Gene therapy is an experimental technique that uses genes to treat or prevent disease which allow doctors to treat a disorder by inserting a gene into a patientâs cells instead of using drugs or surgery.
Gene therapy is an experimental technique that uses genes to treat or prevent disease which allow doctors to treat a disorder by inserting a gene into a patientâs cells instead of using drugs or surgery.
BIO 106
Lecture 13: Genetic Engineering and Biotechnology
A. Recombinant DNA/ Genetic Engineering
B. Applications of Genetic Engineering
1. Researches on Human Genes
2. Researches on Animal Genes
3. Researches on Plant Genes
4. Researches on Microbial Genes
C. The Release of Genetically Engineered Organisms
1. Biosafety and Ecological Implications
1.1 Potential Ecological Concerns
1.2 Regulatory Policies
The use of genetic engineering technology in animals has been associated with ethical issues, some of which relate to animal welfare. Discuss examples of genetically engineered animals and evaluate the ethical concerns of genetic engineering.
BIO 106
Lecture 13: Genetic Engineering and Biotechnology
A. Recombinant DNA/ Genetic Engineering
B. Applications of Genetic Engineering
1. Researches on Human Genes
2. Researches on Animal Genes
3. Researches on Plant Genes
4. Researches on Microbial Genes
C. The Release of Genetically Engineered Organisms
1. Biosafety and Ecological Implications
1.1 Potential Ecological Concerns
1.2 Regulatory Policies
The use of genetic engineering technology in animals has been associated with ethical issues, some of which relate to animal welfare. Discuss examples of genetically engineered animals and evaluate the ethical concerns of genetic engineering.
Gene transfer technology pharmacology biotechnology basic methods
Natural, physical, chemical methods of gene transfer.
Along with advantages and limitations, and applications.
TRANSGENIC CROPS CHALLENGES AND PROSPECTS
Transgenic Technology : Transform gene from any source.
Eg: animals, bacteria, virus etc
Traditional Breeding : Move genes only between members of a particular genus of plants.
Take multiple growing seasons to develop and test a new variety.
Lot of man power
Limited possibility of improved traits.
This presentation aims to provide an in-depth understanding of the science behind creating transgenic animals, explore their potential applications, and delve into the ethical considerations surrounding this emerging field of research.
Definition and Background:
We begin by defining transgenic animals as organisms that have had their genetic material intentionally altered through the introduction of foreign genes. This groundbreaking field of genetic engineering has its roots in the development of recombinant DNA technology in the 1970s, which enabled the transfer of genes across different species.
Genetic Engineering Techniques:
This section delves into the techniques employed to create transgenic animals, emphasizing the following key methodologies:
a. DNA Microinjection: The introduction of foreign DNA into the pronucleus of a fertilized embryo, allowing the foreign gene to be incorporated into the animal's genome and expressed in its cells.
b. Gene Targeting: The precise modification of an organism's genome by replacing or disrupting specific genes using technologies such as homologous recombination or CRISPR-Cas9.
c. Somatic Cell Nuclear Transfer (SCNT): The cloning technique involving the transfer of a nucleus from a somatic cell into an enucleated egg, resulting in the creation of an embryo with the same genetic makeup as the somatic cell donor.
Applications of Transgenic Animals:
This section explores the wide-ranging applications of transgenic animals across various fields, including:
a. Biomedical Research: Transgenic animals serve as invaluable models for studying human diseases and testing potential therapies, enabling significant advancements in medical research.
b. Agriculture: Transgenic animals can be engineered to possess desirable traits, such as increased resistance to diseases or improved meat quality, offering the potential to enhance agricultural productivity and sustainability.
c. Pharmaceutical Production: Transgenic animals can be designed to produce therapeutic proteins or antibodies in their milk or blood, providing a cost-effective means of manufacturing valuable pharmaceutical products.
d. Organ Transplantation: Research on transgenic animals has explored the possibility of generating organs that are genetically compatible with humans, addressing the shortage of donor organs for transplantation.
Epistemic Interaction - tuning interfaces to provide information for AI supportAlan Dix
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Paper presented at SYNERGY workshop at AVI 2024, Genoa, Italy. 3rd June 2024
https://alandix.com/academic/papers/synergy2024-epistemic/
As machine learning integrates deeper into human-computer interactions, the concept of epistemic interaction emerges, aiming to refine these interactions to enhance system adaptability. This approach encourages minor, intentional adjustments in user behaviour to enrich the data available for system learning. This paper introduces epistemic interaction within the context of human-system communication, illustrating how deliberate interaction design can improve system understanding and adaptation. Through concrete examples, we demonstrate the potential of epistemic interaction to significantly advance human-computer interaction by leveraging intuitive human communication strategies to inform system design and functionality, offering a novel pathway for enriching user-system engagements.
Kubernetes & AI - Beauty and the Beast !?! @KCD Istanbul 2024Tobias Schneck
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As AI technology is pushing into IT I was wondering myself, as an âinfrastructure container kubernetes guyâ, how get this fancy AI technology get managed from an infrastructure operational view? Is it possible to apply our lovely cloud native principals as well? What benefitâs both technologies could bring to each other?
Let me take this questions and provide you a short journey through existing deployment models and use cases for AI software. On practical examples, we discuss what cloud/on-premise strategy we may need for applying it to our own infrastructure to get it to work from an enterprise perspective. I want to give an overview about infrastructure requirements and technologies, what could be beneficial or limiting your AI use cases in an enterprise environment. An interactive Demo will give you some insides, what approaches I got already working for real.
Connector Corner: Automate dynamic content and events by pushing a buttonDianaGray10
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Here is something new! In our next Connector Corner webinar, we will demonstrate how you can use a single workflow to:
Create a campaign using Mailchimp with merge tags/fields
Send an interactive Slack channel message (using buttons)
Have the message received by managers and peers along with a test email for review
But thereâs more:
In a second workflow supporting the same use case, youâll see:
Your campaign sent to target colleagues for approval
If the âApproveâ button is clicked, a Jira/Zendesk ticket is created for the marketing design team
Butâif the âRejectâ button is pushed, colleagues will be alerted via Slack message
Join us to learn more about this new, human-in-the-loop capability, brought to you by Integration Service connectors.
And...
Speakers:
Akshay Agnihotri, Product Manager
Charlie Greenberg, Host
Transcript: Selling digital books in 2024: Insights from industry leaders - T...BookNet Canada
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The publishing industry has been selling digital audiobooks and ebooks for over a decade and has found its groove. Whatâs changed? What has stayed the same? Where do we go from here? Join a group of leading sales peers from across the industry for a conversation about the lessons learned since the popularization of digital books, best practices, digital book supply chain management, and more.
Link to video recording: https://bnctechforum.ca/sessions/selling-digital-books-in-2024-insights-from-industry-leaders/
Presented by BookNet Canada on May 28, 2024, with support from the Department of Canadian Heritage.
LF Energy Webinar: Electrical Grid Modelling and Simulation Through PowSyBl -...DanBrown980551
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Do you want to learn how to model and simulate an electrical network from scratch in under an hour?
Then welcome to this PowSyBl workshop, hosted by Rte, the French Transmission System Operator (TSO)!
During the webinar, you will discover the PowSyBl ecosystem as well as handle and study an electrical network through an interactive Python notebook.
PowSyBl is an open source project hosted by LF Energy, which offers a comprehensive set of features for electrical grid modelling and simulation. Among other advanced features, PowSyBl provides:
- A fully editable and extendable library for grid component modelling;
- Visualization tools to display your network;
- Grid simulation tools, such as power flows, security analyses (with or without remedial actions) and sensitivity analyses;
The framework is mostly written in Java, with a Python binding so that Python developers can access PowSyBl functionalities as well.
What you will learn during the webinar:
- For beginners: discover PowSyBl's functionalities through a quick general presentation and the notebook, without needing any expert coding skills;
- For advanced developers: master the skills to efficiently apply PowSyBl functionalities to your real-world scenarios.
GraphRAG is All You need? LLM & Knowledge GraphGuy Korland
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Guy Korland, CEO and Co-founder of FalkorDB, will review two articles on the integration of language models with knowledge graphs.
1. Unifying Large Language Models and Knowledge Graphs: A Roadmap.
https://arxiv.org/abs/2306.08302
2. Microsoft Research's GraphRAG paper and a review paper on various uses of knowledge graphs:
https://www.microsoft.com/en-us/research/blog/graphrag-unlocking-llm-discovery-on-narrative-private-data/
Elevating Tactical DDD Patterns Through Object CalisthenicsDorra BARTAGUIZ
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After immersing yourself in the blue book and its red counterpart, attending DDD-focused conferences, and applying tactical patterns, you're left with a crucial question: How do I ensure my design is effective? Tactical patterns within Domain-Driven Design (DDD) serve as guiding principles for creating clear and manageable domain models. However, achieving success with these patterns requires additional guidance. Interestingly, we've observed that a set of constraints initially designed for training purposes remarkably aligns with effective pattern implementation, offering a more âmechanicalâ approach. Let's explore together how Object Calisthenics can elevate the design of your tactical DDD patterns, offering concrete help for those venturing into DDD for the first time!
Elevating Tactical DDD Patterns Through Object Calisthenics
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trnsgenic plants and animls
1.
2.
3. ACKNOWLEDGEMENT
âą I would like to express my special thanks of
gratitude to our Dean Ag. Professor.
SAMSHER SINGH, H.O.D. Professor. S.K.
BHATNAGAR sir, Dr. PURUSHOTTAM sir,
and Dr. ARCHANA BHATNAGAR mam ,
OIC OF BASIC SCIENCE AND
HUMANITY, who gave me the golden
opportunity to do this wonderful project on
the topic (TRANSGENIC PLANTS),
Secondly i would also like to thank my
instructor Ms. AASTHA MAM and Ms.
SWARNA MAM for their support and
6. Introduction
ï¶Transgenic plants are the results of modern
biotechnology.
ï¶An organism containing a transgene
introduced by technological (not breeding)
methods is called transgenic.
ï¶Transgenes are the genetically engineered
gene added to a species.
ï¶The process of producing transgenic
organism is called transgenesis.
8. Agrobacterium mediatedmethod
âą Agrobacterium-mediated transformation
is most commonly used method for
transgenesis.
âą A.tumefaciens is a rod shaped Gram
negative soil bacterium found in the
rhizosphere.
âą It contains Ti plasmid(tumor-inducing)
which causes crown gall disease in dicot
plants.
âą It mainly infect the wound site and
causes disease symptoms.
9. Gene gun method
ï¶The method was first used by Klein et
al.(1987) to transform epidermal cell of
Allium cepa.
ï¶This method is mainly used for cereals
transformation.
ï¶Good for plants that cannot be transformed
by Agrobacterium, e.g. most monocot
ï¶DNA is bound to tiny particles of gold or
tungsten which are subsequently shot into
plant tissue or single plant cells under high
pressure.The particles penetrate both
the cellwall and membranes.
10. Electroporation
âą In this technique , short
pulses of high voltage are
applied to protoplasts
which make temporary
pores in the plasma
membrane to increase
their permeability and
facilitate the uptake of
foreign gene.
11. Microinjection method
ï¶ Microinjection involves direct physical approach in
depositing DNA into specific target cells.
ï¶ The protoplasts are immobilized in agarose or on
glass slides coated with poly-lysine or by holding
them under suction by a micropipette.
ï¶ The exogenous DNA of interest is taken in
microinjector and then directly delivered inside the
cell.
ï¶ Micromanipulator is used for microinjection of the
DNA.
ï¶ A maximum of 40-50 protoplasts can be
microinjected in one hour.
ï¶ Successful transformation by microinjection of
cells has been achieved in tobacco,alfalfa etc.
12.
13. Applications
Insect resistant cotton â Bt toxin kills
the cotton boll worm
ï¶transgene = Bt gene from Bacillus
thuringensis
Insect resistant corn â Bt toxin kills
the European corn borer.
ï¶transgene = Bt protein
14. Herbicide resistant crops -
soybean, corn, canola
âąTransgene = EPSP synthase
Virus resistance papaya- resistant to
papaya ring spot virus
âąTransgene = virus coat protein
15. Transgenic plants in India
ï¶India cultivated its first transgenic Bt cotton crop,
which was developed in the private sector, on 0.05
million hectares in the year 2002.
ï¶In 2009, transgenic Bt cotton was cultivated by 5.6
million farmers on 8.6 million hectares.
ï¶India now occupies second position in terms of
global cotton production by turning out 35 million
bales of cotton in 2010.
21. 1- Genetically Modifiedingredients can cause cancer
âą A consultant histopathologist
Doctor- Stanley Ewan, raised
the concern that food and
water contaminated with
genetically engineered
material could increase the
growth of malignant tumor's
upon contact with humans.
22. 2. It couldraise new allergy outbreaks in humans
âą It was found that a
genetically engineered
soya bean containing
Brazilian nut protein was
allergenic to humans and
was withdrawn from
production
23. 3. Adverse effects on the immune system
âą A study in 2008 showed that
feeding mice engineered food for
30 days caused an immune
reaction and altered the number
of cells that regulated the immune
functions in the body
24. 4.Pesticide resistant foodcan create super-creatures
âą Itâs not a joke, modified genes
in engineered crops that resist
pesticides can transfer to
insects and pests; making them
pesticide resistant. These
super-organisms cannot be
controlled easily which will
create problems in managing
their numbers and to crop
production in future
25. CONCLUSION
Transgenic plants have the potential to
solve many of the world's hunger and
malnutrition problems, and to help
protect and preserve the environment
by increasing yield and reducing
reliance upon chemical pesticides and
herbicides.