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Laboratory equipment
Prepared by
Samira fattah
Assis. Lec.
College of health sciences-HMU
Lecture 2
What Is a Laboratory?
• A laboratory is a room or a place equipped for
the performance of tests, experimentation,
investigative processes .
Medical laboratory
• A medical laboratory or clinical laboratory is
a laboratory where tests are done on clinical
specimens in order to get information about
the health of a patient as pertaining to the
diagnosis, treatment, and prevention of
disease.
Lab equipment
Lab. equipment refers to the various tools used
by scientists working in a laboratory.
Microscopy
What is a microscope?
• Is an optical instrument consisting of a combination
of lenses which magnifies the image of the object
seen through it.
• It is used for the morphological study of a very small
organisms which are not visible by naked eye.
Micro= small scope=to view
Types of microscopes
• Simple
• Compound
• Electron
Incubator
• Is a device used to grow and maintain microbiological
cultures.
• The incubator maintains optimal temperature,
humidity and other conditions such as the carbon
dioxide (CO2) and oxygen content of the atmosphere
inside.
Incubator
Autoclave
• An autoclave is a pressure chamber used
to sterilize equipment and supplies by subjecting
them to high pressure saturated steam at 121 °C for
around 15–20 minutes depending on the size of the
load and the contents.
• Used to sterilize culture media, discard, and other
equipments.
Autoclave
Oven
• device used in sterilization.
• oven uses dry heat to sterilize.
• It used to sterilize items that might be damaged by
moist heat (e.g., glasswares, powders, oils).
Laboratory refrigerator
Is used for a wide variety of purposes such as:
• maintenance and storage of stock culture between
subculturing periods.
• storage of sterile media to prevent dehydration.
• also used as repository for thermolable solutions, antibiotics
and serums.
Lab. refrigerator
Centrifuge
• is an apparatus that rotates at high speed
and separates substances of different densities.
Balance
• used to measure an object’s mass to a very high
degree of precision.
hot plate / stir plate
• used to heat and stir substances.
Magnetic stirring bars
Water bath
• is a device that maintains water at a constant
temperature.
• It is used in the microbiological laboratory for
incubations.
Biological Safety Cabinets
• is an enclosed, ventilated laboratory workspace for
safely working with materials contaminated
with pathogens.
Bunsen burner
is a common piece of laboratory equipment that
produces a single open gas flame, which is used for
heating and sterilization.
Inoculating loops and needles
• Inoculating loops are used to transfer microorganisms to
growth media or for staining slides.
• The wire forms a small loop with a diameter of about 5 mm.
• The loop of wire at the tip may be made
of platinum or nichrome.
• Needles are straight wires (no loop) used to pick up
bacteria from closely packed colonies or to inoculate
in a very defined area.
• needles commonly used to inoculate semi-soft
media.
Anaerobic jar
• is an instrument used in the production of an
anaerobic environment.
• This method of anaerobiosis is used to culture
bacteria which die or fail to grow in presence
of oxygen.
Glassware
Glass slide and cover slip
• Glass slide:
used to place specimens on to observe under the microscope.
• Cover slip:
used to cover specimens on a microscope slide.
slide and coverslip
Glassware
Petri dishes
• often used to make agar plates for microbiology studies.
• The dish is partially filled with warm liquid containing agar and a mixture of
specific ingredients that may include:
• nutrients
• blood
• salts
• Carbohydrates
• dyes
• indicators
• amino acids
or
• antibiotics
Glassware
• Pipet and Graduated Cylinders
• Glass or plastic
• Used to measure liquid volume.
• Graduated in ml.
Graduated Cylinders Pipet
Glassware
Beaker
• Glass or plastic
• Used to stir, heat(if glass) and measure liquid volume
in ml.
Glassware
Flask
• Glassware used to heat and store substances.
Glassware
Funnel
aids in pouring liquids into small openings without
spilling them.
Glassware
• Test tube
used to mix, heat or store substances.
• Test tube rack
used to hold test tubes.
wash Bottles
• used to rinse various pieces of laboratory
glassware.
Filter paper
• special paper used to separate solids from
liquids.
End

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labequipments-151023192518-lva1-app6891.pdf

  • 1. b Laboratory equipment Prepared by Samira fattah Assis. Lec. College of health sciences-HMU Lecture 2
  • 2. What Is a Laboratory? • A laboratory is a room or a place equipped for the performance of tests, experimentation, investigative processes .
  • 3. Medical laboratory • A medical laboratory or clinical laboratory is a laboratory where tests are done on clinical specimens in order to get information about the health of a patient as pertaining to the diagnosis, treatment, and prevention of disease.
  • 4. Lab equipment Lab. equipment refers to the various tools used by scientists working in a laboratory.
  • 5. Microscopy What is a microscope? • Is an optical instrument consisting of a combination of lenses which magnifies the image of the object seen through it. • It is used for the morphological study of a very small organisms which are not visible by naked eye. Micro= small scope=to view
  • 6. Types of microscopes • Simple • Compound • Electron
  • 7. Incubator • Is a device used to grow and maintain microbiological cultures. • The incubator maintains optimal temperature, humidity and other conditions such as the carbon dioxide (CO2) and oxygen content of the atmosphere inside.
  • 9. Autoclave • An autoclave is a pressure chamber used to sterilize equipment and supplies by subjecting them to high pressure saturated steam at 121 °C for around 15–20 minutes depending on the size of the load and the contents. • Used to sterilize culture media, discard, and other equipments.
  • 11. Oven • device used in sterilization. • oven uses dry heat to sterilize. • It used to sterilize items that might be damaged by moist heat (e.g., glasswares, powders, oils).
  • 12.
  • 13. Laboratory refrigerator Is used for a wide variety of purposes such as: • maintenance and storage of stock culture between subculturing periods. • storage of sterile media to prevent dehydration. • also used as repository for thermolable solutions, antibiotics and serums.
  • 15. Centrifuge • is an apparatus that rotates at high speed and separates substances of different densities.
  • 16.
  • 17. Balance • used to measure an object’s mass to a very high degree of precision.
  • 18. hot plate / stir plate • used to heat and stir substances. Magnetic stirring bars
  • 19. Water bath • is a device that maintains water at a constant temperature. • It is used in the microbiological laboratory for incubations.
  • 20. Biological Safety Cabinets • is an enclosed, ventilated laboratory workspace for safely working with materials contaminated with pathogens.
  • 21.
  • 22. Bunsen burner is a common piece of laboratory equipment that produces a single open gas flame, which is used for heating and sterilization.
  • 23. Inoculating loops and needles • Inoculating loops are used to transfer microorganisms to growth media or for staining slides. • The wire forms a small loop with a diameter of about 5 mm. • The loop of wire at the tip may be made of platinum or nichrome.
  • 24. • Needles are straight wires (no loop) used to pick up bacteria from closely packed colonies or to inoculate in a very defined area. • needles commonly used to inoculate semi-soft media.
  • 25.
  • 26. Anaerobic jar • is an instrument used in the production of an anaerobic environment. • This method of anaerobiosis is used to culture bacteria which die or fail to grow in presence of oxygen.
  • 27.
  • 28. Glassware Glass slide and cover slip • Glass slide: used to place specimens on to observe under the microscope. • Cover slip: used to cover specimens on a microscope slide.
  • 30. Glassware Petri dishes • often used to make agar plates for microbiology studies. • The dish is partially filled with warm liquid containing agar and a mixture of specific ingredients that may include: • nutrients • blood • salts • Carbohydrates • dyes • indicators • amino acids or • antibiotics
  • 31. Glassware • Pipet and Graduated Cylinders • Glass or plastic • Used to measure liquid volume. • Graduated in ml.
  • 33. Glassware Beaker • Glass or plastic • Used to stir, heat(if glass) and measure liquid volume in ml.
  • 34. Glassware Flask • Glassware used to heat and store substances.
  • 35. Glassware Funnel aids in pouring liquids into small openings without spilling them.
  • 36. Glassware • Test tube used to mix, heat or store substances. • Test tube rack used to hold test tubes.
  • 37. wash Bottles • used to rinse various pieces of laboratory glassware.
  • 38. Filter paper • special paper used to separate solids from liquids.
  • 39. End