Gibson Assembly: a seamless molecular cloning techniqueyedeetay
This is an Open Educational Resource (OER) coursework for my Digital Education module. This OER compares and contrasts conventional restriction-based cloning with modern Gibson Assembly. The graphics in the Gibson Assembly sections can be easily reused and customized with freeware such as Inkscape to construct high-resolution PowerPoint animation in Molecular Cloning lecture.
Gibson Assembly: a seamless molecular cloning techniqueyedeetay
This is an Open Educational Resource (OER) coursework for my Digital Education module. This OER compares and contrasts conventional restriction-based cloning with modern Gibson Assembly. The graphics in the Gibson Assembly sections can be easily reused and customized with freeware such as Inkscape to construct high-resolution PowerPoint animation in Molecular Cloning lecture.
Sequencing genes and genomes in biology. The most important technique available to the molecular biologist is DNA sequencing, by which the precise order of nucleotides in a piece of DNA can be determined
Casting a Wider Net in Zebrafish Screening with Automated Microscopy and Imag...InsideScientific
Zebrafish are rapidly becoming a popular model organism for in vivo studies, particularly for drug screening and toxicology studies. Their benefits include fast development, economical husbandry, and direct amenability to microscopy since embryos are transparent. While imaging is fairly straightforward, in many cases, a substantial bottleneck to automated workflows is image analysis.
In this webinar, Dr. Jason Otterstrom and Dr. Alexandra Lubin describe an AI-powered analysis platform developed to enable true high-content screening of zebrafish, and highlight a range of applications where they have validated its performance. In brief, the easy-to-use software automatically identifies the fish outline, and internal anatomy & body regions with no required user inputs. They demonstrate the platform’s applicability in the context of counting GFP-labeled hematopoietic stem cells specifically in the tail region, along with measurement of x-ray induced apoptosis and dual-color analysis.
Key Topics Include:
- What is high-content imaging and how does it apply to Zebrafish
- How Deep-Learning can make analysis of zebrafish images truly high-content by extracting the fish’s anatomy
- Learn example assays where automated microscopy can facilitate use of zebrafish for screening studies
- One solution to orient zebrafish embryos without manual manipulation through specialized plates and software
Sequencing genes and genomes in biology. The most important technique available to the molecular biologist is DNA sequencing, by which the precise order of nucleotides in a piece of DNA can be determined
Casting a Wider Net in Zebrafish Screening with Automated Microscopy and Imag...InsideScientific
Zebrafish are rapidly becoming a popular model organism for in vivo studies, particularly for drug screening and toxicology studies. Their benefits include fast development, economical husbandry, and direct amenability to microscopy since embryos are transparent. While imaging is fairly straightforward, in many cases, a substantial bottleneck to automated workflows is image analysis.
In this webinar, Dr. Jason Otterstrom and Dr. Alexandra Lubin describe an AI-powered analysis platform developed to enable true high-content screening of zebrafish, and highlight a range of applications where they have validated its performance. In brief, the easy-to-use software automatically identifies the fish outline, and internal anatomy & body regions with no required user inputs. They demonstrate the platform’s applicability in the context of counting GFP-labeled hematopoietic stem cells specifically in the tail region, along with measurement of x-ray induced apoptosis and dual-color analysis.
Key Topics Include:
- What is high-content imaging and how does it apply to Zebrafish
- How Deep-Learning can make analysis of zebrafish images truly high-content by extracting the fish’s anatomy
- Learn example assays where automated microscopy can facilitate use of zebrafish for screening studies
- One solution to orient zebrafish embryos without manual manipulation through specialized plates and software
The Genome editing Era (CRISPER Cas 9) : State of the Art and Perspectives fo...Anand Choudhary
Role of CRISPR/Cas9 in plant pathology
Production of disease resistance cultivars by editing the genome which is responsible for susceptibility factor for fungal and bacterial diseases.
By editing the genome which governs host pathogen interaction we can obtain incompatible interaction between host pathogen.
To improve the efficacy of bio control agents.
By editing the genome responsible for virus multiplication and virulence we can obtain virus free resistance cultivars.
The Genome-editing Era (CRISPER Cas 9) : State of the Art and Perspectives fo...ANAND CHOUDHARY
Role of CRISPR/Cas9 in plant pathology
Production of disease resistance cultivars by editing the genome which is responsible for susceptibility factor for fungal and bacterial diseases.
By editing the genome which governs host pathogen interaction we can obtain incompatible interaction between host pathogen.
To improve the efficacy of bio control agents.
By editing the genome responsible for virus multiplication and virulence we can obtain virus free resistance cultivars.
An Introduction to Crispr Genome Editing
Crispr cas: A new tool of genome editing
CRISPRs (Clustered Regularly Interspaced Short Palindromic Repeats) are part of an adaptive defense mechanism in bacteria and archaea. Use of the CRISPR/Cas9 system for genome editing has been a major technological breakthrough, making genome modification in cells or organisms fast, more efficient, and much more robust than previous genome editing methods. Single guide RNAs (sgRNAs) or guide RNAs (gRNAs) direct and activate the Cas9 endonuclease at a specific genomic sequence. Cas9 then cleaves the target DNA, making it available for repair by the non-homologous end joining (NHEJ) system or for creating an insertion site for exogenous donor DNA by homologous recombination.
CRISPR (clustered regularly interspaced short palindromic repeats) is a family of DNA sequences found within the genomes of prokaryotic organisms such as bacteria and archaea. These sequences are derived from DNA fragments of bacteriophages that have previously infected the prokaryote and are used to detect and destroy DNA from similar phages during subsequent infections. Hence these sequences play a key role in the antiviral defense system of prokaryotes.
Cas9 (CRISPR-associated protein 9) is an enzyme that uses CRISPR sequences as a guide to recognize and cleave specific strands of DNA that are complementary to the CRISPR sequence. Cas9 enzymes together with CRISPR sequences form the basis of a technology known as CRISPR-Cas9 that can be used to edit genes within organisms.This editing process has a wide variety of applications including basic biological research, development of biotechnology products, and treatment of diseases.
The CRISPR-Cas system is a prokaryotic immune system that confers resistance to foreign genetic elements such as those present within plasmids and phages that provides a form of acquired immunity. RNA harboring the spacer sequence helps Cas (CRISPR-associated) proteins recognize and cut foreign pathogenic DNA. Other RNA-guided Cas proteins cut foreign RNA. CRISPR are found in approximately 50% of sequenced bacterial genomes and nearly 90% of sequenced archaea.
The CRISPR (clustered regularly interspaced short palindromic repeats)–Cas9 (CRISPR-associated nuclease 9), a genome editing system adapted from the bacterial immune mechanism that is poised to transform genetic engineering by providing a simple, efficient and economical method to precisely manipulate the genome of any organism. Compared with zinc finger nucleases (ZFN) and transcription activator-like effector nucleases (TALEN), CRISPR/Cas9 is simpler with higher specificity and less toxicity. This RNA-guided nuclease (RGN)-based approach has been effectively used to induce targeted mutations(knock in or knock out) in multiple genes simultaneously, create conditional alleles, and generate endogenously tagged proteins.It has a wide variety of applications such as gene therapy, gene expression regulation, genome wide functional screening, virus resistance, transgenic animal production, site specific DNA integration etc. In the future CRISPR/Cas9 technology will play a significant role in innovating the life science research and industrial fields.
Biological screening of herbal drugs: Introduction and Need for
Phyto-Pharmacological Screening, New Strategies for evaluating
Natural Products, In vitro evaluation techniques for Antioxidants, Antimicrobial and Anticancer drugs. In vivo evaluation techniques
for Anti-inflammatory, Antiulcer, Anticancer, Wound healing, Antidiabetic, Hepatoprotective, Cardio protective, Diuretics and
Antifertility, Toxicity studies as per OECD guidelines
How to Make a Field invisible in Odoo 17Celine George
It is possible to hide or invisible some fields in odoo. Commonly using “invisible” attribute in the field definition to invisible the fields. This slide will show how to make a field invisible in odoo 17.
The Roman Empire A Historical Colossus.pdfkaushalkr1407
The Roman Empire, a vast and enduring power, stands as one of history's most remarkable civilizations, leaving an indelible imprint on the world. It emerged from the Roman Republic, transitioning into an imperial powerhouse under the leadership of Augustus Caesar in 27 BCE. This transformation marked the beginning of an era defined by unprecedented territorial expansion, architectural marvels, and profound cultural influence.
The empire's roots lie in the city of Rome, founded, according to legend, by Romulus in 753 BCE. Over centuries, Rome evolved from a small settlement to a formidable republic, characterized by a complex political system with elected officials and checks on power. However, internal strife, class conflicts, and military ambitions paved the way for the end of the Republic. Julius Caesar’s dictatorship and subsequent assassination in 44 BCE created a power vacuum, leading to a civil war. Octavian, later Augustus, emerged victorious, heralding the Roman Empire’s birth.
Under Augustus, the empire experienced the Pax Romana, a 200-year period of relative peace and stability. Augustus reformed the military, established efficient administrative systems, and initiated grand construction projects. The empire's borders expanded, encompassing territories from Britain to Egypt and from Spain to the Euphrates. Roman legions, renowned for their discipline and engineering prowess, secured and maintained these vast territories, building roads, fortifications, and cities that facilitated control and integration.
The Roman Empire’s society was hierarchical, with a rigid class system. At the top were the patricians, wealthy elites who held significant political power. Below them were the plebeians, free citizens with limited political influence, and the vast numbers of slaves who formed the backbone of the economy. The family unit was central, governed by the paterfamilias, the male head who held absolute authority.
Culturally, the Romans were eclectic, absorbing and adapting elements from the civilizations they encountered, particularly the Greeks. Roman art, literature, and philosophy reflected this synthesis, creating a rich cultural tapestry. Latin, the Roman language, became the lingua franca of the Western world, influencing numerous modern languages.
Roman architecture and engineering achievements were monumental. They perfected the arch, vault, and dome, constructing enduring structures like the Colosseum, Pantheon, and aqueducts. These engineering marvels not only showcased Roman ingenuity but also served practical purposes, from public entertainment to water supply.
2024.06.01 Introducing a competency framework for languag learning materials ...Sandy Millin
http://sandymillin.wordpress.com/iateflwebinar2024
Published classroom materials form the basis of syllabuses, drive teacher professional development, and have a potentially huge influence on learners, teachers and education systems. All teachers also create their own materials, whether a few sentences on a blackboard, a highly-structured fully-realised online course, or anything in between. Despite this, the knowledge and skills needed to create effective language learning materials are rarely part of teacher training, and are mostly learnt by trial and error.
Knowledge and skills frameworks, generally called competency frameworks, for ELT teachers, trainers and managers have existed for a few years now. However, until I created one for my MA dissertation, there wasn’t one drawing together what we need to know and do to be able to effectively produce language learning materials.
This webinar will introduce you to my framework, highlighting the key competencies I identified from my research. It will also show how anybody involved in language teaching (any language, not just English!), teacher training, managing schools or developing language learning materials can benefit from using the framework.
A Strategic Approach: GenAI in EducationPeter Windle
Artificial Intelligence (AI) technologies such as Generative AI, Image Generators and Large Language Models have had a dramatic impact on teaching, learning and assessment over the past 18 months. The most immediate threat AI posed was to Academic Integrity with Higher Education Institutes (HEIs) focusing their efforts on combating the use of GenAI in assessment. Guidelines were developed for staff and students, policies put in place too. Innovative educators have forged paths in the use of Generative AI for teaching, learning and assessments leading to pockets of transformation springing up across HEIs, often with little or no top-down guidance, support or direction.
This Gasta posits a strategic approach to integrating AI into HEIs to prepare staff, students and the curriculum for an evolving world and workplace. We will highlight the advantages of working with these technologies beyond the realm of teaching, learning and assessment by considering prompt engineering skills, industry impact, curriculum changes, and the need for staff upskilling. In contrast, not engaging strategically with Generative AI poses risks, including falling behind peers, missed opportunities and failing to ensure our graduates remain employable. The rapid evolution of AI technologies necessitates a proactive and strategic approach if we are to remain relevant.
Introduction to AI for Nonprofits with Tapp NetworkTechSoup
Dive into the world of AI! Experts Jon Hill and Tareq Monaur will guide you through AI's role in enhancing nonprofit websites and basic marketing strategies, making it easy to understand and apply.
Macroeconomics- Movie Location
This will be used as part of your Personal Professional Portfolio once graded.
Objective:
Prepare a presentation or a paper using research, basic comparative analysis, data organization and application of economic information. You will make an informed assessment of an economic climate outside of the United States to accomplish an entertainment industry objective.
Welcome to TechSoup New Member Orientation and Q&A (May 2024).pdfTechSoup
In this webinar you will learn how your organization can access TechSoup's wide variety of product discount and donation programs. From hardware to software, we'll give you a tour of the tools available to help your nonprofit with productivity, collaboration, financial management, donor tracking, security, and more.
Honest Reviews of Tim Han LMA Course Program.pptxtimhan337
Personal development courses are widely available today, with each one promising life-changing outcomes. Tim Han’s Life Mastery Achievers (LMA) Course has drawn a lot of interest. In addition to offering my frank assessment of Success Insider’s LMA Course, this piece examines the course’s effects via a variety of Tim Han LMA course reviews and Success Insider comments.
Acetabularia Information For Class 9 .docxvaibhavrinwa19
Acetabularia acetabulum is a single-celled green alga that in its vegetative state is morphologically differentiated into a basal rhizoid and an axially elongated stalk, which bears whorls of branching hairs. The single diploid nucleus resides in the rhizoid.
Advance in Industrial Biotechnology using CRISPER Cas9 System| Presentation | Shahraiz
1. Advance in Industrial Biotechnology Using
CRISPER Cas9 System
Group Members
Shahraiz Butt (Leader)(s2018231023)
M.Sufyan Nazeer(s2018231027)
Shagufta Arshad(s2018231061)
Rimsha Waheed(s2018231054)
Rahat Roshni(s2018231065)
School of Science, University of Management and Technology
2. Table of Contents
➢Introduction
➢CRISPR Locus
➢Components of CRISPR Cas9 System
➢About Cas9
➢Cas9 Lobes
➢Guide RNA
➢Mechanism
➢Summary
➢Prons and Cons
➢Conclusion
3. Introduction
➢CRISPR stands for clustered regularly interspaced short
palindromic repeats.
➢Genome editing technique
➢Remove, add and alter genome
➢Immune system in Bacteria and Archaea
➢Anti virus system
➢CRISPR locus is Vaccinated Card
4. CRISPR Locus
➢CRISPR locus consists of:
➢CRISPR Array, a short
sequence of nucleotide about
25-40
➢separated by short spacer
➢Cas gene encode protein like
endonucleases etc.
5. Components of CRISPR -Cas System
➢Spacer separates nucleotide sequence, drive from invading
viruses
➢Leader conserved sequence with CRISPR present upstream
➢Protospacer targeted sequence
➢PAM(protospacer associated motif) present next to target
sequence mecessary for recognition
➢crRNA is CRISPR RNA
6. About Cas9
➢Cas9 is RNA guided DNA endonuclease
➢Targets specific DNA sequence
➢crRNA require for Cas9 activation
Types of Cas9 are:
➢ Spy Cas9 (streptococcus pyrogene)
➢ StCas9 (streptococcus thermophilus)
➢ TdCas9 (Treponema denticola)
7. Cas9 Lobes
Cas9 protein has two lobes:
➢Recognition lobe
identification through PAM
➢Nuclease lobe- cleavage ,has
two domains:
➢HNH domain cleaves
complementary strand
➢RuvC domain cleaves non-
complementary strand
8. Guide RNA
➢Engineered 5’ end that is
complementary to target
DNA sequence.
➢Binds with Cas9, induces:
• Conformatinal changes
• Make it active
9. Mechanism
➢Cas9 searches for target sequence through PAM
➢Guide RNA has 5’ end complementary to target, helps in
binding
➢After binding HNH and RuvC will cut target DNA
➢DSB (double stranded break) will occur
10.
11. Repairing of DSB
➢Blunt ends are repared by cell’s natural environment
Two mechanisms are followed:
➢NHEJ break ends directly ligated without the need of
homologous template
➢HDR require homologous template to guide repair
14. Pros and Cons
• PROS
➢Fast than others
➢Use in many species
➢Abilty to target any genomic
region
• CONS
➢off target effect
➢Ethical
➢Social effect in society
15. Conclusion
➢CRISPR has both advantages and disadvantages ranging
from ethical concern. BUT...!
This scientific breakthrough has the ability to:
➢Eliminate disease
➢To solve world hunger
➢Authorities sould formulate laws for its safe and ethical use
