BIO cell and molecular bio ANSWER ALL1 which of the fo.pdf

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because of a hurricane, your regular laboratory session was cancelled and the gram stain procedure was performed on cultures incubated for a longer period of time. Examination of the stain staphylococcus aureus slides revealed a great deal of color variability ranging from intese blue to shades of pink. Account for this result. Solution Staphylococcus aureus is a Gram positive, non motile bacteria. On Gram staining it will appear purple to blue color since it will retain crystal violet dye. Again, Gram negative bacteria say for example, E.coli will appear pink in color. So, here due to longer period of incubation the culture has been contaminated with Gram negative bacteria. Therefore showing color variability in Gram staining results..

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BIO: cell and molecular bio
ANSWER ALL
1 which of the follow is false?
A eukaryotic gene activator proteins stimulate transcription initiation by aiding in the assembly
of general transcription factors and RNA polymerase at the promoter
B eukaryotic gene activator proteins stimulate transcription initiation by recruiting proteins that
modify chromatin structure
C eukaryotic gene activator proteins stimulate transcription initiation by recruiting a DNA
polymerase to the promoter.
2 although all of the steps involved in expressing a gene can in principle be regulated, for most
genes the initiation of __________ is the most important point of control.
3 the general transcription factors that assemble at a eukaryotic promoter are different, depending
on the specific gene being transcribed by polymerase II.
True or False?
4 Which of the following describes the lac operon in E. coli when lactose, but not glucose, is
present in the culture medium?
A) CAP, but not the lac repressor, is bound to the lac operon's regulatory DNA, and the lac
operon is expressed.
B) CAP and the lac repressor are both bound to the lac operon's regulatory DNA, and the lac
operon is not expressed.
C) The lac repressor, but not CAP, is bound to the lac operon's regulatory DNA, and the lac
operon is not expressed.
D) Neither CAP nor the lac repressor is bound to the lac operon's regulatory DNA, and the lac

operon is not expressed.
BIO: cell and molecular bio
ANSWER ALL
1 which of the follow is false?
A eukaryotic gene activator proteins stimulate transcription initiation by aiding in the assembly
of general transcription factors and RNA polymerase at the promoter
B eukaryotic gene activator proteins stimulate transcription initiation by recruiting proteins that
modify chromatin structure
C eukaryotic gene activator proteins stimulate transcription initiation by recruiting a DNA
polymerase to the promoter.
2 although all of the steps involved in expressing a gene can in principle be regulated, for most
genes the initiation of __________ is the most important point of control.
3 the general transcription factors that assemble at a eukaryotic promoter are different, depending
on the specific gene being transcribed by polymerase II.
True or False?
4 Which of the following describes the lac operon in E. coli when lactose, but not glucose, is
present in the culture medium?
A) CAP, but not the lac repressor, is bound to the lac operon's regulatory DNA, and the lac
operon is expressed.
B) CAP and the lac repressor are both bound to the lac operon's regulatory DNA, and the lac
operon is not expressed.
C) The lac repressor, but not CAP, is bound to the lac operon's regulatory DNA, and the lac
operon is not expressed.

D) Neither CAP nor the lac repressor is bound to the lac operon's regulatory DNA, and the lac
operon is not expressed.
ANSWER ALL
1 which of the follow is false?
A eukaryotic gene activator proteins stimulate transcription initiation by aiding in the assembly
of general transcription factors and RNA polymerase at the promoter
B eukaryotic gene activator proteins stimulate transcription initiation by recruiting proteins that
modify chromatin structure
C eukaryotic gene activator proteins stimulate transcription initiation by recruiting a DNA
polymerase to the promoter.
2 although all of the steps involved in expressing a gene can in principle be regulated, for most
genes the initiation of __________ is the most important point of control.
3 the general transcription factors that assemble at a eukaryotic promoter are different, depending
on the specific gene being transcribed by polymerase II.
True or False?
4 Which of the following describes the lac operon in E. coli when lactose, but not glucose, is
present in the culture medium?
A) CAP, but not the lac repressor, is bound to the lac operon's regulatory DNA, and the lac
operon is expressed.
B) CAP and the lac repressor are both bound to the lac operon's regulatory DNA, and the lac
operon is not expressed.
C) The lac repressor, but not CAP, is bound to the lac operon's regulatory DNA, and the lac
operon is not expressed.

D) Neither CAP nor the lac repressor is bound to the lac operon's regulatory DNA, and the lac
operon is not expressed.
1 which of the follow is false?
A eukaryotic gene activator proteins stimulate transcription initiation by aiding in the assembly
of general transcription factors and RNA polymerase at the promoter
B eukaryotic gene activator proteins stimulate transcription initiation by recruiting proteins that
modify chromatin structure
C eukaryotic gene activator proteins stimulate transcription initiation by recruiting a DNA
polymerase to the promoter.
2 although all of the steps involved in expressing a gene can in principle be regulated, for most
genes the initiation of __________ is the most important point of control.
3 the general transcription factors that assemble at a eukaryotic promoter are different, depending
on the specific gene being transcribed by polymerase II.
True or False?
4 Which of the following describes the lac operon in E. coli when lactose, but not glucose, is
present in the culture medium?
A) CAP, but not the lac repressor, is bound to the lac operon's regulatory DNA, and the lac
operon is expressed.
B) CAP and the lac repressor are both bound to the lac operon's regulatory DNA, and the lac
operon is not expressed.
C) The lac repressor, but not CAP, is bound to the lac operon's regulatory DNA, and the lac
operon is not expressed.
D) Neither CAP nor the lac repressor is bound to the lac operon's regulatory DNA, and the lac
operon is not expressed.

Solution
1). B). B eukaryotic gene activator proteins stimulate transcription initiation by recruiting
proteins that modify chromatin structure
In eukaryotes, the gene activator proteins cause changes to the chromatin structure, which allow
the access of other factors necessary for transcription.
Genome, in its condensed form, it is not accessible to the transcription factors, and other factors
required for the DNA replication and recombination. This is overcome by the histone modifying
proteins modify these histone at different sites and make the genome accessible to all the
regulatory enzymes. These histone modifying enzymes act by different mechanisms such as
methylation, demethylation, acetylation, deamination, phosphorylation, etc.

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Includes definition, discovery.Enzyme induction is a process in which a molecule (e.g., a drug) induces (i.e., initiates or enhances) the expression of an enzyme. The induction of heat shock proteins in the fruit fly Drosophila melanogaster. The Lac operon is an interesting example of how gene expression can be regulated. Viruses, despite having only a few genes, possess mechanisms to regulate their gene expression, typically into an early and late phase, using collinear systems regulated by anti-terminators (lambda phage) or splicing modulators (HIV). Gal4 is a transcriptional activator that controls the expression of GAL1, GAL7, and GAL10 (all of which code for the metabolic of galactose in yeast). The GAL4/UAS system has been used in a variety of organisms across various phyla to study gene expression.

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The control of gene expression or protein synthesis is called gene regulation or it is the process of turning genes on and off Gene regulation in prokaryotes is most extensively observed at the initiation of transcription. Most genes are controlled by extracellular signals- present in medium. Repressor, a negative regulatory molecule, binds to the operator gene and interferes with the expression of genes. Activator, a positive regulatory molecule, enhances the expression of the genes. Operon : a group or cluster of structural genes under a single promoter; bacterial operons are polycistronic transcripts that are able to produce multiple proteins from one mRNA Francois Jacob and Jacques Monod in 1961 “Lac operon is an operon or a group of genes with a single promoter that encode genes for the transport and metabolism of lactose in E.coli and other bacteria.” Lac operon concept is an example of prokaryotic gene regulation.

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