Uniporter proteins transport molecules like amino acids and sugars across cell membranes. GLUT1 is a specific uniporter protein that transports glucose. It is encoded by the SLC2A1 gene and belongs to the major facilitator super family. GLUT1 is expressed in fetal tissues, erythrocytes, and the endothelial cells of the blood brain barrier where it regulates glucose uptake. It undergoes conformational changes to alternately expose its glucose binding site inside and outside the cell to transport glucose across the membrane. GLUT1 plays important roles in supplying glucose to the brain and developing fetus.

1. Uniporter Catalyzed Transport
GLUT 1 TRANSPORTERS
Shruthi Shree Gandhi.S
I Msc.Biochemistry
Sri Ramakrishna College Of Arts and Science for
women

2. UNIPORTER CATALYSED TRANSPORTER
The plasma membrane of various tissues
contains uniporter proteins that can transport
amino acids, sugars and other small
molecules.
Uniporter carrier proteins catalyzes
facilitated diffusion that is they transport
molecules against their concentration
gradient.

3. Uniporter is a type of protein that allows only single
or one type of molecule to pass through them .
They posses specific binding site for molecules and
can undergo conformational changes upon binding with
molecules.

4. GLUT 1 TRANSPORTERS
• GLUT TRANSPORTERS
• These are carrier proteins .There are
various types of GLUT transporters present
in human tissues that transport specific
type of molecules depending on their
location.
• The GLUT transporters are coded by
SLC2A gene
• Example:
https://en.wikipedia.org/wiki/Glucose_transporter

5. GLUT 1 TRANSPORTERS
• Glut 1 transporter is a carrier protein
encoded by SLC2A1 gene and belongs
to the members of major facilitator
super family (MFS)membrane proteins.
• It is expressed in fetal tissues, highly
in erythrocytes and endothelial cells
of blood brain barrier. they regulate
the uptake of glucose in the cells and
tissues they preset.

6. • Glut1 is made up of 492 amino
acids,
• That is 12 membrane spanning
alpha helices each containing of 20
different amino acids.

7. MECHANISM
GLUT 1 protein alternates in to two different conformational
changes which facilitates the transport of glucose across the
membrane.

8. • When the binding site of the GLUT 1
transporter is facing the exterior of the cell
(T1 STATE) ,the substrate molecule (glucose)
binds to the binding site of the GLUT 1 protein.
• The GLUT1 protein undergoes conformational
change and the binding site now faces the
cytosol of the cell(T2 STATE).
• Where the glucose molecule is released into
the cytosol of the cell. the opposite can happen
when there is less glucose outside the cell.
• Inhibitors like fastenin,bay 76 inhibits glucose
uptake

9. FUNCTIONS
• GLUT 1 proteins are highly expressed in
erythrocytes plasma membrane for carrying
the glucose in the blood.
• it is present in endothelial cells of blood brain
barrier as it plays important role in uptake of
glucose in cerebrum which is essential for
brain as it is dependant on glucose as fuel
source.
• it is present in fetal tissues and plays an
important role in the development of pre-
implantation of embryo.

10. THANK YOU