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A mistake occurs during lambda phage excision from the E. coli genome: a portion of the E.
colichromosome is excised with the phage genome, leaving behind a portion of the lambda
genome that includes the int gene. This defective genome is packaged and infects an E. coli
strain that is recA- (it lacks RecA). What do you think happens to this genetic material?
1) injected DNA is degraded
2) integrates into new host genome
3) The transferred portion of E. coli chromosome recombines with the new host genome
4) All of these are possible
Solution
The answer is Option no 1 that is injected DNA is degraded.
The defective genome does not carry int gene. So, the lambda phge cannot insert its genetic
material into host genome.
Homologus recombination and DNA reapir does not possible because the host genome is recA-
Strain.(rec A protein plays a major role in homologus recombination).

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  • 1. A mistake occurs during lambda phage excision from the E. coli genome: a portion of the E. colichromosome is excised with the phage genome, leaving behind a portion of the lambda genome that includes the int gene. This defective genome is packaged and infects an E. coli strain that is recA- (it lacks RecA). What do you think happens to this genetic material? 1) injected DNA is degraded 2) integrates into new host genome 3) The transferred portion of E. coli chromosome recombines with the new host genome 4) All of these are possible Solution The answer is Option no 1 that is injected DNA is degraded. The defective genome does not carry int gene. So, the lambda phge cannot insert its genetic material into host genome. Homologus recombination and DNA reapir does not possible because the host genome is recA- Strain.(rec A protein plays a major role in homologus recombination).