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Laboratory equipment
Prepared
by Zubair
Khan
Lecturer
MLT
What Is a
Laboratory?
• A laboratory is a room or a place equipped
for the performance of tests,
experimentation, investigative processes .
Medical
laboratory
• A medical laboratory or clinical
laboratory is a laboratory where tests
are done on clinical specimens in order
to get information about the health of a
patient as pertaining to the diagnosis,
treatment, and prevention of disease.
Lab
equipment
Lab. equipment refers to the various tools
used by scientists working in a
laboratory.
Microsco
pyWhat is a microscope?
• Is an optical instrument consisting of a
combination of lenses which magnifies the
image of the object seen through it.
• It is used for the morphological study of a very
small organisms which are not visible by naked
eye.
Micro= small scope=to view
Types of
microscopes
• Simple
• Compoun
d
• Electron
Incubat
or
• Is a device used to grow and maintain
microbiological cultures.
• The incubator maintains optimal temperature,
humidity and other conditions such as the
carbon dioxide (CO2) and oxygen content of
the atmosphere inside.
Incubator
Autocla
ve• An autoclave is a pressure chamber used
to sterilize equipment and supplies by
subjecting them to high pressure saturated
steam at 121 °C for around 15–20 minutes
depending on the size of the load and the
contents.
• Used to sterilize culture media, discard, and
other equipments.
Autoclav
e
Ove
n
• device used in sterilization.
• oven uses dry heat to sterilize.
• It used to sterilize items that might be
damaged by moist heat (e.g., glasswares,
powders, oils).
Laboratory
refrigerator
Is used for a wide variety of purposes
such as:
• maintenance and storage of stock culture
between subculturing periods.
• storage of sterile media to prevent dehydration.
• also used as repository for thermolable solutions,
antibiotics and serums.
Lab. refrigerator
Centrifu
ge
• is an apparatus that rotates at high speed
and separates substances of different
densities.
Balanc
e• used to measure an object’s massto averyhigh
degree of precision.
hot plate / stir
plate
• used to heat and stir
substances.
Magnetic stirring bars
Water
bath
• is a device that maintains water at a
constant temperature.
• It is used in the microbiological laboratory
for incubations.
Biological Safety
Cabinets
• is an enclosed, ventilated laboratory
workspace for safely working with materials
contaminated
with pathogens.
Bunsen
burneris a common piece of laboratory equipment
that produces a single open gas flame, which
is used for heating and sterilization.
Inoculating loops and
needles• Inoculating loops are used to transfer
microorganisms to
growth media or for staining slides.
• The wire forms a small loop with a diameter of about 5
mm.
• The loop of wire at the tip may be
made of platinum or nichrome.
• Needles are straight wires (no loop) used to
pick up bacteria from closely packed colonies
or to inoculate in a very defined area.
• needles commonly used to inoculate semi-
soft media.
Anaerobic
jar
• is an instrument used in the production of
an anaerobic environment.
• This method of anaerobiosisis used to
culture bacteria which die or fail to grow in
presence of oxygen.
Glasswa
reGlass slide and cover slip
• Glass slide:
used to place specimens on to observe under the
microscope.
• Cover slip:
used to cover specimens on a microscope slide.
slide and
coverslip
Glasswa
rePetri dishes
• often used to make agar plates for microbiology studies.
• The dish is partially filled with warm liquid containing agar and a
mixture of specific ingredients that may include:
• nutrients
• blood
• salts
• Carbohydrates
• dyes
• indicators
• amino acids
or
• antibiotics
Glasswa
re
• Pipet and Graduated
Cylinders
• Glass or plastic
• Used to measure liquid volume.
• Graduated in ml.
Graduated Cylinders Pipet
Glasswa
re
Beaker
• Glass or plastic
• Used to stir, heat(if glass) and measure liquid
volume in ml.
Glasswa
re
Flask
• Glassware used to heat and store
substances.
Glasswa
re
Funnel
aids in pouring liquids into small openings
without spilling them.
Glasswa
re• Test tube
used to mix, heat or store
substances.
• Test tube rack
used to hold test tubes.
wash
Bottles
• used to rinse various pieces of
laboratory glassware.
Filter
paper
• special paper used to separate solids
from liquids.
En
d

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Lab Equipment Essentials

  • 2. What Is a Laboratory? • A laboratory is a room or a place equipped for the performance of tests, experimentation, investigative processes .
  • 3. Medical laboratory • A medical laboratory or clinical laboratory is a laboratory where tests are done on clinical specimens in order to get information about the health of a patient as pertaining to the diagnosis, treatment, and prevention of disease.
  • 4. Lab equipment Lab. equipment refers to the various tools used by scientists working in a laboratory.
  • 5. Microsco pyWhat is a microscope? • Is an optical instrument consisting of a combination of lenses which magnifies the image of the object seen through it. • It is used for the morphological study of a very small organisms which are not visible by naked eye. Micro= small scope=to view
  • 6. Types of microscopes • Simple • Compoun d • Electron
  • 7. Incubat or • Is a device used to grow and maintain microbiological cultures. • The incubator maintains optimal temperature, humidity and other conditions such as the carbon dioxide (CO2) and oxygen content of the atmosphere inside.
  • 9. Autocla ve• An autoclave is a pressure chamber used to sterilize equipment and supplies by subjecting them to high pressure saturated steam at 121 °C for around 15–20 minutes depending on the size of the load and the contents. • Used to sterilize culture media, discard, and other equipments.
  • 11. Ove n • device used in sterilization. • oven uses dry heat to sterilize. • It used to sterilize items that might be damaged by moist heat (e.g., glasswares, powders, oils).
  • 12.
  • 13. Laboratory refrigerator Is used for a wide variety of purposes such as: • maintenance and storage of stock culture between subculturing periods. • storage of sterile media to prevent dehydration. • also used as repository for thermolable solutions, antibiotics and serums.
  • 15. Centrifu ge • is an apparatus that rotates at high speed and separates substances of different densities.
  • 16.
  • 17. Balanc e• used to measure an object’s massto averyhigh degree of precision.
  • 18. hot plate / stir plate • used to heat and stir substances. Magnetic stirring bars
  • 19. Water bath • is a device that maintains water at a constant temperature. • It is used in the microbiological laboratory for incubations.
  • 20. Biological Safety Cabinets • is an enclosed, ventilated laboratory workspace for safely working with materials contaminated with pathogens.
  • 21.
  • 22. Bunsen burneris a common piece of laboratory equipment that produces a single open gas flame, which is used for heating and sterilization.
  • 23. Inoculating loops and needles• Inoculating loops are used to transfer microorganisms to growth media or for staining slides. • The wire forms a small loop with a diameter of about 5 mm. • The loop of wire at the tip may be made of platinum or nichrome.
  • 24. • Needles are straight wires (no loop) used to pick up bacteria from closely packed colonies or to inoculate in a very defined area. • needles commonly used to inoculate semi- soft media.
  • 25.
  • 26. Anaerobic jar • is an instrument used in the production of an anaerobic environment. • This method of anaerobiosisis used to culture bacteria which die or fail to grow in presence of oxygen.
  • 27.
  • 28. Glasswa reGlass slide and cover slip • Glass slide: used to place specimens on to observe under the microscope. • Cover slip: used to cover specimens on a microscope slide.
  • 30. Glasswa rePetri dishes • often used to make agar plates for microbiology studies. • The dish is partially filled with warm liquid containing agar and a mixture of specific ingredients that may include: • nutrients • blood • salts • Carbohydrates • dyes • indicators • amino acids or • antibiotics
  • 31. Glasswa re • Pipet and Graduated Cylinders • Glass or plastic • Used to measure liquid volume. • Graduated in ml.
  • 33. Glasswa re Beaker • Glass or plastic • Used to stir, heat(if glass) and measure liquid volume in ml.
  • 34. Glasswa re Flask • Glassware used to heat and store substances.
  • 35. Glasswa re Funnel aids in pouring liquids into small openings without spilling them.
  • 36. Glasswa re• Test tube used to mix, heat or store substances. • Test tube rack used to hold test tubes.
  • 37. wash Bottles • used to rinse various pieces of laboratory glassware.
  • 38. Filter paper • special paper used to separate solids from liquids.
  • 39. En d