![TEMPLATE DESIGN © 2008
www.PosterPresentations.com
Morven Gannon - C12760661
DT710 - BSc in Medical Device Innovation
Department of Manufacturing Engineering
Dublin Institute of Technology
Introduction
References
Application 1
[1] Wachsmann-Hogiu et al. Current Opinion in Biotechnology (2009) 20: 63-73
[2] Ghita et al. EPJ Techniques and Instrumentation (2015) 2:6
[3] Hauser et al. Advanced Drug Delivery Reviews 89 (2015) 27:15
[4] Pascut et al. Biochimica et Biophysica Acta 1830 (BBA) (2013) 3517 – 3524
[5] http://www.eurostemcell.org/factsheet/mesenchymal-stem-cells-other-bone-
marrow-stem-cells 3/12/15
RAMEN SPECTROSCOPY APPLICATION IN STEM-CELL ENGINEERING
• Assessing differentiation in neural progenitor stem
cells that can generate neurons and glial cells:
• This research is dedicated to treatment of
Parkinson’s, Alzheimer’s, Strokes and the chronic
inflammatory and trauma they cause in the CNS
and PNS with the replacement of in-vivo neural
and glial cells [2]
Stem cell applications have enormous potential in
regenerative therapy and already provide
engineered cell tissue to repair diseased cells,
replace dead ones and build scaffold structure grafts
encouraged to build new tissue. A great deal of
further research is required to make stem cell
applications affordable and practical enough to
encourage everyday usage in the medical sector
[1]. It should be:
• Non-destructive (without the toxins of fluorescent
labelling)
• Repeatable (time relative)
Raman Microscopic Spectroscopy (RMS) provides a
chemical fingerprint of a molecular particle by
reading the scattered in-elastic photons from a
monochromatic laser light source.
• This is done in stem cell research with a Raman
spectrometer coupled with an inverted microscope
and is more relevant recently due to technological
improvements.
Figure 2: RMS results for Neural Progenitor Cells differentiation (A)
displaying the RNA content difference in the cytoplasm (B) [2]
Figure 1: Typical RMS configuration to monitor spatial changes over
time in live cultured stem cells [2]
Application 2
• Recording and interpreting over a timespan the
mineralisation of bone nodules in-vitro using
cultured mesenchymal stem cells (MSC)
• RMS can observe the proliferation and
differentiation over time to assess the growth of the
nodule forming osteoblasts [4]
Figure 3: Time course RMS of mesenchymal stem cells differentiation
into osteoblasts. The spike forming at 1000 cm-1 indicating the
growing levels of hydroxyapatite, the scaffold for osteocytes [4]
Application 3
• Refining the process of in-vitro differentiation of
cardiac heart tissue (cardiomyocytes – CM) grown
from human embryonic stem cells (EBS), and
potentially dictate the differentiation:
Figure 4: Time course embryonic bodies differentiating into cardiac muscle [2]
Brightfield
Imaging
Figure 3: Differentiation path from MSC to connective tissue[5]](https://image.slidesharecdn.com/ramenspectroscopyposter-160707084928/85/Ramen-spectroscopy-poster-1-320.jpg)
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- 1. TEMPLATE DESIGN © 2008 www.PosterPresentations.com Morven Gannon - C12760661 DT710 - BSc in Medical Device Innovation Department of Manufacturing Engineering Dublin Institute of Technology Introduction References Application 1 [1] Wachsmann-Hogiu et al. Current Opinion in Biotechnology (2009) 20: 63-73 [2] Ghita et al. EPJ Techniques and Instrumentation (2015) 2:6 [3] Hauser et al. Advanced Drug Delivery Reviews 89 (2015) 27:15 [4] Pascut et al. Biochimica et Biophysica Acta 1830 (BBA) (2013) 3517 – 3524 [5] http://www.eurostemcell.org/factsheet/mesenchymal-stem-cells-other-bone- marrow-stem-cells 3/12/15 RAMEN SPECTROSCOPY APPLICATION IN STEM-CELL ENGINEERING • Assessing differentiation in neural progenitor stem cells that can generate neurons and glial cells: • This research is dedicated to treatment of Parkinson’s, Alzheimer’s, Strokes and the chronic inflammatory and trauma they cause in the CNS and PNS with the replacement of in-vivo neural and glial cells [2] Stem cell applications have enormous potential in regenerative therapy and already provide engineered cell tissue to repair diseased cells, replace dead ones and build scaffold structure grafts encouraged to build new tissue. A great deal of further research is required to make stem cell applications affordable and practical enough to encourage everyday usage in the medical sector [1]. It should be: • Non-destructive (without the toxins of fluorescent labelling) • Repeatable (time relative) Raman Microscopic Spectroscopy (RMS) provides a chemical fingerprint of a molecular particle by reading the scattered in-elastic photons from a monochromatic laser light source. • This is done in stem cell research with a Raman spectrometer coupled with an inverted microscope and is more relevant recently due to technological improvements. Figure 2: RMS results for Neural Progenitor Cells differentiation (A) displaying the RNA content difference in the cytoplasm (B) [2] Figure 1: Typical RMS configuration to monitor spatial changes over time in live cultured stem cells [2] Application 2 • Recording and interpreting over a timespan the mineralisation of bone nodules in-vitro using cultured mesenchymal stem cells (MSC) • RMS can observe the proliferation and differentiation over time to assess the growth of the nodule forming osteoblasts [4] Figure 3: Time course RMS of mesenchymal stem cells differentiation into osteoblasts. The spike forming at 1000 cm-1 indicating the growing levels of hydroxyapatite, the scaffold for osteocytes [4] Application 3 • Refining the process of in-vitro differentiation of cardiac heart tissue (cardiomyocytes – CM) grown from human embryonic stem cells (EBS), and potentially dictate the differentiation: Figure 4: Time course embryonic bodies differentiating into cardiac muscle [2] Brightfield Imaging Figure 3: Differentiation path from MSC to connective tissue[5]