ATMA RAM MEENA Ph.D HORICULTURE SKRAU, BIKANER

1. An
Presentation
On
BREEDING OF TOMATO
by: Atma Ram Meena
Ph.D SCHOLAR
College of agriculture, Bikaner

3. Introduction
Among the vegetables maximum attempts
have been made to improve this crop because
of its short duration, easy cultivation and large
number of seeds per fruit and this has made it
a. n ideal crop for many research workers.
An exclusive Tomato Genetics Co-operative
located in University of California, USA,
andpublished
maintains
genetic information on crop
a large number of collections which
have already been thoroughly catalogued.

4. Origin and distribution
Tomato has its origin in Peru, Equador and Bolivia on
the basis of availability of numerous wild and
cultivated relatives of the tomato in this area.
 From its centre of origin, the tomato first moved
to Mexico for domestication and cultivation
Area
In India
74.62 lakh
and production
tomato grown in an area 4.58 lakh ha. And
tonns respectively with a productivity of
16.29 tonns / ha. The leading producing states are
UP, Karnataka, Maharashtra, Gujrat, Punjab, Haryana
and Bihar.

5. Uses
•Tomato is also rich in medicinal value.
•The pulp and juice are digestible, mild aperients, a promoter
of gastric secretion and blood purifier.
•It is reported to have antiseptic properties against intestinal
infestations.
•In the present days, it is gaining more medicinal importance
because of the antioxidant property of ascorbic acid and
lycopene content.
•It is also an important source of -carotene and valued for
their colour and flavour.
•Thus, today it is one of the important raw materials for
multimillion food industries. Tomatoes are also called as “Poor
man’s apple”.
•Tomato is consumed either fresh, cooked or processed into
various products like juice, ketchup, sauce, puree, whole
canned fruit and is the main constituent of ‘pizzas’.

6. TAXONOMY
Family- Solanceae
Linnaeus called it Solanum lycopersicon.
Miller: Lycopersicon esculentum
Now restored as Solanum lycopersicon. Lykos=
Wolf, persicon= peach.

7. Lycopersicon: (1) Eulycopersicon- Red
fruited edible species with carotenoid
pigmentation, annual and self compatible.
E.g.
pimpinellifolium
L. esculentum and L.
(2)
with
Eriopersicon-
anthocyanin
Green fruitedspecies
pigmentation and self
incompatible.
E.g. L. hirsutum, L. peruvianum, L.
cheesmani, L.chilense

8. L. cerassiformae is the immediate proginator
and L.hirsutum is ultimate proginator.
 Interspecific crosses between L. esculentum
and L. pimpinellifolium are easily made.
 Embryo abortion may occur between L.
esculentum x L. peruvianum. However, this can
be overcome through embryo rescue technique.
 Chromosome Number: True diploid, 2n= 24.
All species of Lycopersicon have 2n=24.

9. BOTANY
is characterized by two types of plant:Tomato plant
Determinate
frequently in
type – inflorescence occurs more
almost every internode until terminal
ones are
In other
the main
formed and elongation ceases at this point.
words, it may be defined as self-topping and
axis terminates with a flower cluster.
Indeterminate type- inflorescence cluster occurs at
every third internode and the main axis continues
growing indefinitely.

12. Flowers are borne in small forked raceme
cyme. There are four to eight flowers in each
compound
perfect,
inflorescence.
hypogynous.
Flowers
There
are
is
pendent,
a light
protective anther cone surrounding the stigma
leading to self-pollination
Dehiscence of anther is longitudinal, 1-2 days
after opening of corolla.
 If the pollen is shed as the style grows up
through anther tube,
stigma
self-fertilization occurs
and when the protrudes, chances of
cross-pollination through bees increases.

13. The optimum temperature for pollination is
around 21 C.
Emasculation is usually done 1 day prior to
anthesis/flower opening.
At this stage, the sepals have started to
areseparate
beginning
and the anthers and corolla
to change from light to dark yellow.
 The stigma is fully receptive at this stage
allowing for pollination even immediately after
emasculation.
 Protection of pollinated flowers by wrapping
with cotton or small pollination bags is
essential.

16. BREEDING
Earliness
OBJECTIVES



Increased fruit yield
Fruit quality:
Large round fruit with adequate firmness and
andshelf life, uniform fruit size, shape, red colour
freedom
for fresh
Large
from external blemishes or abnormalities
market
fruit size, high fruit quality and continuous
production for home garden tomatoes
Deep, uniformly red coloured tomatoes, pH below
4.4, high TSS (4.5-7%) and high alcohol insoluble
solids (AIS) in processing tomatoes

17. Indeterminate
production
cultivars for greenhouse
Resistance to diseases:
Fusarium wilt (Fusarium oxysporum f.sp.
lycopersici (Sacc.)
Verticillium
Berth)
wilt (Verticillium albo-atrum Reinke &
Late blight (Phytophthora infestans)
Early blight (Alternaria solani)
Septoria leaf spot (Septoria lycopersici)
Anthracnose (Colletotrichum phomoides)
Bacterial wilt (Pseudomonas solanacearum)
Bacterial canker (Corynebacterium michiganense)
Tomato mosaic (Tobacco mosaic virus)
Root knot nematode (Meloidogyne incognita)

18. Resistance to insects:
Fruit borer (Heliothis armigera)
White fly(Bemisia tabaci)
 Resistance to abiotic stresses:
Cold set varieties
Hot set varieties
Drought tolerance
Salt tolerance
Low temperature germination
Chilling injury tolerance
Herbicide tolerance
and growth

19. BREEDING METHODS
1. INTRODUCTION: It consists of taking a
genotype
into new
or a group of genotypes of plants
environments where they have not
been grown before.
Ex. Sioux from USA, Marglobe from USA.

20. 2. PURE LINE SELECTION: A pure line is
progeny of single homozygous plant of self
pollinated species. Therefore, all the plants in
a pure line have the same genotype.
Ex.Arka
(USA)
Arka
Breeding
Arka
Vikas: (+ Rainfed), PLS from Tip-Top
Saurabh:
line)
PLS from V-685 (Canadian
Abha: (+bacterial wilt), PLS from VC-
8-12-1 (AVRDC, Thaiwan)
Arka Alok: (+bacterial wilt), PLS from CL-
144-5-1-0 (AVRDC, Thaiwan)
Arka Ahuti: (+processing), PLS from
Ottawa-60 (Canada)

21. 3. SINGLE SEED DESCENT METHOD: Suited for
characteristics with low heritability (yield & yield
components,
spacing.
earliness) and in crops with wider
•SSD
•This
from
is a modification of bulk method.
of having a single seedis the
each
classic procedure
plant, bulking the individual seeds, and
planting out the next generation.
•With this procedure one or two seed
bulk
are
to
collected
from each F2 plant and then grow F3
generation
•SSD is a system to rapidly develop pure lines
followed by selection among those pure lines

22. 4. SINGLE PLANT SELECTION: single plant
seletion is used to select chance variants, off types,
superior types from mixed homozygous population in
of
of
self pollinated vegetable crops. This is one
useeffective
germplasm.
method to make maximum
Eg: Earliana, Greater Baltimore and Bonny Best.
5. MASS SELECTION: Massing of IIHR-674 from
VC-82 line
(+ P.M. and
from USA developed in to Arka Ashish
processing ).

23. 6. Pedigree Method: Pedigree may be defined
as a description of the ancestors of an
individual and it generally goes back to some
past.distant
Pedigree
ancestor or ancestors in the
would describe the parents, grand-
parents, great grand parents and so on.
 It is useful by finding out if two individuals
are related by descent, have a common parent
in their ancestors and they have some genes in
common.
Eg: Arka Meghali of tomato PS (F8) of Arka
Vikas x IHR 554
Pusa Ruby selection from Improved
Meeruti x Sioux

24. 7. Back Cross Method: A cross between a
hybrid (F1 or segregation) and one of its
parents is known as back cross. Hybrid
subsequentand the progenies in the
generation are repeatedly back crossed to one
of the parents.
 Followed when character is governed by
one/few genes.
 For Early blight, Nematode and Bacterial
wilt resistance in tomato.

25. 8. Heterosis Breeding:
Though, tomato a self pollinated crop has a tremendous
potential for heterosis breeding. The commercial exploitation
of hybrid vigour in tomato has received greater importance on
account of several advantages of hybrids over pure line
varieties with response to marketable
resistance
fruit yield and its
component traits as well as to biotic and abiotic
stresses. That is why large number of commercial hybrids have
been developed in the country in this crop. With increasing
popularity of F1 hybrids in tomato, it is imperative to obtain
such hybrids which have excellent qualities
with resistance to diseases.
and yield coupled
Released F1 Hybrids:
Arka
Arka
Arka
Arka
Vishal (IHR-837 X IHR-932)
Vardan: IHR-550-3
Shreshta: 15-SBSB
Abhijit: 15-SBSB X
X IHR-932
X IHR-1614
IHR-1334

26. 9. Interspecific Hybridization:
L. peruvianum – Source of Resistance to
Verticillium wilt, TLCV, Nematodes and
Salinity.
L. pimpinellifolium – Source of Resistance
to bacterial wilt, Fusarium wilt. Source of
carotene and Vit-C content.
L. hirsutum - Source of Resistance to
Fusarium wilt, Helicoverpa insect and Virus.
L. cheesmani -
resistance.
L. pennelli -
salinity resistance.
Good source for salinity
Drought resistance and

27. 10. Mutation
S12
Breeding:
(Pusa S12 ) – Sioux variety is exposed to
mutation.
Pusa Lal Meeruti – From Improved
Meeruti.
 Seeds were exposed to 15-30 Kv. Of ϒ-rays.
Natural mutants are also available in tomato like-
Nr
rin
- (Never ripening)
– Single recessive gene (Ripening
inhibitor)
(Non-ripening)
(Slow ripening)
nor
alc
–
-

28. 11. Polyploidy Breeding:
Not so much scope in tomato 0.2% Colchicine-
Tetraploids are sterile and fruit size was smaller.
12. Biotechnology:
(i)Antisense RNA for Polygalactourinase (PGU) enzyme.
(The cell wall degrading enzyme. Activity appears at the onset
of ripening and in ripe fruit it is one of the major cell-wall-
bound proteins.) Eg. FLAVR SAVR Variety available in
market.
(ii) Viral Coat Protein – Introduced gene which can
synthesize the protein which coats the virus material. Eg.
Avinash-1 and Avinash-2 varieties.
(iii) Herbicide Resistance:- Gene which can resist that
herbicide. Means variety should not be affected but weeds
should be cleared.
Insect Resistance: Eg. ‘Bt’
resistance.
for Helicoverpa(fruit
borer)

30. Breeding
Processing
Fruits with high
for
type
Storage life and
acidity, uniform intense red colour, high TSS,
Resistance to cracking, elongated fruits with high yield. Plants
should be adopted to mechanical harvesting with determinate
habit, concentrated fruit set for once harvesting only and
jointless pedicel on fruit etc.
Varieties – Roma, Punjab Chuhara, Arka Ahuti, Arka
Juice and Canning purpose:
Roma, Son Marzano, Red Top, HS-110
Puree preparation:
Indo Process-II, Indo Process-III, Rupali
Paste and Ketchup:
HS 101, HS 110.
Ashish

31. Breeding for Resistance to Diseases
Bacterial wilt: Arka Abha, Arka Alok, PI 127805-A
(L.pimpinellifolium)
Leaf Curl Virus : L. peruvianum & L. hirsutum.
Resistant Varieties: Nandi, Vaibhav, Sankranthi.
Nematodes: ‘Mi’ gene carries resistance to nematodes,
located on chromosome No. 6 in tomato.
PI 128657 (L.peruvianum), Neematex (not infected by
all three races of RKN),
PNR-7, Pusa-120 etc.
Arka Vardan (F1 hybrid), NTDR-1,
Hybrids :
Determinate type: ARTH-3, Pusa Hybrid-2, NARF-101,
Swarna-12, NDTH-6, Nath Amruth-501, DTH-4
Indeterminate type: ARTH-4, FM-2, KT-4, MTH-6,FM-1, Nath
Amruth-601, NDTH-2.

32. Other hybrids
Pusa Divya – Indeterminate type
Pusa Sadabahar- It can set fruit at wide range of
temperature (6-30⁰C).
Kashi Hybrid-1 and Kashi Vishesh (H-86) – from IIVR,
Varanasi
Pusa Hybrid-8: Determinate hybrid from IARI, New
Delhi.
Arka Ananya: Semi-determinate and resistant to leaf
curl virus.

33. Varieties
Pusa Red Plum
•It has been evolved at the IARI by a cross between a
cultivated tomato and the wild L .pimpinellifolium.
•It is an early variety suitable for table use.
•The fruits are small, borne in clusters with red colour and
high vitamin C and sugar content.
•The plants are tall, with determinate growth habit, vigorous
and high yielding.
Pusa Early Dwarf
•It is an early ripening selection from the cross between
'Improved Meeruti' and 'Red Cloud' - a typical dwarf type
with medium large fruits of uniform colour.
•It has been observed to do well in both the seasons, and
yields up to 395 q/ha.

34. Pusa Early Dwarf
•It is an early ripening selection from the cross
between 'Improved Meeruti' and 'Red Cloud' - a
typical dwarf type with medium large fruits of
uniform colour.
•It has been observed to do well in both the seasons,
and yields up to 395 q/ha.
CO 2
•It is a selection from a Russian introduction. It is an
indeterminate variety.
•The fruits are flat with 4-5 furrows.
•Each fruit weighs on an average 55-60 g.
•Ripe fruits are deep orange red in colour, its
duration is 140 days and the yield is 28-30 tonnes/ha.

35. CO 3 (Marutham)
•It is an induced mutant from Co 1; a determinate type
suitable for close planting even as close as 30 cm x 30 cm.
•Fruits are round in shape with four
red in colour.
•It is a cluster bearing type capable
tonnes/ha under a close spacing in a
light grooves and capsicum
of yielding as high as 40
duration of 100-105 days.
PKM 1
•This is another induced mutant from a local variety called
Annanji.
•The fruits are flat-round with prominent green shoulder and
capsicum red colour.
•It is suitable for long distance transport.
•It yields about 30-35 tonnes/ha in a crop duration of 135
days.

36. Arka Saurabh
•Developed by Indian Institute of Horticultural
Research, Bangalore, it is a high-yielding variety
exhibiting 30-35% higher yield than "Pusa Ruby'.
•It is semi-determinate, bears very firm, deep red,
medium-sized fruits with smooth skin and thick flesh.
•The fruits are round with nipple tip.
•It has very good transport and keeping quality, 8-10
days at room temperature amongst the round-fruited
varieties.
•It has an excellent blend of sugar and acidity and is
very suitably both for
•fresh and processing purposes particularly for
ketchup and juice-making.

37. Arka Ahuti (Sel.11)
•It is pureline selection from 'Ottawa-60' developed at IIHR,
Bangalore.
•Plants are semi-determinate with a duration of 135 days.
•The fruits are oblong, thick fleshed, bilocular and uniform
ripening.
•The fruits have a TSS of 5.25% with an acidity of 0.35%.
•It has an yield potential of 45 tonnes/ha.
Arka Abha (BWR-1)
•This is from line selection (IHR 638-12) from a SSD derived
line got from AVRDC, Taiwan (VC8-1-2-1).
•The semi determinate vines bear oblate fruits with an
average fruit weight of 75 g with light green shoulder.
•It is resistant to bacterial wilt and is capable of yielding 25
tonnes/ha. in 135 days.

38. Arka Ashish (IHR 674 SBSB)
•Developed at IIHR, Bangalore.
•It is an improvement over UC83 B from California.
•The plants are determinate with concentrated fruit maturity.
•The fruits are uniform ripening, very firm, bilocular, oval
the ripened fruits store well on the vine.
•It is tolerant to powdery mildew. The colour is excellent
(Lycopene 10 mg/100g), TSS is 4.8% and with low acidity
(0.35%), it makes excellent ketchup, juice and puree.
•It yields 35 tonnes/ha. in 130 days
and
HS101
•Developed at Haryana Agricultural University, Hisar by
thehybridization (Sel 2-3 X Exotic culture) and selecting in
segregating progenies.
•The plants are determinate, dwarf, fruit medium sized, round,
borne in cluster of 2-3, juicy, suitable for winter season for
northern region.

39. Hisar Arun (Sel-7)
•Developed at HAU, Hisar, by hybridization and
selection from a cross Pusa Early Dwarf X K1.
•Extremely early and high yielding, plants are
determinate, dwarf in stature with concentrated
flowering and fruiting; fruits medium to large, round,
deep red, recommended for cultivation in Delhi, Uttar
Pradesh, Karnataka, Orissa and Maharashtra.
Hisar Lalima (Sel-18)
•Developed from the segregating population of a
cross Pusa Early Dwarf X HS101 at HAU, Hisar.
•An early variety. Plants are determinate, leaves have
deep cut, fruits are round, large, fleshy, high yielding

40. Arka Alok (BWR-5)
•It is also a bacterial wilt resistant variety developed through
pure line selection (IHR 719-1/6) out of an accession (CL114-5-
1-0) from AVRDC, Taiwan developed at IIHR, Bangalore.
•The plant habit is determinate with a duration of 125 days.
•The fruit weighs on an average of 100 g and is square round
shape, thick fleshed with light green shoulder.
•It has an yield potential of 35 tonnes/ha.
in
HS102
•Developed at HAU, Hisar by selecting from the segregating
progenies of the cross S12 X Pusa Early Dwarf.
•It is an early variety with concentrated flowering and
fruiting.
•Fruits medium to small, round, juicy, thin pericarp,
recommended for cultivation in Haryana.

41. Hisar Lalit (NRT 8)
•Developed at HAU, Hisar by bybridization and
selection from a cross HS101 X Resistant Bangalore.
•The plants are semi-determinate, early with round
medium to large fruits, resistant to root-knot
nematode.
•Recommended for
infested area.
cultivation in root-knot nematode
Hisar Anmol (H24)
•It
leaf
is a variety showing field resistance to tomato
throughcurl virus.
from
Developed at HAU, Hisar
selection the cross Hisar Arun X L.hirsutum
f.glabratum.
•Plants are determinate, fruits medium in size, round,
red and fleshy.

42. Pant T3
•Another variety developed through pure line selection at
G.B.Pant University, Pant Nagar.
•Plants are semi-determinate, stem thick, round, hairy.
•Suitable for winter season, fruits medium, round and smooth.
•Recommended for Delhi, Uttar Pradesh, Orissa and
Maharashtra
Arka Meghali
•It is a new high yielding tomato variety developed at IIHR,
Bangalore through pedigree method of selection from a cross
between Arka Vikas and IHR 554 specifically suited for
rainfed cultivation.
•Under rainfed situation it recorded on an average 17.2
tonnes/ha compared to 10.9 tonnes/ha by Pusa Ruby.
•The individual fruit weight is 63 g. It has more thick and firm
flesh than Pusa Ruby.

43. Cherry TomatoRoma Variety