Microbes were isolated from two types of soil, normal soil and chicken farm soil, using a spread-plate method to explore the different microbes inhabiting each soil. More bacterial colonies formed from chicken farm soil compared to normal soil, and more diverse colony morphologies were observed from chicken farm soil. Bacterial growth was favored on nutrient agar containing 7.5% NaCl. Four samples with distinct colony morphologies were selected for staining to identify the bacteria, which were found to be gram-negative bacillus bacteria and one colony with vegetative cells containing endospores.

1. ABSTRACT
Microbes are able to adapt itself to survive in the harsh environment, they thrive
everywhere. However, soil is a medium that rich of various types of microbes. Thus in
order to explore the types of microbes that are habituating in soil, an experiment of
isolate microbes from soil is carried out with using spread-plate method. There are two
types of soil is isolated separately which are normal soil and chicken farm soil to
discovered the different of microbes that found in different types of soil. Typically, a
small amount of 0.1mL to 1 mL is taken to be further diluted, then is spread onto the
agar. There are two different agar is prepared that are nutrient agar and nutrient agar
that contains 7.5% of NaCl to provide two different environments for the microbes. In
this experiment, Standard Plate Count or called Viable Plate Count is used to determine
the number of colonies of bacterial cells in the agar plate with counting by colony-
forming units (CFUs). After a week, the number of colony of bacteria from dilution of
chicken farm soil is much higher compared to normal soil. Whereas there are more
different types of morphology of colonies that are been seen from the plate of agar
that spread from the dilution of chicken farm soil than normal soil too. From the result
of CFUs, the growth of bacterial is more favour in nutrient agar with 7.5% NaCl than
normal media. After that, there are four samples with different significant in colony
morphology is then selected to differentiated the types of bacteria by Simple Gram
staining and Endospore staining procedure. The bacteria from the soil are gram-
negative bacillus bacteria as its cells are showed in rod-shaped pink and red colour.
For the endospore staining, there are only one colony microbes are vegetative cell with
endospores as the spores still retain green and the vegetative cell is stained with pink
colour. In short, the types of microbes is able to determine out and the result of this
experiment is under expected.