![URECA
Undergraduate Research Experience on CAmpus
Category: 5 Student: Chin Zheng Yang Project ID: MAE16037
School of Mechanical and Aerospace Engineering
Project Title: Decellularization of Oesophagus
Supervisor: Assoc Prof Chian Kerm Sin Co-Supervisor: Ms Sitthisang Sonthika
Incidences of oesophageal cancer is on the rise
worldwide and Asian (Indian and Chinese) are
particularly prone compared to the Europeans. Current
treatment for oesophageal cancer involves
reconstructive surgery whereby the diseased
oesophagus is surgically removed and the patient's
stomach is resected and pulled into a tube to replace the
removed organ. However the procedure is complex and
suffers from severe complications such as leakage
leading to infection to outright failure.
In this project, we are attempting to use decellularized
porcine oesophagus as a potential biological scaffold to
facilitate the regeneration of the oesophagus.
Introduction
a.a) fresh oesophagus
was cleaned
thoroughly by rinsing
in antibiotics.
a.b) Muscularis
externa layers were
removed by dissection
with a scalpel.
a.c) Mucosa tissue was
sectioned into 50 mm
lengths and treated
for 7 days in SDS.
a.d) Resulting
decellularized tissue
maintained structural
integrity and tubular
appearance.
To develop suitable decellularization process to remove
cellular components from porcine oesophagus and to
evaluate their effectiveness of the process by histology
and mechanical characteristics.
Objective
Methodology
Results
1. Histology
Cross section of paraffin-
embedded decellularized
oesophageal mucosa
stained with haematoxylin
and eosin. The small
quantity of nuclei staining
indicates nearly complete
decellularization of the
tissue. The structural
appearance of the tissue is
maintained.[1]
2. DNA quantification
DNA quantification revealed that the decellularized
oesophageal mucosa possessed significantly less DNA
content then fresh oesophageal mucosa.[3]
[1],[2],[3] Decellularized ovine esophageal mucosa for
esophageal tissue engineering, R. Ackbara, H.
Ainoedhofera, M. Gugatschkab, A. K. Saxenna,
Technology and Health Care 20 (2012) 215–223.
References
Figure 1: Esophageal tissue
engineering
Figure 2: Preparation of
decellularized ovine
esophageal mucosa
Figure 4: Production of
esophageal natural acellular
matrices [2]
Figure 3: Cross section of
decellularized esophageal mucosa
Decellularization of oesophagus
Figure 5:DNA quantification of fresh and decellularized
esophageal mucosa.
Discussions
Histological examination revealed a almost complete
removal of cells throughout the entire depth of the
esophageal mucosa while DNA quantification
demonstrated a significant reduction in DNA content
in comparison to fresh tissue. However, in order to
evaluate the potential of decellularized esophageal
mucosa for reconstruction of esophageal defects,
in vivo studies need to be conducted.](https://image.slidesharecdn.com/urecaposter2-180306133918/85/URECA-Poster-1-320.jpg)
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URECA Poster
- 1. URECA Undergraduate Research Experience on CAmpus Category: 5 Student: Chin Zheng Yang Project ID: MAE16037 School of Mechanical and Aerospace Engineering Project Title: Decellularization of Oesophagus Supervisor: Assoc Prof Chian Kerm Sin Co-Supervisor: Ms Sitthisang Sonthika Incidences of oesophageal cancer is on the rise worldwide and Asian (Indian and Chinese) are particularly prone compared to the Europeans. Current treatment for oesophageal cancer involves reconstructive surgery whereby the diseased oesophagus is surgically removed and the patient's stomach is resected and pulled into a tube to replace the removed organ. However the procedure is complex and suffers from severe complications such as leakage leading to infection to outright failure. In this project, we are attempting to use decellularized porcine oesophagus as a potential biological scaffold to facilitate the regeneration of the oesophagus. Introduction a.a) fresh oesophagus was cleaned thoroughly by rinsing in antibiotics. a.b) Muscularis externa layers were removed by dissection with a scalpel. a.c) Mucosa tissue was sectioned into 50 mm lengths and treated for 7 days in SDS. a.d) Resulting decellularized tissue maintained structural integrity and tubular appearance. To develop suitable decellularization process to remove cellular components from porcine oesophagus and to evaluate their effectiveness of the process by histology and mechanical characteristics. Objective Methodology Results 1. Histology Cross section of paraffin- embedded decellularized oesophageal mucosa stained with haematoxylin and eosin. The small quantity of nuclei staining indicates nearly complete decellularization of the tissue. The structural appearance of the tissue is maintained.[1] 2. DNA quantification DNA quantification revealed that the decellularized oesophageal mucosa possessed significantly less DNA content then fresh oesophageal mucosa.[3] [1],[2],[3] Decellularized ovine esophageal mucosa for esophageal tissue engineering, R. Ackbara, H. Ainoedhofera, M. Gugatschkab, A. K. Saxenna, Technology and Health Care 20 (2012) 215–223. References Figure 1: Esophageal tissue engineering Figure 2: Preparation of decellularized ovine esophageal mucosa Figure 4: Production of esophageal natural acellular matrices [2] Figure 3: Cross section of decellularized esophageal mucosa Decellularization of oesophagus Figure 5:DNA quantification of fresh and decellularized esophageal mucosa. Discussions Histological examination revealed a almost complete removal of cells throughout the entire depth of the esophageal mucosa while DNA quantification demonstrated a significant reduction in DNA content in comparison to fresh tissue. However, in order to evaluate the potential of decellularized esophageal mucosa for reconstruction of esophageal defects, in vivo studies need to be conducted.