Reza Khorramirouz

1. A novel technique for perfusion based decellularization
of pancreas: arterial versus pancreatic duct
cannulation
J Hashemi1, P Pasalar1, M Soleimani2, R Khorramirouz3, AM Kajbafzadeh3
1Department of Clinical Biochemistry, School of Medical Sciences, Tehran University of
Medical Sciences, Tehran, Iran. 2 Department of Hematology, School of Medical Sciences,
2Tarbiat Modares University, Tehran, Iran. 3 Pediatric Urology and Regenerative
Medicine Research Center ,Section of Tissue Engineering and Stem Cells Therapy,
Department of Pediatric Urology, Children’s Hospital Medical Center, Tehran, Iran (IR)
INTRODUCTION
Type 1 diabetes is the most common disease which is
due to the lack of β cells function .Regenerative
medicine approaches such as a bioartificial pancreas
has been proposed as potential therapeutic solutions
.Increasing pancreatic islet survival and function is a
starting point for obtaining a valuable bioartificial
pancreas. Here we present two novel decellularization
techniques in order to preserve pancreatic extracellular
matrix architecture (ECM) in addition to complete
decellularization of the organ.
METHODS
Forty rats were selected and divided into two groups.
Twenty pancreas were harvested by cannulation of
common bile duct. The second group was catheterized
by aortic patch near the origin of superior mesenteric
and celiac artery bifurcations. All the resected
pancreases were washed by normal saline. Then
different percentages of SDS-Triton based solution at
different times points were applied. The decellularized
samples by both method of cannulation were
examined by histopathological evaluation, DNA
quantification and collagen content.
RESULTS
The Histopathological evaluation showed that
perfusion base-decellularization of whole pancreas
by pancreatic artery and ductal perfusion effectively
removes cellular and nuclear material but can
retaining innate molecular and spatial framework
and stiffness with perfusable vasculature and
appropriate ECM component to mimic pancreatic
cell composition.
DISCUSSION & CONCLUSIONS
The novel strategy to produce a decellularized
pancreas by ductal and arterial access may play a
major role for pancreas tissue engineering.
Furthermore, both methods could provide a 3D-
engineered pancreas comparable to the native
pancreas.
Fig. 1: Decellularization of rat pancreas: A) after and B)
before decellularization
Fig.2: A) Hematoxylin & eosin staining of normal
pancreas, C) Decellularized pancreas, B), Trichrome
staining of normal pancreas D) Decellularized pancreas
Fig.3. Acellular pancreas imaging by ductal
cannulation
A B
C D
Fig.2. CT angiography, arteriall cannulation in control
(A) and acellular (B). Ductal cannulation in control (C)
and acellular (D)
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