Rice bran , instead of using as animal feed, was stablized , studied from the phytochemical , pharmacological, toxicological and formulation points of view. It is developed to be used in bakery, nutraceutical, cosmoceutical preparations with soild scientific proof of evidence.

1. BY:
Dr. Amr Mohamed Helal
PhD Pharmacognosy and Medicinal Plants
EX- Lecturer , Pharmacognosy and Medicinal Plants ,
Faculty of Pharmacy Cairo University ( 1985 – 1997 )
KEAP For Biofertilisers , Managing Director
Rice-N- care ( Nutraceuticals) Managing Director
Rice Residues Management
Rice Bran, Straw & Husk
www.ricencare.com

2. Diamonds are buried and ignored in deserts and mountains
for thousands of years….
Till picked up , polished and then they
glow forever Egyptian Rice Residues could be these
diamonds … !!!!
(Remember ,, Diamonds are JUST
CARBON
)

3.  Paddy rice in Malaysia is roughly
 more than 2.5 Million Tons;
 giving more than 250 000MT
rice bran.
 However due to non stabilization of Rice Bran
it is still discarded or used as animal feed despite its
high content of bioactive substances
It is here where an UNTOLD WEALTH of vitamins,
minerals and newly discovered disease fighting
antioxidants are found as well as Nutraceuticals for
alleviation of major illness symptoms (CVD, DM type II,
elevated Cholesterol levels, Anti inflammatory &
Hypertension).

4. Rice Bran is considered as one of the most nutritive ingredients of the rice crop,
where about 60% of the total nutritive value of rice itself resides.
Rice BranPROTEINS
OIL DIETARY FIBREVITAMINS &
MINERALS
GAMMA
ORYZANOL
ANTIOXIDANTS
TRACE
ELEMENTS
RICE BRAN
These components can be obtained from other sources of nature separately, but Rice
Bran is unique in having all these constituents together.

5. Rice Bran Nutraceutical / Functional Food Values
Potent Antioxidant
High Nutritional
Value
Blood Pressure
Blood Cholesterol
POTENTIAL BENEFITS OF RICE BRAN
Blood Sugar

6. It All Starts with
RICE BRAN INNOVATIVE STABILIZATION

8. Investment in Science
A Highly Rewarded Investment
Non Stabilized Rice Bran; roughly estimated selling
price US$ 150
Stabilized Rice Bran;
As bakery ingredient; US$ 250/ MT (20 %
replacement of wheat flour with better taste and more
nutritional data). This can utilize the majority of Rice
Bran in Malaysia
As Functional Food , US$ US$ 500 / MT ( For
elderly people, School children, lactating women,
athletics..etc)

9. NON Stabilized Rice Bran Extract
Polutry Feed; US$ 1 / liter
Rice Bran Oil; US$ + 1.5 / Liter
Stabilized Rice Bran Extract
Nutraceutical Purposes; US$ 50/ liter
Clinically tried Products; US$ !!!!!
Nano Cosmoceutical preparations;
US$ !!!!!!
Anti aging preparations; US$ !!!!!
(Except the clinical trials, all above knowledge are available
at ITM for Egyptian Rice bran)

10. A) Rice Bran Stabilization (lab Scale) & (Industrial
Scale)
B) Phytochemical and Nutritional study of the Stabilized
Rice bran.
C) Implementations of the different rice bran products
in food , pharmaceutical and cosmetic industries .

12. Rice Bran – Stabilization Unit 2
Capable of stabilizing the bran of 150 MT rice milling / day

13. Rice Bran Nutraceuticals
 Contract Number C2/S1/118
 Total cost of the action EURO 192000
 Project Duration 18 months
 Project PI Prof. Dr. M.T. Khayyal
 Project Coordinator Dr. Amr M. Helal

14.  Faculty of Pharmacy; Cairo University FOPCU (Applicant)
 International trade and Marketing IT&M (Coordinator)
 Faculty of Pharmacy; German University in Cairo GUC
 Faculty of Pharmacy; Johann Wolfgang Goethe University
Frankfurt (GUF)
 Faculty of Pharmacy, Munster
 VivaCell Biotechnology GmbH VC (Germany)

15. •hypertensive activity-Anti
Rice bran extract has definite potential to lower blood pressure inResult:
animals L-NAME induced hypertension
•inflammatory activity-Anti
inflammatory effects against acute‐Rice bran extract exerts potent antiResult:
and chronic inflammation in vivo.
•Hypocholesterolaemic activity
Rice Bran extract is as effective in lowering cholesterol in vitro asResult:
pravastatin; a commercial available drug
•Antioxidant activity
Rice Bran extract has an effective antioxidant activityResult:
•diabetic activity-Anti
diabetic activity-Rice Bran extract has antiResult:
Pharmacological Activity Prof. Dr. M. T. Khayyal
Project PI

16. Acute Toxicity Studies
g/Kg2Rice Bran extract showed no acute toxicity >Result:
Chronic Toxicity Studies
Rice Bran extract showed no toxic effect on body weight,Result:
haematological parameters, liver & kidney functions, blood
glucose, electrolytes, etc
:AMES Test
: Rice Bran extract showed no mutagenic activity on theResult
DNA.
Toxicological Activity; Prof. Dr. Ola A. Heikal GUC

17.  Rats were rendered hypertensive by the intra-
peritoneal injection of L-NAME twice daily for 1 week.
By the end of that period, the animals showed a
significant rise in blood pressure.
 Hypertensive rats were treated orally for 1-2 weeks
with Rice Bran Extract (RBEX).
 Systolic blood pressure (SBP) and heart rate (HR) of
animals measured non-invasively.

18. 100
110
120
130
140
150
160
170
180
Normal L-NAME RBEX 30 mg/kg RBEX 100 mg/kg
SBP[mmHg]
Week1 Week2

19. 200
250
300
350
400
450
Normal L-NAME RBEX 30 mg/kg RBEX 100 mg/kg
HR[Beats/min]
Week0 Week1 Week2

20.  Rice bran extract has definite potential to
lower blood pressure in animals with
established hypertension.
 Antihypertensive effect is not associated with
appreciable changes in heart rate.

21.  Standardized rice bran extract (SRB) tested on 2
models of inflammation:
Acute model: Carrageenan rat paw oedema
Chronic model: Adjuvant – induced arthritis
 Samples of blood taken to determine:
a) myeloperoxidase: measure of antioxidant
activity
b) TNFα: mediator of inflammation.
c) IL – 10: anti-inflammatory mediator.

22. 0
0.2
0.4
0.6
0.8
1
1.2
1.4
Changeinpawvolume
Normal
Control
Diclofenac Na (5 mg/kg)
Rice Bran Extract (30 mg/kg)
Rice Bran Extract (100 mg/kg)
*
@ *
@ *
@ *

23.  Rats rendered arthritic by subplantar injection of
Freund‘s complete adjuvant in right hind paw.
 SRBE and diclofenac (reference standard drug)
given orally at same time and continued for 28 d.
 Paw volume measured at regular intervals.
 Blood samples collected for measuring mediators
(TNFα and IL-10) and myeloperoxidase (MPO)
activity.

24. 0
1
2
3
4
5
0 4 7 14 21 28
days
Changeinpawvolume(ml)
Control
Diclofenac (5mg/kg)
RBE (30 mg/kg)
RBE (100 mg/kg)
**
*
*
*
*
**
*

25. 0
0.1
0.2
0.3
0.4
0.5
0.6
0.7
0.8
0.9
1
MPO(U/ml)
Normal
Control
Diclofenac (5 mg/kg)
RBE (30 mg/kg)
RBE (100 mg/kg)

26. 0
5
10
15
20
25
30
35
40
45
50
TNF-α(pg/ml)
Normal
Control
Diclofenac (5 mg/kg)
RBE (30 mg/kg)
RBE (100 mg/kg)
*
@ @
@

27. 0
20
40
60
80
100
120
140
160
180
IL-10(pg/ml)
Normal
Control
Diclofenac (5 mg/kg)
RBE (30 mg/kg)
RBE (100 mg/kg)
*
*

29.  Rice bran extract exerts potent anti-inflammatory
effects against acute and chronic processes in
vivo.
 Effect associated with some anti-oxidant activity
and inhibition of inflammatory mediators (TNFα)
as well as enhancing antiinflammatory ones(IL-10)
 In vivo effects supported by in vitro findings

30.  Rice bran extract was incubated with neuro-
blastoma cells (SH-SY5Y) in vitro for 12 h.
 Unesterified cholesterol levels measured.
 Effect compared with pravastatin

32.  RBE was nearly as effective in lowering
unesterified cholesterol in vitro as pravastatin
which was taken as a representative of the
statin family known for its potent blood
cholesterol lowering effect.
 Results encouraging: in vivo experiments in
progress

33.  RBE incubated with mouse brain homogenates.
 After incubation, homogenates cenrifuged, and
supernatants tested for lipid peroxidation by
measuring malondialdehyde (MDA) and
hydroxynonenal (HNE) levels
 Effect compared with Trolox (Vit. E derivative)
known to have antioxidant activity.

35.  Acute and Subchronic Toxicity studies (28
days).
 Acute results showed no toxic effects > 2g/Kg
 Chronic testing 0.5g, 1g and 2g/kg/day orally
to male and female rats for 28 days.
Examination of effect on body weight,
haematological parameters, liver & kidney
functions, blood glucose, electrolytes, etc…
 Organs examined histologically at end of study.

36. F( control) F( 1g/kg) F(0.5g/kg)
SGPT 46.8 42 32.6
SGOT 141.5 186.2 118
0
50
100
150
200
250
U/ml
Female
**
M ( control) M( 1g/kg) M (0.5g/kg)
SGPT 51 47.7 36.8
SGOT 186.6 129.75 174.4
0
50
100
150
200
250
Male

37. F( control) F(1g/kg) F(0.5g/kg)
Alkaline phosphates 95.3 109 142
0
50
100
150
200
U/L
Female
M( control) M(1g/kg) M(0.5g/kg)
Alkaline phosphates 151 191 188.4
0
50
100
150
200
250
300
U/L
Male

38. F( control) F( 1g/kg) F(0.5g/kg)
Creatinine 0.78 1.03 0.82
0
0.5
1
1.5
mg%
Female
M(control) M(1g/kg) M(0.5g/kg)
Creatinine 0.88 0.84 0.96
0
0.5
1
1.5
mg%
Male

39. F(control) F(1g/kg) F(0.5g/kg)
Blood -glucose 53.5 37.5 54.6
0
10
20
30
40
50
60
70
mg%
Female
M(control) M(1g/kg) M(0.5g/kg)
F.Blood glucose 44.6 58.6 36.6
0
10
20
30
40
50
60
70
80
mg%
Male

40. F( control) F(1g/kg) F(0.5g/kg)
Sodium 187.7 163.2 163.2
0
50
100
150
200
250
mmol/L
Female
M( control) M(1g/kg) M(0.5g/kg)
Sodium 170 167.8 153.8
0
20
40
60
80
100
120
140
160
180
200
mmol/L
Male

41. F( control) F(1g/kg) F(0.5g/kg)
Potassium 7.6 8.1 7.2
0
2
4
6
8
10
12
mmol/L
Female
M( control) M(1g/kg) M(0.5g/kg)
Potassium 8.66 11.07 10.32
0
2
4
6
8
10
12
14
mmol/L
Male

42. Rice Bran Formulation
•Soft Gelatin Capsules (Extract) (500mg)
•Bran Tablets (1 gm)
•Bran Sachet Powder (10gm sachets)
Rice Bran tests for registration file
•Heavy Metal Analysis
•Pesticide Residue Analysis
•Microbial Analysis
•Aflatoxin Analysis
•Non Genetic Mutation Origin Analysis
Formulation & Production

43. Rice Bran Nutraceuticals
Project’s New Ventures
CosmoceuticalsRice Bran
•Preparation of Rice Bran Extract in the Nano form
•Preparation of cosmetic preparations (Emollient,
Nourishing & Rejuvenating creams and lotions)
Rice Bran anti aging effect !!
•Rice Bran extract showed significant protection for brain
nerve cells against oxidation
( a new discovery is submitted for publication)

44. Rice Bran Nutraceuticals
Project Outcomes
•Converting low value residues into high value lucrative products
•Development of a Nutracutical formulation that has a great beneficial
impact on the Health Sector of the country
Anti-Hypertension
Anti- Diabetic
Anti-inflammatory
Anti-oxidant
Hypocholesterolaemic
Anti-aging
•Development of a Novel ingredient that benefits and is easily integrated in
several industries
Food products + Rice Bran = Functional Food
Cosmetics + Rice Bran (Nano) = Cosmecuticals

45. www.ricencare.com

46. RDI Projects
Direct Impacts
Indirect Impacts
Local Marketing
International Marketing

50. Thanks for Listening and
Wish all the success for all of You
And Wish within soon
EGYPT and Malysia start to turn
Rice bi-products into GOLD MINES
Amr Helal