SlideShare a Scribd company logo
ANTIGEN AND ANTIBODY
MAN BAHADUR RANA
BPH
ACAS,NEPAL
Types of Antigen – Antibody Reaction:
• Precipitation Reaction.
• Agglutination Reaction.
• Complement Fixation.
•Neutralisation Reaction
• ELISA – Enzyme Linked ImmunoSorbent Assay.
• Immunofluorescence.
•Radioimmunoassay
The types of antigen – antibody reactions are:
Precipitation Reaction:
Antigen Added
Agglutination Reaction:
• When a particular Ag is mixed with its Ab’s in the
presence of electrolytes at a suitable temperature and
pH, the particles are clumped or agglutinated.
• The Ab of the serum causes the cellular Ag’s to form
clumps and these are called Agglutinins.
• The particulate antigens that are aggregated are
termed Agglutinogens.
 Slide agglutination: This is a rapid method to
determine the presence of agglutinating antibodies.
This test is used for blood grouping
(Haemagglutination) and cross matching.
Tube agglutination:
Tube agglutination is employed for the serological
diagnosis of typhoid, brucellosis and typhus fever.
Widal test is used for the estimation of typhoid fever.
•antitoxin + toxin  neutralization
•toxigenecity test
•Shick test
•Nagler reaction
Neutralisation Reaction
Complement Fixation:
•This complement system 11 proteins
•Fc component of Ag-Ab complex.
•E.g wasserman reaction
•Immobilisation test for T. pallidium
 Radioimmunoassay (RIA)  in vitro assay  concentrations
of antigens (for example, hormone levels in theblood) by use of
antibodies.
Principle:
RIA relies on the radioactivity of a specific isotope labelled antibody or
antigen
Application
circulation proteins, hormones and drugs
INTRODUCTION
12
Solid phase radioimmunoassay
13
Immunofluorescence utilizes fluorescent-
labeled antibodies to detect specific target
antigens. It is used widely in both scientific
research and clinical laboratories.
Coons et al (1942) labeled dyes can be conjugated
to Ab’s and these labeled antibodies can be used to
detect Ag’s.
•Fluorescein, Phycoerythrin.
Immunofluorescence:
INTRODUCTION TO ELISA
 ELISA, or enzyme-linked immunosorbent
assay, quantitative immunological Ag- Ab
reaction is monitored by enzyme
measurements.
16
Antigen  antibody for bph class

More Related Content

What's hot

Immunoprecipitation
ImmunoprecipitationImmunoprecipitation
Immunoprecipitation
Bob Bin
 
Antigen antibody reaction (part: 2)
Antigen antibody reaction (part: 2)Antigen antibody reaction (part: 2)
Antigen antibody reaction (part: 2)
Dr. Nimra Khan
 
Antigen - antibody reaction based on medical science
Antigen - antibody reaction based on medical scienceAntigen - antibody reaction based on medical science
Antigen - antibody reaction based on medical science
KAVIN6369950450
 
Immunotechniques ppt hamsa
Immunotechniques ppt hamsaImmunotechniques ppt hamsa
Immunotechniques ppt hamsa
Hamsa Jacy
 
Ag ab rxns
Ag ab rxnsAg ab rxns
Ag ab rxns
KAVI PRIYA
 
Antigen Antibody techniques 6 lecture
Antigen Antibody techniques 6 lectureAntigen Antibody techniques 6 lecture
Antigen Antibody techniques 6 lecture
the university of lahore
 
Serological tests ppt2
Serological tests ppt2Serological tests ppt2
Serological tests ppt2
Steph Chitsulo
 
8 ag ab reactions07
8 ag ab reactions078 ag ab reactions07
8 ag ab reactions07
Bruno Mmassy
 
Antigen and antibody reaction
Antigen and antibody reactionAntigen and antibody reaction
Antigen and antibody reaction
Rachana Tiwari
 
Serological techniques and immune assays
Serological techniques and immune assaysSerological techniques and immune assays
Serological techniques and immune assays
eman youssif
 
Precipitation reaction mahadi ppt
Precipitation reaction mahadi pptPrecipitation reaction mahadi ppt
Precipitation reaction mahadi ppt
Dido Medo
 
immunology
immunologyimmunology
immunology
research
 
Antigen antibody interaction.pptx jitu
Antigen antibody interaction.pptx jituAntigen antibody interaction.pptx jitu
Antigen antibody interaction.pptx jitu
JItupalKalita
 
2. antigen antibody reaction
2. antigen antibody reaction2. antigen antibody reaction
2. antigen antibody reaction
Saugat Chapagain
 
Antigen antibody
Antigen antibodyAntigen antibody
Antigen antibody
Bruno Mmassy
 
antigen antibody reactions
antigen antibody reactionsantigen antibody reactions
ANTIGEN-ANTIBODY REACTION (in vitro)
ANTIGEN-ANTIBODY REACTION (in vitro)ANTIGEN-ANTIBODY REACTION (in vitro)
ANTIGEN-ANTIBODY REACTION (in vitro)
NITISH SHAH
 
Antigen antibody reaction mahadi ppt
Antigen antibody reaction mahadi pptAntigen antibody reaction mahadi ppt
Antigen antibody reaction mahadi ppt
Dido Medo
 
Antigen –antibody reaction
Antigen –antibody reactionAntigen –antibody reaction
Antigen –antibody reaction
hitech medical college
 
antigen antibody interaction
antigen antibody interactionantigen antibody interaction
antigen antibody interaction
Arunima Sur
 

What's hot (20)

Immunoprecipitation
ImmunoprecipitationImmunoprecipitation
Immunoprecipitation
 
Antigen antibody reaction (part: 2)
Antigen antibody reaction (part: 2)Antigen antibody reaction (part: 2)
Antigen antibody reaction (part: 2)
 
Antigen - antibody reaction based on medical science
Antigen - antibody reaction based on medical scienceAntigen - antibody reaction based on medical science
Antigen - antibody reaction based on medical science
 
Immunotechniques ppt hamsa
Immunotechniques ppt hamsaImmunotechniques ppt hamsa
Immunotechniques ppt hamsa
 
Ag ab rxns
Ag ab rxnsAg ab rxns
Ag ab rxns
 
Antigen Antibody techniques 6 lecture
Antigen Antibody techniques 6 lectureAntigen Antibody techniques 6 lecture
Antigen Antibody techniques 6 lecture
 
Serological tests ppt2
Serological tests ppt2Serological tests ppt2
Serological tests ppt2
 
8 ag ab reactions07
8 ag ab reactions078 ag ab reactions07
8 ag ab reactions07
 
Antigen and antibody reaction
Antigen and antibody reactionAntigen and antibody reaction
Antigen and antibody reaction
 
Serological techniques and immune assays
Serological techniques and immune assaysSerological techniques and immune assays
Serological techniques and immune assays
 
Precipitation reaction mahadi ppt
Precipitation reaction mahadi pptPrecipitation reaction mahadi ppt
Precipitation reaction mahadi ppt
 
immunology
immunologyimmunology
immunology
 
Antigen antibody interaction.pptx jitu
Antigen antibody interaction.pptx jituAntigen antibody interaction.pptx jitu
Antigen antibody interaction.pptx jitu
 
2. antigen antibody reaction
2. antigen antibody reaction2. antigen antibody reaction
2. antigen antibody reaction
 
Antigen antibody
Antigen antibodyAntigen antibody
Antigen antibody
 
antigen antibody reactions
antigen antibody reactionsantigen antibody reactions
antigen antibody reactions
 
ANTIGEN-ANTIBODY REACTION (in vitro)
ANTIGEN-ANTIBODY REACTION (in vitro)ANTIGEN-ANTIBODY REACTION (in vitro)
ANTIGEN-ANTIBODY REACTION (in vitro)
 
Antigen antibody reaction mahadi ppt
Antigen antibody reaction mahadi pptAntigen antibody reaction mahadi ppt
Antigen antibody reaction mahadi ppt
 
Antigen –antibody reaction
Antigen –antibody reactionAntigen –antibody reaction
Antigen –antibody reaction
 
antigen antibody interaction
antigen antibody interactionantigen antibody interaction
antigen antibody interaction
 

Similar to Antigen antibody for bph class

Methods for detecting antigens and antibodies
Methods for detecting antigens and antibodiesMethods for detecting antigens and antibodies
Methods for detecting antigens and antibodies
Amjad Afridi
 
Antigen antibody reactions
Antigen antibody reactions Antigen antibody reactions
Antigen antibody reactions
Dr. Aamir Ali Khan
 
antigenandantibodyreaction-120515041533-phpapp01.pdf
antigenandantibodyreaction-120515041533-phpapp01.pdfantigenandantibodyreaction-120515041533-phpapp01.pdf
antigenandantibodyreaction-120515041533-phpapp01.pdf
Monish Pokra
 
Antigen antibody interaction .pptx 1
Antigen antibody interaction .pptx 1Antigen antibody interaction .pptx 1
Antigen antibody interaction .pptx 1
Vipin Shukla
 
Ag -Ab reactions 1.ppt
Ag -Ab reactions 1.pptAg -Ab reactions 1.ppt
Ag -Ab reactions 1.ppt
dipinbhandari
 
Antibody_Antigen_Reactions.pptx antigen antibody reactions
Antibody_Antigen_Reactions.pptx antigen antibody reactionsAntibody_Antigen_Reactions.pptx antigen antibody reactions
Antibody_Antigen_Reactions.pptx antigen antibody reactions
usamabinaltafaltafhu
 
Antigen Antibody Reactions.pptx
Antigen Antibody Reactions.pptxAntigen Antibody Reactions.pptx
Antigen Antibody Reactions.pptx
Dr. Rakesh Prasad Sah
 
Agglutination
AgglutinationAgglutination
Agglutination
Monisha Jayabalan
 
Agglutination test ,antigen antibody reaction
Agglutination test ,antigen antibody reaction  Agglutination test ,antigen antibody reaction
Agglutination test ,antigen antibody reaction
JF institute of health sciences
 
Ag-Ab Reactions
Ag-Ab ReactionsAg-Ab Reactions
Ag-Ab Reactions
Pulipati Sowjanya
 
Practical immunology.
Practical immunology.Practical immunology.
Practical immunology.
امجد معوض
 
Agglutination test class 5.docx.pdf
Agglutination test class 5.docx.pdfAgglutination test class 5.docx.pdf
Agglutination test class 5.docx.pdf
mustafahamza11
 
Antigen antibody reactions
Antigen antibody reactionsAntigen antibody reactions
Antigen antibody reactions
Dr NEETHU ASOKAN
 
Antigen antibody interaction
Antigen antibody interactionAntigen antibody interaction
Antigen antibody interaction
Pranab Debbarma
 
Ag ab i (dwd)
Ag ab i (dwd)Ag ab i (dwd)
Ag ab i (dwd)
deepak deshkar
 
Ag Ab I (Dwd)
Ag Ab I (Dwd)Ag Ab I (Dwd)
Ag Ab I (Dwd)
deepak deshkar
 
Serological tests
Serological testsSerological tests
Serological tests
jessicaeo
 
Serological tests
Serological testsSerological tests
Serological tests
dream10f
 
ELISA.pptx
ELISA.pptxELISA.pptx
ELISA.pptx
Rooban Sivakumar
 
Serological reactions by Dr. Himanshu Khatri
Serological reactions by Dr. Himanshu KhatriSerological reactions by Dr. Himanshu Khatri
Serological reactions by Dr. Himanshu Khatri
DrHimanshuKhatri
 

Similar to Antigen antibody for bph class (20)

Methods for detecting antigens and antibodies
Methods for detecting antigens and antibodiesMethods for detecting antigens and antibodies
Methods for detecting antigens and antibodies
 
Antigen antibody reactions
Antigen antibody reactions Antigen antibody reactions
Antigen antibody reactions
 
antigenandantibodyreaction-120515041533-phpapp01.pdf
antigenandantibodyreaction-120515041533-phpapp01.pdfantigenandantibodyreaction-120515041533-phpapp01.pdf
antigenandantibodyreaction-120515041533-phpapp01.pdf
 
Antigen antibody interaction .pptx 1
Antigen antibody interaction .pptx 1Antigen antibody interaction .pptx 1
Antigen antibody interaction .pptx 1
 
Ag -Ab reactions 1.ppt
Ag -Ab reactions 1.pptAg -Ab reactions 1.ppt
Ag -Ab reactions 1.ppt
 
Antibody_Antigen_Reactions.pptx antigen antibody reactions
Antibody_Antigen_Reactions.pptx antigen antibody reactionsAntibody_Antigen_Reactions.pptx antigen antibody reactions
Antibody_Antigen_Reactions.pptx antigen antibody reactions
 
Antigen Antibody Reactions.pptx
Antigen Antibody Reactions.pptxAntigen Antibody Reactions.pptx
Antigen Antibody Reactions.pptx
 
Agglutination
AgglutinationAgglutination
Agglutination
 
Agglutination test ,antigen antibody reaction
Agglutination test ,antigen antibody reaction  Agglutination test ,antigen antibody reaction
Agglutination test ,antigen antibody reaction
 
Ag-Ab Reactions
Ag-Ab ReactionsAg-Ab Reactions
Ag-Ab Reactions
 
Practical immunology.
Practical immunology.Practical immunology.
Practical immunology.
 
Agglutination test class 5.docx.pdf
Agglutination test class 5.docx.pdfAgglutination test class 5.docx.pdf
Agglutination test class 5.docx.pdf
 
Antigen antibody reactions
Antigen antibody reactionsAntigen antibody reactions
Antigen antibody reactions
 
Antigen antibody interaction
Antigen antibody interactionAntigen antibody interaction
Antigen antibody interaction
 
Ag ab i (dwd)
Ag ab i (dwd)Ag ab i (dwd)
Ag ab i (dwd)
 
Ag Ab I (Dwd)
Ag Ab I (Dwd)Ag Ab I (Dwd)
Ag Ab I (Dwd)
 
Serological tests
Serological testsSerological tests
Serological tests
 
Serological tests
Serological testsSerological tests
Serological tests
 
ELISA.pptx
ELISA.pptxELISA.pptx
ELISA.pptx
 
Serological reactions by Dr. Himanshu Khatri
Serological reactions by Dr. Himanshu KhatriSerological reactions by Dr. Himanshu Khatri
Serological reactions by Dr. Himanshu Khatri
 

More from RaNa MB

Water soluble vitamis for bph
Water soluble vitamis for bph Water soluble vitamis for bph
Water soluble vitamis for bph
RaNa MB
 
Vitamin fat soluble bph class
Vitamin fat soluble bph classVitamin fat soluble bph class
Vitamin fat soluble bph class
RaNa MB
 
Vaccine and vaccination. for bph class
Vaccine and vaccination. for bph class Vaccine and vaccination. for bph class
Vaccine and vaccination. for bph class
RaNa MB
 
Protein bph
Protein bphProtein bph
Protein bph
RaNa MB
 
Nucleic acid bph
Nucleic acid bph Nucleic acid bph
Nucleic acid bph
RaNa MB
 
Notes introduction to biochemistry
Notes introduction to biochemistryNotes introduction to biochemistry
Notes introduction to biochemistry
RaNa MB
 
Minerals FOR BPH
Minerals FOR BPHMinerals FOR BPH
Minerals FOR BPH
RaNa MB
 
Lipids bph
Lipids bph Lipids bph
Lipids bph
RaNa MB
 
Lipids BPH
Lipids BPHLipids BPH
Lipids BPH
RaNa MB
 
Introduction of immunology for bph class
Introduction of immunology for bph classIntroduction of immunology for bph class
Introduction of immunology for bph class
RaNa MB
 
Infection and its mechanism
Infection and its mechanismInfection and its mechanism
Infection and its mechanism
RaNa MB
 
Immunity, immune def, hypersensitivity
Immunity, immune def, hypersensitivityImmunity, immune def, hypersensitivity
Immunity, immune def, hypersensitivity
RaNa MB
 
Hypersensitivity reaction, bph
Hypersensitivity reaction, bph Hypersensitivity reaction, bph
Hypersensitivity reaction, bph
RaNa MB
 
Enzymes bph
Enzymes bphEnzymes bph
Enzymes bph
RaNa MB
 
Defence mechanisms. bph
Defence mechanisms. bphDefence mechanisms. bph
Defence mechanisms. bph
RaNa MB
 
Carbohydrate for BPH
Carbohydrate for BPHCarbohydrate for BPH
Carbohydrate for BPH
RaNa MB
 
Buffer system for bph
Buffer system for bphBuffer system for bph
Buffer system for bph
RaNa MB
 
Hepatovirusfor microbiology student
Hepatovirusfor microbiology studentHepatovirusfor microbiology student
Hepatovirusfor microbiology student
RaNa MB
 
Hepatitis b virus
Hepatitis b virusHepatitis b virus
Hepatitis b virus
RaNa MB
 
Harmfulandusefulofmicroorganism 111203044208-phpapp02
Harmfulandusefulofmicroorganism 111203044208-phpapp02Harmfulandusefulofmicroorganism 111203044208-phpapp02
Harmfulandusefulofmicroorganism 111203044208-phpapp02
RaNa MB
 

More from RaNa MB (20)

Water soluble vitamis for bph
Water soluble vitamis for bph Water soluble vitamis for bph
Water soluble vitamis for bph
 
Vitamin fat soluble bph class
Vitamin fat soluble bph classVitamin fat soluble bph class
Vitamin fat soluble bph class
 
Vaccine and vaccination. for bph class
Vaccine and vaccination. for bph class Vaccine and vaccination. for bph class
Vaccine and vaccination. for bph class
 
Protein bph
Protein bphProtein bph
Protein bph
 
Nucleic acid bph
Nucleic acid bph Nucleic acid bph
Nucleic acid bph
 
Notes introduction to biochemistry
Notes introduction to biochemistryNotes introduction to biochemistry
Notes introduction to biochemistry
 
Minerals FOR BPH
Minerals FOR BPHMinerals FOR BPH
Minerals FOR BPH
 
Lipids bph
Lipids bph Lipids bph
Lipids bph
 
Lipids BPH
Lipids BPHLipids BPH
Lipids BPH
 
Introduction of immunology for bph class
Introduction of immunology for bph classIntroduction of immunology for bph class
Introduction of immunology for bph class
 
Infection and its mechanism
Infection and its mechanismInfection and its mechanism
Infection and its mechanism
 
Immunity, immune def, hypersensitivity
Immunity, immune def, hypersensitivityImmunity, immune def, hypersensitivity
Immunity, immune def, hypersensitivity
 
Hypersensitivity reaction, bph
Hypersensitivity reaction, bph Hypersensitivity reaction, bph
Hypersensitivity reaction, bph
 
Enzymes bph
Enzymes bphEnzymes bph
Enzymes bph
 
Defence mechanisms. bph
Defence mechanisms. bphDefence mechanisms. bph
Defence mechanisms. bph
 
Carbohydrate for BPH
Carbohydrate for BPHCarbohydrate for BPH
Carbohydrate for BPH
 
Buffer system for bph
Buffer system for bphBuffer system for bph
Buffer system for bph
 
Hepatovirusfor microbiology student
Hepatovirusfor microbiology studentHepatovirusfor microbiology student
Hepatovirusfor microbiology student
 
Hepatitis b virus
Hepatitis b virusHepatitis b virus
Hepatitis b virus
 
Harmfulandusefulofmicroorganism 111203044208-phpapp02
Harmfulandusefulofmicroorganism 111203044208-phpapp02Harmfulandusefulofmicroorganism 111203044208-phpapp02
Harmfulandusefulofmicroorganism 111203044208-phpapp02
 

Recently uploaded

MARY JANE WILSON, A “BOA MÃE” .
MARY JANE WILSON, A “BOA MÃE”           .MARY JANE WILSON, A “BOA MÃE”           .
MARY JANE WILSON, A “BOA MÃE” .
Colégio Santa Teresinha
 
How to Build a Module in Odoo 17 Using the Scaffold Method
How to Build a Module in Odoo 17 Using the Scaffold MethodHow to Build a Module in Odoo 17 Using the Scaffold Method
How to Build a Module in Odoo 17 Using the Scaffold Method
Celine George
 
Walmart Business+ and Spark Good for Nonprofits.pdf
Walmart Business+ and Spark Good for Nonprofits.pdfWalmart Business+ and Spark Good for Nonprofits.pdf
Walmart Business+ and Spark Good for Nonprofits.pdf
TechSoup
 
বাংলাদেশ অর্থনৈতিক সমীক্ষা (Economic Review) ২০২৪ UJS App.pdf
বাংলাদেশ অর্থনৈতিক সমীক্ষা (Economic Review) ২০২৪ UJS App.pdfবাংলাদেশ অর্থনৈতিক সমীক্ষা (Economic Review) ২০২৪ UJS App.pdf
বাংলাদেশ অর্থনৈতিক সমীক্ষা (Economic Review) ২০২৪ UJS App.pdf
eBook.com.bd (প্রয়োজনীয় বাংলা বই)
 
RPMS TEMPLATE FOR SCHOOL YEAR 2023-2024 FOR TEACHER 1 TO TEACHER 3
RPMS TEMPLATE FOR SCHOOL YEAR 2023-2024 FOR TEACHER 1 TO TEACHER 3RPMS TEMPLATE FOR SCHOOL YEAR 2023-2024 FOR TEACHER 1 TO TEACHER 3
RPMS TEMPLATE FOR SCHOOL YEAR 2023-2024 FOR TEACHER 1 TO TEACHER 3
IreneSebastianRueco1
 
Azure Interview Questions and Answers PDF By ScholarHat
Azure Interview Questions and Answers PDF By ScholarHatAzure Interview Questions and Answers PDF By ScholarHat
Azure Interview Questions and Answers PDF By ScholarHat
Scholarhat
 
South African Journal of Science: Writing with integrity workshop (2024)
South African Journal of Science: Writing with integrity workshop (2024)South African Journal of Science: Writing with integrity workshop (2024)
South African Journal of Science: Writing with integrity workshop (2024)
Academy of Science of South Africa
 
How to Setup Warehouse & Location in Odoo 17 Inventory
How to Setup Warehouse & Location in Odoo 17 InventoryHow to Setup Warehouse & Location in Odoo 17 Inventory
How to Setup Warehouse & Location in Odoo 17 Inventory
Celine George
 
The History of Stoke Newington Street Names
The History of Stoke Newington Street NamesThe History of Stoke Newington Street Names
The History of Stoke Newington Street Names
History of Stoke Newington
 
Natural birth techniques - Mrs.Akanksha Trivedi Rama University
Natural birth techniques - Mrs.Akanksha Trivedi Rama UniversityNatural birth techniques - Mrs.Akanksha Trivedi Rama University
Natural birth techniques - Mrs.Akanksha Trivedi Rama University
Akanksha trivedi rama nursing college kanpur.
 
BBR 2024 Summer Sessions Interview Training
BBR  2024 Summer Sessions Interview TrainingBBR  2024 Summer Sessions Interview Training
BBR 2024 Summer Sessions Interview Training
Katrina Pritchard
 
clinical examination of hip joint (1).pdf
clinical examination of hip joint (1).pdfclinical examination of hip joint (1).pdf
clinical examination of hip joint (1).pdf
Priyankaranawat4
 
C1 Rubenstein AP HuG xxxxxxxxxxxxxx.pptx
C1 Rubenstein AP HuG xxxxxxxxxxxxxx.pptxC1 Rubenstein AP HuG xxxxxxxxxxxxxx.pptx
C1 Rubenstein AP HuG xxxxxxxxxxxxxx.pptx
mulvey2
 
writing about opinions about Australia the movie
writing about opinions about Australia the moviewriting about opinions about Australia the movie
writing about opinions about Australia the movie
Nicholas Montgomery
 
How to Fix the Import Error in the Odoo 17
How to Fix the Import Error in the Odoo 17How to Fix the Import Error in the Odoo 17
How to Fix the Import Error in the Odoo 17
Celine George
 
World environment day ppt For 5 June 2024
World environment day ppt For 5 June 2024World environment day ppt For 5 June 2024
World environment day ppt For 5 June 2024
ak6969907
 
Advanced Java[Extra Concepts, Not Difficult].docx
Advanced Java[Extra Concepts, Not Difficult].docxAdvanced Java[Extra Concepts, Not Difficult].docx
Advanced Java[Extra Concepts, Not Difficult].docx
adhitya5119
 
ANATOMY AND BIOMECHANICS OF HIP JOINT.pdf
ANATOMY AND BIOMECHANICS OF HIP JOINT.pdfANATOMY AND BIOMECHANICS OF HIP JOINT.pdf
ANATOMY AND BIOMECHANICS OF HIP JOINT.pdf
Priyankaranawat4
 
What is Digital Literacy? A guest blog from Andy McLaughlin, University of Ab...
What is Digital Literacy? A guest blog from Andy McLaughlin, University of Ab...What is Digital Literacy? A guest blog from Andy McLaughlin, University of Ab...
What is Digital Literacy? A guest blog from Andy McLaughlin, University of Ab...
GeorgeMilliken2
 
S1-Introduction-Biopesticides in ICM.pptx
S1-Introduction-Biopesticides in ICM.pptxS1-Introduction-Biopesticides in ICM.pptx
S1-Introduction-Biopesticides in ICM.pptx
tarandeep35
 

Recently uploaded (20)

MARY JANE WILSON, A “BOA MÃE” .
MARY JANE WILSON, A “BOA MÃE”           .MARY JANE WILSON, A “BOA MÃE”           .
MARY JANE WILSON, A “BOA MÃE” .
 
How to Build a Module in Odoo 17 Using the Scaffold Method
How to Build a Module in Odoo 17 Using the Scaffold MethodHow to Build a Module in Odoo 17 Using the Scaffold Method
How to Build a Module in Odoo 17 Using the Scaffold Method
 
Walmart Business+ and Spark Good for Nonprofits.pdf
Walmart Business+ and Spark Good for Nonprofits.pdfWalmart Business+ and Spark Good for Nonprofits.pdf
Walmart Business+ and Spark Good for Nonprofits.pdf
 
বাংলাদেশ অর্থনৈতিক সমীক্ষা (Economic Review) ২০২৪ UJS App.pdf
বাংলাদেশ অর্থনৈতিক সমীক্ষা (Economic Review) ২০২৪ UJS App.pdfবাংলাদেশ অর্থনৈতিক সমীক্ষা (Economic Review) ২০২৪ UJS App.pdf
বাংলাদেশ অর্থনৈতিক সমীক্ষা (Economic Review) ২০২৪ UJS App.pdf
 
RPMS TEMPLATE FOR SCHOOL YEAR 2023-2024 FOR TEACHER 1 TO TEACHER 3
RPMS TEMPLATE FOR SCHOOL YEAR 2023-2024 FOR TEACHER 1 TO TEACHER 3RPMS TEMPLATE FOR SCHOOL YEAR 2023-2024 FOR TEACHER 1 TO TEACHER 3
RPMS TEMPLATE FOR SCHOOL YEAR 2023-2024 FOR TEACHER 1 TO TEACHER 3
 
Azure Interview Questions and Answers PDF By ScholarHat
Azure Interview Questions and Answers PDF By ScholarHatAzure Interview Questions and Answers PDF By ScholarHat
Azure Interview Questions and Answers PDF By ScholarHat
 
South African Journal of Science: Writing with integrity workshop (2024)
South African Journal of Science: Writing with integrity workshop (2024)South African Journal of Science: Writing with integrity workshop (2024)
South African Journal of Science: Writing with integrity workshop (2024)
 
How to Setup Warehouse & Location in Odoo 17 Inventory
How to Setup Warehouse & Location in Odoo 17 InventoryHow to Setup Warehouse & Location in Odoo 17 Inventory
How to Setup Warehouse & Location in Odoo 17 Inventory
 
The History of Stoke Newington Street Names
The History of Stoke Newington Street NamesThe History of Stoke Newington Street Names
The History of Stoke Newington Street Names
 
Natural birth techniques - Mrs.Akanksha Trivedi Rama University
Natural birth techniques - Mrs.Akanksha Trivedi Rama UniversityNatural birth techniques - Mrs.Akanksha Trivedi Rama University
Natural birth techniques - Mrs.Akanksha Trivedi Rama University
 
BBR 2024 Summer Sessions Interview Training
BBR  2024 Summer Sessions Interview TrainingBBR  2024 Summer Sessions Interview Training
BBR 2024 Summer Sessions Interview Training
 
clinical examination of hip joint (1).pdf
clinical examination of hip joint (1).pdfclinical examination of hip joint (1).pdf
clinical examination of hip joint (1).pdf
 
C1 Rubenstein AP HuG xxxxxxxxxxxxxx.pptx
C1 Rubenstein AP HuG xxxxxxxxxxxxxx.pptxC1 Rubenstein AP HuG xxxxxxxxxxxxxx.pptx
C1 Rubenstein AP HuG xxxxxxxxxxxxxx.pptx
 
writing about opinions about Australia the movie
writing about opinions about Australia the moviewriting about opinions about Australia the movie
writing about opinions about Australia the movie
 
How to Fix the Import Error in the Odoo 17
How to Fix the Import Error in the Odoo 17How to Fix the Import Error in the Odoo 17
How to Fix the Import Error in the Odoo 17
 
World environment day ppt For 5 June 2024
World environment day ppt For 5 June 2024World environment day ppt For 5 June 2024
World environment day ppt For 5 June 2024
 
Advanced Java[Extra Concepts, Not Difficult].docx
Advanced Java[Extra Concepts, Not Difficult].docxAdvanced Java[Extra Concepts, Not Difficult].docx
Advanced Java[Extra Concepts, Not Difficult].docx
 
ANATOMY AND BIOMECHANICS OF HIP JOINT.pdf
ANATOMY AND BIOMECHANICS OF HIP JOINT.pdfANATOMY AND BIOMECHANICS OF HIP JOINT.pdf
ANATOMY AND BIOMECHANICS OF HIP JOINT.pdf
 
What is Digital Literacy? A guest blog from Andy McLaughlin, University of Ab...
What is Digital Literacy? A guest blog from Andy McLaughlin, University of Ab...What is Digital Literacy? A guest blog from Andy McLaughlin, University of Ab...
What is Digital Literacy? A guest blog from Andy McLaughlin, University of Ab...
 
S1-Introduction-Biopesticides in ICM.pptx
S1-Introduction-Biopesticides in ICM.pptxS1-Introduction-Biopesticides in ICM.pptx
S1-Introduction-Biopesticides in ICM.pptx
 

Antigen antibody for bph class

  • 1. ANTIGEN AND ANTIBODY MAN BAHADUR RANA BPH ACAS,NEPAL
  • 2.
  • 3. Types of Antigen – Antibody Reaction: • Precipitation Reaction. • Agglutination Reaction. • Complement Fixation. •Neutralisation Reaction • ELISA – Enzyme Linked ImmunoSorbent Assay. • Immunofluorescence. •Radioimmunoassay The types of antigen – antibody reactions are:
  • 6.
  • 7. Agglutination Reaction: • When a particular Ag is mixed with its Ab’s in the presence of electrolytes at a suitable temperature and pH, the particles are clumped or agglutinated. • The Ab of the serum causes the cellular Ag’s to form clumps and these are called Agglutinins. • The particulate antigens that are aggregated are termed Agglutinogens.  Slide agglutination: This is a rapid method to determine the presence of agglutinating antibodies. This test is used for blood grouping (Haemagglutination) and cross matching.
  • 8. Tube agglutination: Tube agglutination is employed for the serological diagnosis of typhoid, brucellosis and typhus fever. Widal test is used for the estimation of typhoid fever.
  • 9. •antitoxin + toxin  neutralization •toxigenecity test •Shick test •Nagler reaction Neutralisation Reaction
  • 10. Complement Fixation: •This complement system 11 proteins •Fc component of Ag-Ab complex. •E.g wasserman reaction •Immobilisation test for T. pallidium
  • 11.
  • 12.  Radioimmunoassay (RIA)  in vitro assay  concentrations of antigens (for example, hormone levels in theblood) by use of antibodies. Principle: RIA relies on the radioactivity of a specific isotope labelled antibody or antigen Application circulation proteins, hormones and drugs INTRODUCTION 12
  • 14. Immunofluorescence utilizes fluorescent- labeled antibodies to detect specific target antigens. It is used widely in both scientific research and clinical laboratories. Coons et al (1942) labeled dyes can be conjugated to Ab’s and these labeled antibodies can be used to detect Ag’s. •Fluorescein, Phycoerythrin. Immunofluorescence:
  • 15.
  • 16. INTRODUCTION TO ELISA  ELISA, or enzyme-linked immunosorbent assay, quantitative immunological Ag- Ab reaction is monitored by enzyme measurements. 16