5. Main active constituents
▪ Dried garlic powder 200 mg (Alliin) (precursor to Allicin)
▪ Allicin (sulphur containing compound)
6. Uses of Garlic
▪ to boost the immune system.
▪ lowerTotal and LDL cholesterol.
▪ antioxidants protects against oxidative damage.
▪ prevent many types of cancer
7. Uses of Garlic
▪ Improve Bone Health
▪ reduce fatigue.
▪ reduce blood pressure.
▪ protect against organ damage from heavy metal toxicity.
8. Folk Uses of Garlic
▪ repel mosquitoes .
▪ increase your immunity.
▪ heal cold sores.
9. Folk Uses of Garlic
▪ Garlic is a broad-spectrum antibiotic.
▪ home remedy for acne.
▪ hair loss problems
10. How does it work?
▪ Garlic produces a chemical called
Allicin.
▪ Allicin also makes garlic smell.
16. Alkaloids
▪ [200 mg plant material boiled in 20 ml of 1% H2SO4in 50%
ethanol, filtered; filtrate + 5 drops conc.
NH4OH + 20 ml chloroform and the two layers
separated. Chloroform layer was
▪ extracted with 20 ml dilute H2SO4. Extract + 5 drops
of Mayer’s/Wagner’s/Dragendorff’s reagents, a
creamy/brownish-red/orange-
▪ red precipitate indicates the presence of alkaloids
▪
17. carbohydrates
[(500 mg plant material boiled in 30 ml distilled water, filtered);
1 ml filtrate + 1 ml of Molisch’s reagent +1 ml conc. H2SO4. A reddish ring indicates
the presence of carbohydrate
18. 1 ml filtrate + 2 ml of
Fehling’s solution + boiled for 5 min. A brick red precipitate indicates the presence of
reducing sugars.
1 ml filtrate + 1 ml Barfoed’s reagent + heat. A red precipitate indicates the presence of
monosaccharide .
19. Tannins
▪ [2 ml filtrate + 1 ml FeCl3, blue-black or greenish-black precipitate
indicates tannins; 1 ml filtrate, + 3 drops of lead sub acetate, a
colored precipitate indicates the presence of tannins
21. Flavonoids
▪ [Shinoda’sTest: (200 mg plant material in 5 ml ethanol, filtered) 1 ml
filtrate, + magnesium ribbon + conc. HCl a pink or
red color indicates the presence of flavonoids].
22. Terpenes/steroids
▪ [Liebermann - Burchard’sTest: (200 mg plant material in 10 ml
chloroform, filtered; 2 ml filtrate + 2 ml acetic anhydride + 1 ml of
conc. H2SO4. A blue – green ring indicates the presence of
terpenes/steroids
23. ▪ Anthraquinones [Borntrager’s test: 100 mg of powdered plant in 5 ml
of chloroform, filtered. 2 ml filtrate + 2 ml 10% NH4OH. A bright pink
colour indicates the presence of anthraquinones.
▪ Modified Borntrager’s test: 200 mg plant material boiled in 5 ml 10%
HCl, filtered. Filtrate extracted with 5 ml benzene and benzene layer
shaken with 5 ml 10% NH4OH. A rose pink or cherry red colour
indicates the presence of anthraquinone derivatives.
24. Analysis of Alliin
Determination of Alliin and Allicin by HPLC:
▪The isolated components were analyzed by ion-pair reversed –phase
liquid chromatography UV detector using an octadecyl silane column
with gradient elution.
▪The procedure for allicin synthesis could be improved.The time for
HPLC analysis of allicin was shortened by the application of different
isocratic elution systems.
25. ▪ Calibration was performed by use of an allicin/silica gel adsorbate as
external standard and its allicin content could be confirmed by
different methods.
▪ L-(+)-Alliin was synthesized and applied as external standard for the
quantitative determination of alliin by HPLC.
26. Conclusion
▪ TOMEX includes high dose of natural organic Egyptian garlic powder of finest quality with
concentrated alliin level.
▪ Many studies point to the fact that the most important garlic contains alliin, an important sulphur
containing compound which is responsible for the various beneficial effects of garlic.
▪ TOMEX is an excellent way to get the garlic you need into your body without eating and smelling
garlic.
▪ Garlic is a well known natural health remedy that has long been used to treat various ailments.
▪ Garlic strengthens the immune system as well as helps to fight chest infections, coughs and
congestion. In the winter monthsTOMEX is great to and ward off colds and flu.
▪
