Describe how to make 10ml of a 1:25 dilution from a 1 times 10^12 cells/ml stock. What is the
resulting cell concentration?
Solution
= 1/25
Step 1
Take one part stock and add 4 parts water to get 1/5 dilution i.e. 1x 10 12 cells/ml/ 5
= 0.2 1x 10 12 cells/ml
Step 2
Now take one part stock of this cell suspension and add 4 parts water to get 1/5 dilution leading
to 1/25 dilution.
= 0.2 x 10 12 cells/ml/ 5
0.04 xx 10 12 cells/ml the total volume will be 10 ml
b) = 4 x 10 10 cells/ml this is the final cell concentration..
Describe how to make 10ml of a 125 dilution from a 1 times 10^12 cel.pdf
1. Describe how to make 10ml of a 1:25 dilution from a 1 times 10^12 cells/ml stock. What is the
resulting cell concentration?
Solution
= 1/25
Step 1
Take one part stock and add 4 parts water to get 1/5 dilution i.e. 1x 10 12 cells/ml/ 5
= 0.2 1x 10 12 cells/ml
Step 2
Now take one part stock of this cell suspension and add 4 parts water to get 1/5 dilution leading
to 1/25 dilution.
= 0.2 x 10 12 cells/ml/ 5
0.04 xx 10 12 cells/ml the total volume will be 10 ml
b) = 4 x 10 10 cells/ml this is the final cell concentration.
