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Antibodies as Drugs Lauren Lahey April 26, 2007
Overview ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Emerging Themes ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
At the turn of the 20 th  century: ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
More recently: ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Important Terms ,[object Object],[object Object],[object Object],[object Object],[object Object]
The Structure of an Antibody ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Formation of Disulfide Bonds ,[object Object],[object Object],[object Object],[object Object]
Compliment fixation First antibody secreted in development μ IgM Promotes antibody-dependent cellular cytotoxicity (ADCC)  Compliment fixation Main antibody in serum Most Stable γ IgG Cells that secrete histamines ε IgE B Cell surface receptor δ IgD External Secretions or α IgA Biological Activity Distribution Diagram Heavy Chain Immunoglobulin   (Ig) Class
Hypervariable Loops  ,[object Object],[object Object],[object Object],[object Object],[object Object]
A Dynamic Binding Site ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Antibody Pharmokinetics ,[object Object],[object Object],[object Object],[object Object],[object Object]
Cross-linking ,[object Object],[object Object],[object Object],[object Object]
Mechanisms of Action ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
“Humanizing” Antibodies ,[object Object],[object Object],[object Object],[object Object],[object Object]
Production of Human Antibodies
Pharmaceutical Antibodies ,[object Object],[object Object],[object Object],[object Object],[object Object]
Nomenclature of Monoclonal Antibodies toxin as target -tox(a)- nervous system -neu(r)- miscellaneous tumor -tu(m)- prostate tumor -pr(o)- ovarian tumor -go(v)- testicular tumor -go(t)- mammary tumor -ma(r)- melanoma -me(l)- colonic tumor -co(l)- rat/murine hybrid -axo- interleukin -ki(n)- humanized -zu- musculoskeletal -mu(l)- chimeric -xi- cardiovascular -ci(r)- primate -i- infectious lesions -le(s)- hamster -e- immune -li(m)- rat -a- bacterial -ba(c)- mouse -o- viral -vi(r)- -mab human -u- bone -o(s)- variable Suffix Source Target Prefix
Autoimmune Disease ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Rheumatoid Arthritis ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Infliximab ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Adalimumab ,[object Object],[object Object],[object Object],[object Object],[object Object]
Rituximab ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Interesting Variations ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Nanobodies ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
References ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]

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Antibodies as Drugs

  • 1. Antibodies as Drugs Lauren Lahey April 26, 2007
  • 2.
  • 3.
  • 4.
  • 5.
  • 6.
  • 7.
  • 8.
  • 9. Compliment fixation First antibody secreted in development μ IgM Promotes antibody-dependent cellular cytotoxicity (ADCC) Compliment fixation Main antibody in serum Most Stable γ IgG Cells that secrete histamines ε IgE B Cell surface receptor δ IgD External Secretions or α IgA Biological Activity Distribution Diagram Heavy Chain Immunoglobulin (Ig) Class
  • 10.
  • 11.
  • 12.
  • 13.
  • 14.
  • 15.
  • 16. Production of Human Antibodies
  • 17.
  • 18. Nomenclature of Monoclonal Antibodies toxin as target -tox(a)- nervous system -neu(r)- miscellaneous tumor -tu(m)- prostate tumor -pr(o)- ovarian tumor -go(v)- testicular tumor -go(t)- mammary tumor -ma(r)- melanoma -me(l)- colonic tumor -co(l)- rat/murine hybrid -axo- interleukin -ki(n)- humanized -zu- musculoskeletal -mu(l)- chimeric -xi- cardiovascular -ci(r)- primate -i- infectious lesions -le(s)- hamster -e- immune -li(m)- rat -a- bacterial -ba(c)- mouse -o- viral -vi(r)- -mab human -u- bone -o(s)- variable Suffix Source Target Prefix
  • 19.
  • 20.
  • 21.
  • 22.
  • 23.
  • 24.
  • 25.
  • 26.