TALENs is a technique that enables targeted modification of DNA sequences in living cells and organisms. It has been used to provide plant resistance against bacterial wilt disease by editing the DNA binding code. TALENs has also been used to edit genes in mouse embryonic stem cells to produce mice with modifications to two Y-linked genes. Additionally, TALENs can construct fusion genes associated with certain cancers at their endogenous loci in human cells.

1. Transcription activator like effector nucleases is widely
applicable technique which enables modification of
sequence of interest in living cells and organisms.

2. Bacterial wilt disease is a major crop
disease caused by Ralstonia
solanacearum. This plant pathogen infect
many economically important crops such
as pepper ,tomato, potato tobacco etc.so
the TALENs is used to break the DNA
binding code to provide plant resistant
against bacterial wilt disease.

3. The use of mice is important to unravel the biology of Y
chromosome. TALEN’s is used to edit genes in mouse
embryonic stem cells to produce mice with two Y linked
genes Sry and Uty.

4. Chromosomal translocation causes various cancers due to
formation of fusion genes which act as oncogenes. TALENs
is use to construct the translocation associated oncogenes
in cognate endogenous loci in human cells.

5. TALENS is used to modify variety of genes in livestock
such as pig and cattle. Gene KO is used to improve
valuable models for human disease also important for
alteration of production trait and reducing treat of zoonotic
disease of livestock.

6. TALENs were designed to engineer the human loci CCR5
and EGFP marker gene which helps to cure HIV1 acute
myeloid leukamia .

7. Here TALENs is used to disrupt targeted genes tyrosine
(try)and pax6 that are involved in pigmentation and eye
formation of xenopus laevis embryos. Introduction of this
TALENs engineered genes in to embryos results drastic
loss of pigmentation and deformed eyes.