Various human diseases have oxidative stress as one of their component. Many herbs have been reported to exhibit properties that combat oxidative stress through their active constituents such as flavonoids, tannins, phenolic compounds etc. Different Plants of Dillenicea family has been shown in in vitro experiments to be endowed with antioxidant activity. Therefore this study was carried out to evaluate Dillenicea family for its antioxidant activity.

1. A Comprehensive Review Study
Of Antioxidant Potential of
Dillenicea Family
Presented by :
Exam Roll No:M-10203
Registration No: 0407
Session : July 2015
University of Science and Technology Chittagong (USTC)
Department of Pharmacy

2. Introduction
Various human diseases have oxidative stress as one of their
component. Many herbs have been reported to exhibit properties
that combat oxidative stress through their active constituents such as
flavonoids, tannins, phenolic compounds etc. Different Plants of
Dillenicea family has been shown in in vitro experiments to be
endowed with antioxidant activity. Therefore this study was carried
out to evaluate Dillenicea family for its antioxidant activity.

3.  A lot of modern medicines have been discovered as a result of the scientific follow-
up of the traditional herbal preparations.
Still there is no cure for the treatment of diseases caused by free radicals like
Alzheimer’s disease, cancer etc.
Investigation of the bioactive principles of antioxidant properties with less side effects
is very essential to make the drug affordable to local people with low price.

4. Plants List of Dillenicea
family
1Pentagyna Roxb
2 Tetracera Sarmentosa
3 Dillenia indica L.
Ref: http://ethnobotanybd.com/index.php?action=Taxonomy&key=sci

5. PLANT PROFILE REVIEW
Dillinia Indica L.
Ref: http://ethnobotanybd.com/index.php?action=Taxonomy&key=sci

6. Pentagyna Roxb.
Ref: http://ethnobotanybd.com/index.php?action=Taxonomy&key=sci

7. Determination of antioxidant and analgesic activities of
Different Plants of Dillenicea family
Antioxidant Test
a)DPPH radical scavenging
b)Reducing Power
c)Total Phenol and
d) Total Flavonoid content
AIM AND OBJECTIVES

8. MATERIALS AND METHODS
Antioxidant properties
Antioxidant activity determined by DPPH,
reducing power and total antioxidant. Secondary
metabolite content determined by
Spectrophotometric process.
Reagents :
1. Potassium ferricyanide [K3Fe(CN)6]
2. Trichloro Acetic acid
3. Ferric Chloride (FeCl3)
4. Phosphate buffer [K2HPO4 + KH2PO4]
5. Ascorbic acid (Analytical or Reagent
grade)
6. Water bath
7. Centrifuge machine
8. Pipette (1-10 ml)
9. DPPH
10. UV spectrophotometer
Ref :Clarke, Garry, et al. "High correlation of 2, 2-diphenyl-1-picrylhydrazyl (DPPH)
radical scavenging, ferric reducing activity potential and total phenolics content
indicates redundancy in use of all three assays to screen for antioxidant activity of
extracts of plants from the malaysian rainforest." Antioxidants 2.1 (2013): 1-10.

9. DPPH (1,1-Diphenyl-2- Picrylhydrazyl ) RADICAL SCAVENGING ACTIVITY
N-N(Ph)2
NO2
NO2 NO2
+ A-H
H-N-N(Ph)2
NO2
NO2 NO2
+ A
DPPH
.
DPPH
.
The radical scavenging potential of the test samples are determined by measuring
the decrease in absorbance due to the reduced form of DPPH. The absorbance
measured at 517nm.
The percentage (%) of Scavenging activity (scv) was calculated from the following equation:
% scv = {( A0 – A1 )/A0} X 100
Where,
A0 is the absorbance of the control and
A1 is the absorbance of the extract/standard.
Ref :Clarke, Garry, et al. "High correlation of 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging, ferric reducing activity potential and total phenolics
content indicates redundancy in use of all three assays to screen for antioxidant activity of extracts of plants from the malaysian rainforest." Antioxidants 2.1

10. Fig. 01: % scavenging activity of ascorbic acid and methanol extract of Tetracera Sarmentosa
at different concentration
Tetracera Sarmentosa
Ref :Clarke, Garry, et al. "High correlation of 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging, ferric reducing activity potential and total phenolics
content indicates redundancy in use of all three assays to screen for antioxidant activity of extracts of plants from the malaysian rainforest." Antioxidants 2.1
(2013): 1-10.

11. Table 2: IC50 values of Tetracera Sarmentosa &
ascorbic acid (standard)
Sample/Standard IC50 (g/ml)
Tetracera Sarmentosa 26.30
Ascorbic acid 1.748
Reducing Power Capacity
Table 3: Absorbance of Tetracera Sarmentosa and ascorbic acid (Standard) at four
concentrations
Concentration
µg/ml
Reducing power of
Tetracera Sarmentosa
Reducing power of Ascorbic
Acid
125 0.294 1.000
250 0.336 1.504
500 0.458 1.598
1000 0.575 1.878

12. Ref: Liu, Jun, et al. "Free radical mediated grafting of chitosan with caffeic and ferulic acids: Structures and antioxidant activity.
" International journal of biological macromolecules 65 (2014): 97-106.

13. Ref: Carlsen, Monica H., et al. "The total antioxidant content of more than 3100 foods, beverages, spices, herbs and supplements used worldwide.“
Nutrition journal 9.1 (2010): 1.

14. Table 4:Total phenol content of T.Sarmentosa with necessary data
Ref: Liu, Jun, et al. "Free radical mediated grafting of chitosan with caffeic and ferulic acids: Structures and antioxidant activity.
" International journal of biological macromolecules 65 (2014): 97-106.

15. Antioxidant activity of Dillenia indica L &
Pentagyna Roxb
Reducing Power Capability
Concentration
µg/ml
Reducing power of
Pentagyna Roxb
Reducing power
Of Dillenia indica L.
Reducing power of
Ascorbic Acid
0 0 0 0
125 0.979 0.769 1.000
150 1.123 0.869 1.504
500 1.347 1.124 1.598
1000 1.507 1.369 1.878
Ref: Liu, Jun, et al. "Free radical mediated grafting of chitosan with caffeic and ferulic acids: Structures and antioxidant activity.
" International journal of biological macromolecules 65 (2014): 97-106.

16. Antioxidant activity of Dillenia indica L & Pentagyna
Roxb
y = 0.001x + 0.4618
R² = 0.6649
y = 0.0011x + 0.6068
R² = 0.5543
0
0.2
0.4
0.6
0.8
1
1.2
1.4
1.6
1.8
0 200 400 600 800 1000 1200
Reducingpower
Concentration ug/mL
Reducing potential of Dillenia indica L.
& Pentagyna Roxb.
Reducing power of D.I
Reducing power of D.P
Linear (Reducing power of D.I)
Linear (Reducing power of D.P)
Ref: Liu, Jun, et al. "Free radical mediated grafting of chitosan with caffeic and ferulic acids: Structures and antioxidant activity.
" International journal of biological macromolecules 65 (2014): 97-106.

17. Determination of total Phenol Content of D.I & P.R
y = 0.0039x + 0.033
R² = 0.9987
0
0.5
1
1.5
2
2.5
0 200 400 600
Absorbance
concentration
Calibration curve of gallic Acid
absorbance
Linear (absorbance)
Ref: Carlsen, Monica H., et al. "The total antioxidant content of more than 3100 foods, beverages, spices, herbs and supplements used worldwide.“
Nutrition journal 9.1 (2010): 1.

18. Sample solution
(µg/ml)
Weight of dry
extract per ml
m(gm)
Absorbance GAE conc.
C
(µg/ml)
GAE conc.
C
(mg/ml)
TPC as
GAE,
A=
(µg/ml)
Mean±
SEM
1000 0.001 1.04 335.67 0.34 167.83 167.44
1000 0.001 1.034 333.67 0.33 166.83 ±
1000 0.001 1.039 335.33 0.34 167.67 0.31
Sample solution
(µg/ml)
Weight of dry
extract per ml
m(gm)
Absorbance GAE conc.
C
(µg/ml)
GAE conc.
C
(mg/ml)
TPC as
GAE,
A=
(µg/ml)
Mean±
SEM
1000 0.001 0.851 252.09 0.27 136.33 133.01
1000 0.001 0.825 264.00 0.26 132.00 ±
1000 0.001 0.837 268.00 0.27 134.00 1.25
Data for the determination phenol content of Dillenia indica L
extract
Data for the determination phenol content of Pentagyna Roxb
extract
Ref: Carlsen, Monica H., et al. "The total antioxidant content of more than 3100 foods, beverages, spices, herbs and supplements used worldwide.“
Nutrition journal 9.1 (2010): 1.

19. CONCLUSION
Different plants of Dillenicea family showed moderate antioxidant
activity by inhibiting DPPH, and reducing power activities when
compared with standard ascorbic acid. In addition, the extracts
were found to contain a noticeable amount of total phenols and
flavonoid which play a major role in controlling oxidation. The
results of this study show that the extract can be used as an easily
accessible source of natural antioxidant. Therefore, it is suggested
that activity–guided isolation study should be performed.

20. Thank you