Anti oxidant activity of yucca gloriosa

1. _____________________
* Corresponding author:
Sribalaji college of pharmacy,
Choppadandi,
Karimnagar,
A.P. India
E-mail address: rrv.raj.veerla@gmail.com
Available Online at: www.ijrpp.com
In-Vitro antioxidant activity of the petroleum ether extract of Yucca
Gloriosa L.
*1
Rajendar Rao.V, 2
B.N.V. Bhargavi,
Sri Balaji College Of Pharmacy,
________________________________________
ABSTRACT
The present study was undertaken to evaluate the
petroleum ether extract of Yucca gloriosa L.
picryl hydrazyl) radical scavenging, and reducing power assays. The results show that the plant
extracts of Yucca gloriosa L. possess antioxidant activity when compared with standard shows
same.
Keywords: Yucca gloriosa L., DPPH,
_________________________________________________________
INTRODUCTION
Antioxidants are substances that may protect cells
from the damage caused by unstable
known as free radicals. Antioxidants interact with
and stabilize free radicals and may prevent some of
the damage-free radicals might otherwise cause.
Antioxidant acts as cell protectors. Oxygen, an
essential element for life, can create damaging
by-products during normal cellular metabolism.
Antioxidants counteract these cellular by
during normal cellular metabolism. Antioxidants
counteract these cellular by-products called free
radicals, and blind with them before they can cause
damage. If left unchecked, free radicals may cause
heart damage, cancer, cataracts, and a weak
immunes system. Antioxidants come in a variety of
forms include vitamin C, vitamin E, Carotenoids
[1,2].
Yucca gloriosa L. commonly known as Spanish
Dagger and Family- Agavaceae. It is a stems less or
Available Online at: www.ijrpp.com Print ISSN: 2278 - 2648
Online ISSN: 2278 - 2656
(Research article)
Vitro antioxidant activity of the petroleum ether extract of Yucca
B.N.V. Bhargavi, 3
Soujanya.G
Sri Balaji College Of Pharmacy, Choppadandi, Karimnagar, A.P, India.
_____________________________________________________________________
The present study was undertaken to evaluate the in vitro antioxidant activities of whole plant of
gloriosa L. The plant extract was tested for DPPH (2, 2
picryl hydrazyl) radical scavenging, and reducing power assays. The results show that the plant
possess antioxidant activity when compared with standard shows
DPPH, Reducing Power Assay.
____________________________________________________________________
Antioxidants are substances that may protect cells
from the damage caused by unstable molecules
known as free radicals. Antioxidants interact with
and stabilize free radicals and may prevent some of
might otherwise cause.
Antioxidant acts as cell protectors. Oxygen, an
essential element for life, can create damaging by,
products during normal cellular metabolism.
Antioxidants counteract these cellular by-products
during normal cellular metabolism. Antioxidants
products called free
radicals, and blind with them before they can cause
age. If left unchecked, free radicals may cause
heart damage, cancer, cataracts, and a weak
immunes system. Antioxidants come in a variety of
forms include vitamin C, vitamin E, Carotenoids
commonly known as Spanish
Agavaceae. It is a stems less or
rising of stature of small trees and trunk short. This
plant leave is 2-3 feet long, 2 in wide, long pointed,
often tooth margin, mostly in rosettes at the surface
of ground or ends of the trunk. Flowers have
cups or saucer shaped, hanging, greenish white to
reddish, fragrant, born mostly in erect panicles that
usually overtop the leaves. The whole plant of
gloriosa L. is used for ulcer, asthma and bronchitis
[3]. From the source of literature docume
relevant traditional approaches on plant drugs, the
present investigation was carried out to investigate
the invitro anti-oxidant activity of the Petroleum
Ether Extract of Yucca gloriosa L. whole plant
is being reported here.
MATERIALS AND METHODS
Plant material
The whole plant of Yucca gloriosa L.
from Tirumala hills, Tirupati, Andhra Pradesh. India.
International Journal of
Research in Pharmacology and
Pharmacotherapeutics
10
(Research article)
Vitro antioxidant activity of the petroleum ether extract of Yucca
_____________________________
antioxidant activities of whole plant of
The plant extract was tested for DPPH (2, 2-diphenyl, 2-
picryl hydrazyl) radical scavenging, and reducing power assays. The results show that the plant
possess antioxidant activity when compared with standard shows the
___________
rising of stature of small trees and trunk short. This
3 feet long, 2 in wide, long pointed,
often tooth margin, mostly in rosettes at the surface
of ground or ends of the trunk. Flowers have many
cups or saucer shaped, hanging, greenish white to
reddish, fragrant, born mostly in erect panicles that
The whole plant of Yucca
is used for ulcer, asthma and bronchitis
[3]. From the source of literature documentation and
relevant traditional approaches on plant drugs, the
present investigation was carried out to investigate
oxidant activity of the Petroleum
whole plant (PYG)
AND METHODS
Yucca gloriosa L. was collected
from Tirumala hills, Tirupati, Andhra Pradesh. India.
International Journal of
Research in Pharmacology and
Pharmacotherapeutics

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It was identified and authenticated by Prof. Madhava
Chetty, K., Taxonomist, S.V. University, Tirupati,
Andhra Pradesh, India. A voucher specimen has
been kept in our laboratory for future reference.
Preparation of plant extract
The collected whole plant was dried at room
temperature, pulverized by a mechanical grinder,
sieved through 40mesh. About 100g of powdered
materials were extracted with petroleum ether (60°-
80°C) using soxhlet apparatus. The extraction was
carried out until the extractive becomes colourless.
The extract is then concentrated and dried under
reduced pressure. The solvent free semisolid mass
thus obtained is dissolved in tween 80 and used for
the experiment. The percentage yield of prepared
extracts was around 10.5%w/w.
ANTIOXIDANT ACTIVITY
DPPH method
This is the most widely reported method for
screening of anti-oxidant activity of many plant
drugs. DPPH assay method is based on the reduction
of methanol solution of Coloured free radical DPPH
by free radical scavenger. The procedure involves
measurement of decrease in absorbance of DPPH at
its absorption maxima of 516 nm, which is
proportional to concentration of free radical
scavenger added to DPPH reagent solution. The
activity is expressed as effective concentration EC50
.
The super oxide radical can also be detected by
oxidation of hydroxylamine, yielding nitrite, which is
measured colorimetric reaction.
Reducing power method
This method is based on the principle of increase in
the absorbance of the reaction mixture. Increase in
the absorbance indicates the increase in the anti-
oxidant activity. In this method, anti-oxidant
compound forms a coloured complex with
potassium ferric cyanide, trichloro acetic acid and
ferric chloride, which are measured at 700 nm.
Increase in the absorbance of the reaction mixture
indicates the reducing the power of the samples.
The following methods were used to evaluate
antioxidant activity:
DPPH Radical Scavenging Test
1. The free radical scavenging activity of the petroleum
ether extracts of Yucca gloriosa L. (PEYG) was
determined by using 2, 2 Diphenyl-l-picryl hydrazyl
radical (DPPH) using UV-Spectrometry [4] at 517 nm.
2. The DPPH solution was prepared in 95% methanol.
3. The PEYG was mixed with 95% methanol to prepare
the stock solution (10mg/100ml or 100µg/ml). From
the stock solution 2ml, 4ml, 6ml, 8ml and 10ml of
this solution were taken in five test tubes and by
serial solution with same solvent were made the final
volume of each test tube up to 10 ml whose
concentration was then 20 µg/ml, 40µg/ml, 60
µg/ml, 80 µg/ml and 100 µg/ml respectively.
4. Freshly prepared DPPH solution (0.004% w/v) was
added in each of their test tubes. Containing PEYG
(20 µg/ml, 40µg/ml, 60 µg/ml, 80 µg/ml and 100
µg/ml) and after 10 min, the absorbance was taken
at 517nm, using a spectrophotometer (Shimadzu UV-
1700, UV-visible spectrophotometer).
5. Ascorbic acid was used as a reference standard. It is
dissolved in distilled water to make the stock
solution with the same concentration of PEYG
control sample was prepared without extract and
reference ascorbic acid. 95% methanol was used as
blank % scavenging of the DPPH free radical was
measured using following equation.
%DPPH radical -
Scavenging =
Absorbance control-Absorbance of test sample)
(Asorbance of control)
× 100
Reducing Power Method
1. The assay of reducing power method [5, 6]] is one to
determine the antioxidant activity.
2. In this 1 ml of plant extract of PEYG solution mixed
with 2.5 ml phosphate buffer (0. 2M, pH 6.6) and 2.5
ml Potassium Ferricyanide [K3
Fe (CN6)] (10g/l), the
mixture was incubated at 500
C for 20 minutes.
3. 2. 5ml of Tri chloroacetic acid (100g/l) was added to
mixture. This was centrifuged at 3000 rpm for 10
min.
4. Finally, 2.5 ml of the supernatant solution was mixed
with 2.5 ml of distilled water and 0.5 ml FeCl3
and
absorbance measured at 700nm in UV- visible
spectrophotometer (SHIMADZU UV-1700, UV-visible
spectrophotometer).
5. Ascorbic acid was used as the standard and
phosphate buffer used as blank.

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RESULTS
Table 1. Antioxidant activity of petroleum ether extracts of Yucca gloriosa L. by DPPH
method.
S. No
Concentration
(µg/ml)
Absorbance of
ascorbic acid
Absorbance of
PEYG
% scavenging
DPPH of Ascorbic
acid
% scavenging
DPPH of PEYG
1 20µg/ml 0.142 0.128 35.15 41.55
2 40 µg/ml 0.102 0.089 53.42 59.36
3 60 µg/ml 0.086 0.067 60.73 69.40
4 80 µg/ml 0.058 0.039 76.51 82.19
5 100 µg/ml 0.032 0.011 85.38 94.97
DPPH radical scavenging activity of petroleum ether extracts of Yucca gloriosa L. (PEYG) added to the
methanol solution of DPPH, and radical scavenging activity was measured as 517 nm as compared to standard
ascorbic acid. Values are the average of triplicate experiments.
Table 2. Antioxidant activity of petroleum ether extracts of Yucca gloriosa L. by reducing
power method.
S. No Concentration
(µg/ml)
Absorbance of ascorbic acid Absorbance of PEYG
1 0.1 0.16 0.11
2 0.2 0.22 0.20
3 0.3 0.31 0.28
4 0.4 0.39 0.35
5 0.5 0.48 0.41
Reducing power of petroleum ether extract of Yucca gloriosa L. (PEYG) of as compared to Ascorbic acid. Values are
the average of triplicate experiments.
DISCUSSION AND CONCLUSION
The presence study establishes the antioxidant
activity of PEYG by two models i.e by comparing the
% of scavenging DPPH (DPPH method) and
determining the absorbance (Reducing power
method) of PEYG with the standard (Ascorbic acid) at
different concentrations of drug. The extract elicited
a dose-dependent antioxidant activity when
compared to standard. Hence invitro tests are
conducted to determine the antioxidant activity. The
tests conducted are DPPH method and reducing
power method. Here the standard substance taken
for the comparison is Ascorbic acid, which is
renowned antioxidant. The results show that the
plant extracts of Yucca gloriosa L. posses antioxidant
activity when compared with standard shows the
same. Further development of research may lead to
the development of new antioxidant.

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