Non-enzymatic glucose sensors are needed to efficiently monitor diabetes without skin punctures. This study created a flexible, transparent glucose sensor using copper oxide nanowires (CuO NWs). CuO NWs were produced via electroplating and annealing copper, then sonicating and filtering to deposit on polyethylene terephthalate film. Scanning electron microscopy, energy dispersive X-ray spectroscopy and Raman spectroscopy confirmed the presence of CuO NWs averaging 35.5 nm. The sensor detected current increases relative to glucose concentrations from 0-12 mM at various voltages, both before and after bending, demonstrating flexibility. The sensor was selective for glucose even in solutions containing other biological compounds.