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LABORATORY SAFETY
Presented by
The Office of Risk Management
Loss Prevention
PRE-TEST
1. Electrical safety should not be ignored in
the laboratory. T or F
2. Inspections of laboratories are not
necessary. T or F
3. It is acceptable to eat and drink in a
laboratory. T or F
4. Who is responsible of supplying PPE?
5. What does MSDS stand for?
Safe laboratory procedures and
training are needed for everyone!
WHY IS LABORATORY SAFETY
IMPORTANT?
 TO PREVENT:
– adverse health effects from exposure to
chemicals
– exposure to organisms, diseases, etc. in
laboratories
– laboratory equipment hazards - if not
maintained properly
LAB SAFETY COURSE OBJECTIVES
•To show importance of written laboratory
program
•To show importance of safety equipment
and personal protective equipment
•To show importance of care of equipment
Lab safety must be taught to :
– all employees, including service groups
– undergraduate students
– graduate students
– professors
– visitors
REVIEW LABORATORY
SAFETY WHEN:
 new employees
 new procedures
 a change in procedures
 new equipment
LAB SAFETY CONT..
LABORATORY PROCEDURES
MUST BE SITE SPECIFIC !
based on your lab needs,
conditions, and equipment
TYPES OF LABORATORIES
 Pathology
 Chemistry
 Biology
 Radiation
 Soils
 Concrete/Asphalt
Laboratory Policies and Procedures
Must be:
written and available
AREAS TO COVER
 General procedures or rules
 Glassware
 Material handling and care
 Equipment
 Safety equipment
 Electrical safety
 Disposal procedures
 Emergency response plans
 Inspections
General Procedures or Rules:
 usually common to all areas
General Procedures or Rules:
– no food or drink allowed in the laboratory
– no mouth pipetting
– individuals not allowed to work in the
laboratory alone
– wear required personal protective
equipment
– no smoking in laboratory
– maintain good housekeeping habits
GLASSWARE PROCEDURES
 Storage
 Proper use
 Cleaning
 Cleaning up broken glassware
 Disposal of broken glassware
Glassware Handling
Is this what your lab looks like?
Glassware handling cont
 Or does it look like this?
Laboratory Materials
 Can include
– chemicals
– plants
– animals
– pathogens
– organisms
Procedures for handling chemicals
– proper labeling, including wastes
– proper storage
storage cabinets
store compatible chemicals together
rooms properly vented & correct temperature
Procedures for handling chemicals cont.
 Maintain a current inventory
 Purchasing procedures
 Proper handling
– use label or MSDS
– never test by taste or odor
– acids poured into water never vice versa
– take precautions and use proper equipment when
stirring or heating flammable liquids
– follow “industry standards” for labeling all
chemicals
Handling animals and plants
 Procedures for caring for animals and plants
including feeding and watering
 Procedures for cleaning cages
 Procedures for cleaning and/or decontaminating
rooms or locations
 Procedures for entering & leaving contaminated
areas
 Procedures for handling the animals or plants
 Procedures for animal bites or scratches
 Procedures for disposal to prevent spread of disease
Handling of diseases or organisms
 Allow only authorized individuals in infectious
disease laboratories
 Do not allow individuals to work alone
 Procedures for proper use of equipment and
maintenance
 Use proper containers for transportation, incubation,
and storage
 Labeling of laboratories and cultures
 Proper disinfecting procedures
 Hygiene procedures
 Procedures for exposure or release of material
Handling and Using Lab Equipment
– Proper installation
– Training on proper
use
– Manuals or written
procedures
available
– Inspections
– Maintenance
– DOCUMENT
EQUIPMENT INCLUDES:
 Meters
 Refrigerators
 Autoclaves
 Scales
 Hoods
 Drying oven
 Compressed gas
cylinders
 Bunsen burners
Lab Equipment
Which picture represents a proper hood?
SAFETY EQUIPMENT
– proper equipment must be available
– requires training for the location so
individuals know how and when to use
equipment appropriately
– training on the correct
maintenance &
storage is also
necessary
SAFETY EQUIPMENT
– FIRST AID AND
MEDICAL TRT
– EMERGENCY
EQUIPMENT
– SHOWERS,
EYEWASH
STATIONS
– MSDS’S
– PPE
First Aid and Medical Treatment
 First aid kit available and properly
stocked (nothing expired)
 Trained first aid attendant or
 Medical facilities within 15 minutes
 Emergency numbers posted
Emergency Equipment
 Fire blankets
 Fire extinguishers
 Emergency notification system
 Unrestricted means of communication
 Any other emergency equipment
needed for your specific laboratory
needs
Emergency Showers &
Eyewash Stations
 Immediate washing of the skin and eye
with generous amounts of water is the
most effective first aid treatment for
chemical burns (unless chemical reacts
unfavorably to water-MSDS)
Emergency Showers and Eyewash Stations
must be available
showers must be tested for proper
operation with results documented
Alternatives to Installed Showers
and Eyewashes
 Portable showers or eyewashes
 Attachments that connect to existing
faucets
– must supply at least 15 minutes of
continuous water
– must stay on until turned off
Material Safety Data Sheets, (MSDS)
– required for each
chemical
– requires employee
and student review
– must be accessable
for
employees/student
MSDS FILE
MSDS Information Includes:
 Nomenclature including chemical family
and formula
 Hazardous ingredients
 Physical data
 Fire and explosion hazard
 Health hazard
 Spill and leak procedures
 Special protection information
 Storage and handling precautions
Personal Protective Equipment
 AGENCY MUST:
– Provide PPE for all employees
at no cost to the employee
– train employees how to use PPE properly
– train employees on the limitation(s) of PPE
– train employees in proper care, storage,
and useful life, and disposal of PPE
Appropriate PPE:
– aprons,lab coats
– gloves-
latex,nitrile,neoprene
– goggles, face shields,
safety glasses
– respirators-full, partial,
dust mask
– noise protection
ELECTRICAL SAFETY
– Protection of
employees and
equipment
– inspect panels and
plugs
– GFIs (specified by
code)
– surge protectors
– inspection &
reporting programs
DISPOSAL PROCEDURES
– chemical wastes
– organisms, diseases, animals
– glassware
– spills
– sharps
DISPOSAL PROCEDURES
CONT.
 Trained persons designated to handle
disposal
 Meet all required rules and regulations
 Proper collection containers
 Waste collection contracts
 Does not expose humans, animals,
plants, etc upon disposal - may include
decontamination, sterilization,
incineration, autoclaving
EMERGENCY RESPONSE PLAN
 agencies should develop an
emergency response plan BEFORE
an emergency
 review with employees (students)
make sure they understand the plan
completely
Emergency Response Plan should
include:
–recognizing emergencies
–lines of authority
–methods of communication
–safe sites and evacuation routes
–site security and control
Emergency Response Plan should
include cont.:
–decontamination procedures
–provisions for medical treatment
–emergency alerting and response
procedures
–PPE and emergency equipment for
clean-up
–follow up
INSPECTION OF LABORATORIES
 Develop inspection report appropriate
for laboratory
 cover all areas related to laboratory
– personnel practices
– operational practices
– equipment
– emergency protection equipment
– materials inventory
– miscellaneous
WEB ADDRESS
 http://www.doa.state.la.us/orm/lp.htm
POST-TEST
1. Laboratory equipment does not require
regular maintenance. T or F
2. It is acceptable to eat and drink in the
laboratory. T or F
3. What is the most effective first aid treatment
for chemical burns?
4. Chemicals should be stored according to
compatibility. T or F
POST-TEST
5. Fume hoods should be used for chemical
storage. T or F
6. What does MSDS stand for?
7. What is some of the information an MSDS
can provide?
8. Electrical safety should not be ignored in
the laboratory. T or F
9. Name PPE that might be used in the lab.
POST-TEST
10. Who should receive lab safety training?
11. The same laboratory procedures apply to
every lab. T or F
12. Who is responsible for supplying PPE?
13. Inspections of laboratories are not
necessary. T or F
14. Chemical wastes can be disposed of in the
regular trash. T or F
POST-TEST
15. There is no need to test emergency
showers. T or F
16. Developing your emergency plan as an
emergency is occurring is the best method
of development. T or F
THANK YOU FOR ATTENDING

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Lab-Safety.ppt

  • 1. LABORATORY SAFETY Presented by The Office of Risk Management Loss Prevention
  • 2. PRE-TEST 1. Electrical safety should not be ignored in the laboratory. T or F 2. Inspections of laboratories are not necessary. T or F 3. It is acceptable to eat and drink in a laboratory. T or F 4. Who is responsible of supplying PPE? 5. What does MSDS stand for?
  • 3. Safe laboratory procedures and training are needed for everyone!
  • 4. WHY IS LABORATORY SAFETY IMPORTANT?  TO PREVENT: – adverse health effects from exposure to chemicals – exposure to organisms, diseases, etc. in laboratories – laboratory equipment hazards - if not maintained properly
  • 5. LAB SAFETY COURSE OBJECTIVES •To show importance of written laboratory program •To show importance of safety equipment and personal protective equipment •To show importance of care of equipment
  • 6. Lab safety must be taught to : – all employees, including service groups – undergraduate students – graduate students – professors – visitors
  • 7. REVIEW LABORATORY SAFETY WHEN:  new employees  new procedures  a change in procedures  new equipment
  • 8. LAB SAFETY CONT.. LABORATORY PROCEDURES MUST BE SITE SPECIFIC ! based on your lab needs, conditions, and equipment
  • 9. TYPES OF LABORATORIES  Pathology  Chemistry  Biology  Radiation  Soils  Concrete/Asphalt
  • 10. Laboratory Policies and Procedures Must be: written and available
  • 11. AREAS TO COVER  General procedures or rules  Glassware  Material handling and care  Equipment  Safety equipment  Electrical safety  Disposal procedures  Emergency response plans  Inspections
  • 12. General Procedures or Rules:  usually common to all areas
  • 13. General Procedures or Rules: – no food or drink allowed in the laboratory – no mouth pipetting – individuals not allowed to work in the laboratory alone – wear required personal protective equipment – no smoking in laboratory – maintain good housekeeping habits
  • 14. GLASSWARE PROCEDURES  Storage  Proper use  Cleaning  Cleaning up broken glassware  Disposal of broken glassware
  • 15. Glassware Handling Is this what your lab looks like?
  • 16. Glassware handling cont  Or does it look like this?
  • 17. Laboratory Materials  Can include – chemicals – plants – animals – pathogens – organisms
  • 18. Procedures for handling chemicals – proper labeling, including wastes – proper storage storage cabinets store compatible chemicals together rooms properly vented & correct temperature
  • 19. Procedures for handling chemicals cont.  Maintain a current inventory  Purchasing procedures  Proper handling – use label or MSDS – never test by taste or odor – acids poured into water never vice versa – take precautions and use proper equipment when stirring or heating flammable liquids – follow “industry standards” for labeling all chemicals
  • 20. Handling animals and plants  Procedures for caring for animals and plants including feeding and watering  Procedures for cleaning cages  Procedures for cleaning and/or decontaminating rooms or locations  Procedures for entering & leaving contaminated areas  Procedures for handling the animals or plants  Procedures for animal bites or scratches  Procedures for disposal to prevent spread of disease
  • 21. Handling of diseases or organisms  Allow only authorized individuals in infectious disease laboratories  Do not allow individuals to work alone  Procedures for proper use of equipment and maintenance  Use proper containers for transportation, incubation, and storage  Labeling of laboratories and cultures  Proper disinfecting procedures  Hygiene procedures  Procedures for exposure or release of material
  • 22. Handling and Using Lab Equipment – Proper installation – Training on proper use – Manuals or written procedures available – Inspections – Maintenance – DOCUMENT
  • 23. EQUIPMENT INCLUDES:  Meters  Refrigerators  Autoclaves  Scales  Hoods  Drying oven  Compressed gas cylinders  Bunsen burners
  • 24. Lab Equipment Which picture represents a proper hood?
  • 25. SAFETY EQUIPMENT – proper equipment must be available – requires training for the location so individuals know how and when to use equipment appropriately – training on the correct maintenance & storage is also necessary
  • 26. SAFETY EQUIPMENT – FIRST AID AND MEDICAL TRT – EMERGENCY EQUIPMENT – SHOWERS, EYEWASH STATIONS – MSDS’S – PPE
  • 27. First Aid and Medical Treatment  First aid kit available and properly stocked (nothing expired)  Trained first aid attendant or  Medical facilities within 15 minutes  Emergency numbers posted
  • 28. Emergency Equipment  Fire blankets  Fire extinguishers  Emergency notification system  Unrestricted means of communication  Any other emergency equipment needed for your specific laboratory needs
  • 29. Emergency Showers & Eyewash Stations  Immediate washing of the skin and eye with generous amounts of water is the most effective first aid treatment for chemical burns (unless chemical reacts unfavorably to water-MSDS)
  • 30. Emergency Showers and Eyewash Stations must be available showers must be tested for proper operation with results documented
  • 31. Alternatives to Installed Showers and Eyewashes  Portable showers or eyewashes  Attachments that connect to existing faucets – must supply at least 15 minutes of continuous water – must stay on until turned off
  • 32. Material Safety Data Sheets, (MSDS) – required for each chemical – requires employee and student review – must be accessable for employees/student MSDS FILE
  • 33. MSDS Information Includes:  Nomenclature including chemical family and formula  Hazardous ingredients  Physical data  Fire and explosion hazard  Health hazard  Spill and leak procedures  Special protection information  Storage and handling precautions
  • 34. Personal Protective Equipment  AGENCY MUST: – Provide PPE for all employees at no cost to the employee – train employees how to use PPE properly – train employees on the limitation(s) of PPE – train employees in proper care, storage, and useful life, and disposal of PPE
  • 35. Appropriate PPE: – aprons,lab coats – gloves- latex,nitrile,neoprene – goggles, face shields, safety glasses – respirators-full, partial, dust mask – noise protection
  • 36. ELECTRICAL SAFETY – Protection of employees and equipment – inspect panels and plugs – GFIs (specified by code) – surge protectors – inspection & reporting programs
  • 37. DISPOSAL PROCEDURES – chemical wastes – organisms, diseases, animals – glassware – spills – sharps
  • 38. DISPOSAL PROCEDURES CONT.  Trained persons designated to handle disposal  Meet all required rules and regulations  Proper collection containers  Waste collection contracts  Does not expose humans, animals, plants, etc upon disposal - may include decontamination, sterilization, incineration, autoclaving
  • 39. EMERGENCY RESPONSE PLAN  agencies should develop an emergency response plan BEFORE an emergency  review with employees (students) make sure they understand the plan completely
  • 40. Emergency Response Plan should include: –recognizing emergencies –lines of authority –methods of communication –safe sites and evacuation routes –site security and control
  • 41. Emergency Response Plan should include cont.: –decontamination procedures –provisions for medical treatment –emergency alerting and response procedures –PPE and emergency equipment for clean-up –follow up
  • 42. INSPECTION OF LABORATORIES  Develop inspection report appropriate for laboratory  cover all areas related to laboratory – personnel practices – operational practices – equipment – emergency protection equipment – materials inventory – miscellaneous
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  • 48. POST-TEST 1. Laboratory equipment does not require regular maintenance. T or F 2. It is acceptable to eat and drink in the laboratory. T or F 3. What is the most effective first aid treatment for chemical burns? 4. Chemicals should be stored according to compatibility. T or F
  • 49. POST-TEST 5. Fume hoods should be used for chemical storage. T or F 6. What does MSDS stand for? 7. What is some of the information an MSDS can provide? 8. Electrical safety should not be ignored in the laboratory. T or F 9. Name PPE that might be used in the lab.
  • 50. POST-TEST 10. Who should receive lab safety training? 11. The same laboratory procedures apply to every lab. T or F 12. Who is responsible for supplying PPE? 13. Inspections of laboratories are not necessary. T or F 14. Chemical wastes can be disposed of in the regular trash. T or F
  • 51. POST-TEST 15. There is no need to test emergency showers. T or F 16. Developing your emergency plan as an emergency is occurring is the best method of development. T or F
  • 52. THANK YOU FOR ATTENDING

Editor's Notes

  1. False. Some equipment does need regular inspections False. Nor is it ok to put on makeup Immediate washing with generous amounts of clean water True. Read the label if any questions
  2. False. They are not for storage. Material Safety Data Sheet Chemical family name, fire and explosion hazard, health hazards, PPE requirements, spill and leak procedures, storage and handling procedures, special protection information. True Aprons, gloves, goggles, face shields, safety goggles, respirators, noise protection
  3. Everyone, employees, students (graduates, and undergraduates). Professors, and visitors. False, they need to be specific The agency False Trick question. Depends upon the chemical waste. Read the label.
  4. False False. Develop it and practice it.