What are three possible methods that a eukaryote might ultilize to allow a single DNA sequence to code for at least a portion of more than one protein? Solution There are more than one way that a specific segment of a DNA, called genes, can encode multiple protein types 1. Alternative splicing is a process where exons or portions of exons or noncoding regions within a pre-mRNA transcript are differentially joined or skipped, resulting in multiple protein isoforms being encoded by a single gene. 2. The secend way to of this is use of alternative initiation or termination codons which allows two proteins to be generated where one is equivalent to a fragment of the other. 3. the third way of this is Overlapping genes which occur in the relatively simple situation in which one gene is part of the other. The first half (or second half) of a gene is used independently to specify a protein that represents the first (or second) half of the protein specified by the full gene..