Central Dogma Assignment Print Name ___________________________________________ Directions: Complete this worksheet after listening to the Central Dogma recorded lecture posted on Blackboard. Each student will turn in his/her individual worksheet at the beginning of class. COMPLETE THE TABLE. LABEL YOUR 5’ AND 3’ ENDS. YOU MUST GET THE ENTIRE TABLE CORRECT FOR CREDIT. NO PARTIAL CREDIT. DNA template (-) 5’ GTACTATTAATT TACTGGGTGCAT CCGCATGGTACG 3’ DNA coding (+) strand mRNA (+) codons Only write the tRNA anticodons for actual codons coding for amino acids amino acid sequence 1. You isolate and sequence a novel cytoplasmic membrane protein from Staphylococcus aureus that appears to function in the transport of cations into the cell. A colleague working in a competing lab in France isolates a cytoplasmic membrane protein from Streptococcus pyogenes that appears to have the identical function as your protein. In fact, the primary structure of the S. pyogenes protein is identical to the S. aureus protein. Does this mean that the genes for these two proteins are identical in Staphylococcus aureus and Streptococcus pyogenes? Explain your answer. 2. What is the effect of DNA mutation on protein sequence? 3. In sickle-cell disease, the replacement of A by T at the 17th nucleotide of the gene for the beta chain of hemoglobin changes the mRNA codon as shown below (in red). Wild-type mRNA 5’… A C U C C U G A G G A G… Sickle-cell mRNA 5’… A C U C C U G U G G A G…. a. What amino acids are encoded by the two mRNAs? Wild-type amino acids: Sickle-cell amino acids: b. What kind of mutation does this cause in the protein? Bio 241 Quiz #3 March 22, 2018 Print First and Last Name _______________________________________ I. Multiple Choice: Read each question carefully and select the one best answer for each question. Write your answer using CAPITAL LETTERS on the line in front of each question. Questions will be counted wrong if the answer is not written on the line. (1 point each) _____1. Which of the following is incorrect? A. RNA polymerase – makes a molecule of RNA from an RNA template B. DNA polymerase – makes a molecule of DNA from a DNA template C. DNA ligase – joins segments of DNA D. DNA gyrase (topoisomerase) – releases overwinding strain and untwists DNA E. All of the above are correct. _____2. A nucleotide is A. a five-carbon sugar, a phosphate group and a nitrogen-containing base B. a sugar with an attached base containing nitrogen C. a six-carbon sugar, a phosphate group and a nitrogen-containing base D. a nitrogen-containing base with an attached five-carbon sugar E. A is correct for DNA and C is correct for RNA _____3. In DNA synthesis, the primer is synthesized by A. RNA polymerase B. DNA polymerase I C. DNA polymerase III D. Helicase E. DNA gyrase ...