1. Chapter Four The Three-Dimensional Structure of Proteins

7.  -Helix (Cont’d)

10.  -Pleated Sheet (Cont’d)

14. Schematic Diagrams of Supersecondary Structures

18. Comparison of Shapes of Fibrous and Globular Proteins

21. Forces That Stabilize Protein Structure

24. High resolution method to determine 3˚ structure of proteins (from crystal) Diffraction pattern produced by electrons scattering X-rays Series of patterns taken at different angles gives structural information Determines solution structure Structural info. Gained from determining distances between nuclei that aid in structure determination X-Ray and NMR Data

26. The Structure of Myoglobin

27. Oxygen Binding Site of Myoglobin

29. Denaturation of a Protein

30. Several ways to denature proteins • Heat • pH • Detergents • Urea • Guanadine hydrochloride Denaturation and Refolding in Ribonuclease

33. Structure of Hemoglobin

35. Oxy- and Deoxyhemoglobin

37. Predicting Protein Structure

39. Hydrophobic and Hydrophilic Interactions in Proteins