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Sample collection for culture test
- 1. SAMPLE COLLECTION FOR CULTURE TEST By Maleeha Fatima
- 2. CULTURE TEST ▪ Medical laboratory test used to identify the presence of an microorganism in patient’s sample ▪ Culture- observable growth ▪ Media- substance required for the growth of culture
- 3. SAMPLE COLLECTION ▪ Main and important step ▪ Collected by well trained expert ▪ If does not collect properly, may leads to false positive result or damage to patient
- 5. VENIPUNCTURE ▪ Collected from vein ▪ For laboratory testing i.e. culture test ▪ Performed by phlebotomist ▪ Easier than arterial sampling ▪ Drawn from the vein of top of the hand or inside of the elbow
- 6. REQUIREMENTS
- 7. METHOD ▪ Medical history of patient ▪ Find vein ▪ Wear disposable gloves ▪ Tie tourniquet on patient’s arm ▪ Wipe the blood collection area with alcohol pad ▪ With the help of syringe, collect venous blood following aseptic precautions ▪ Then inoculate the sample immediately into bottle containing BHI, after disinfect the bottle
- 8. Brain Heart Infusion (BHI) BHI broth is a highly nutritive media for cultivating wide variety of microorganisms.
- 9. ▪ Remove the cap and disinfect the rubber cork with alcohol pad. ▪ Replace the needle with new one and insert the syringe in rubber cork. Don’t press the syringe as it leads to hemolysis. ▪ Mix the blood slowly in BHI broth bottle as not to allow the blood to clot in culture media because bacteria will get trapped in the clot. ▪ Incubate the blood culture bottle at 37°C for about 7 days and observe regularly for turbidity. ▪ If turbidity occurs, then perform sub- culture.
- 10. NOTE ▪ Blood should be collect before antimicrobial treatment. ▪ The ratio of blood-to-nutrient broth must be correct. ▪ Check the sterility of BHI bottle before inoculation of blood. ▪ If the media is turbid, discard the bottle.
- 11. URINE SAMPLE COLLECTION ▪ Label a sterile, screw-top container. ▪ Wash your hands. ▪ Collect “mid-stream” urine in the container. ▪ Screw the lid of the container properly. ▪ Wash your hands thoroughly.
- 12. MID-STREAM URINE ▪ A mid-stream urine sample means you don’t collect the first or last part of urine that comes out. This reduces the risk of the sample being contaminated with bacteria from: your hands, the skin around the urethra. ▪ This is the preferred type of specimen for culture and sensitivity testing because of the reduced incidence of cellular and microbial contamination.
- 13. NOTE ▪ Processed the sample within 2 hours directly after collection. ▪ Refrigerated samples must be processed within 24 hours. ▪ Sample with borate preservative must be processed with in 24 hours. ▪ If the sample will not tested timely, it may leads to false positive results.
- 14. Thank you!