Expt. 6 Bioassay of histamine using guinea pig ileum by matching methodVISHALJADHAV100
Objective
Principle
Requirements
Experimental specifications (conditions)
Preparation of histamine standard solution
Preparation of Tyrode solution (PSS)
Procedure
Kymograph recording of contractions
Observation table
Calculation
Result and interpretation
Expt. 8 Effect of physostigmine on DRC of acetylcholine using frog rectus abd...VISHALJADHAV100
Objective
Principle
Requirements
Experimental specifications (conditions)
Preparation of ACh and Physostigmine stock and std. solutions
Preparation of frog ringer solution (PSS)
Procedure
Kymograph recording of contractions
Observation table
Calculation of magnification value (Mf)
Graphical presentation of CRC/ DRC
Result and interpretation
Expt. 1 Introduction to in vitro pharmacology and physiological salt solutionsVISHALJADHAV100
Definitions of pharmacology & drug
Aims of experimental pharmacology
Pre-clinical pharmacology
Clinical pharmacology
Types of experiments in pharmacology
Assembly for isolated organ/ tissue related experiments
Recording (writing) levers
Physiological salt solution (PSS)
Introduction
Examples
Composition
Role of ingredients
Precautions in preparation of PSS
Selection of PSS
Expt. 7 Bioassay of acetylcholine using rat ileum by four point bioassayVISHALJADHAV100
Objective
Principle
Requirements
Experimental specifications (conditions)
Preparation of ACh stock and standard solutions
Preparation of frog ringer solution (PSS)
Procedure
Kymograph recording of contractions
Observation table
Calculation
Result and interpretation
Expt. 10 effect of spasmogens and spasmolytics using rabbit jejunumVISHALJADHAV100
Overview of Discussion
Objective
Principle
Requirements
Experimental specifications (conditions)
Drugs and solutions used in rabbit intestine experiment
Preparation of Tyrode solution (PSS)
Procedure
Kymograph recording of contractions
Observation table
Result and interpretation
Expt. 6 Bioassay of histamine using guinea pig ileum by matching methodVISHALJADHAV100
Objective
Principle
Requirements
Experimental specifications (conditions)
Preparation of histamine standard solution
Preparation of Tyrode solution (PSS)
Procedure
Kymograph recording of contractions
Observation table
Calculation
Result and interpretation
Expt. 8 Effect of physostigmine on DRC of acetylcholine using frog rectus abd...VISHALJADHAV100
Objective
Principle
Requirements
Experimental specifications (conditions)
Preparation of ACh and Physostigmine stock and std. solutions
Preparation of frog ringer solution (PSS)
Procedure
Kymograph recording of contractions
Observation table
Calculation of magnification value (Mf)
Graphical presentation of CRC/ DRC
Result and interpretation
Expt. 1 Introduction to in vitro pharmacology and physiological salt solutionsVISHALJADHAV100
Definitions of pharmacology & drug
Aims of experimental pharmacology
Pre-clinical pharmacology
Clinical pharmacology
Types of experiments in pharmacology
Assembly for isolated organ/ tissue related experiments
Recording (writing) levers
Physiological salt solution (PSS)
Introduction
Examples
Composition
Role of ingredients
Precautions in preparation of PSS
Selection of PSS
Expt. 7 Bioassay of acetylcholine using rat ileum by four point bioassayVISHALJADHAV100
Objective
Principle
Requirements
Experimental specifications (conditions)
Preparation of ACh stock and standard solutions
Preparation of frog ringer solution (PSS)
Procedure
Kymograph recording of contractions
Observation table
Calculation
Result and interpretation
Expt. 10 effect of spasmogens and spasmolytics using rabbit jejunumVISHALJADHAV100
Overview of Discussion
Objective
Principle
Requirements
Experimental specifications (conditions)
Drugs and solutions used in rabbit intestine experiment
Preparation of Tyrode solution (PSS)
Procedure
Kymograph recording of contractions
Observation table
Result and interpretation
Expt. 9 Effect of atropine on DRC of acetylcholine using rat ileumVISHALJADHAV100
Objective
Principle
Requirements
Experimental specifications (conditions)
Preparation of ACh and Atropine stock and std. solutions
Preparation of Tyrode solution (PSS)
Procedure
Kymograph recording of contractions
Observation table
Calculation
Graphical presentation of CRC/ DRC
Result and interpretation
Expt. 5 Bioassay of oxytocin using rat uterine horn by interpolation methodVISHALJADHAV100
Objective
Principle
Requirements
Experimental specifications (conditions)
Preparation of oxytocin standard solution
Preparation of De Jalon solution (PSS)
Procedure
Kymograph recording of contractions
Observation table
Graphical presentation of DRC
Calculation
Result and interpretation
Experiment 1 Introduction to In-Vitro pharmacology and physiological salt so...Kanchan Chouksey
In Vitro pharmacology studies the biological effects of a drug in an isolated environment, such as cell lines or tissues.
This setup conveniently eliminates whole organism physiological influences allowing for a detailed analysis a compound’s impact.
Heart is supplied by autonomic nervous system.
Adrenaline acts as agonist.
It acts on β-receptors and increases heart rate and amplitude.
Acetylcholine acts on muscarinic receptors as an agonist and decreases the heart rate and amplitude.
Excess concentration of KCl stops the heart beat during diastolic phase.
Ca2+ excess concentration stops the heart beat during systolic phase.
K+ and Ca2+act on cardiac muscle through non-receptor mechanism of action.
This presentation was delivered over two days to second year pharmacy students enrolled in a course in pharmacology & toxicology. This lecture is designed to accompany Goodman & Gilman's (12e) chapter 11.
Expt. 9 Effect of atropine on DRC of acetylcholine using rat ileumVISHALJADHAV100
Objective
Principle
Requirements
Experimental specifications (conditions)
Preparation of ACh and Atropine stock and std. solutions
Preparation of Tyrode solution (PSS)
Procedure
Kymograph recording of contractions
Observation table
Calculation
Graphical presentation of CRC/ DRC
Result and interpretation
Expt. 5 Bioassay of oxytocin using rat uterine horn by interpolation methodVISHALJADHAV100
Objective
Principle
Requirements
Experimental specifications (conditions)
Preparation of oxytocin standard solution
Preparation of De Jalon solution (PSS)
Procedure
Kymograph recording of contractions
Observation table
Graphical presentation of DRC
Calculation
Result and interpretation
Experiment 1 Introduction to In-Vitro pharmacology and physiological salt so...Kanchan Chouksey
In Vitro pharmacology studies the biological effects of a drug in an isolated environment, such as cell lines or tissues.
This setup conveniently eliminates whole organism physiological influences allowing for a detailed analysis a compound’s impact.
Heart is supplied by autonomic nervous system.
Adrenaline acts as agonist.
It acts on β-receptors and increases heart rate and amplitude.
Acetylcholine acts on muscarinic receptors as an agonist and decreases the heart rate and amplitude.
Excess concentration of KCl stops the heart beat during diastolic phase.
Ca2+ excess concentration stops the heart beat during systolic phase.
K+ and Ca2+act on cardiac muscle through non-receptor mechanism of action.
This presentation was delivered over two days to second year pharmacy students enrolled in a course in pharmacology & toxicology. This lecture is designed to accompany Goodman & Gilman's (12e) chapter 11.
A detailed presentation on Brain Death and Ongan transplantation.
Criteria for Brain Death are explained in detail. Legislative laws regarding the organ transplant, organ preservation are also explained.
sceening of anti arrythmic drug by apurva.pdfApurva Pawar
screening method for anti arrhythmic drugs in preclinical pharmacology. In screening rats are used and the main method Legendroff's technique, various apparatus are used like board for a animal holding, O2 cyliner etc.
Preclinical experimentation: An overviewSubhash Yende
Description of Preclinical pharmacology, laboratory animals, handling technique and route of administration, legal regulation, in vivo & in vitro experimentation
Utility value of tilt table testing in evaluationUday Prashant
I had presented in CARE Highlights session and book is being published on this topic by LAMBERT publications, Germany
http://www.google.co.in/url?sa=t&rct=j&q=&esrc=s&frm=1&source=web&cd=1&cad=rja&ved=0CCoQFjAA&url=http%3A%2F%2Fwww.amazon.in%2FEvaluation-Unexplained-Syncope-Young-Adults%2Fdp%2F3843373175&ei=lzVtUvbtCIfSrQemkYDwCg&usg=AFQjCNEK_NmIVC5j5LcLSr2hKbYFwMmRuw&sig2=okLwwgOdFiPgw4GPk7mugQ&bvm=bv.55123115,d.bmk
52 Year Old Caucasian Male Presents With A Snake Bite
SNAKEBITES
VENOM PROPERTIES
FIRST AID
Clinical Features
Systemic toxicity
Labs
Management
ANTIVENOM
DIARY OF A SNAKEBITE DEATH
A case study on Bibrachial amyotrophy, with special correlation to 'Bahusosham' (in Ayurveda) was treated for a period of 21 days . The treatment response was assessed and documented.
Read| The latest issue of The Challenger is here! We are thrilled to announce that our school paper has qualified for the NATIONAL SCHOOLS PRESS CONFERENCE (NSPC) 2024. Thank you for your unwavering support and trust. Dive into the stories that made us stand out!
Unit 8 - Information and Communication Technology (Paper I).pdfThiyagu K
This slides describes the basic concepts of ICT, basics of Email, Emerging Technology and Digital Initiatives in Education. This presentations aligns with the UGC Paper I syllabus.
The Roman Empire A Historical Colossus.pdfkaushalkr1407
The Roman Empire, a vast and enduring power, stands as one of history's most remarkable civilizations, leaving an indelible imprint on the world. It emerged from the Roman Republic, transitioning into an imperial powerhouse under the leadership of Augustus Caesar in 27 BCE. This transformation marked the beginning of an era defined by unprecedented territorial expansion, architectural marvels, and profound cultural influence.
The empire's roots lie in the city of Rome, founded, according to legend, by Romulus in 753 BCE. Over centuries, Rome evolved from a small settlement to a formidable republic, characterized by a complex political system with elected officials and checks on power. However, internal strife, class conflicts, and military ambitions paved the way for the end of the Republic. Julius Caesar’s dictatorship and subsequent assassination in 44 BCE created a power vacuum, leading to a civil war. Octavian, later Augustus, emerged victorious, heralding the Roman Empire’s birth.
Under Augustus, the empire experienced the Pax Romana, a 200-year period of relative peace and stability. Augustus reformed the military, established efficient administrative systems, and initiated grand construction projects. The empire's borders expanded, encompassing territories from Britain to Egypt and from Spain to the Euphrates. Roman legions, renowned for their discipline and engineering prowess, secured and maintained these vast territories, building roads, fortifications, and cities that facilitated control and integration.
The Roman Empire’s society was hierarchical, with a rigid class system. At the top were the patricians, wealthy elites who held significant political power. Below them were the plebeians, free citizens with limited political influence, and the vast numbers of slaves who formed the backbone of the economy. The family unit was central, governed by the paterfamilias, the male head who held absolute authority.
Culturally, the Romans were eclectic, absorbing and adapting elements from the civilizations they encountered, particularly the Greeks. Roman art, literature, and philosophy reflected this synthesis, creating a rich cultural tapestry. Latin, the Roman language, became the lingua franca of the Western world, influencing numerous modern languages.
Roman architecture and engineering achievements were monumental. They perfected the arch, vault, and dome, constructing enduring structures like the Colosseum, Pantheon, and aqueducts. These engineering marvels not only showcased Roman ingenuity but also served practical purposes, from public entertainment to water supply.
The French Revolution, which began in 1789, was a period of radical social and political upheaval in France. It marked the decline of absolute monarchies, the rise of secular and democratic republics, and the eventual rise of Napoleon Bonaparte. This revolutionary period is crucial in understanding the transition from feudalism to modernity in Europe.
For more information, visit-www.vavaclasses.com
June 3, 2024 Anti-Semitism Letter Sent to MIT President Kornbluth and MIT Cor...Levi Shapiro
Letter from the Congress of the United States regarding Anti-Semitism sent June 3rd to MIT President Sally Kornbluth, MIT Corp Chair, Mark Gorenberg
Dear Dr. Kornbluth and Mr. Gorenberg,
The US House of Representatives is deeply concerned by ongoing and pervasive acts of antisemitic
harassment and intimidation at the Massachusetts Institute of Technology (MIT). Failing to act decisively to ensure a safe learning environment for all students would be a grave dereliction of your responsibilities as President of MIT and Chair of the MIT Corporation.
This Congress will not stand idly by and allow an environment hostile to Jewish students to persist. The House believes that your institution is in violation of Title VI of the Civil Rights Act, and the inability or
unwillingness to rectify this violation through action requires accountability.
Postsecondary education is a unique opportunity for students to learn and have their ideas and beliefs challenged. However, universities receiving hundreds of millions of federal funds annually have denied
students that opportunity and have been hijacked to become venues for the promotion of terrorism, antisemitic harassment and intimidation, unlawful encampments, and in some cases, assaults and riots.
The House of Representatives will not countenance the use of federal funds to indoctrinate students into hateful, antisemitic, anti-American supporters of terrorism. Investigations into campus antisemitism by the Committee on Education and the Workforce and the Committee on Ways and Means have been expanded into a Congress-wide probe across all relevant jurisdictions to address this national crisis. The undersigned Committees will conduct oversight into the use of federal funds at MIT and its learning environment under authorities granted to each Committee.
• The Committee on Education and the Workforce has been investigating your institution since December 7, 2023. The Committee has broad jurisdiction over postsecondary education, including its compliance with Title VI of the Civil Rights Act, campus safety concerns over disruptions to the learning environment, and the awarding of federal student aid under the Higher Education Act.
• The Committee on Oversight and Accountability is investigating the sources of funding and other support flowing to groups espousing pro-Hamas propaganda and engaged in antisemitic harassment and intimidation of students. The Committee on Oversight and Accountability is the principal oversight committee of the US House of Representatives and has broad authority to investigate “any matter” at “any time” under House Rule X.
• The Committee on Ways and Means has been investigating several universities since November 15, 2023, when the Committee held a hearing entitled From Ivory Towers to Dark Corners: Investigating the Nexus Between Antisemitism, Tax-Exempt Universities, and Terror Financing. The Committee followed the hearing with letters to those institutions on January 10, 202
Acetabularia Information For Class 9 .docxvaibhavrinwa19
Acetabularia acetabulum is a single-celled green alga that in its vegetative state is morphologically differentiated into a basal rhizoid and an axially elongated stalk, which bears whorls of branching hairs. The single diploid nucleus resides in the rhizoid.
Introduction to AI for Nonprofits with Tapp NetworkTechSoup
Dive into the world of AI! Experts Jon Hill and Tareq Monaur will guide you through AI's role in enhancing nonprofit websites and basic marketing strategies, making it easy to understand and apply.
Welcome to TechSoup New Member Orientation and Q&A (May 2024).pdfTechSoup
In this webinar you will learn how your organization can access TechSoup's wide variety of product discount and donation programs. From hardware to software, we'll give you a tour of the tools available to help your nonprofit with productivity, collaboration, financial management, donor tracking, security, and more.
Palestine last event orientationfvgnh .pptxRaedMohamed3
An EFL lesson about the current events in Palestine. It is intended to be for intermediate students who wish to increase their listening skills through a short lesson in power point.
Model Attribute Check Company Auto PropertyCeline George
In Odoo, the multi-company feature allows you to manage multiple companies within a single Odoo database instance. Each company can have its own configurations while still sharing common resources such as products, customers, and suppliers.
2. AIM:
TO STUDY THE EFFECT OF VARIOUS DRUGS ON ISOLATED PERFUSED FROG’S HEART
3. Principle:
Isolated and perfused frog heart experiment has contributed to initial discoveries on
cardiovascular physiology and pharmacology. Drugs may influence rate (chronotropy) and force
(inotropy) of contraction of heart.
An increase in heart rate is called positive chronotropic and decrease in heart rate is called
negative chronotropic response.
An increase in force of contraction is positive inotropic effect and decrease is negative inotropic
response.
4. Apparatus:
Kymograph
frog board
dissection set
perfusion apparatus
(Mariotte bottle, rubber tubing and a venous cannula), iron stand, clamps, universal lever, thread,
pins, tuberculin syringe, pithing needle, frog’s ringer solution.
Experimental Animal: Frog
5.
6.
7. Parameters to be studied:
Heart Rate: This is counted by counting the number of times lever comes down per min.
Rhythm of heart: Note whether regular or irregular.
Force of contraction:It is calculated by observing the height of the tracing (amplitude). Down
going lever indicates systole. Up going lever indicates diastole.
Tone : It is the state of partial contraction of muscle at rest. This is denoted by baseline of curves.
A rise in baseline indicates increase in tone
