This document outlines a workshop on the diagnostic cytopathology of serous effusions, focusing on cytomorphology, immunocytochemical evaluation, and common diagnostic pitfalls. It includes details on the anatomy, histology, and various conditions related to serous effusions, as well as the application of special techniques like flow cytometry. The content serves as both a reference for participants and a basis for discussions during the symposium.

1. Diagnostic Cytopathology of Serous Effusions Vinod B. Shidham , MD, FRCPath, FIAC Professor Executive editor & co-editor-in-chief, CytoJournal (www.cytojournal.com) Director- Cytopathology Fellowship Training Program & FNA Service Department of Pathology Medical College of Wisconsin 9200 W Wisconsin Av, Milwaukee, WI 53226, USA [email_address] A Vidarbha Association of Pathologists & Microbiologists (VAPM) Four session symposium Dec 9, 2007; Nagpur, MS, India Diagnostic Cytopathology of Serous Effusions

2. Note: For the benefit of participants, the handouts provided for this workshop have more information with additional reference material than that presented at the workshop. The content of the handout and that of presentation, may vary depending on time factor. B Objectives 1. Cytomorphology of benign and malignant effusions 2. Approach to immunocytochemical evaluation 3. Emphasize diagnostic cytopathology pitfalls Disclaimer and acknowledgment Most of the images and tables are based on chapters in- Shidham VB and Atkinson BF . Cytopathologic Diagnosis of Serous Fluids First edition, 2007. Elsevier ( W. B. Saunders Company) Elsevier link related to the book- http://www.us.elsevierhealth.com/product.jsp?isbn=9781416001454#description Diagnostic Cytopathology of Serous Effusions

3. Outline C Session I : Anatomy, histology, cytology, and effusions Collection, transportation, and processing of effusion fluids Factors leading to potential diagnostic pitfalls Approach to diagnostic cytopathology of effusions The panorama of different faces of mesothelial cells Session II : Benign conditions Mesothelioma Metastatic carcinoma Metastatic sarcoma and melanoma Hematolymphoid disorders (Lymphomas and leukemias) Session III : Immunocytochemistry of effusion fluids: SCIP (Subtractive Coordinate Immunoreactivity Pattern) approach Evaluation of unknown primary sites of origin- Where do they come from? Flow cytometry, mol tech, and other special techniques Session IV : Diagnostic cytopathology of peritoneal washings Diagnostic pitfalls in cytopathology of serous cavity fluids Study cases Diagnostic Cytopathology of Serous Effusions