Successfully reported this slideshow.
We use your LinkedIn profile and activity data to personalize ads and to show you more relevant ads. You can change your ad preferences anytime.

Accelerated chickpea breeding for water-limited environments

238 views

Published on

Chickpea is a cool season food legume largely grown on residual soil moisture, the crop often experiences moisture stress towards end of the crop season (terminal drought). The crop may also face heat stress at the reproductive stage if sowing is delayed.

Published in: Government & Nonprofit
  • Be the first to comment

  • Be the first to like this

Accelerated chickpea breeding for water-limited environments

  1. 1. Accelerated chickpea breeding for water-limited environments Srinivasan Samineni Chickpea breeding Patancheru-ICRISAT
  2. 2. Chickpea in water-limited environments • Chickpea is a cool season food legume largely grown on residual soil moisture. • The crop often experiences moisture stress towards end of the crop season (terminal drought). • The crop may also face heat stress at the reproductive stage if sowing is delayed. • The increasing climate variability. • The genetic approaches being used: development early maturing varieties with enhanced tolerance to these stresses.
  3. 3. • Early maturing varieties • Tolerance to terminal drought and heat stresses • Resistant varieties for collar rot, dry root rot and ascochyta blight diseases • Improving protein content and micronutrients • Herbicide tolerance • Suitability to machine harvesting • Improving market preferred seed traits (color, size and shape) 1. Major research focus 2. Modernization of chickpea breeding
  4. 4. Breeding for early maturity Early flowering Early flowering F1 • Few genes controlling flowering time • efl-1 (ICCV 2) • efl-2 or ppd (ICC 5810) • efl-3 (BGD 132) • efl-4 (ICC 16641/ICC 16644)* *Newly identified Gaur PM, Samineni S, et al (2015). Euphytica 203: 295-308 Mallikarjuna BP, Samineni S, et al (2017). Frontiers in Plant Science • Major genomic regions controlling early flowering • efl-1 (Qefl1-2) on CaLG04 • efl-2 (Qefl2-1) on CaLG01 • efl-3 (Qefl3-3) on CaLG08 • efl-4 (Qefl4-1) on CaLG06 • Early-phenology is an important mechanism for terminal drought stress and adaptation to new niches
  5. 5. Breeding for drought tolerance Selection based on grain yield under rainfed (moisture stress) condition
  6. 6. • A genomic region (QTL-hotspot) for root traits and several drought tolerance related traits, contributing up to 58.2% phenotypic variation identified (Varshney et al 2014) • This genomic region was introgressed into several cultivars from South Asia and sub-Saharan Africa using marker-assisted backcrossing Mapping QTLs for drought tolerance and MABC ICC 4958 × ICC1882 RILs ICC 283 × ICC 8261 RILs
  7. 7. Performance of introgression lines in JG 11 S N Entry ICRISAT Nandyal Dharwad Gulbarga 1 ICCIL 01001 4 -9 8 21 2 ICCIL 01003 -9 9 8 39 3 ICCIL 01009 -1 -1 6 -1 4 ICCIL 01010 -6 1 1 21 5 ICCIL 01011 13 -1 16 3 6 ICCIL 01012 0 -2 5 -11 7 ICCIL 01013 18 23 12 21 8 ICCIL 01015 14 -3 8 -4 9 ICCIL 01019 -4 -3 12 -23 10 ICCIL 01021 22 4 10 17 11 ICCIL 01023 1 -1 4 38 12 ICCIL 01026 -14 -6 9 19 13 ICCIL 01030 6 13 -5 7 14 ICCIL 01031 -1 4 15 3 15 ICCIL 01032 4 2 9 11 16 ICCIL 01033 -1 5 10 6 17 ICCIL 01034 8 19 2 13 18 ICCIL 01035 10 7 17 -23 19 ICCIL 01037 -13 25 -4 -35 20 ICCIL 01038 -5 20 1 -1 21 ICCIL 01040 -3 8 18 18 22 ICCIL 01041 -12 -1 15 27 Irrigated S N Entry ICRISAT Nandyal Dharwad Gulbarga 1 ICCIL 01001 27 2 13 33 2 ICCIL 01003 18 10 2 37 3 ICCIL 01009 15 4 9 0 4 ICCIL 01010 18 -14 -11 -22 5 ICCIL 01011 2 1 9 6 6 ICCIL 01012 6 9 11 -2 7 ICCIL 01013 14 33 -22 20 8 ICCIL 01015 24 2 13 -2 9 ICCIL 01019 -8 0 -10 -1 10 ICCIL 01021 3 4 -12 17 11 ICCIL 01023 1 21 -4 20 12 ICCIL 01026 -6 -5 -4 -6 13 ICCIL 01030 23 7 1 -13 14 ICCIL 01031 0 8 12 -4 15 ICCIL 01032 27 3 11 26 16 ICCIL 01033 5 10 12 1 17 ICCIL 01034 -1 -10 -1 2 18 ICCIL 01035 -2 39 -8 17 19 ICCIL 01037 16 -4 -3 5 20 ICCIL 01038 -4 -4 -17 13 21 ICCIL 01040 6 30 6 13 22 ICCIL 01041 5 -13 13 1 Rainfed Note: Values represent % increase/decrease in seed yield over the recurrent parent (JG 11); Values in bold are significant compared to JG 11
  8. 8. • Seven ILs were entered by partners in Initial Varietal Trial (IVT) of All India Coordinated Research Project (AICRP) on Chickpea during 2015-16. Three were promoted to AVT. • Ten ILs are being tested in a special Advanced Varietal Trial 1 (AVT 1) of AICRP on Chickpea during 2016-17 and advanced to AVT 2. Performance of ILs at national level testing
  9. 9. Improving heat tolerance • Reproductive phase is sensitive to heat stress, particularly the flowering stage. • Two RIL populations have been developed from two crosses (ICC 4567 x ICC 15614, ICC 4567 x ICC 1356) in chickpea. • Four major QTLs (PV 50%) for number of filled pods, number of seeds, grain yield and % pod setting, located in CaLG05 & CaLG06 genomic region identified CaLG05CaLG06 Paul P, Samineni S, et al (2017) communicated
  10. 10. Development of drought & heat tolerant lines from MAGIC population 28 two-way crosses 14 four-way crosses 7 eight-way crosses 7 F17 F1 F2F2 F3 (1200)F3 (1200) F8 (1136)F8 (1136) Single seed descent method Phenotypic evaluation A = ICC 4958 B = ICCV 10 C = JAKI 9218 D = JG 11 E = JG 130 F = JG 16 G = ICCV 97105 H = ICCV 00108 • Developed 1200 MAGIC lines using 8 cultivars/promising germplasm • Large variation among MAGIC lines for • flowering time (34-69 d), • plant height (23.3-65 cm), • seed yield (179-4554 kg/ha), • harvest index (0.10-0.88) • seed size (10-45) • Four MAGIC lines are being tested in (IVT) of AICRP on Chickpea
  11. 11. Varieties suitable for machine harvesting • In 2016, first machine harvestable variety NBeG 47 released in Andhra Pradesh, India • In 2017, Phule Vikram and RVG 204
  12. 12. Now, able to take 3 generations per year (Oct-Feb, Feb-May, and May-Sep) Recent experiments showed that 5 generations per year are possible Modernization of chickpea breeding 1 2 Rapid generation advancement 40 d 8-10 hr20-24 hr 25-30 d 45-50 d22-25 d Earliest seed stage ready for germination after flower initiation
  13. 13. Planting and harvesting by machines Digital data recording-Tablets, barcode labels Post-harvest observations: Digital seed counters, incubators, seed dryers, seed blowers etc. 1 All nurseries (55/55) and trials (101/101) conducted during 2016-17 in Breeding Management System (BMS) Modernization of chickpea breeding Mechanization 2 3 1 Data Management 4 Seed inventory: in progress Conti…
  14. 14. Thank You Chickpea mutants

×