The document describes the OECD guideline for conducting an acute oral toxicity test using the up-down procedure to estimate the LD50 of a test substance in rats. It involves an initial limit test, followed by a main test if needed. In the limit test, animals are dosed sequentially at a limit dose of 2000 or 5000 mg/kg, with testing stopping depending on survival outcomes. The main test uses a single animal dosed at a time based on the response of previous animals, with dosing stopped based on predefined criteria. Animals are observed for 14 days and necropsied, with individual animal data, summaries, and test results reported. The goal is to minimize animal use while obtaining an estimate of the substances acute oral