenic species
• Biological control agents
– Non-toxic to human and animal
– Not polluted
– Host specific
•Only effect one or few species
8. WHEN :
Biological control agents are
◦ Low cost
◦ Labor intensive
◦ Host specific
WHILE :
Chemical pesticides are:
◦ cost-effective
◦ easy to apply
◦ Broad spectrum
9. How does Biological Control works
Nutrient Competition
Biological
Control
Antibiosismycoparasitism
Induced resistance
Tolerance to stress
through enhanced
root and plant
development
Inactivation of the
pathogen’s enzymes
10. An ideal biocontrol agent should satisfy
most all, of the following attributes
• Must not be pathogenic to plants and animals
• Level of pathogen control must be high
• Should live longer in soil or host tissues
• Should have rapid reproductive capacity
• Should be a good competitor
• Should have high survival rate in soil or host tissues
• Should be capable of controlling more than one
pathogen
• Should be suitable for long-term storage
• Should be compatible to use with agro-chemicals
viz. fertilizers, pesticides etc.
11.
12. 1671 – First found in Germany
1794 – Identified by Persoon almost 218 years ago
1927 – Gilman and Abbott recognized four species based on
colour, shape of conidia and colony appearance
>75 years ago the potential use of Trichoderma by Weindling
(1932) and first to demonstrate the parasitic activity in wilt of
Pigeon pea
Best known mycoparasite against many soil borne plant pathogens
13. Very effective biological agent
Free living
Highly proliferating
Non- pollutive
Easily accessible
Non phytotoxic
Systemic ephemeral
Readily biodegradable
Cost effective
Synergistic effect
Longer shelf life
Greater compatibility
14. A genus of fungi, including many species that can be used
to control phytopathogenic fungi.
generally, soil dwelling saprophytes. They have a rapid
growth rate, sporulate abundantly, compete well with other
show resistance to chemical pesticides and produce various
antibiotics (e.g., gliotoxin and viridin).
control of wood-rotting, wound-infecting and soil-borne
fungal pathogens of seedlings and mature plants.
Conidiophores
Conidia
Hyphae
Morphological structure of Trichoderma
15. Kulkarni and Sagar (2007) mentioned the Trichoderma as asexual stage
and Hypocrea as sexual stage
Position Asexual stage
(conidia)
Sexual stage
(ascospore)
Kingdom Fungi Fungi
Phylum Ascomycota Ascomycota
Sub-division Deuteromycotina Ascomycotina
Class Hyphomycetes Pyrenomycetes
Order Monilliales Sphariales
Family Monilliaceae Hypocreaceae
Genus Trichoderma Hypocrea
16. Cultures are fast growing at 25-30° C
Conidia forming within on week in compact
or loose tufts in shades of green or yellow
or less frequently white
Yellow pigment may be secreted into the
agar, specially on PDA
A characteristic sweet or ‘coconut’ odour
is produced by some species
19. Potential bio control activities exhibited by TrichodermaPotential bio control activities exhibited by Trichoderma
Kamala and Indira, 2012, Manipur
20. For space and nutrients under specific condition do not get substrate
Suppress growth of pathogen population
e.g: Soil treatment with Trichoderma harzianum spore suppressed
infestation of Fusarium oxysporum f. sp. vasinfectum and F. oxysporum f.
sp. melonis
(Perveen and Bokhari, 2012)
Mechanisms of action
21. Mycoparasitism
Antagonist fungi parasitize other pathogenic fungi
Hyphae of Trichoderma either grow along the host hyphae or coil
around it
E.g. : T. harzianum and T. hamatum were mycoparasite
of both Scelerotium rolfsii and R. solani
22. Interaction –
Coiling of hyphae around the pathogen,
Vacuolization,
Penetration by haustoria and
lysis (Omero et al., 1999).
Recognize and attach to the pathogenic fungus and excrete extra-cellular
lytic enzymes like β-1,3-glucanase, chitinase, proteases and lipase
(Schlick et al., 1994).
23. Trichoderma coils around, penetrates, and kills other fungi that are
pathogenic (i.e. cause disease) to crops. It can digest their cell walls
A clear view with an electron microscope
Trichoderma spp.(T) fungal strands
coil (C) around the Rhizoctonia (R)
Initial stages of degradation (D) as a result of
Trichoderma generated enzymes.
T: Trichoderma R: Rhizoctonia
24. Antibiosis
It is the condition in which one or more metabolites
excreted by an organism have harmful effect on one or more
other organisms
In such antagonistic relationship spp. A produces a chemical
substance that is harmful to Spp. B without a Spp. A deriving
any direct benefit
e.g: Trichoderma secreted - Trichodermin, viridine,
Trichothecin, Sesqiterpine etc.
25. Growth inhibition of R. solani by the T. virens produced antibiotic
gliotoxin . A: Gliotoxin amended B: non amended
Cont…
26. Trichoderma strains solubilize phosphates and micronutrients
The application of Trichoderma strains in rhizosphere of plants
increases the number of deep roots, there by increasing the plants
ability to resist drought
Plant growth promoter
27. Fig.: Enhanced root development from field grown bean plants as a consequence
of root colonization by the rhizosphere competent strain T. harzianum
(Amin et al., 2010)
Cont…
32. Organism Trade Name Target Crops
Trichoderma
virens
SoilGard 12G3 Pythium,
Rhizoctonia, and
Root rots
Ornamental and food crop plants grown in
greenhouses,
Trichoderma
harzianum Rifai
strain KRL-AG3
Plant Shield® HC Fusarium,
Pythium, and
Rhizoctonia
Cucurbit vegetables, flowers, bedding plants,
Trichoderma
harzianum Rifai
strain KRL-AG2
T-22™ HC Fusarium,
Pythium, and
Rhizoctonia
Agronomic field and row crops, alfalfa, hay and
forage crops, bulb crops, cucurbits, fruiting
vegetables, herbs, spices, leafy vegetables,
cole crops, legumes, root crops, small grains
and tuber crops
Trichoderma
harzianum Rifai
strain KRL-AG2
T-22™ Planter
Box
Fusarium,
Pythium, and
Rhizoctonia
Agronomic field and row crops, alfalfa, hay and
forage crops, bulb crops, cucurbits, fruiting
vegetables
33. There are several reputable companies that manufacture
government registered products.
Trade Name Bio agent Manufacture
Eco fit T. viride Hoechst and Schering AgroEvo Ltd,
Mumbai India
Super visit T. harzianum Fytovita, Czech Republic
Soil guard T. virens Certis Inc,Columbia,MD,USA
Root pro T. harzianum Efal Agri, Netanyl,Israel
Tusal T. Viride +T.
harzianum
Tusal Carrera Ester, Lleida Spain
Agroderma, Bio-cure,
Bioderma, Ecofit,
Rakshak, Trichosan
Trichoderma viride
Biocure (B&F) T. Viride and P. flourescens
34. 1.Seed treatment
•Dose: @5 g /kg of seed
•Method: Make a paste or slurry adding 5 g in 10-20 ml of water
. Pour 1kg of seed on to the paste or slurry and mixed properly
to coat the seeds uniformly .Shade dry the coated seeds for 20-
30 minutes before sowing
2.Tuber/Rhizome/Cutting treatment:
•Dose: @ 10 g /litre of water
•Method:Dip the tuber / rhizome/cuttings in the suspension
prepare @ 10 g /litre of water. Shade dry for 15 minutes before
planting
(Medhi,2009)
35. Seedling Treatment:
•Dose: 300 g/ha.
•Method: Prepare a suspension @ 5-10 g/litre of water. Dip the
roots of seedling for 15minutes and shade dry for 15 minutes
before transplanting
Nursery bed treatment:
•Dose: 250 g for 400 sq.m nursery bed.
•Method: 1) prepare a suspension by adding 250 g in 50 litres of
water and drench the nursery bed soil.
•2) Mixed 250 g in 2 kg cow dung / compost/FYM and spread
over 400 sq.m. nursery bed and irrigate the bed.
36. Soil treatment:
i) Direct broadcasting:
•Dose: 300 g/ha.
•Method: Mix 300 g in 6 kg of FYM. Broadcast in one ha of land and irrigate the
field.
ii) Awaited broadcasting
•Dose: 30 g/ha
•Method: Mixed 30 g in 6 kg of FYM. Cover the mixture with polythene sheet for 7-
15 days and broadcast in the field
iii) Furrow application:
•Dose:300g /ha.
•Method: It is highly effective in root crops like potato, ginger, turmeric etc and
sugarcane.The mixture is applied in furrows at the time of earthing up or after 30
days of planting
37. • Decrease disease intensity.
• Reduce the use of chemical fungicides.
•Reduce undesirable effects from chemical pesticide.
• Play a key role in integrated management of diseases
•Safe for the users and the farming community.
• Provide natural long term immunity to crops and
soil
( Shrivastava , 1996 ).
38. •Deterious effects on non-target micro-
organisms
•Pathogens may develop resistance to the
biocontrol agent
•Pathogen replacement may follow control of
target disease pathogen
•Seasonal/weather phenomena can make
biocontrol agent ineffective
( Shrivastava , 1996 ).
39. CONCLUSIONS
Biological control an attractive alternative to
chemicals
Trichoderma species are effective in biological control of
fungus-induced plant disease
Plant diseases cause major loss of food and
money
Biological control occurs via several mechanisms
Competition
Antagonism
Mycoparasitism