1. Objective 4- CHICKPEA

2. Activities
Activity 1: Utilise genetic diversity to develop breeding
and MAGIC populations (Harnessing diversity)
Activity 2: Develop genomic resources for enhancing MABC
and MARS activities (Genomic resources)
Activity 3: Employ MABC and MARS activities to improve
superior lines (Drought tolerance breeding)
Activity 4: Strengthen capacity of NARS partners
(Capacity building)
Activity 5: Management and storage of data
(Managing data)

3. Activity 1: Harnessing Diversity
Milestones:
1. 8 Parental genotypes for generating pre-breeding populations
and MAGIC populations identified
COMPLETED
2. At least 2 pre-breeding populations (F2) generated (Milestone for year 2)
Pre-breeding populations will be advanced
3. Phenotyping of pre-breeding populations completed and at least 10 pre-
breeding lines identified for TLII (Milestone for year 4)
✔4. 500 MAGIC lines made available for (Milestone for year identification of
superior lines for drought tolerance
trait mapping and
3)
1200 lines will be advanced to F6 and genotyped
5. Phenotyping data for drought-related traits made available on selected sets of
at least 200 MAGIC lines for utilization in breeding and marker/gene
discovery for drought tolerance (Milestone for year 4)

4. Breeding populations to be
advanced (Milestone 2)
Crosses made in India: 17
Crosses in F4 :4
Crosses in F5 :1
Crosses in F6 :9
Crosses in F7 :3
Crosses made in ICRISAT Nirobi: 20
Crosses in F2 : 20
Crosses made in EU Kenya: 2
Crosses in F2 :2
Crosses made in EIAR Ethiopea: 5
Crosses in F2 :2
Crosses in F3 :3

5. MAGIC populations to be
advanced (Milestone 4)
8 parents: A) ICC 4958, B) JAKI 9218, C) JG 130, D) ICCV 00108,
E) ICCV 97105, F) ICCV 10, G) JG 11, H) JG 16
28 2-ways
Oct 09 – Feb 10
Field
14 4-ways
Jun 10- Sep 10
Green house
7 8-ways
Oct 10-Feb 11
Field
F1s raised and selfed in green house Mar 11- Jun 11
F2s raised and selfed in green house Jun 11- Sep 11
SSD method
1200 F3 progenies raised in field Oct 11- Feb 12
SSD method
1200 F4 progenies raised in field Feb 12- May 12
SSD method
SNP genotyping 1200 F5 progenies will be raised in field Jun 12- Sep 12
SSD method
1200 F6 progenies will be raised in field Oct 12- Feb 13

6. Activity 2: Genomic resources
Milestones:
6. At least 768 informative SNPs for cultivated germplasm compiled
COMPLETED
7. High-throughput and cost-effective SNP genotyping platform for at least
768 SNPs made available
COMPLETED
8. At least 5 candidate genomic regions identified for developing local physical
maps
COMPLETED
9. Integrated QTL and physical map made available for selected 5
genomic regions
Efforts were initiated to develop genome-wide physical map for chickpea
10. Sequence data for selected BAC contigs for 5 genomic regions generated
✔ (Milestone for year 3)
11. 4 Additional markers from each of selected 5 QTL regions generated
✔ (Milestone for year 3)

7. Sequencing of BAC contigs
and development of markers
(Milestone 10 and 11)
 Genome assembly to be available in next four
months
 With the help of genetic map, sequence from
BAC ends, together with genome assembly
new markers will be developed for target
QTL regions

8. Activity 3: Drought Tolerance Breeding
Milestones:
12. Phenotyping data collected and appropriate phenotyping methodology(ies) selected based
on detailed analysis of phenotyping data (Milestone for year 2)
Detailed phenotyping analysis will be conducted and appropriate methodologies selected
✔At least 6 candidate markers varieties andtolerance identified (Milestone for year 3)
13.
14. At
least 5
farmer-preferred
for drought
donor genotypes identified COMPLETED
15. MABC programme being run by each NARS partner from Ethiopia, Kenya and India
✔ (Milestone for year 2) MABC to be run by NARS
16. MABC products from Phase I evaluated by each NARS partner (Milestone for year 3)
✔ MABC to be evaluated by NARS
17. At least 20 homozygous plants from BC F progenies (MABC) of Phase II selected
3 2
✔ (Milestone for year 3)
18. 3 cycles of recombination completed for MARS (Milestone for year 3)
✔ 2-4 farmer-preferred cultivars developed through MABC, and at least 10 superior lines
19.
1 cycle of recombination initiated
st
improved for drought tolerance for SSA and Asia through MARS
20. At lest 2 breeding populations of TLII genotyped for drought and FW-resistance markers
(Milestone for year 4)
25. A proposal to leverage funding for chickpea drought tolerance molecular breeding in South Asia
approved by the Indian Government for a period of 3-4 years (200K/year)
COMPLETED

9. At least 5 candidate markers for
drought tolerance identified
(Milestone 13)
 Based on genome-wide association mapping and
mining the genome sequence, candidate markers
associated with drought tolerance will be
identified

10. MABC by NARS
(Milestone 15)
NARS Cross Plan (2012-13)
partner
EIAR, Ejere × ICC 4958 BC3F3 seeds will be harvested
Ethiopia
Arerti x ICC 4958 Seed miltiplication of 16 BC3F3
lines
EU, Kenya ICCV 97105 × ICC 4958 BC3F3 seeds will be harvested
ICCV 95423 × ICC 4958 6 BC3F4 lines will be evaluated
IIPR & ICCV 10 × ICC 4958 20 BC3F4 lines will be evaluated
IARI, India
IIPR, India DCP92-3 × ICC 4958 BC3F1 seeds will be harvested
KWR108 × ICC 4958 BC2F1 seeds will be harvested
IARI, India Pusa 362 × ICC 4958 BC3F1 seeds will be harvested

11. Evaluation of MABC lines of
phase 1
(Milestone 16)
Activity Location
BC3F5 lines of EIAR, Ethiopia
JG 11 × ICC 4958 (22 lines) Egerton University, Kenya
ICRISAT, Nairobi
ICRISAT, Patancheru
TL II partners
BC3F5 lines of EIAR, Ethiopia
1. Chefe × ICC 4958 (8 lines) Egerton University, Kenya
2. KAK2 × ICC 4958 (2 lines) ICRISAT, Patancheru
TL II partners

12. Recombination cycles planned
for MARS (Milestone 18)
JG 130 X ICCV 05107- Egerton Uni, Kenya; IIPR, India
JG 11 X ICCV 04112- EIAR, Ethiopia; IARI, India
A × B C × D E × F G × H 10 seeds sown for each line
Recombination cycle I
Min 20 seeds from each cross
AB × CD EG × GH Marker analysis-
select 10 seed and sow
Recombination cycle II
Min 100-200 seeds
Harvested from each
ADCD × EFGH Cross
Marker analysis-
select 10 seed and sow
Recombination cycle III
ABCDEFGH Select best lines based on allele from all 8 lines
Self heterozygous lines
Select best lines based on evaluation

13. Activity 4: Capacity building
Milestones:
21. One modern breeding workshop organized for TLI and
TLII breeders
COMPLETED
22. 4 MSc and 2 PhD students trained in chickpea genomics
and breeding activities (Milestone for year 4)
Work of 4 PhD and 2 MSc students will be continued

14. Modern molecular breeders
for NARS (Milestone 12)
 Kenya:
- Ms Serah Songok, PhD student, Eger Uni
(Supervisors: P Kimurto, R Mulwa, RK Varshney)
- Ms Alice Koskie, PhD student, WACCI
(Supervisors: JK Kedawi, P Kimurto and PM Gaur)
- Mr Moses Oyier, MSc student, Eger Uni
(Supervisors: P Kimurto, R Mulwa, RK Varshney)
 Ethiopia:
- Mr Musa Jarso, PhD student, Addis Ababa Uni
(Supervisors: Prof Endeshaw, A Fikre, RK Varshney)
- Mr Kebede Teshome, PhD student, Haramaya Uni
(Supervisors: F Mekbib, RK Varshney, PM Gaur)
- Mr Getachew Tilahun, Addis Ababa Uni
(Supervisors: A Fikre, RK Varshney )

15. Activity 5: Managing data
Milestones:
23. At least 8 datasets comprising marker sequence data, marker
genotyping data, mapping data and phenotyping data obtained
in Phase I curated in appropriate databases
COMPLETED
24. At least 10 datasets comprising marker genotyping and/or
phenotyping data on reference collection, mapping
populations, MAGIC populations, MABC and MARS populations
obtained in Phase II curated in appropriate databases
(Milestone for year 4)

16. Thanks a lot!