Coated fiber scaffold for three dimensional cell culture of
neural cells, this is the important experiment for my
Three-dimensional cell and tissue culture system, in this
research, collagen is used for encapsulated for cell
Neural stem cell-collagen-bioreactor system to construct a
functional embryonic brain-like tissue. These neural
progenitor cells in the bioreactor were held arrest collagen
Apart from these neural stem cells and neural cells in three-
dimensional collagen matrix with a lot of encapsulated is
under patent protection.
 LIU, J., Pekny, M., Puschmann, T. B., Zanden, C., (2012), Coated fiber scaffold for three dimensional cell
culture of neural cells, publication number: WO2012158120 A1.
 Naughton, A. B., Naughton, K. G., (1989), Three-dimensional cell and tissue culture system, publication number:
 Ma, W., (2009), Neural stem cell-collagen-bioreactor system to construct a functional embryonic brain-like tissue,
publication number: US7588938 B2
Which is the best way?
O'Connor and colleagues observed entrapment of cortical neurons with collagen matrix
showed that exhibit behavior closer to the truth in their experiment .
 O’Connor, S. M., Andreadis, J.D., Shaffer, K. M., Pancrasio, J.J. and Stenger, D.A., (1999) immobilisation of neural cells in
three-dimensional matrices for biosensor applications. Laboratory for Biosensors and Biomaterials, Center for Bio:Molecular
Science and Engineering, Na6al Research Laboratory, Code6910, 4555O6erlook A6enue SW, Washington, DC20375, USA.
• Entrapment on sugar
•Conformational properties of
the cells can not fully protect
Entrapment on collagen matrix
have large pores
simple gel formation
have high mechanical
injectable forms are unable to
the mechanical and shape
 O’Connor, S. M., Stenger, D. A., Shaffer, K. M., Ma, W.,
(2001), Survival and neurite outgrowth of rat cortical
neurons in three-dimensional agarose and collagen gel
matrices, Neuroscience Letters 304 (2001) 189-193.
Washington, D.C. 20375, USA
 Bellamkonda, R., Ranieri, J. P. Bouche, N. Aebischer, P.
(1995), Hydrogel-based three-dimensional matrix for
neural cells, Division of Surgical Research, Centre
Hospitalier Universitaire Vaudois, Lausanne University
Medical School, Switzerland.
WHY rotating wall
environment. All forces balance each
•Facilitate diffusion of nutrients.
•Matrix synthesis speeds.
•Redifferentiation and stimulates
•Properly mixing reduces the
Mathematical Model defining to Experimental Design
Sugar poly(acrylate) hydrogel
Temperature ( 4 oC is ideal for
I can not use RSM technique for these
Response: Cell activity
•Sugar poly(acrylate) hydrogel
•Temperature ( 4 oC is ideal for
•No control group.
•Results from samples taken and the effect of the parameters is according to the
•Data analysis will be based on microscopic observations and viable counts.
•Independent variables changing causes a negative effect on cell growth, cause in optimal
conditions cells exhibit growth.
 Borkenhagen, M., Clemence, J.-F., Sigrist, H., Aebischer, P., (1998)Three-dimensional extracellular matrix engineering in the nervous system. J.
Biomed. Mater. Res. 40, 392 – 400
Operating Mode: Fed Batch
Done during the assay culture system
measurements, increase in the
number of cells per day are expected
to occur. This is estimated supported
with current research
Samples of the cells to the cell due to-
day there is an increase in the
•Cullen, D. K., Vukasinovic, J., Glezer, A. (2007),“Microfluidic Engineered High Cell Density Three-
Dimensional Neural Cultures,” J. Neural Eng., Vol. 4, No. 2, 2007, pp. 159–172.
In this experiment results due to:
•The increase in cell number and cell growth