Biofilm formation

1. BIOFILMFORMATION
Presented by:
Md Al amin Bhuiyan Shuvo (14103067)
PJ Eric (14103070)
Pritom Roy (14103075)
Md.Humayon Kabir (14103079)
Sajuti Akter Pakhe (14103095)

2. BIOFILMS
Biofilm is an assemblage of microbial cells that is
irreversibly associated with a surface and enclosed in
a matrix of primarily polysaccharide material.
structure EPS surface
A structured
community of
bacterial cell
Enclosed in a self produced
extracellular polymeric matrix
Adherent to an inert or living
surface

3. WHEN DO MICROBES DECIDE TO FORM BIOFILMS
 Mainly when they are capable of recognition
of specific or non-specific attachment sites
on a surface
 Nutritional cues
 Exposure of planktonic cells to sub-
inhibitory concentrations of antibiotics

4. INGREDIENTS TO PREPARE A BIOFILM
Celular materials:
 bacteria
 parasites
 fungus
 virus
Non cellular materials:
( extra cellular polymer)
 mineral crystals
 corrosion particles
 clay or slit particles
 blood components etc
 polysachharides
 proteins

5. CHARACTERISTICS OF A SURFACE
Liquid surface Solid surface
Living surface:
Leaves, wounds,
Post surgical sites,
Marine invertebrates, etc
Non living surface:
Industrial setting,
Hospital setting,
Indwelling medical devices,
Rocks/boats in sea,
Toothbrush etc

6. STEPS IN THE FORMATION OF BIOFILM
• STEP 1: Initial / reversible attachment -
binding of 1st colonists
• STEP 2: Irreversible attachment –
they anchor themselves permanently using pili
• STEP 3: Maturation 1 –
inter-communication through quorum sensing
• STEP 4: Maturation 2 / Development –
final stage of modification
• STEP 5: Dispersion –
essential stage for biofilm dispersion and life-cycle
• Role of enzymes dispersin B and deoxyribonucleases in step 5 and medical application of
cis-2-decenoic acid

7. BIOFILM DEVELOPMENT
Phases of Biofilm growth
 Attachment : Adhesion
 Growth : Proliferation and Differentiation
 Detachment

8. ATTACHMENT AND GROWTH

9. DETACHMENT
Abrasion
 Direct physical contact with biofilm structure
Erosion
 Shear forces exerted on the biofilm
Sloughing
 Result of chemical changes
 Regulated by the biofilm community
Enzymatic EPS degradation

10. FACTORS AFFECTING THE SHAPE OF BIOFILM

11. BIOFILM PROBLEMS IN PHARMACEUTICAL MANUFACTURING
•Product quality
•Process problems
•Sampling problems
•Lab analysis problems
•Control problems

12. HURDLE TECHNOLOGY FOR BIOFILM PREVENTION
•Process maintenance
•Sanitary equipment, sanitary layout
•Process water flush parameters and fluid dynamics
•Process surface cleaning
•Process sanitization
•Raw material cleanliness
•UV treatment, filtration
•Antimicrobials, preservatives
Hurdle technology is the application of a series of “barriers”or “hurdles”in
the manufacturing process that reduce the level of organisms at each
barrier,