COMPARATIVE STUDY OF ANTI-ULCER POTENTIAL OF OMEPRAZOLE, RANITIDINE AND DOMPERIDONE IN ASPIRIN INDUCED ULCER RAT MODEL. OBJECTIVE : To compare anti-ulcer potential of Omeprazole, Ranitidine and Domperidone (Prokinetic agent) in Aspirin induced ulcer rat model.

1. COMPARATIVE STUDY OF ANTI-
ULCER POTENTIAL OF
OMEPRAZOLE, RANITIDINE AND
DOMPERIDONE IN ASPIRIN
INDUCED ULCER RAT MODEL
RANA SAHA
ROLL NO. 27701916062
B.PHARM. 7TH SEM

2. OBJECTIVE
TO COMPARE ANTI-ULCER POTENTIAL OF OMEPRAZOLE, RANITIDINE
AND DOMPERIDONE (PROKINETIC AGENT) IN ASPIRIN INDUCED
ULCER RAT MODEL.

3. RESEARCH QUESTIONS
1
•WHY HIGH DOSE OF ASPIRIN CAUSES GASTRIC ULCER ?
2
•HOW A H2 ANTAGONISTS (RANITIDINE) ACT ?
•HOW A PROTON PUMP INHIBITORS (OMEPRAZOLE) ACT ?
3
•WHOSE ANTIULCER POTENTIAL IS HIGHER IN BETWEEN
RANITIDINE AND OMEPRAZOLE ?
4
•WHAT IS THE EFFECT OF DOMPERIDONE ON ULCER ?

4. PEPTIC ULCER
Peptic ulcer disease (PUD) or
stomach ulcer, is a break in
the inner lining of the
stomach, first part of the
small intestine or sometimes
the lower esophagus. An
ulcer in the stomach is
called a gastric ulcer, while
that in the first part of the
intestines is a duodenal
ulcer.

5. ASPIRIN
INDUCED
ULCER
Acute hemorrhagic
gastritis occurs in from
50% to 70% of all
patients taking Aspirin
and other NSAID is not
directly related to dose
size, and can be severe
enough to cause death
in a few cases.

6. MATERIAL : ANIMAL
(CPCSEA Reg. No. 1458/PO/E/11/CPCSEA)
1) Species : rat
2) Strain : wistar
3) Source : NSHM college animal house
4) Sex : male
5) Age : 7-10 weeks
6) Body weight range : 200-250g
7) No. Of animals /group : 06
8) No. Of group : 05

7. INDUCTION OF ULCER
ULCER CAN BE INDUCED IN THE ANIMAL BODY BY TWO METHODS –
A) PYLORUS-LIGATION-INDUCED PEPTIC ULCER (SHAY’S METHOD)
B) ASPIRIN INDUCED ULCER

8. ASPIRIN INDUCED ULCER
All groups of rats were treated with test drugs for 7 days prior to aspirin induced
ulcer. Animals were divided into 5 groups and treated with drugs as in above
model. After 7 days of treatment, animals were fasted for 24 hrs. Ulcers were
produced by oral administration of aspirin (500mg/kg) on the day of sacrifice. The
animals were sacrificed 4 hours later and stomachs were open along the greater
curvature and ulcers were graded. Percentage of ulcer protection, ulcer index were
observed and calculated.

9. EXPERIMENT DESIGN
Sl.
No.
Group Test Group
Treated
Drug
Dosages
1. I
Administered vehicle serves
as Normal control
1% CMC 0.2 ml
1. II
Gastric ulcers induced by
Aspirin
Aspirin 500mg/kg
1. III
Aspirin induced ulcer rats
treated with Omeprazole
Omeprazole 20 mg/kg
1. IV
Aspirin induced ulcer rats
treated with Ranitidine
Ranitidine
300
mg/kg
1. V
Aspirin induced ulcer rats
treated with Prokinetic agent
Prokinetic
agent
40 mg/kg

10. PARAMETERS FOR THE EVALUATION OF THE
ANTIULCER POTENTIAL OF THE TEST DRUGS
1) Volume of gastric secretion
2) Total acidity (hawk, 1965)
3) Total acid output (goel,
1985)
4) Measurement of gastric
lesions
5) The ulcer index

11. THE ULCER INDEX
ULCER INDEX (UI)=(TOTAL ULCER SCORE)/(NO. OF ANIMALS
ULCERATED)
The ulcer index
0 = no lesion,
1 = mucosal oedema and petechiae,
2 = one to five small lesions (1-2mm),
3 = more than five small lesions or one intermediate lesion (3-4 mm),
4 = two to more intermediate lesions or one gross lesion (>4 mm),
5 = perforated ulcers.

12. THE RELATIVE AREA AND CORRESPONDING
ULCER INDEX
The total ulcerative area in
relation to the total area of each
stomach is used in determining
the ulcer index .
Relative area =
total mucosal area
total ulcerated area

13. RESULT
Ulcer was induced in 16 h fasted male wistar rats (200-250g) by oral
administration of 500 mg/kg body weight of aspirin. Seven day continuous
treatment by test drugs done before the induction of ulcer. The effect of test drugs
on different parameters are as follows.

14. EFFECT OF TEST DRUGS ON ASPIRIN INDUCED
ULCER RATS
Groups Treatment Dose Ulcer index
% Of ulcer
protection
Group - I
Administered vehicle serves
as normal control
0.2 ml 0 0
Group – II
Gastric ulcers induced by
aspirin
500 mg/kg 9.5±1.2 0
Group - III
Aspirin induced ulcer rats
treated with omeprazole
20 mg/kg 0.9±0.95 91
Group – IV
Aspirin induced ulcer rats
treated with ranitidine
300 mg/kg 2.3±0.87 76
Group – V
Aspirin induced ulcer rats
treated with prokinetic agent
40 mg/kg 4.1±0.65 57

15. CONCLUSION
Thus, the present study suggests the possible role of omeprazole is more efficient
as therapeutic agent in modifying the ulcer status and decreasing the risk factors
for ulceration than ranitidine and omeprazole – domperidone is a better option as
combination drug therapy for peptic ulcer disease (PUD).

16. REFERENCES
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Tripathi KD, essentials of medical pharmacology, jaypee brothers medical publishers (P) ltd,
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17. THANK YOU