Explain microscope in simplest terms Definition and significance of contrast, absorption, refraction, and scattering to light microscopy I know what contrast is, but what is the significance of absorption, refraction, and scattering of the light to a light microscope? When do all of these come in to play Solution Microscope- Microscope is optical instrument, in which light makes image clear to see microorganism, plant and animal cell etc. Significance of absorbance, scattering and reflection in microscope- In light microscopy abortion play the role to see the object in term to see the colour when visible light (VIBGYOR) put on to object the all waves are absorbed but a particular wave length is reflect by this wave we can see the colour of the object. For example the leaf of the plant look green because all wave are absorbed but green wave length is reflect. In scattering of light microscope the light is scattered by rough or rigid area of the object, from this we obtain size and shape of the object. When put the visible light on object it absorbed, scattered and reflect by ht object at a time by absorbance and reflection we see the colour and by scattering see the shape and size of the object.