Transport process in bacteria. Binding protein dependent transport systems

1. BACTERIAL PLASMA MEMBRANE
PERMEASES
Transport process in bacteria.
Binding protein dependant transport
systems.
Shruthi Shree Gandhi.S,
I MSC.,Biochemistry
Sri Ramakrishna College of Arts and Science
for Women.

2. Transport process in bacteria
• The bacterial membrane adopts various
mechanism for the transport of sugars in to the
bacterial cell.
• Depending on the species and the surrounding
environment they live .

3. The transport process in bacteria
follows three mechanism.

4. Binding-protein dependent transport
systems (BPDT)
• The histidine permease transport system is one of the
example of BPDT in E.coli.
• It belongs to the ABC superfamily transporters.
• They involve in active transport of solute molecules such
as amino acids (especially uptake of histidine in
histidine permeases ),vitamins,sugars and peptides in to
the cell.

5. • The energy required for this process is provided by the
hydrolysis of ATP
• The quantity of transport protein in to the cell is
regulated by both the concentration of the nutrient in the
medium and the metabolic needs of the cell.

6. STRUCTURE AND MECHANISM

7. • Histidine is selectively uptaken through the porin in outer
membrane.
• The histidine molecule entering the periplasmic space is carried by a
carrier protein called histidine binding protein.
• The histidine binding protein binds to the histidine permease in
the plasma membrane.
• The histidine permeases comprises of two transmembrane domains
and two cytocolic ATP binding domains.forming four separate
subunits.
• The binding of the carrier protein is require for the histidine
permeases to selectively uptake the molecule.
• Transport of histidine in to the cytoplasm requiresATP.

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