01 Presentation I VS (8-55MB)- (3-28-08).ppsvshidham
Part I of Four part symposium: “Diagnostic Cytopathology of Serous Effusion” on April 19, 2007 at Neenah, WI, USA
(2008 Wisconsin Society of Cytology, 40th Anniversary)
03 Presentations III VS (8-47MB)- (3-28-08).ppsvshidham
Part III of Four part symposium: “Diagnostic Cytopathology of Serous Effusion” on April 19, 2007 at Neenah, WI, USA
(2008 Wisconsin Society of Cytology, 40th Anniversary)
02 Presentations Ii Vs (14 4 Mb) (3 30 08)vshidham
Part II of Four part symposium: “Diagnostic Cytopathology of Serous Effusion” on April 19, 2007 at Neenah, WI, USA
(2008 Wisconsin Society of Cytology, 40th Anniversary)
color atlas on bethesda system for reporting thyroid cytologyAshish Jawarkar
this is a color atlas on bethesda system for reporting thyroid cytology. there are nearly 300 images in atlas with explanatory text which will help students and practitioners alike. All images are taken from pap society web atlas.. and entire credit for this work should go to the society.. I have put together images available at one place..
THIS IS A PREVIEW ONLY..ENTIRE DOCUMENT IS AVAILABLE ON SCRIBD.. LINK PROVIDED IN DOCUMENT
This document discusses cell blocks, liquid based cytology, and the Bethesda system for reporting cervical cytology. It defines cell blocks and describes their advantages like allowing histological examination and ancillary tests. It outlines the advantages and disadvantages of liquid based cytology compared to conventional pap smears. Finally, it explains the Bethesda system for standardized reporting of cervical cytology results, including categories for negative, epithelial abnormalities, organisms, and other non-neoplastic findings.
Bronchial cytology and fine needle aspiration are important techniques for diagnosing lung lesions. Bronchial cytology examines the central airways using bronchoscopy while fine needle aspiration can sample peripheral lung lesions. Normal respiratory cells include ciliated bronchial cells and alveolar macrophages. Benign lesions may show goblet cell or basal cell hyperplasia. Non-neoplastic lung diseases like sarcoidosis and pulmonary alveolar proteinosis can also be diagnosed through their characteristic cytology findings. Cytology is useful for identifying infection causes as well as diagnosing and classifying lung tumors.
This is a presentation on the topic of cytology of the breast, prepared by Dr Ashish Jawarkar, he is MD in pathology and a teacher at Parul institute of Medical sciences and research Vadodara.
The document discusses the thyroid FNA procedure and diagnostic categories. It provides details on:
- Performing thyroid FNA under ultrasound guidance using a 25 gauge needle with 3-4 passes.
- Preparing direct smears, cytospins, cell blocks and liquid-based preparations from the aspirated material.
- The Bethesda system for reporting thyroid cytopathology which includes 6 diagnostic categories and their associated cancer risks to guide clinical management.
- Key cytologic features that help diagnose common thyroid lesions and cancers.
01 Presentation I VS (8-55MB)- (3-28-08).ppsvshidham
Part I of Four part symposium: “Diagnostic Cytopathology of Serous Effusion” on April 19, 2007 at Neenah, WI, USA
(2008 Wisconsin Society of Cytology, 40th Anniversary)
03 Presentations III VS (8-47MB)- (3-28-08).ppsvshidham
Part III of Four part symposium: “Diagnostic Cytopathology of Serous Effusion” on April 19, 2007 at Neenah, WI, USA
(2008 Wisconsin Society of Cytology, 40th Anniversary)
02 Presentations Ii Vs (14 4 Mb) (3 30 08)vshidham
Part II of Four part symposium: “Diagnostic Cytopathology of Serous Effusion” on April 19, 2007 at Neenah, WI, USA
(2008 Wisconsin Society of Cytology, 40th Anniversary)
color atlas on bethesda system for reporting thyroid cytologyAshish Jawarkar
this is a color atlas on bethesda system for reporting thyroid cytology. there are nearly 300 images in atlas with explanatory text which will help students and practitioners alike. All images are taken from pap society web atlas.. and entire credit for this work should go to the society.. I have put together images available at one place..
THIS IS A PREVIEW ONLY..ENTIRE DOCUMENT IS AVAILABLE ON SCRIBD.. LINK PROVIDED IN DOCUMENT
This document discusses cell blocks, liquid based cytology, and the Bethesda system for reporting cervical cytology. It defines cell blocks and describes their advantages like allowing histological examination and ancillary tests. It outlines the advantages and disadvantages of liquid based cytology compared to conventional pap smears. Finally, it explains the Bethesda system for standardized reporting of cervical cytology results, including categories for negative, epithelial abnormalities, organisms, and other non-neoplastic findings.
Bronchial cytology and fine needle aspiration are important techniques for diagnosing lung lesions. Bronchial cytology examines the central airways using bronchoscopy while fine needle aspiration can sample peripheral lung lesions. Normal respiratory cells include ciliated bronchial cells and alveolar macrophages. Benign lesions may show goblet cell or basal cell hyperplasia. Non-neoplastic lung diseases like sarcoidosis and pulmonary alveolar proteinosis can also be diagnosed through their characteristic cytology findings. Cytology is useful for identifying infection causes as well as diagnosing and classifying lung tumors.
This is a presentation on the topic of cytology of the breast, prepared by Dr Ashish Jawarkar, he is MD in pathology and a teacher at Parul institute of Medical sciences and research Vadodara.
The document discusses the thyroid FNA procedure and diagnostic categories. It provides details on:
- Performing thyroid FNA under ultrasound guidance using a 25 gauge needle with 3-4 passes.
- Preparing direct smears, cytospins, cell blocks and liquid-based preparations from the aspirated material.
- The Bethesda system for reporting thyroid cytopathology which includes 6 diagnostic categories and their associated cancer risks to guide clinical management.
- Key cytologic features that help diagnose common thyroid lesions and cancers.
1. The patient presented with intermittent right upper abdominal pain for 2 years with associated low grade fever. Imaging showed multiple hydatid cysts in the liver.
2. Hydatid cyst disease is caused by the larval stage of Echinococcus granulosus. Humans can become infected by contact with definitive hosts like dogs or ingesting contaminated food or water.
3. Treatment options include albendazole medication, puncturing and draining cysts using PAIR technique, or surgery to remove cysts depending on number and location.
The bethesda system for reporting thyroid cytopathology dhanya89
The Bethesda System for Reporting Thyroid Cytopathology document outlines the standardized categories for reporting thyroid fine needle aspiration biopsy results. The categories include: Nondiagnostic/Unsatisfactory, Benign, Atypia of Undetermined Significance/Follicular Lesion of Undetermined Significance, Follicular Neoplasm/Suspicious for Follicular Neoplasm, Suspicious for Malignancy, and Malignant. Each category has specific criteria for cellularity, architecture, and nuclear features to provide consistent terminology for thyroid FNA interpretation and patient management.
The 2016 WHO Classification of Renal Neoplasia contains significant changes from the 2004 classification. Key changes include:
1) Renal tumor subtypes are now defined based on features such as cytoplasmic appearance, architecture, anatomical location, associated diseases, and molecular alterations.
2) Multilocular cystic renal cell carcinoma is now termed "multilocular cystic renal neoplasm of low malignant potential" based on its indolent behavior.
3) Papillary renal cell carcinoma subtypes (type 1 and 2) are still recognized but type 2 is heterogeneous and oncocytic variants are not a distinct entity. Papillary adenomas are now defined as ≤1.5 cm.
Several
Hydatid cyst disease of the liver الدكتور طارق المنيزل Tariq Al munaizel
Echinococcus granulosus is a parasitic tapeworm that causes hydatid disease (hydatidosis) in humans. Its life cycle involves carnivores as definitive hosts and herbivores as intermediate hosts. Humans can be infected by ingesting E. granulosus eggs from a definitive host. The larvae develop into hydatid cysts, most commonly in the liver. Hydatid cysts can cause symptoms from pressure or complications like rupture. Diagnosis involves imaging and serology. Treatment depends on cyst type and complications but may include surgery, percutaneous drainage, or antiparasitic drugs.
This slide presentation summarizes a cytology case involving a 38-year-old female patient. A cervical smear shows atypical cells with dense, scanty cytoplasm and enlarged hyperchromatic nuclei throughout the smear. Background shows sheets of neutrophils, coccobacilli and hemorrhage. The specimen is categorized as an epithelial cell abnormality and interpreted as a high-grade squamous intra-epithelial lesion. Colposcopic examination is suggested. The presentation provides further details on cytology techniques, classifications of abnormal cervical cells, screening guidelines, and characteristics of low and high-grade squamous lesions.
This document discusses liquid based cytology (LBC) and compares it to conventional pap smears. It provides a history of LBC dating back to 1970 and describes two main LBC systems - ThinPrep and SurePath. With ThinPrep, cells are collected in a preservative fluid and then filtered and transferred to a slide. With SurePath, cells are collected in a preservative fluid and separated via density gradient centrifugation before being placed on a slide. Studies have shown LBC reduces inadequate samples and increases specificity compared to conventional pap smears. LBC also better preserves cell morphology. The document focuses on how LBC has advantages over conventional pap smears for detecting cervical intraepithelial neoplasia lesions.
The document summarizes hydatid cyst disease, which is caused by the larva of the dog tapeworm Echinococcus granulosus. It affects the liver in most cases, presenting as a cyst with three layers. Diagnosis involves imaging like ultrasound and CT scan to identify cyst characteristics. Serological tests can also be used. Treatment options include medical therapy with antihelmintic drugs, percutaneous drainage and injection of cyst contents (PAIR procedure), or surgery depending on cyst location and size.
This document summarizes research on the morphological evaluation of atypical squamous cells of undetermined significance (ASC-US) in cervical cytology samples. It identifies six cytological patterns associated with ASC-US interpretations and correlates them with biopsy and HPV test results. These patterns include MGH-like, repair-like, atrophy-like single cells, atrophy-like parabasal cell groups, ASC-US not otherwise specified, and small atypical parakeratotic cells. The document also discusses cases showing low-grade squamous intraepithelial lesions (LSIL) co-existing with ASC-US features.
This document provides guidelines for renal biopsy techniques and analysis. It discusses sample size and location requirements, preferred fixation and staining methods for light microscopy, immunofluorescence, and electron microscopy. Key points covered include obtaining a minimum of 25 glomeruli for focal lesions and 7 for transplants. Juxtamedullary glomeruli are preferred since they are earliest involved in FSGS. Fixation methods and optimal stains for assessing features like cellularity, fibrosis, immune complexes, and basement membranes are also outlined. Categories of active versus fibrosing injury and examples of minimal change disease, membranous nephropathy and FSGS under light and electron microscopy are summarized.
Efficacy of liquid based cytology versus conventional smearsAnamika Dev
This study compares the efficacy of liquid-based cytology (LBC) to conventional smear methods for fine needle aspiration cytology samples. The study analyzed 110 cases of various lesions collected over 2 years. Results showed that LBC produced more clear backgrounds and better preserved and dispersed cells compared to conventional smears. LBC allowed for improved diagnosis of lesions like ductal carcinoma, lymph node metastases, and bone lesions. However, LBC required more processing and had some artifacts that could complicate interpretation. Overall, LBC improved sample quality and reduced inadequate sampling compared to conventional smears.
Hydatid cyst of the liver is caused by infection with the larval stage of Echinococcus tapeworms. The cysts grow slowly over years and can reach large sizes. Symptoms vary depending on cyst location and complications. Diagnosis involves blood tests, imaging like ultrasound or CT, and serologic tests. Treatment options include medical therapy with albendazole, percutaneous drainage with scolicidal agents (PAIR procedure), or surgical removal of cysts. Surgery aims to remove all cyst components while avoiding spillage, and techniques depend on cyst location and number. Postoperative medical treatment helps prevent recurrence. Long term follow up with imaging and serology is needed to monitor for recurrence.
Reporting thyroid fine needle aspiration by the bethesda systemMonika Nema
This document summarizes guidelines for thyroid fine needle aspiration (FNA) cytopathology reports. It discusses classifications including nondiagnostic/unsatisfactory, benign thyroid lesions, atypia of undetermined significance/follicular lesion of undetermined significance, and includes examples of diagnostic criteria, imaging features and recommended reporting language for each classification. Thyroid FNA is presented as an accurate and cost-effective initial test for evaluating thyroid nodules that can help determine if surgery is needed.
Liquid biopsy quality control – the importance of plasma quality, sample prep...Thermo Fisher Scientific
Liquid biopsy is emerging as a non-invasive companion to traditional solid tumor biopsies. As next generation sequencing (NGS) of circulating cell-free nucleic acids (cfNA = cfDNA and cfRNA) becomes common, it’s important to understand the impact of sample preparation on quality, specificity, and sensitivity of liquid biopsy tests. Plasma samples are often limited, and may have undesirable characteristics such as lipemia or hemolysis that contribute unwanted genomic DNA (gDNA) to the sample. Low cfDNA concentration can also limit the amount available for NGS library prep. In this study, we explore the effects of suboptimal plasma and low library input on liquid biopsy NGS, and discuss various techniques for in-process quality control of cfNA samples isolated from plasma
The document discusses the development and benefits of the Milan System for Reporting Salivary Gland Cytopathology. It aims to standardize terminology for salivary gland FNA reports which previously lacked uniformity. The system categorizes specimens as non-diagnostic, non-neoplastic, atypia of undetermined significance, neoplastic (benign or uncertain malignant potential), suspicious for malignancy, or malignant. It is intended to improve communication between pathologists and clinicians, enhance patient care, and facilitate research by allowing standardized data collection across institutions. While validation is ongoing, the system provides a practical framework for uniform reporting of salivary gland cytology.
1) This study analyzed the biliary anatomy of the caudate lobe in 500 living liver donors through intraoperative cholangiography.
2) It identified an average of 2.98 caudate ducts per donor and classified duct drainage patterns, finding left-to-right crossover ducts in 61.37% of cases.
3) Understanding caudate lobe anatomy through this classification aims to minimize biliary complications in both donors and recipients by guiding surgical technique and leak management.
Cervical cytology was introduced by George
Papanicolaou into clinical practice in 1940. In 1945,
the Papanicolaou smear received the endorsement of
the American cancer society as an effective method
for the prevention of cervical cancer .
www.drvikramsaraswat.co.in
www.drsaraswatpathlabs.com
Atlas on bethesda system for reporting cervical cytologyAshish Jawarkar
This is an atlas with more nearly 100 images, authentic taken from NCI web atlas. Useful to understand and report pap smears. The subject has been presented in a way which will help students reproduce in exams.
Accuracy and reliability_of_tzanck_test_compared_t (1)Eva Yustiana
This document compares the accuracy of the Tzanck test to histopathology for diagnosing basal cell carcinoma (BCC). The Tzanck test is a simple cytological examination that is faster and cheaper than histopathology, the gold standard. The study examined 26 patients clinically diagnosed with BCC using both tests. Histopathology confirmed BCC in 23 cases. The Tzanck test correctly identified BCC in 12 cases and ruled out the 3 non-BCC cases, but missed diagnosing BCC in 11 cases. The Tzanck test had a sensitivity of 52.2% and specificity of 100% compared to histopathology. While the Tzanck test can rapidly identify some BCC, it has
This document outlines an educational presentation on interpreting atypical squamous cells of undetermined significance (ASC-H) cervical cytology samples and related diagnoses. The presentation aims to describe the cytomorphological spectrum of ASC-H, define low-grade squamous intraepithelial lesion-cannot exclude high-grade (LSIL-H), discuss differential diagnoses of hyperchromatic crowded groups, and apply ancillary tests. It is divided into three sections, with the first focusing on morphological evaluation, the second on study cases using two-color immunochemistry, and the third on quality improvement, HPV testing, and medicolegal aspects.
1. The patient presented with intermittent right upper abdominal pain for 2 years with associated low grade fever. Imaging showed multiple hydatid cysts in the liver.
2. Hydatid cyst disease is caused by the larval stage of Echinococcus granulosus. Humans can become infected by contact with definitive hosts like dogs or ingesting contaminated food or water.
3. Treatment options include albendazole medication, puncturing and draining cysts using PAIR technique, or surgery to remove cysts depending on number and location.
The bethesda system for reporting thyroid cytopathology dhanya89
The Bethesda System for Reporting Thyroid Cytopathology document outlines the standardized categories for reporting thyroid fine needle aspiration biopsy results. The categories include: Nondiagnostic/Unsatisfactory, Benign, Atypia of Undetermined Significance/Follicular Lesion of Undetermined Significance, Follicular Neoplasm/Suspicious for Follicular Neoplasm, Suspicious for Malignancy, and Malignant. Each category has specific criteria for cellularity, architecture, and nuclear features to provide consistent terminology for thyroid FNA interpretation and patient management.
The 2016 WHO Classification of Renal Neoplasia contains significant changes from the 2004 classification. Key changes include:
1) Renal tumor subtypes are now defined based on features such as cytoplasmic appearance, architecture, anatomical location, associated diseases, and molecular alterations.
2) Multilocular cystic renal cell carcinoma is now termed "multilocular cystic renal neoplasm of low malignant potential" based on its indolent behavior.
3) Papillary renal cell carcinoma subtypes (type 1 and 2) are still recognized but type 2 is heterogeneous and oncocytic variants are not a distinct entity. Papillary adenomas are now defined as ≤1.5 cm.
Several
Hydatid cyst disease of the liver الدكتور طارق المنيزل Tariq Al munaizel
Echinococcus granulosus is a parasitic tapeworm that causes hydatid disease (hydatidosis) in humans. Its life cycle involves carnivores as definitive hosts and herbivores as intermediate hosts. Humans can be infected by ingesting E. granulosus eggs from a definitive host. The larvae develop into hydatid cysts, most commonly in the liver. Hydatid cysts can cause symptoms from pressure or complications like rupture. Diagnosis involves imaging and serology. Treatment depends on cyst type and complications but may include surgery, percutaneous drainage, or antiparasitic drugs.
This slide presentation summarizes a cytology case involving a 38-year-old female patient. A cervical smear shows atypical cells with dense, scanty cytoplasm and enlarged hyperchromatic nuclei throughout the smear. Background shows sheets of neutrophils, coccobacilli and hemorrhage. The specimen is categorized as an epithelial cell abnormality and interpreted as a high-grade squamous intra-epithelial lesion. Colposcopic examination is suggested. The presentation provides further details on cytology techniques, classifications of abnormal cervical cells, screening guidelines, and characteristics of low and high-grade squamous lesions.
This document discusses liquid based cytology (LBC) and compares it to conventional pap smears. It provides a history of LBC dating back to 1970 and describes two main LBC systems - ThinPrep and SurePath. With ThinPrep, cells are collected in a preservative fluid and then filtered and transferred to a slide. With SurePath, cells are collected in a preservative fluid and separated via density gradient centrifugation before being placed on a slide. Studies have shown LBC reduces inadequate samples and increases specificity compared to conventional pap smears. LBC also better preserves cell morphology. The document focuses on how LBC has advantages over conventional pap smears for detecting cervical intraepithelial neoplasia lesions.
The document summarizes hydatid cyst disease, which is caused by the larva of the dog tapeworm Echinococcus granulosus. It affects the liver in most cases, presenting as a cyst with three layers. Diagnosis involves imaging like ultrasound and CT scan to identify cyst characteristics. Serological tests can also be used. Treatment options include medical therapy with antihelmintic drugs, percutaneous drainage and injection of cyst contents (PAIR procedure), or surgery depending on cyst location and size.
This document summarizes research on the morphological evaluation of atypical squamous cells of undetermined significance (ASC-US) in cervical cytology samples. It identifies six cytological patterns associated with ASC-US interpretations and correlates them with biopsy and HPV test results. These patterns include MGH-like, repair-like, atrophy-like single cells, atrophy-like parabasal cell groups, ASC-US not otherwise specified, and small atypical parakeratotic cells. The document also discusses cases showing low-grade squamous intraepithelial lesions (LSIL) co-existing with ASC-US features.
This document provides guidelines for renal biopsy techniques and analysis. It discusses sample size and location requirements, preferred fixation and staining methods for light microscopy, immunofluorescence, and electron microscopy. Key points covered include obtaining a minimum of 25 glomeruli for focal lesions and 7 for transplants. Juxtamedullary glomeruli are preferred since they are earliest involved in FSGS. Fixation methods and optimal stains for assessing features like cellularity, fibrosis, immune complexes, and basement membranes are also outlined. Categories of active versus fibrosing injury and examples of minimal change disease, membranous nephropathy and FSGS under light and electron microscopy are summarized.
Efficacy of liquid based cytology versus conventional smearsAnamika Dev
This study compares the efficacy of liquid-based cytology (LBC) to conventional smear methods for fine needle aspiration cytology samples. The study analyzed 110 cases of various lesions collected over 2 years. Results showed that LBC produced more clear backgrounds and better preserved and dispersed cells compared to conventional smears. LBC allowed for improved diagnosis of lesions like ductal carcinoma, lymph node metastases, and bone lesions. However, LBC required more processing and had some artifacts that could complicate interpretation. Overall, LBC improved sample quality and reduced inadequate sampling compared to conventional smears.
Hydatid cyst of the liver is caused by infection with the larval stage of Echinococcus tapeworms. The cysts grow slowly over years and can reach large sizes. Symptoms vary depending on cyst location and complications. Diagnosis involves blood tests, imaging like ultrasound or CT, and serologic tests. Treatment options include medical therapy with albendazole, percutaneous drainage with scolicidal agents (PAIR procedure), or surgical removal of cysts. Surgery aims to remove all cyst components while avoiding spillage, and techniques depend on cyst location and number. Postoperative medical treatment helps prevent recurrence. Long term follow up with imaging and serology is needed to monitor for recurrence.
Reporting thyroid fine needle aspiration by the bethesda systemMonika Nema
This document summarizes guidelines for thyroid fine needle aspiration (FNA) cytopathology reports. It discusses classifications including nondiagnostic/unsatisfactory, benign thyroid lesions, atypia of undetermined significance/follicular lesion of undetermined significance, and includes examples of diagnostic criteria, imaging features and recommended reporting language for each classification. Thyroid FNA is presented as an accurate and cost-effective initial test for evaluating thyroid nodules that can help determine if surgery is needed.
Liquid biopsy quality control – the importance of plasma quality, sample prep...Thermo Fisher Scientific
Liquid biopsy is emerging as a non-invasive companion to traditional solid tumor biopsies. As next generation sequencing (NGS) of circulating cell-free nucleic acids (cfNA = cfDNA and cfRNA) becomes common, it’s important to understand the impact of sample preparation on quality, specificity, and sensitivity of liquid biopsy tests. Plasma samples are often limited, and may have undesirable characteristics such as lipemia or hemolysis that contribute unwanted genomic DNA (gDNA) to the sample. Low cfDNA concentration can also limit the amount available for NGS library prep. In this study, we explore the effects of suboptimal plasma and low library input on liquid biopsy NGS, and discuss various techniques for in-process quality control of cfNA samples isolated from plasma
The document discusses the development and benefits of the Milan System for Reporting Salivary Gland Cytopathology. It aims to standardize terminology for salivary gland FNA reports which previously lacked uniformity. The system categorizes specimens as non-diagnostic, non-neoplastic, atypia of undetermined significance, neoplastic (benign or uncertain malignant potential), suspicious for malignancy, or malignant. It is intended to improve communication between pathologists and clinicians, enhance patient care, and facilitate research by allowing standardized data collection across institutions. While validation is ongoing, the system provides a practical framework for uniform reporting of salivary gland cytology.
1) This study analyzed the biliary anatomy of the caudate lobe in 500 living liver donors through intraoperative cholangiography.
2) It identified an average of 2.98 caudate ducts per donor and classified duct drainage patterns, finding left-to-right crossover ducts in 61.37% of cases.
3) Understanding caudate lobe anatomy through this classification aims to minimize biliary complications in both donors and recipients by guiding surgical technique and leak management.
Cervical cytology was introduced by George
Papanicolaou into clinical practice in 1940. In 1945,
the Papanicolaou smear received the endorsement of
the American cancer society as an effective method
for the prevention of cervical cancer .
www.drvikramsaraswat.co.in
www.drsaraswatpathlabs.com
Atlas on bethesda system for reporting cervical cytologyAshish Jawarkar
This is an atlas with more nearly 100 images, authentic taken from NCI web atlas. Useful to understand and report pap smears. The subject has been presented in a way which will help students reproduce in exams.
Accuracy and reliability_of_tzanck_test_compared_t (1)Eva Yustiana
This document compares the accuracy of the Tzanck test to histopathology for diagnosing basal cell carcinoma (BCC). The Tzanck test is a simple cytological examination that is faster and cheaper than histopathology, the gold standard. The study examined 26 patients clinically diagnosed with BCC using both tests. Histopathology confirmed BCC in 23 cases. The Tzanck test correctly identified BCC in 12 cases and ruled out the 3 non-BCC cases, but missed diagnosing BCC in 11 cases. The Tzanck test had a sensitivity of 52.2% and specificity of 100% compared to histopathology. While the Tzanck test can rapidly identify some BCC, it has
This document outlines an educational presentation on interpreting atypical squamous cells of undetermined significance (ASC-H) cervical cytology samples and related diagnoses. The presentation aims to describe the cytomorphological spectrum of ASC-H, define low-grade squamous intraepithelial lesion-cannot exclude high-grade (LSIL-H), discuss differential diagnoses of hyperchromatic crowded groups, and apply ancillary tests. It is divided into three sections, with the first focusing on morphological evaluation, the second on study cases using two-color immunochemistry, and the third on quality improvement, HPV testing, and medicolegal aspects.
The document presents five case studies demonstrating the utility of cytomorphology in interpreting atypical squamous cells of undetermined significance-cannot exclude high-grade squamous intraepithelial lesion (ASC-H) pap tests. Each case study describes the cytological features of the ASC-H pap test and the follow-up HPV testing results. The document also discusses the application of two-color immunohistochemistry using p16 and Ki-67 markers to help evaluate abnormal cervical cells.
CytoJournal- Open Access & CMAS on EUS FNA of Pancreasvshidham
This presentation discusses about Open Access Publishing and evolution of CMAS on EUS FNA of pancreatic lesions. A few case studies are also discussed.
Handout for Round table #19 at American Society of Cytopathology (Nov 9, 2013...vshidham
This document outlines Vinod Shidham's presentation on cell blocks. It begins with an introduction to cell blocks and their role in cytopathological evaluation and patient management. It describes various methods for preparing cell blocks from different specimen types and highlights critical issues to consider, such as specimen cellularity and intended ancillary tests. The document discusses aligning cells along the cutting surface and depth of section cutting to improve immunophenotyping on cell blocks. It presents the subtractive coordinate immunoreactivity pattern approach for effusion immunocytochemistry and concludes with several case studies.
Round table #19 at American Society of Cytopathology (Nov 5, 2012, Las Vegas,...vshidham
This document discusses cell blocks for molecular tests and immunocytochemistry applications. It begins with an overview of cell blocks and their role in bridging cytology and histopathology. It then discusses various methods for preparing cell blocks from different specimen types, noting critical issues to consider like specimen cellularity and anticipated ancillary tests. The document also covers immunophenotyping using cell blocks and challenges like antigen loss. It presents an approach called SCIP for immunocytochemistry of effusions. Finally, it indicates there will be a discussion of study cases using cell blocks.
Presentation at Chittaranjan Seva Sadan, Kolkata where Dr Dasgupta was invited as faculty in the CME organized by Medical Education and research Committee, Bengal Obstetrics and Gynaecological Society
Cell blocks in cytol (iac wrkshp i) 5 11-10vshidham
The document summarizes a workshop on cell blocks in cytopathology. It discusses the role of cell blocks in evaluating cytopathology specimens and managing patient care. It outlines different methods for preparing cell blocks and key issues to consider for each specimen type. Immunophenotyping using cell blocks and the SCIP approach are also summarized. Several case studies are presented to demonstrate cell block applications.
Recent Advances in Pathologic Evaluation of Melanoma Sentinel Lymph Nodes. Sl...vshidham
The document discusses recent advances in the pathologic evaluation of melanoma sentinel lymph nodes. It covers topics such as appropriate immunomarkers for detecting micrometastases, the number of tissue sections that should be examined, and methods for intraoperative evaluation including immunostaining of imprint smears. The 'MCW melanoma cocktail' containing MART-1, Melan-A and tyrosinase allows detection of micrometastases and can be used to rapidly evaluate imprint smears intraoperatively in 17 minutes. Examining additional tissue sections and levels improves diagnostic accuracy for identifying melanoma metastases.
Cell blocks provide tissue fragments from FNA specimens that are processed into paraffin blocks. This allows examination of histological structure and use of ancillary tests like immunohistochemistry. Cell blocks increase diagnostic sensitivity and specificity compared to cytology alone. They require minimal effort and preserve tissue for second opinions without losing the original smears. The document discusses FNAC and cell block techniques, materials used, advantages like increased cellularity and diagnostic yield, and importance of clinical information for optimal diagnosis.
04 Presentations IV VS (8MB)- (3-28-08) .ppsvshidham
Part IV of Four part symposium: “Diagnostic Cytopathology of Serous Effusion” on April 19, 2007 at Neenah, WI, USA
(2008 Wisconsin Society of Cytology, 40th Anniversary)
This course in "Virology and Mycology" (701) is designed to provide a comprehensive understanding of the medically important fungi and viruses. The content includes an introduction, general characteristics, life cycle, laboratory diagnosis, and the various techniques used in the identification and study of these microbes. This course will also equip students with practical skills, from preparing culture media to processing clinical samples for diagnosis.
Created by: Mr. Attuluri Vamsi Kumar, Assistant Professor, Department of MLT, UIAHS, Chandigarh University, Mohali, Punjab. For more details website: https://www.mltmaster.com
Global liquid biopsy market opportunity & technology outlook 2020Rajesh Sarma
Global Liquid Biopsy Market Opportunity & Technology Outlook 2020 provides an overview of the liquid biopsy market. Liquid biopsy is a non-invasive method that analyzes blood or other bodily fluids to detect biomarkers associated with cancer and other diseases. It has three main techniques - analysis of circulating tumor cells, cell-free tumor DNA, and exosomes. Next generation sequencing is also used to analyze genomic and proteomic sequences from tumor cells captured in liquid biopsies. The report examines the clinical uses and advantages of liquid biopsy compared to traditional tissue biopsy for various cancer types and other indications. It also discusses market drivers and trends, as well as challenges, for the growing liquid biopsy industry.
This course in Haematology equips students with a comprehensive understanding of blood, its cellular components, laboratory safety measures, hematological studies, and quality assurance in the field of Haematology. The course includes a theoretical exploration of various aspects of Haematology, as well as practical experiments to provide hands-on experience.
Created by: Mr. Attuluri Vamsi Kumar, Assistant Professor, Department of MLT, UIAHS, Chandigarh University, Mohali, Punjab. For more details website: https://www.mltmaster.com
Join your peers and colleagues in San Francisco to gain insight and perspective on why molecular liquid biopsies have the potential to become a fulcrum in the future of precision medicine.
Dr. Vinod Shidham is a recognized leader in cytopathology and surgical pathology. He is currently a Professor of Pathology and Vice Chair at Wayne State University School of Medicine. His areas of expertise include cytopathology, surgical pathology, and he has invented and developed novel technologies and methods in these fields. He has extensive publications, serves in leadership roles for professional organizations, and is the editor-in-chief of the open access journal CytoJournal.
This course aims to provide students with an in-depth understanding of blood banking, including the concepts of blood grouping, compatibility testing for transfusion, and the management of blood resources. It also delves into the fundamental principles of genetics, focusing on inheritance patterns, chromosomal basis of inheritance, and the role of DNA and RNA in protein synthesis. Through practical exercises, students will gain hands-on experience on various techniques used in blood banking and genetics.
Created by: Mr. Attuluri Vamsi Kumar, Assistant Professor, Department of MLT, UIAHS, Chandigarh University, Mohali, Punjab. For more details website: https://www.mltmaster.com
The Role Bacteria Biofilm Have in Identifying, Classifying and Defining UTI in Laboratory and Clinical Screenings of NB Patients That Use CIC in Clinical Settings
This document outlines the challenges involved in collecting, processing, storing, and ensuring quality control of biological samples for molecular epidemiology studies. It discusses issues related to sample collection such as timing, stability, and adherence to protocols. Sample processing challenges include time sensitivity, sterility, and record keeping. Sample banking must address issues of physical space, labeling, inventory management, and deterioration over time. Quality control involves following standardized operating procedures and maintaining documentation and traceability of all samples. Future advances may include nanobarcoding and fully automated cryobanking systems. Overall, proper handling of biological samples is crucial for protecting data quality and validity.
This guide provides an overview of pleural fluid analysis to help healthcare professionals better diagnose pleural diseases. It discusses the importance of proper pleural fluid collection techniques and handling procedures. It also examines how interpreting the fluid's chemistry, cellular components, and microbiological investigations can provide insights into conditions like infections, malignancies, or inflammation. Differentiating between transudate and exudate fluids aids in determining the underlying cause of pleural effusion. Imaging studies also assist in the diagnosis by visualizing abnormalities in the pleural space. Real-life case studies demonstrate how accurately applying these diagnostic insights improves treatment decisions and patient outcomes.
01 CellBlockistry- IAC 2019 (May 6-Monday 16- 1500 to 1800)vshidham1
This document discusses cell block preparation techniques. It begins by introducing Vinod Shidham and providing his credentials. It then lists the objectives of discussing cell block preparation methods. Various methods of cell block preparation are described, including using a clot, HistoGel, gelatin embedding, agar embedding, plasma-thrombin, and collodion bag methods. Factors that influence which technique to use are outlined. The importance of cell blocks for diagnostic and research applications is highlighted.
This Advanced Haematology course focuses on various anemia types, Leukemia, hematological disorders, chromosomal studies related to these disorders, and the use of radioisotopes in haematology. The course also addresses the importance of safety measures regarding radiation hazards and the disposal of radioactive materials.
Created by: Mr. Attuluri Vamsi Kumar, Assistant Professor, Department of MLT, UIAHS, Chandigarh University, Mohali, Punjab. For more details website: https://www.mltmaster.com
This document discusses the terminology used for preinvasive cervical lesions. It begins with definitions of dysplasia and provides a historical review of terminology, including carcinoma in situ (CIS) and cervical intraepithelial neoplasia (CIN). It then describes the 2001 Bethesda System for cervical cytology reporting and the Lower Anogenital Squamous Terminology (LAST) project. The LAST project aimed to create a unified histopathological nomenclature for HPV-associated preinvasive lesions across anatomical sites, recommending a two-tiered system of low-grade squamous intraepithelial lesions (LSIL) and high-grade squamous intraepithelial lesions (HSIL).
20_Basics of Haematological diseases.pdfVamsi kumar
This syllabus covers the fundamental understanding of Hematological diseases. It's divided into three main units: the first unit delves into Anemia and Morphologic Alterations in Neutrophils, the second unit covers Bleeding Disorders and Thrombosis, and the third unit is about Monitoring of Anticoagulants with emphasis on Oral Anticoagulants and Heparin. It also includes practical experiments for each unit.
Created by: Mr. Attuluri Vamsi Kumar, Assistant Professor, Department of MLT, UIAHS, Chandigarh University, Mohali, Punjab. For more details website: https://www.mltmaster.com
This syllabus for "Applied Haematology-I" aims to equip students with comprehensive knowledge and practical skills concerning the essential aspects of haematology, including blood component estimation, staining techniques, understanding physiological variations, body fluid examination, and coagulation studies. It is designed to provide students with a deep understanding of theoretical concepts and their practical applications, which are crucial for a career in medical laboratory technology.
Created by: Mr. Attuluri Vamsi Kumar, Assistant Professor, Department of MLT, UIAHS, Chandigarh University, Mohali, Punjab. For more details website: https://www.mltmaster.com
The syllabus focuses on providing students with a comprehensive understanding of the hematology laboratory, including its role in healthcare, essential techniques used in hematological testing, advanced hematology techniques for diagnosing disorders, and quality control procedures and troubleshooting in the lab.
Created by: Mr. Attuluri Vamsi Kumar, Assistant Professor, Department of MLT, UIAHS, Chandigarh University, Mohali, Punjab. For more details website: https://www.mltmaster.com
Banff vca consensus statemet l cendales sent to kimKim Solez ,
The document summarizes discussions from a workshop on vascularized composite allotransplantation (VCA) pathology. It addresses several unanswered questions in the field and proposes updates to the Banff VCA classification system. Key points discussed include:
- Reviewing the specificity and significance of isolated dyskeratotic/apoptotic cells in different skin structures for grading rejection.
- Studying the roles of C4d staining, antibody functions, and chronic changes like vasculopathy.
- Addressing questions from an AST working group on expanding grading criteria, differences between hand and face transplants, optimal biopsy size and location.
- Proposing a standardized biopsy collection form and digital library to facilitate
The Third International Consensus Definitions for Sepsis and Septic Shock (Se...Willian Rojas
The task force updated the definitions of sepsis and septic shock based on advances in understanding the pathobiology of sepsis. Sepsis is now defined as life-threatening organ dysfunction caused by a dysregulated host response to infection. Organ dysfunction can be identified clinically as a Sequential Organ Failure Assessment (SOFA) score of 2 points or greater. Septic shock is defined as a subset of sepsis with profound circulatory and metabolic abnormalities associated with higher mortality than sepsis alone, and can be identified by vasopressor need and hyperlactatemia in the absence of hypovolemia. The task force also proposed new clinical criteria called quickSOFA to help rapidly identify patients with suspected infection who are at higher risk of poor outcomes
The document summarizes the process undertaken by an international task force to update the definitions of sepsis and septic shock. The task force concluded that previous definitions had limitations and needed reexamination based on advances in understanding the pathobiology of sepsis. The task force developed new definitions of sepsis as a life-threatening organ dysfunction caused by a dysregulated host response to infection. Septic shock was defined as a subset of sepsis with profound circulatory and metabolic abnormalities associated with high mortality. The task force also established new clinical criteria for identifying sepsis and septic shock in patients aimed at facilitating earlier recognition and management.
Similar to 00 Initial Pages A Through C (10 26 07) Vapm (20)
KYC Compliance: A Cornerstone of Global Crypto Regulatory FrameworksAny kyc Account
This presentation explores the pivotal role of KYC compliance in shaping and enforcing global regulations within the dynamic landscape of cryptocurrencies. Dive into the intricate connection between KYC practices and the evolving legal frameworks governing the crypto industry.
13 Jun 24 ILC Retirement Income Summit - slides.pptxILC- UK
ILC's Retirement Income Summit was hosted by M&G and supported by Canada Life. The event brought together key policymakers, influencers and experts to help identify policy priorities for the next Government and ensure more of us have access to a decent income in retirement.
Contributors included:
Jo Blanden, Professor in Economics, University of Surrey
Clive Bolton, CEO, Life Insurance M&G Plc
Jim Boyd, CEO, Equity Release Council
Molly Broome, Economist, Resolution Foundation
Nida Broughton, Co-Director of Economic Policy, Behavioural Insights Team
Jonathan Cribb, Associate Director and Head of Retirement, Savings, and Ageing, Institute for Fiscal Studies
Joanna Elson CBE, Chief Executive Officer, Independent Age
Tom Evans, Managing Director of Retirement, Canada Life
Steve Groves, Chair, Key Retirement Group
Tish Hanifan, Founder and Joint Chair of the Society of Later life Advisers
Sue Lewis, ILC Trustee
Siobhan Lough, Senior Consultant, Hymans Robertson
Mick McAteer, Co-Director, The Financial Inclusion Centre
Stuart McDonald MBE, Head of Longevity and Democratic Insights, LCP
Anusha Mittal, Managing Director, Individual Life and Pensions, M&G Life
Shelley Morris, Senior Project Manager, Living Pension, Living Wage Foundation
Sarah O'Grady, Journalist
Will Sherlock, Head of External Relations, M&G Plc
Daniela Silcock, Head of Policy Research, Pensions Policy Institute
David Sinclair, Chief Executive, ILC
Jordi Skilbeck, Senior Policy Advisor, Pensions and Lifetime Savings Association
Rt Hon Sir Stephen Timms, former Chair, Work & Pensions Committee
Nigel Waterson, ILC Trustee
Jackie Wells, Strategy and Policy Consultant, ILC Strategic Advisory Board
An accounting information system (AIS) refers to tools and systems designed for the collection and display of accounting information so accountants and executives can make informed decisions.
Navigating Your Financial Future: Comprehensive Planning with Mike Baumannmikebaumannfinancial
Learn how financial planner Mike Baumann helps individuals and families articulate their financial aspirations and develop tailored plans. This presentation delves into budgeting, investment strategies, retirement planning, tax optimization, and the importance of ongoing plan adjustments.
Calculation of compliance cost: Veterinary and sanitary control of aquatic bi...Alexander Belyaev
Calculation of compliance cost in the fishing industry of Russia after extended SCM model (Veterinary and sanitary control of aquatic biological resources (ABR) - Preparation of documents, passing expertise)
How to Invest in Cryptocurrency for Beginners: A Complete GuideDaniel
Cryptocurrency is digital money that operates independently of a central authority, utilizing cryptography for security. Unlike traditional currencies issued by governments (fiat currencies), cryptocurrencies are decentralized and typically operate on a technology called blockchain. Each cryptocurrency transaction is recorded on a public ledger, ensuring transparency and security.
Cryptocurrencies can be used for various purposes, including online purchases, investment opportunities, and as a means of transferring value globally without the need for intermediaries like banks.
“Amidst Tempered Optimism” Main economic trends in May 2024 based on the results of the New Monthly Enterprises Survey, #NRES
On 12 June 2024 the Institute for Economic Research and Policy Consulting (IER) held an online event “Economic Trends from a Business Perspective (May 2024)”.
During the event, the results of the 25-th monthly survey of business executives “Ukrainian Business during the war”, which was conducted in May 2024, were presented.
The field stage of the 25-th wave lasted from May 20 to May 31, 2024. In May, 532 companies were surveyed.
The enterprise managers compared the work results in May 2024 with April, assessed the indicators at the time of the survey (May 2024), and gave forecasts for the next two, three, or six months, depending on the question. In certain issues (where indicated), the work results were compared with the pre-war period (before February 24, 2022).
✅ More survey results in the presentation.
✅ Video presentation: https://youtu.be/4ZvsSKd1MzE
1. Diagnostic Cytopathology of Serous Effusions Vinod B. Shidham , MD, FRCPath, FIAC Professor Executive editor & co-editor-in-chief, CytoJournal (www.cytojournal.com) Director- Cytopathology Fellowship Training Program & FNA Service Department of Pathology Medical College of Wisconsin 9200 W Wisconsin Av, Milwaukee, WI 53226, USA [email_address] A Vidarbha Association of Pathologists & Microbiologists (VAPM) Four session symposium Dec 9, 2007; Nagpur, MS, India Diagnostic Cytopathology of Serous Effusions
2. Note: For the benefit of participants, the handouts provided for this workshop have more information with additional reference material than that presented at the workshop. The content of the handout and that of presentation, may vary depending on time factor. B Objectives 1. Cytomorphology of benign and malignant effusions 2. Approach to immunocytochemical evaluation 3. Emphasize diagnostic cytopathology pitfalls Disclaimer and acknowledgment Most of the images and tables are based on chapters in- Shidham VB and Atkinson BF . Cytopathologic Diagnosis of Serous Fluids First edition, 2007. Elsevier ( W. B. Saunders Company) Elsevier link related to the book- http://www.us.elsevierhealth.com/product.jsp?isbn=9781416001454#description Diagnostic Cytopathology of Serous Effusions
3. Outline C Session I : Anatomy, histology, cytology, and effusions Collection, transportation, and processing of effusion fluids Factors leading to potential diagnostic pitfalls Approach to diagnostic cytopathology of effusions The panorama of different faces of mesothelial cells Session II : Benign conditions Mesothelioma Metastatic carcinoma Metastatic sarcoma and melanoma Hematolymphoid disorders (Lymphomas and leukemias) Session III : Immunocytochemistry of effusion fluids: SCIP (Subtractive Coordinate Immunoreactivity Pattern) approach Evaluation of unknown primary sites of origin- Where do they come from? Flow cytometry, mol tech, and other special techniques Session IV : Diagnostic cytopathology of peritoneal washings Diagnostic pitfalls in cytopathology of serous cavity fluids Study cases Diagnostic Cytopathology of Serous Effusions