Selfing and crossing are the essential procedures in crop improvement process, and plant breeding kits helps breeder to achieve the objectives. Selfing- pollen grain of a flower pollinate the same flower. The objective of selfing is to avoid cross pollination and ensure self-pollination.

1. Dr. Indrajay R. Delvadiya
To study the plant breeder's kit's components

2. By :
Dr. Delvadiya Indrajay R.
Assistant Professor
26813
Genetics & Plant Breeding
LPU
EX- 1
Dr. Indrajay R. Delvadiya

3. Experiment 1: To study the plant
breeder's kit's components
• Selfing and crossing are the essential
procedures in crop improvement process, and
plant breeding kits helps breeder to achieve the
objectives.
• Selfing- pollen grain of a flower pollinate the
same flower.
• The objective of selfing is to avoid cross
pollination and ensure self-pollination.
Dr. Indrajay R. Delvadiya

4. Procedure of hybridisation
Choice of parents
Evaluation of parents
Emasculation
Bagging
Tagging
Pollination
Harvesting and storage of F1 seed
Dr. Indrajay R. Delvadiya

5. Rice
Dr. Indrajay R. Delvadiya

6. Dr. Indrajay R. Delvadiya

7. Dr. Indrajay R. Delvadiya

8. WASHING BOTTLE
USE : It is used for filling sterilizing agent like alcohol or spirit to sterilize the scissors,
pointers, forceps and brush during crossing work
Dr. Indrajay R. Delvadiya

9. EYE LENS OR MAGNIFYING LENS
• USE : For observing the reproductive parts to confirm that there should not be any
part of the anther left on the stigma or stigma is free from any foreign pollens.
Dr. Indrajay R. Delvadiya

10. USE : It is used for incising the floral buds and for removing
the anthers from it. e.g. Tobacco, Sesamum etc
FINE POINTED FORCEPS
Dr. Indrajay R. Delvadiya

11. Tobacco is a
A. self-pollinated crop
B. cross-pollinated crop
C. often cross pollinated crop
D. allogamous crop
Dr. Indrajay R. Delvadiya

12. SMALL/ CURVED SCISSOR
USE : For cutting the small florets in cereals and small flowers in the crops like Lucerne, Guar etc.
Dr. Indrajay R. Delvadiya

13. Lucerne is a
A. self-pollinated crop
B. cross-pollinated crop
C. often cross pollinated crop
D. allogamous crop
Dr. Indrajay R. Delvadiya

14. LONG STRAIGHT SCISSOR
USE : It is used for clipping, cutting the vegetative parts and large size floral parts in
cereals like Wheat, Sorghum, Bajra, and Tobacco.
Dr. Indrajay R. Delvadiya

15. Sorghum is a
A. self-pollinated crop
B. cross-pollinated crop
C. often cross pollinated crop
D. allogamous crop
Dr. Indrajay R. Delvadiya

16. SHARP POINTER
• USE : It is used for incising the floral parts and for
removing the anthers from the crops like bajra.
Dr. Indrajay R. Delvadiya

17. Dr. Indrajay R. Delvadiya

18. AUTOMIZER
• USE : It is used for spraying the gametocides during emasculation of flowers e.g.
57% ethyl alcohol can be filled in it for killing the pollens of Lucerne
Dr. Indrajay R. Delvadiya

19. Paper Bags
Dr. Indrajay R. Delvadiya

20. BUTTER PAPER BAG
USE : It is used for protecting the individual flowers or small twigs during selfing or crossing
e.g. Mungbean, Tur, Cotton, Okra, Brinjal And Earheads of Sorghum, Bajra, Wheat and Maize.
Being white and semi-transparent it is more suitable than paper bags even anthesis or
blooming can be seen from outside.
Dr. Indrajay R. Delvadiya

21. MUSLIN CLOTH BAG (LARGE SIZE)
•
• USE : To cover the whole plant while selfing or crossing in the
crops like Chillies, Brinjal etc. In large sized plants like Tur it
can be used for protecting individual branch also.
•
Dr. Indrajay R. Delvadiya

22. Dr. Indrajay R. Delvadiya

23. BROWN PAPER BAG
USE : For selfing Bajra, Wheat, Sorghum, Castor etc
Dr. Indrajay R. Delvadiya

24. KITE PAPER BAG(WHITE/RED)
USE : It is used for protecting small size flowers of Pulses, Oilseeds and Other food crops
during selfing and crossing
Dr. Indrajay R. Delvadiya

25. • Which type of bag is most commonly used for
bagging
a. Paper bag
b. Butter paper bag
c. Cloth bag
d. Plastic bag
Dr. Indrajay R. Delvadiya

26. LUGGAGE LABELS (WHITE OR YELLOW)
USE : It is used for tagging the large sized plants like Tur or Castor while rouging or during
selection.
Dr. Indrajay R. Delvadiya

27. SMALL WHITE TAG
USE : It is used for identifying the flower or a small twig during crossing programme. The
detailed information about crossing is written on it with pencil and then it is inserted on
pedicel or peduncle e.g. Cotton, Bajra, Wheat, Sorghum, Sesamum etc.
Dr. Indrajay R. Delvadiya

28. ALUMINIUM LABEL WITH WIRE
• USE : It is used for tagging the flowers in fruit crops
or tree species after crossing. It is also used for
identification of selected trees
Dr. Indrajay R. Delvadiya

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30. Dr. Indrajay R. Delvadiya

31. HAIR BRUSH
USE : It is used for transferring the pollen grains in crops like Castor, Sorghum etc.
ADVANTAGE : Without injuring to stigmas or pollens, pollination is accomplished very
smoothly.
Dr. Indrajay R. Delvadiya

32. U-PINS(U- CLIPS)
USE : It is used for fixing the bags on earheads or flowers to keep the
bag in proper position.
Dr. Indrajay R. Delvadiya

33. WIRE RING AND SMOOTH THREAD
• USE : It is used for selfing in crops like Okra, Sesamum etc.
Thread is used for tying the bud, while ring is inserted in
axis of flowers to identify it. Compared to bags, this method
is more convenient and cheaper
Dr. Indrajay R. Delvadiya

34. YELLOW SAMPLE BAG
USE : For storing the crossed seeds in small quantity
Dr. Indrajay R. Delvadiya

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