The document discusses research on Fusarium head blight (FHB) in wheat. It outlines several key pillars for studying plant pathogens and developing control systems, including pathogen biology, detection and monitoring, antifungals, crop genetics and management. It then details the research group's long-term database on FHB, which links weather conditions, crop history and varieties to disease presence. This database forms the basis for their FHB prediction model. The document also discusses various FHB control strategies studied, including fungicides, biofumigation, crop rotation and breeding resistant varieties.
Prevalence, occurrence and biochemical characterization of Xanthomonas campes...INNS PUBNET
Xanthomonas campestris pv. vesicatoria the causal organism of bacterial spot in tomato results in heavy losses both in the form of quality and. In this study a survey was carried out to report the incidence of bacterial spot disease of tomato in district Swat. We reported maximum disease incidence in tehsil Kabal (71.66%), followed by Charbagh (61.66%) and Barikot (58.33%). For resistant screening a total of 13 tomato germplasms were screened against the disease. The foliar severity ranged from 3.33% to 73.33%, while severity for fruits was ranged from 18.33% to 30.66%. In case of phenotypic data the highest numbers of fruits obtained were 34, plant height 79.5cm and fruit weight was 470 grams/ten tomatoes. While the lowest average numbers of fruits were 6.67, plant height 45.7cm and fruit weight recorded was 215.67 grams/ten tomatoes. Line 1288 showed highest level of resistance followed by Red-stone. However, line 9708 showed highest susceptibility when exposed to artificial inoculation. Our study showed that bacterial spot is a major issue in some part of Pakistan and germplasm screening are linked to increased host resistance and could offer an important contribution to future integrated bacterial spot management programs.
Prevalence, occurrence and biochemical characterization of Xanthomonas campes...INNS PUBNET
Xanthomonas campestris pv. vesicatoria the causal organism of bacterial spot in tomato results in heavy losses both in the form of quality and. In this study a survey was carried out to report the incidence of bacterial spot disease of tomato in district Swat. We reported maximum disease incidence in tehsil Kabal (71.66%), followed by Charbagh (61.66%) and Barikot (58.33%). For resistant screening a total of 13 tomato germplasms were screened against the disease. The foliar severity ranged from 3.33% to 73.33%, while severity for fruits was ranged from 18.33% to 30.66%. In case of phenotypic data the highest numbers of fruits obtained were 34, plant height 79.5cm and fruit weight was 470 grams/ten tomatoes. While the lowest average numbers of fruits were 6.67, plant height 45.7cm and fruit weight recorded was 215.67 grams/ten tomatoes. Line 1288 showed highest level of resistance followed by Red-stone. However, line 9708 showed highest susceptibility when exposed to artificial inoculation. Our study showed that bacterial spot is a major issue in some part of Pakistan and germplasm screening are linked to increased host resistance and could offer an important contribution to future integrated bacterial spot management programs.
Presentation 8: Vibrio parahaemolyticus: a versatile pathogen that can adapt ...ExternalEvents
http://www.fao.org/documents/card/en/c/28b6bd62-5433-4fad-b5a1-8ac61eb671b1/
International Technical Seminar/Workshops on Acute hepatopancreatic necrosis disease (AHPND)
Using a naturally occurring virus to manage insects in turfgrass: Current sit...caprater
Presentation to Society of Invertebrate Pathologists detailing MS research as well as prospects and limitations to baculovirus as a biopesticide in turfgrass
The Edge of Tomorrow — Plant Health in the 21st CenturySophien Kamoun
Presented at the ICPP2018 International Congress of Plant Pathology Plenary Session - Plant Health is Earth’s Wealth, Monday, July 30, 2018. See notes and acknowledgments at http://kamounlab.tumblr.com/post/176385835530/the-edge-of-tomorrow-plant-health-in-the-21st
Los días 20 y 21 de mayo de 2014, la Fundación Ramón Areces organizó el Simposio Internacional 'Microorganismos beneficiosos para la agricultura y la protección de la biosfera' dentro de su programa de Ciencias de la Vida y de la Materia.
"New approaches to managing cabbage stem flea beetle in oilseed rape"EMPHASIS PROJECT
Cabbage stem flea beetle (CSFB) was an insignificant pest of oilseed rape years ago, but loss of neonicotenoid seed treatments has meant that it poses a serious threat to crop establishment. NIAB described a new approach using companion crops which may help to manage the pest in the future.
Speaker was NIAB researcher Simon Kightley.
The target audience are researchers, agri-business and forestry experts, farmers and foresters and any other interested in plant health.
Do not hesitate to contact EMPHASIS project through Facebook, Twitter, email or through their website if you want to be updated on webinars dates and content and book a ticket.
Presentation 3.3 Dealing with AHPND: Viet Nam experience (Dr Dang Thi Lua)ExternalEvents
http://www.fao.org/documents/card/en/c/28b6bd62-5433-4fad-b5a1-8ac61eb671b1/
FAO Second International Technical Seminar/Workshop on Acute hepatopancreatic necrosis disease (AHPND) There is a way forward! FAO Technical Cooperation Programme: TCP/INT/3501 and TCP/INT/3502.
Presentation 1.8 Surveillance and animal health monitoring, early detection o...ExternalEvents
http://www.fao.org/documents/card/en/c/28b6bd62-5433-4fad-b5a1-8ac61eb671b1/
FAO Second International Technical Seminar/Workshop on Acute hepatopancreatic necrosis disease (AHPND) There is a way forward! FAO Technical Cooperation Programme: TCP/INT/3501 and TCP/INT/3502.
☺INTRODUCTION
☺Bt COTTON
☺MAJOR PESTS OF COTTON
☺MODE OF ACTION OF Bt GENE
☺ADVANTAGES
☺DISADVANTAGES
☺CONCLUSION
☺REFERENCES
Genetically modified variety of cotton that produces an insecticide whose gene has been derived from a soil bacterium called Bacillus thuringiensis (Bt).
Three types of toxins.
A total of 229 cry toxins ( cry1Aa to Cry72Aa), cyt toxins ( cyt 11Aa to cyt3Aa) and 102 vip toxins( vip1Aa1 to vip4Aa1) have been discovered.
Presentation 8: Vibrio parahaemolyticus: a versatile pathogen that can adapt ...ExternalEvents
http://www.fao.org/documents/card/en/c/28b6bd62-5433-4fad-b5a1-8ac61eb671b1/
International Technical Seminar/Workshops on Acute hepatopancreatic necrosis disease (AHPND)
Using a naturally occurring virus to manage insects in turfgrass: Current sit...caprater
Presentation to Society of Invertebrate Pathologists detailing MS research as well as prospects and limitations to baculovirus as a biopesticide in turfgrass
The Edge of Tomorrow — Plant Health in the 21st CenturySophien Kamoun
Presented at the ICPP2018 International Congress of Plant Pathology Plenary Session - Plant Health is Earth’s Wealth, Monday, July 30, 2018. See notes and acknowledgments at http://kamounlab.tumblr.com/post/176385835530/the-edge-of-tomorrow-plant-health-in-the-21st
Los días 20 y 21 de mayo de 2014, la Fundación Ramón Areces organizó el Simposio Internacional 'Microorganismos beneficiosos para la agricultura y la protección de la biosfera' dentro de su programa de Ciencias de la Vida y de la Materia.
"New approaches to managing cabbage stem flea beetle in oilseed rape"EMPHASIS PROJECT
Cabbage stem flea beetle (CSFB) was an insignificant pest of oilseed rape years ago, but loss of neonicotenoid seed treatments has meant that it poses a serious threat to crop establishment. NIAB described a new approach using companion crops which may help to manage the pest in the future.
Speaker was NIAB researcher Simon Kightley.
The target audience are researchers, agri-business and forestry experts, farmers and foresters and any other interested in plant health.
Do not hesitate to contact EMPHASIS project through Facebook, Twitter, email or through their website if you want to be updated on webinars dates and content and book a ticket.
Presentation 3.3 Dealing with AHPND: Viet Nam experience (Dr Dang Thi Lua)ExternalEvents
http://www.fao.org/documents/card/en/c/28b6bd62-5433-4fad-b5a1-8ac61eb671b1/
FAO Second International Technical Seminar/Workshop on Acute hepatopancreatic necrosis disease (AHPND) There is a way forward! FAO Technical Cooperation Programme: TCP/INT/3501 and TCP/INT/3502.
Presentation 1.8 Surveillance and animal health monitoring, early detection o...ExternalEvents
http://www.fao.org/documents/card/en/c/28b6bd62-5433-4fad-b5a1-8ac61eb671b1/
FAO Second International Technical Seminar/Workshop on Acute hepatopancreatic necrosis disease (AHPND) There is a way forward! FAO Technical Cooperation Programme: TCP/INT/3501 and TCP/INT/3502.
☺INTRODUCTION
☺Bt COTTON
☺MAJOR PESTS OF COTTON
☺MODE OF ACTION OF Bt GENE
☺ADVANTAGES
☺DISADVANTAGES
☺CONCLUSION
☺REFERENCES
Genetically modified variety of cotton that produces an insecticide whose gene has been derived from a soil bacterium called Bacillus thuringiensis (Bt).
Three types of toxins.
A total of 229 cry toxins ( cry1Aa to Cry72Aa), cyt toxins ( cyt 11Aa to cyt3Aa) and 102 vip toxins( vip1Aa1 to vip4Aa1) have been discovered.
It is about the major pest of cabbage ways to tackle the pest in an organized manner with lesser environmental damage alongside with the help of IPM principles.
The Bt is a short form of ubiquitous soil bacterioum Bacillus thuringiensis. This bacterium is gram positive and spore forming that forms parasporal crystals during stationary phase of its growth cycle. The synthesized crystalline proteins called ‘endotoxins’ are highly toxic to certain insects. They kill the insect by acting on the epithelium tissues of midgut of caterpillars. These proteins are characterized by their insecticidal activity and are therefore grouped into four classes i.e. Lepidoptera-specific (Cry I), Lepidoptera and Diptera-specific (Cry II), Coleoptera-specific (Cry III) and Diptera-specific (Cry IV). Cotton bollworms belong to the order Lepidoptera and therefore are sensitive to Bt Cry I and Cry II proteins, which are specific to them. Other beneficial insects are unaffected by these proteins. popularly and effectively utilized are Cry 1 Ac, Cry 1 Ab in different crops.
Diallel mating in selected mustard genotypesfahimkhan163
It will help the researchers how to conduct diallel mating among mustard crops. This study also aims to screen out salinity tolerance and short duration mustard genotypes which will lead to develop a desired variety in future
This study was initiated in order to understand the biochemical mechanisms involved in the resistance of J. curcas to Lasiodiplodia theobromae. Artificial inoculations were done on young seedlings leaves of 8 J. curcas local accessions from Burkina Faso including 4 resistant and 4 susceptible. Two extractions were made for the evaluation of each biochemical parameter: one on the 1st day before and the second on the 7th day after inoculation. The study revealed that the response of J curcas to L. threobromae infection is depending to the resistance or the susceptibility of accessions. It showed an increase in the content of biomolecules synthesis such as phenolic, flavonoids, proteins, photosynthetic pigments, salicylic acid and also an increase of catalase and trypsin inhibition activities. In susceptible accessions, the study revealed a decrease in the photosynthetic pigments and an increase in MDA content comparatively to resistant accessions. The ACP performed on the basis of the evaluated biochemical parameters showed that the axis F1 allows the separation of the resistant and the susceptible accessions, and indicated that the resistance of accessions result in the activation of biomolecules synthesis such as phenolic, flavonoids, proteins, photosynthetic pigments, salicylic acid and stimulation of catalase and trypsin inhibition activities. The F2 axis is associated with sugars content and, chymotrypsin inhibition and SOD activities. In susceptible accessions, an increase in MDA content and SOD activity were observed. This study represents an important step in combating leaf fungal diseases by opting to the green technology and by breeding for genotypes with inducing natural defense compounds.
This presentation is to understand the concepts of endophytes that reside within plants & to explore the applications of endophytes for the management of plant diseases.
Morphological diversity, pathogenicity and biofungicides efficacity on Cercos...Open Access Research Paper
Fungal diseases are one of major constraints on groundnut production in Burkina Faso. Among these diseases, early leaf spot caused by Cercospora arachidicola (Hori.) is one of the most important economic diseases of groundnut. Aim of contributing to search effective control methods against this disease, we undertook the present study, which consisted in (i) study morphological diversity of different Cercospora arachidicola isolates (ii) study the level of pathogenicity of Cercospora arachidicola strains identified (iii) evaluate the efficacy of some bio-fungicides on the strains identified. The study was carried out in 14 villages in the Hauts Bassins and Boucle du Mouhoun regions of Burkina Faso with regard to prospecting and sample collection, and at the INERA Bobo Dioulasso plant pathology laboratory for isolation, identification, pathogenicity and biofungicide efficacy testing. A total of five strains of Cercospora arachidicola were identified. The pathogenicity test was used to classify the five strains according to their virulence. In decreasing order of virulence, the strains were Fara, Darsa, Logo, Santi and Kod. In vitro evaluation of biofungicide efficacy shows that PLANSAIN biofungicide provides better control of Cercospora arachidicola strains. Trichoderma hazanium, the active ingredient in PLANSAIN, inhibits the radial growth of Cercospora arachidicola strains to a greater extent.
Ang Chong Yi Navigating Singaporean Flavors: A Journey from Cultural Heritage...Ang Chong Yi
In the heart of Singapore, where tradition meets modernity, He embarks on a culinary adventure that transcends borders. His mission? Ang Chong Yi Exploring the Cultural Heritage and Identity in Singaporean Cuisine. To explore the rich tapestry of flavours that define Singaporean cuisine while embracing innovative plant-based approaches. Join us as we follow his footsteps through bustling markets, hidden hawker stalls, and vibrant street corners.
At Taste Of Middle East, we believe that food is not just about satisfying hunger, it's about experiencing different cultures and traditions. Our restaurant concept is based on selecting famous dishes from Iran, Turkey, Afghanistan, and other Arabic countries to give our customers an authentic taste of the Middle East
Roti Bank Hyderabad: A Beacon of Hope and NourishmentRoti Bank
One of the top cities of India, Hyderabad is the capital of Telangana and home to some of the biggest companies. But the other aspect of the city is a huge chunk of population that is even deprived of the food and shelter. There are many people in Hyderabad that are not having access to
1. 25.06.2019
1
Geert Haesaert
Faculty of Bioscience Engineering
Ghent University
Geert.Haesaert@ugent.be
Wat are important pillars when studying a
plant pathogen and for developing an
adequate control system?
• Pathogen biology
• Pathogen detection and
monitoring
• Antifungals/remediation
strategies
• Interaction with crop genotype
and crop husbandry
• …
(adapted from Bebber and Gurr (2015) Fungal Genetics and Biology):
2. 25.06.2019
2
• Started at the beginning of 2000
• Following up for FHD and DON at least
20 fields each year
o Weather conditions, crop history,
varieties, crop management, …
o Fusarium species in FHB population
(qPCR)
• Fungicide trials/other FHB control
techniques
• Varietal susceptibility/tolerance
• ….
Fusarium research in relation to FHB at our
department Plants and crops (1)
Fusarium research in relation to FHB at our
department plants and crops (2)
• Database of more than 7000 data points
o Relation between crop management/ weather
conditions/varieties and presence of FHB
o Database is the basis for our prediction model
7
7
11
23
7
6
23
21
17
13
15
12
40
18
24
16
16
13
16
40
18
15
21
14
15
16
21
18
33
22
33
22
33
33
33
33
33
35
24
36
24
36
48
48
36
36
27
27
27
27
27
27
27
27
27
48
48
48
24
48
48
49
48
48
68
26
56
26
52
52
60
56
56
48
24
48
24
48
48
48
48
48
44
44
44
16
44
44
40
44
44
48
16
48
16
12
44
48
48
64
48
64
48
64
16
60
64
64
40
40
30
40
16
40
40
40
72
54
76
74
74
78
74
76
67
64
64
64
64
15
64
54
68
67
67
67
14
64
67
67
Assent
Bottelare
Helkijn
Huldenberg
Koksijde
Nieuwenhove
Poperinge
Tongeren
Verrebroek
Zuienkerke
Zwevegem
2002
2003
2004
2005
2006
2007
2008
2009
2010
2011
2012
2013
2014
2015
2016
2017
2018
l
20
40
60
3. 25.06.2019
3
0 20 40 60 80
0.00.51.01.52.02.5
2003
2004
2006
2007
2008
2009
2010
2011
overall
DI (%)
DONcontent(mg/kg)
Fusarium species: cereal killers with much
yearly variability in epidemiology
2002
2003
2004
2005
2006
2007
2008
2009
2010
2011
2012
2013
2014
2015
2016
2017
01234
DONcontent(mg/kg)
2003
2004
2006
2007
2008
2009
2010
2011
2012
2013
2014
2015
2016
2017
020406080
Diseaseindex(%)
2002
2003
2004
2005
2006
2007
2008
2009
2010
2011
2012
2013
2014
2015
2016
2017
2018
%
020406080100
M. nivale
F. poae
F. graminearum
F. culmorum
F. avenaceum
Fusarium species: cereal
killers with much yearly
variability in species ratio
within FHB population
4. 25.06.2019
4
How to control FHB?
Prevention
Cultural, breeding for
resistance, …
Biological,
mechanical,
physiological
ChemicalChemical
Intervention/suppression
Prevention
Monitoring - prediction
Avoiding
Integrated disease management
KNOWLEDGE
5. 25.06.2019
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Crop Rotation: influence on DON accumulation
-58%
-42%
-88%
Crop rotation + soil management
Experiment design
First crop maize:
o harvest as grain
and as silage
o two different
varieties (S /T)
Second crop: winter
wheat
o Plowing or
inverse soil
management
o 5 different wheat
varieties
DON in
winter
wheat
Kruskall wallis test
Plowing/no plowing grain /silage two maize varieties wheat varieties
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6
Bio-fumigation: best know system is with Brassicaceae
o Glucosinolates are converted to isothiocyanate after cells are
mechanically damaged
o ITC = volatile
o Myrosinase activity is t° and pH depended!
Cultural: bio-
fumigationCrop Rotation: cover crops and bio-fumigations
10%
14%
-7% -3%
7%
3% 2%
6%
-1%
18% *
12%
16% *
-6% 0%
53% *
98% * 95% *
-1%
25% *
-10%
10%
30%
50%
70%
90%
GMC
GML
GMME
GMMI
GMPA
GMPO
GMSA
GMSE
GMSI
BRB
BRC
BRDA
BRDE
BRT
SM
SME
SMT
GRAS
EM
Reductiet.o.v.decontrolebehandeling
Synapsis alba
Raphanus sativus
Brassica juncea
Brassica carinata
F. graminearum
C R. sativus S. alba B. carinata B. Juncea
Reduction of mycelium growth
o Inverted petri dish method: volatiles
Crop Rotation: cover crops and bio-fumigations
7. 25.06.2019
7
Biofumigation: dose effect
a a a
b
ab
b
c
bc
bab
cd
b
d
d
c
0
500
1000
1500
2000
2500
5 gram 10 gram 15 gram
Oppervlakte(mm²)
CONTROLE GMC BRB GRAS SM
Cultural: bio-
fumigation
Reduction of mycelium growth
Crop Rotation: cover crops and bio-fumigations
Abbr. Crop Var.
AITC1-5 / /
LM L. multiflorum Meritra
SA… S. alba /
RS… R. sativus /
BC B. carinata Cappucino
BJT B. juncea Terrafit
BJV B. juncea Vitamine
BJE B. juncea Etamine
Crop Rotation: cover crops and bio-fumigations
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Bio-fumigation demands:
o Harvesting at the right time (maximum concentration of glucosinolates)
o You need biomass!
o A good incorporation of crop biomass
o Pressing of upper layers of soil
Crop Rotation: cover crops and bio-fumigations
• 10 biofumigation crops
included in a cover crop
experiment
oAugust 2018: sowing
• December 2018: first harvest and in
vitro experiment: fungal activity
• March 2019: second harvest and in
vitro experiment
Crop Rotation: cover crops and bio-fumigations
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Crop Rotation: cover crops and bio-fumigations
Abbr. Crop Var.
CTRL / /
SAL S. alba Lopex
SAME S. alba Meringue
SASA S. alba Salsa
RSDE R. sativus Defender
RSDO R. sativus Doublet BCA
RST R. sativus Terranova
BC B. carinata Cappucino
BJT B. juncea Terrafit
BJV B. juncea Vitamine
BJE B. juncea Etamine
Harvest December
Crop Rotation: cover crops and bio-fumigations
B. carinata
B. juncea
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Varieties
• Since 2005 we test more than 30 winter
wheat varieties each year
o Artificial infection (two times) with
mixture of different Fusarium
species
o DON and disease index is
determinated
o List with 4 classes is published every
year
Gevoelig Matig gevoelig Matig tolerant Tolerant
Alsace Azzerti Altigo Akteur
Anthus Belcast Altos Apache
Caphorn Biscay Armada Carenius
Gedser Cellule Atomic Evina
Lexus Challenger Avatar Henrik
Lithium Deben Benchmark KWS Smart
Mosaïc Dekan Bergamo Melkior
Nemocart Edgar Bodecor Omart
Oakley Einstein Britannia RGT Reform
Robigus Evasion Celebration Winnetou
Rosario Expert Centenaire Zohra
Tobak Florett Contender
Toisondor Forum Corvus
Tuscan Graham Elixer
Homeros Faustus
Horatio Folklor
Hyperion Fructidor
Istabraq Hattrick
JB Asano Hourra
JB Diego Impression
Katart Inspiration
KWS Siskin Intro
Linus Julius
Manager Kaspart
Mulan Koreli
Ozon Lear
Pepidor Limes
Premio Lincoln
Razzano Lion
Rollex Louisart
Scor Matrix
Selekt Mentor
Smuggler Ortop
SW Tataros Patrel
Terroir Popeye
Vivant Profilus
Waldorf Pulsar
Radius
RGT Sacramento
Rubisko
Sahara
Spirit
Tabasco
Tulsa
Unicum
Valdo
Vasco
Climatic risk = rainfall
flowering
Previous crop
Low High
99% <
1000µg/kg
89% <
1000µg/kg
Previous crop
HighLow
Ploughing
93% <
1000µg/kg
89% <
1000µg/kg
Yes No
Ploughing
74% <
1000µg/kg
38% <
1000µg/kg
NoYes
wet ear period
must be at least
72 h
RAS: 54%<
1000µg/kg
RAS: 92 % <
1000µg/kg
RAS: 93 % <
1000µg/kg
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Varieties
No evolution in susceptibility/tolerance of genotypes on
Belgian market
Interaction with ohter
organisms: grass weeds
Grass species are
alternative host plants
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12
Interaction with ohter
organisms: aphids
mg kg-1 Fg Fg+aphids P-value
DON 32.77 ± 12.73 a 65.83 ± 20.15 b 0.031
3-ADON 0.53 ± 0.20 a 2.05 ± 0.83 b 0.023
15-ADON 2.39 ± 0.96 a 9.60 ± 3.88 b 0.019
Fungicides
Fungicides are not the holy grail …..
Fungicides: reduction of DON
• Fungicide experiments from
2002-2018
• At least 4 experiments per
year
Arvalis, France
13. 25.06.2019
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Fungicides
Fungicides are not the holy grail …..
isolaat 10
0
20
40
60
80
100
cont role 1mg/l 5 mg/l 10 mg/ l 50 mg/ l 500mg/l
prothioconazool
4 h
24 h
48 h
F. poae
F. graminearum
Fungicides
Fungicides are not the holy grail …..
FHB population vs fungicide use
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InterventionFungicides
Only 3 active ingredients are effective againgst Fusarium
species
- Tebuconazole
- Metconazole
- Prothioconazole
Holistic:
o based on data-mining; data including weather, field characteristics
and crop history, variety, dynamics of pathogen population, etc.
o Orthogonal regression model
o Cross-validation
DON prediction is available for Flemish farmers
Prediction model
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COMPARISON OF PREDICTIONS AT THE END OF THE
GROWING SEASON
AND MEASURED DON VALUES (2017)
<0.2mg/kg
0.2-0.5mg/kg
0.5-0.75mg/kg
>0.75mg/kg
Koksijde
Percentage
04080
Predicted
Measured
<0.2mg/kg
0.2-0.5mg/kg
0.5-0.75mg/kg
>0.75mg/kg
Poperinge
Percentage
04080
Predicted
Measured
<0.2mg/kg
0.2-0.5mg/kg
0.5-0.75mg/kg
>0.75mg/kg
Kortrijk
Percentage
04080
Predicted
Measured
<0.2mg/kg
0.2-0.5mg/kg
0.5-0.75mg/kg
>0.75mg/kg
Bottelare
Percentage
04080
Predicted
Measured
<0.2mg/kg
0.2-0.5mg/kg
0.5-0.75mg/kg
>0.75mg/kg
Verrebroek
Percentage
04080
Predicted
Measured
<0.2mg/kg
0.2-0.5mg/kg
0.5-0.75mg/kg
>0.75mg/kg
Nieuwenhove
Percentage
04080
Predicted
Measured
Prevention
Screening for endophytes
Alternatives for fungicides
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Isolation and characterization of Piriformospora
1. Sampling in Kisangani, DRCongo
3. Isolation4. Morphological and molecular characterization of 51 isolates
obtained from 6 different locations in the Kisangani region
2. Trapping Piriformospora by the use of a soil-based system
with Sorghum
1 12 24 30 34 36 46 PI PW
Venneman Jolien – 17/11/2017
Molecular
analysis:
biodiversity
Prevention
In vitro testing endophytes
F. Graminearum + Piriformospora /// F. Graminearum
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FUS + mycelium isolate 30
FUS control (without
autoclaved mycelium)
Bio-control effects – greenhouse trials
Inoculation with Fusarium-infected rice kernels
Plant fresh weight of Fusarium-inoculated maize plantsRoot fresh weight of Fusarium-inoculated maize plantsPlant survival
Prevention
Fusarium
cbs266
cbs243
cbs1005
cbs102-94
cbs1004
IP80
PW
Piri34-36
Piri23-K1
Piri15-6
PI
Piri5-A
Piri23-18
EpiNigrum1
EpiNigrum2
EpiNigrum3
Bionectria
Bio2
C.rosaBE
Sordaria1
SordariaMUCL
0100030005000
DON
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CBS266
CBS243
CBS1005
CBS102_94
CBS1004
IP80
PI
PW
piri34_36
Piri23_K1
Piri15_6
Piri17_A
Piri5_A
Piri23_18
EpiNigrum1
EpiNigrum2
EpiNigrum3
Bionectria
Bio2
C.rosaBE
Sordaria1
SordariaMUCL
050100150200
Relativegrowth
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Screening for endophytes Flanders starting from
maize residue’s
Sordaria sp.
Epicoccum sp.
Clonostachus sp. Clonostachus sp.
Epicoccum sp.
Sordaria sp.