Microfluidic cell culture techniques

1. ORGAN ON A CHIP
Presented by
Sandeep Ganesh Grandhi
219603
Department of pharmacology
Shri Vishnu College of Pharmacy (Autonomous)
Affiliated to Andhra Univ., Visakhapatnam; Approved by AICTE and PCI, New Delhi, and recognised by APSCHE
1

2. INTRODUCTION
An organ on a chip is a multi channel 3-d microfluidic cell culture
chip that simulates the activities, mechanics and physiological
response of entire organs and organ systems.
Organ on a chip is a device which can mimic cell responses more
accurately than regular in vitro cell cultures.
Each organ chip, such as the lung, liver, intestine or brain, is about the
size of a AA battery.
The chip’s transparency allows researchers to see the organ’s
functionality, behaviour and response, at cellular and molecular level.

3. The idea is to recreate the
smallest functional unit of
any particular organ in a
micro environment that
closely imitates the human
body.
THE IDEA OF DESIGN
Huh et al., 2013 Nature protocol

4. DESIGN CONCEPTS OF ORGANS ON A CHIP
Generation of fluid shear stress
Generation of dynamic mechanical stress
Generation of concentration gradient

5. PRIMARY FOCUS OF ORGAN ON A CHIP TECHNOLOGY
The multicellular vascular or epithelial interfaces of organs
Barriers in tissues
functional properties of an organ; and the systematic interaction of
multiple organs

6. FUNCTIONAL TISSUES
Huh et al., 2011; Bhatiya et al., 2014

7. FABRICATION METHODS FOR MICROFLUIDIC CHIPS
Huh et al., 2011; Bhatiya et al., 2014

8. DESIGN FOR LUNG ON A CHIP
Huh et al., 2013 Nature protocol

9. THE LUNG ON A CHIP
Huh et al., 2013 Nature protocol

10. HUMAN ON A CHIP

11. HUMAN ON A CHIP

12. APPLICATIONS
Understanding biological mechanisms for novel drug targets
Providing insight into disease processes
Predicting the efficacy and safety of new drug candidates
Screening using much lower drug amounts
Toxicity tests
Personalized health applications

13. DISADVANTAGES
Every property of an organ cannot be measured.
It is sometimes not possible to study complete organ.
Drug action during pregnancy cannot be studied.

14. CONCLUSION
The use of an organ on a chip model in the drug development process
can be beneficial in either the basic research stage or the preclinical
stage.
This could change the drug development process by replacement of
the animal models.

15. Thank you