Cleaning of laboratory new glassware is not as simple as washing the dishes. Here is how to wash your lab glassware so that you will not waste your chemical solution or laboratory experiment as well as time consumed during the experiment.

1. CLEANING OF LABORATORY NEW
GLASSWARE
By: Rahul Gautam
M.Sc. Biochemistry
Certified MLT & Phlebotomy Trainer

2. OBJECTIVES
• PRINCIPLE
• REQUIREMENTS
• PROCEDURE

3. CLEANING OF LABORATORY NEW GLASSWARE
Principle : Lab glasswareis usuallymanufactured
fromborosilicateglass (hard glass).
It isresist tothe actionofchemicalswiththe exceptionof
hydrofluoric acidand phosphoric acid.
It can withstandmechanicalbreakageand sudden
change oftemperature.
Soda limeglassware(soft glass)is not as goodas
borosilicateglassware.

4. REQUIREMENTS
• Soda lime glassware ( soft glassware)
• Borosilicate glassware or hard glassware.
• 5% HCL
• Tap water
• Distilled water
• 2% detergent solution

5. PROCEDURE
FOR SODA LIME OR SOFT GLASSWARE
1. The free alkali present on soda lime glassware is neutralized
by treating overnight with 5% HCL

6. 2. Rinse the glassware several times in tap water.

7. 3.After rinsing with tap water rinse the glassware several
times in distilled water .

8. 4. Dry the glassware in hot air oven

9. • Note : To test that the free alkali has been neutralized ,
Autoclave the glassware in neutral distilled water and when
cool check the pH of
water.

10. THANK YOU