Objectives :- * Characterization and study of biochar.     *To examine biochar adsorption capacity.   *To determine the effect of KMnO4 as modifying agent on biochar adsorption.

1. “Adsorption of Mercury metal using modified
vermicompost biochar ”
Presented By :
Ravindra Kumar Kachhap Oraon
B.Sc.(Hons.) Biotechnology , 3rd Year
Department Of Biotechnology
Roll No : 180500028
SALS, Uttaranchal University
Batch :- 2018-21
Work done
under the supervision of
Dr. Indra Rautela
Assistant Professor

2. TABLE OF CONTENTS
Aim and Objectives
Introduction
Material and Methods
Result Discussion
Conclusion
References

3. Aim : Adsorption of Mercury metal using modified vermicompost biochar.
Objectives :
 Characterization and study of biochar.
 To examine biochar adsorption capacity.
 To determine the effect of KMnO4 as modifying agent on biochar adsorption.
 The advancement of technology in agricultural and industrial areas progresses a lot. But it also leaves a footprint of many harmful chemicals
and heavy metals extracted from these sectors pollutes the environment, affects biodiversity which leads to many carcinogenic and other
diseases such as Cancer, stomach-ulcer, diabetes, asthma, liver damage, renal failure, neurological disorders in human beings (Elghany et al.,
1990; Kazantzis, 1979; Volesky and Holan, 1995; Sud et al., 2008; Zhang et al., 2019; Nordberg et al., 2018).
 In India mainly the farmers are dependent on chemical pesticides to increase the growth of plants and the industries that pass out their
wastewater in the water sources disturbs the water eco-system. So, it’s very necessary to adopt eco-friendly systems to filter these chemicals
effectively.
INTRODUCTION

4.  Utilizing the agricultural wastes as vermicompost a unique way to resolve all these problems that occurred by agricultural
wastes. By microorganisms and earthworms (Eisenia fetida); the biowastes are converted into vermicompost through a
decomposition process called vermicomposting.
 These vermicomposts enhance the adsorption of heavy metals effectively and used as a biochar.
 Biochar formed at high temperatures using a muffle furnace through pyrolysis.
 To make biochar effective it should be treated with 7.5% KMnO4 solution for 6 hrs at 25̊C due to its high specific area.
Adsorption capacity increases by this treatment process and helps to remove heavy metals more effectively.
 By analyzing the existing circumstances of contamination in the environment this research has been designed accordingly to
get rid out of these pollutants through cost effective technology.

5. MATERIALS & METHODS
Prepration of Vermicompost
 An organic product prepared with the help of an earthworm called Estenia foteda or red wrigglers.
 Organic wastes are collected from agriculture sectors and prepared a bed for vermicomposting using red wrigglers, cow
dung, and soil.
 The organic wastes are fully decomposed and enriched with useful micro-nutrients which are eco-friendly to the
environment.
 Takes a month to convert organic wastes into compost.
Formulation of Biochar
 Prepared vermicompost is converted into biochar by the process of pyrolysis.
 To convert vermicompost into biochar it should be converted into a flyash firstly using microwave till the vermicompost
converted into flyash then after it should be kept in a muffle furnace at 500̊C for 2-3hrs to get biochar.
 The biochar is treated with KMnO4 for 6hrs at 25̊C to make biochar more effective so that the adsorption capacity
increases and adsorbs the heavy metals more from the polluted water.

6. VERMICOMPOST
FLYASH BIOCHAR
Formation of different stocks and experiment reagents.
 Stock solution of Mercury Chloride of different concentrations such as 15mg/l, 30mg/l, 60mg/l, 90mg/l respectively is
prepared at different pH, concentrations of stock solutions obtained by adding 1000ml of distilled water to maintain the
concentrations.
 All these experiments were carried out in 1000ml of beaker in the laboratory at room temperature.
Analysis of heavy metal absorbance by biochar
Metal absorbance of filtrate samples are determined through spectrophotometer at 520nm.

7. Result discussion
 Different experimental sets are desingn to investigated the absorbance pattern of 15mg, 30mg, 60mg and 90mg
concentration of HgCl2 over the fixed amount (0.5g) of activated biochar together with numerous pH range (2,4,6 and 8)
and contact time period (30,60,90 and 120 min) . All the combination are tested for the absorption behavior for mercury
are shown in (Graph 1 to graph 4).
 The coming results for metal entrapment shows that 30, 60 and 90mg concentrations are best absorbed at pH 6/ 60 min
time period combination.
 While high concentration of Hg 90mg shows maximum absorbance at pH 6/90 min. Beside these physical range metal
absorption is quit abbreviated.
Graph 1-for 15mg HgCl2
Graph 2-for 30mg HgCl2

8. Graph 3-for 30mg HgCl2 Graph 4-for 90mg HgCl2
Fig 1-for 15mg HgCl2 ,
pH 2
Fig 2-for 15mg HgCl2 ,
pH 4
Fig 3-for 15mg HgCl2 ,
pH 6

9. Fig 4-for 15mg
HgCl2 , pH 8
Fig 5-for 30mg
HgCl2 , pH 2
Fig 6-for 30mg
HgCl2 , pH 4
Fig 7-for 30mg HgCl2
, pH 6
Fig 8-for 30mg
HgCl2 , pH 8
Fig 9-for 60mg HgCl2
, pH 2

10. Fig 10-for 60mg
HgCl2 ,pH 4
Fig 11-for 60mg
HgCl2 ,pH 6
Fig 12-for 60mg
HgCl2 ,pH 8
Fig 13-for 90mg
HgCl2 , pH 2- pH4
Fig 14-for 90mg
HgCl2 , pH 4 – pH 6
Fig 15-for 90mg
HgCl2 ,pH 8

11. Conclusion
 Bioabsorption of different heavy metal through clost effect vermicopost biochar smethod have seen to be
promaisng method to replaced other previously existing waste management system.
 It can concluded that activated biochar showed high rate of adsorption for mercury compare to non-
treated vermicompost. Utilization od adavnse tools like FTIR,XRD and GC-MS analysis futher confirmed
the texture and chemical behavior of biochar for its advancement.

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14. THANK YOU