The document discusses nuclear magnetic resonance (NMR) spectroscopy, which exploits the magnetic properties of atomic nuclei. It explains basic NMR techniques, how NMR relies on nuclear magnetic resonance, and its importance in providing structural information about molecules. The document also discusses chemical shifts, spin-spin coupling, NMR analysis applications including quantitative NMR and solid state NMR, and the history and some websites related to NMR.

1. Cairo university
Faculty of pharmacy
Analytical chemistry
department

2. NMR Spectroscopy

3. Explaining NMR
UV/Vis spectroscopy
Sample

4. Nuclear magnetic resonance
spectroscopy
• Def. :-known as NMR spectroscopy, is a
research technique that exploits the
magnetic properties of certain a certain
atomic nuclei.
• . It determines the physical and chemical
properties of atoms in which they are
contained.

5. •Basic NMR techniques:-
1-The NMR sample is prepared in a
thin-walled glass tube - a NMR tube.
2-When placed in a magnetic field,
NMR active nuclei (such as 1H
or 13C) absorb electromagnetic
radiation .

6. • Now we have to answer an important
question, on which phenomenon it relies ?
• As an answer for this question we will ay it
depends on nuclear magnetic resonance.
• Importance :-
1-Can provide detailed information about the
structure, dynamics, reaction state, and
chemical environment of molecules.
2-is used by chemists and biochemists to
investigate the properties of organic
molecules at a frequency characteristic of
the isotope.

7. •Now it is time to talk about the chemical
shift.
• What is chemical shift?
•Chemical shift:-is the resonant frequency of a
nucleus relative to a standard, this standard
is often tetramethylsilane, Si(CH3)4,
abbreviated TMS.

8. Example of the chemical shift:
• NMR spectrum of hexaborane
B6H10 showing peaks shifted in
frequency, which give clues as to the
molecular structure.
• Importance :-The chemical shift provides
information about the structure of the
molecule.

9. • Do you remember spin-spin coupling?
• It a special type of it. This coupling arises
from the interaction of different spin states
through the chemical bonds of a molecule
and results in the splitting of NMR signals.
• Importance:-it provides detailed insight
into the connectivity of atoms in a
molecule.

10. NMR analysis applications
1-Chemical structure analysis of liquids and
dissolved solids
2-Deformulation of products
3-Quantification of mixture components

11. NMR analysis capabilities :
1- Quantitative NMR Analysis (qNMR)
2-solid State Nuclear Magnetic Resonance
3-Solution State NMR
4-NMR Chemical Composition
5-Elucidation

12. .NMR analysis expertise
laboratories provide clients analytical
data regarding the type, quantity and
arrangement of atoms in chemical
systems, liquids and solids.

13. 1-Quantitative NMR Analysis :-
Intertie NMR laboratories
provide qNMR (Quantitative
Nuclear Magnetic Resonance
Spectroscopy) analysis
applications such as quality
control, drug impurity
analysis, and metabonomics
in agrochemicals, chemicals,
plastics processing and the
pharmaceutical industry

14. 2-Solid State Nuclear Magnetic Resonance
Solid state Nuclear Magnetic
Resonance (NMR) is a
specialized characterization
technique used to determine
chemical composition or
structural properties in a
variety of industrially relevant
materials. Solid State NMR
brings the benefits of solutions
NMR (detailed chemical
structural information without
the need for standards

15. Determination of catalyst
Heterogeneous catalysts used for
chemical manufacture, petroleum
processes, purification, and air
separation
Determine framework
composition of zeolites and changes
with activation or use
Identify adsorbed species in
porous materials
Characterize key functionalities in
insoluble residues

16. • Correlation spectroscopy:-
•
is one of several types of two-dimensional
nuclear magnetic resonance (NMR)
spectroscopy .This type of NMR experiment is
best known by its acronym .
• Biomolecular NMR spectroscopy:-
• A-proteins:-Much of the innovation within
NMR spectroscopy has been within the field
of protein NMR spectroscopy, an important
technique in structural biology .

17. • B-Nuculeic acids :-
• is the use of NMR spectroscopy to obtain
information about the structure and
dynamics of polynuculeic acid, such
as DNA or RNA.
• Note :-Nucleic acid and protein NMR
spectroscopy are similar but differences exist.
Nucleic acids have a smaller percentage of
hydrogen atoms, which are the atoms usually
observed in NMR spectroscopy.

18. NMR History
1937 Rabi predicts and observes nuclear magnetic
resonance
1946 Bloch, Purcell first nuclear magnetic resonance of
bulk sample
1966 Ernst, Anderson Fourier transform NMR
1944 Physics Rabi (Columbia)
1952 Physics Bloch (Stanford), Purcell (Harvard)
1991 Chemistry Ernst (ETH)
2003 Medicine Lauterbur (University of Illinois in Urbana)

19. Some NMR Web Sites
The Basics of NMR by J.P. Hornak Hypertext based NMR course
http://www.cis.rit.edu/htbooks/nmr/nmr-main.htm
Integrated Spectral Data Base System for Organic Compounds
http://www.aist.go.jp/RIODB/SDBS/menu-e.html
Educational NMR Software All kinds of NMR software
http://www.york.ac.uk/depts/chem/services/nmr/edusoft.html
NMR Knowledge Base A lot of useful NMR links
http://www.spectroscopynow.com/
NMR Information Server News, Links, Conferences, Jobs
http://www.spincore.com/nmrinfo/
Technical Tidbits Useful source for the art of shimming
http://www.acornnmr.com/nmr_topics.htm
BMRB (BioMagResBank) Database of NMR resonance assignments
http://www.bmrb.wisc.edu/

22. To
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