A Master Level Thesis about Extraction of Eucalyptus camaldulensis using GC-MS Analysis.

1. IDENTIFYING THE
CHEMICAL COMPOSITION
OF ESSENTIAL OIL OF
EUCALYPTUS
CAMALDULENSIS USING GC-
MS ANALYSIS
Muhammad Usama Maoud
University of Education, Lahore, Campus D. G. Khan
BS-Chemistry (2017-21)
Supervisor: Dr. Riaz Hussain

2. Objectives
• Identify Novel Natural Compounds in Eucalyptus
camaldulensis
• Analyzing The GC-MS Graphs of the compounds of
Eucalyptus camaldulensis
• What are the medicinal uses of the compounds
Eucalyptus camaldulensis

3. Introduction
 Scientific Name: Eucalyptus camaldulensis
 Common Name: River Redgum
 The precise nickname, camaldulensis, evolves through a cultured
tree in Camalduli, in Tuscany, Italy.
 It’s Essential oil is widely used as an anti-bacterial medicine.
 It’s Compounds also served as Anti-Fungal medicine.
 It’s Fumigant effects make it a commercial Plant.
 It’s essential oil found in the leaves is a powerful antiseptic and is
used for relieving coughs and colds, sore throats.
 It’s essential oil is a common ingredient in many over the counter
cold remedies.
 It only grow only near wetlands and permits other plant’s growth.
 Only Four compounds obtained in Hexane Extract.

4. •Domain: Eukaryota
•Kingdom: Plantae
•Phylum: Spermatophyta
•Subphylum: Angiospermae
•Class: Dicotyledonae
•Order: Myrtales
•Family: Myrtaceae
•Genus: Eucalyptus
•Species: Eucalyptus camaldulensis
Introduction continued
Taxonomic Tree

5. Introduction continued
Scientific Name & Common Name
• Scientific Name
• Eucalyptus camaldulensis.
• Common Name
• Red gum

6. Methodology
Collection Pure
fresh Leaves collected,
dried and grinded to a
Fine Powder.
Apparatus
Funnel,
Beaker,
Soxhlet
Extractor,
Round
Bottom
Flask,
Condenser
and Heating
Mentle.
Procedure: I made a thimble of filter paper
and put the 100 grams of powder of leaves
in it, then I set a condenser and filled it with
cool water and Set apparatus and took
600ml Hexane in Round Bottom Flask and
heated by Heating Mentle till boiling of
hexane started. I continued it till the whole
vaporized hexane was filled in extractor and
recovered through siphon tube in the round
bottom flask. I separated out flask and set it
dry till whole hexane vaporized and solid
sample left behind.

7. Results & Discussion
Peak # R.T Compound Name M.F Structure M.W
g/mol
1 21.844 60479059,12-Octadecadienoicacid,methylester,(E,E) C19H37O2
297.5
2 22.387 2396113Docosanoicacid,methylester(CAS) C23H46O2
354.6
3 27.795 3968083,3-Dibutoxy-1,1,1,5,5,5-hexamethyltrisiloxane C14H36Si3O2 320.69
4 29.045 1276622Thiocolchicine
C22H25NO5S
415.5

8. Results & Discussion Continued
GAS CHROMATOGRAPHY
Basic Principle of gas chromatography
• The sample solution injected into the
instrument enters a gas stream which
transports the sample into a separation
tube known as the "column."

9. GC Analysis Chromatogram Shows a two-dimensional plot with the
ordinate axis giving concentration in terms of the detector response, and
the abscissa represents the time. The detector gives a response as a peak
whose height should be ideally dependent on the concentration of the
particular component.
Results & Discussion Continued
GC Analysis Chromatogram

10. Results & Discussion Continued
MASS SPECTROMETRY
Basic Principle
• The basic principle of mass spectrometry
(MS) is to generate ions from either
inorganic or organic compounds by any
suitable method, to separate these ions by
their mass-to-charge ratio (m/z) and to
detect them qualitatively and quantitatively
by their respective m/z and abundance.

11. Results & Discussion Continued
Mass Spectrum
A mass spectrum is a plot of the ion signal as a function of the
mass-to-charge ratio. These spectra are used to determine the
elemental or isotopic signature of a sample, the masses of
particles and of molecules, and to elucidate the chemical identity
or structure of molecules and other chemical compounds.

12. Conclusion
The GC-MS analysis of n-hexane fraction of the leaf of E.camaldulensis afforded four well-
known compounds including 60479059,1,2 Octadecadienoic acid , methylester,(E,E),
2396113Docosanoicacid,methylester(CAS), 3968083,3-Dibutoxy-1,1,1,5,5,5-
hexamethyltrisiloxane, 1276622Thiocolchicine having Retention Time 21.844, 22.387, 27.795,
29.045 respectively.
These compounds had been reported to exhibit various biological activities; hence hexane
fraction of the leaf of E.camaldulensis could serve as a source for these bioactive principles
which might be employed as ingredients for formulation of novel drug.

13. Recommendations
Extraction Should also be done in Organic Solvents Other
than the Hexane to identify more compounds.
In near future the Eucalyptus camaldulensis will be
medicinal plant and by further technique like hydro distillation
one can obtain further useful compounds like Eucalyptol.

14. Summary
Objectives
Introduction
Methodology
Results & Discussion
Conclusion
Recommendations

15. Acknowledgement
On completion of my thesis practical and
documentation work,
I am pretty Thankful to my Supervisor Dr. Riaz Hussain
AP/UE/DGK whose office door always opened for me.
I pay thanks to Dr. Arfan Yawar Principal UE D.G.Khan
who encouraged & supported me .
I am grateful to the University of Education, Lahore,
campus D. G. Khan Faculty of Chemistry Department
who gave me a chance to complete thesis.